Alleviating protein-condensation-associated damage at the endoplasmic reticulum enhances plant disease tolerance

IF 20.6 1区 医学 Q1 MICROBIOLOGY Cell host & microbe Pub Date : 2024-08-06 DOI:10.1016/j.chom.2024.07.013
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Abstract

Disease tolerance is an essential defense strategy against pathogens, alleviating tissue damage regardless of pathogen multiplication. However, its genetic and molecular basis remains largely unknown. Here, we discovered that protein condensation at the endoplasmic reticulum (ER) regulates disease tolerance in Arabidopsis against Pseudomonas syringae. During infection, Hematopoietic protein-1 (HEM1) and Bax-inhibitor 1 (BI-1) coalesce into ER-associated condensates facilitated by their phase-separation behaviors. While BI-1 aids in clearing these condensates via autophagy, it also sequesters lipid-metabolic enzymes within condensates, likely disturbing lipid homeostasis. Consequently, mutations in hem1, which hinder condensate formation, or in bi-1, which prevent enzyme entrapment, enhance tissue-damage resilience, and preserve overall plant health during infection. These findings suggest that the ER is a crucial hub for maintaining cellular homeostasis and establishing disease tolerance. They also highlight the potential of engineering disease tolerance as a defense strategy to complement established resistance mechanisms in combating plant diseases.

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减轻内质网蛋白质缩聚相关损伤可提高植物的抗病能力
耐病性是一种对抗病原体的基本防御策略,无论病原体如何繁殖,都能减轻组织损伤。然而,其遗传和分子基础在很大程度上仍然未知。在这里,我们发现内质网(ER)上的蛋白质凝结调节拟南芥对丁香假单胞菌的抗病性。在感染过程中,造血蛋白-1(HEM1)和 Bax 抑制剂 1(BI-1)通过相分离行为凝聚成与 ER 相关的凝聚体。虽然 BI-1 有助于通过自噬清除这些凝集物,但它也会将脂质代谢酶封存在凝集物中,从而可能扰乱脂质平衡。因此,阻碍凝集物形成的 hem1 或防止酶被螯合的 bi-1 基因突变能增强组织损伤的恢复能力,并在感染期间保持植物的整体健康。这些发现表明,ER 是维持细胞平衡和建立抗病能力的关键枢纽。这些发现还凸显了将工程抗病性作为一种防御策略的潜力,以补充现有的抗病机制,对抗植物病害。
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来源期刊
Cell host & microbe
Cell host & microbe 生物-微生物学
CiteScore
45.10
自引率
1.70%
发文量
201
审稿时长
4-8 weeks
期刊介绍: Cell Host & Microbe is a scientific journal that was launched in March 2007. The journal aims to provide a platform for scientists to exchange ideas and concepts related to the study of microbes and their interaction with host organisms at a molecular, cellular, and immune level. It publishes novel findings on a wide range of microorganisms including bacteria, fungi, parasites, and viruses. The journal focuses on the interface between the microbe and its host, whether the host is a vertebrate, invertebrate, or plant, and whether the microbe is pathogenic, non-pathogenic, or commensal. The integrated study of microbes and their interactions with each other, their host, and the cellular environment they inhabit is a unifying theme of the journal. The published work in Cell Host & Microbe is expected to be of exceptional significance within its field and also of interest to researchers in other areas. In addition to primary research articles, the journal features expert analysis, commentary, and reviews on current topics of interest in the field.
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