Impact of streptozotocin-induced diabetes on experimental masseter pain in rats.

IF 1.5 4区 医学 Q3 DENTISTRY, ORAL SURGERY & MEDICINE Brazilian oral research Pub Date : 2024-08-05 eCollection Date: 2024-01-01 DOI:10.1590/1807-3107bor-2024.vol38.0073
Yuri Martins Costa, Clarissa Carolina Fernandes Herculiani, Flávia Fonseca Carvalho Soares, Michelle de Campos Soriani Azevedo, Paulo César Rodrigues Conti, Thiago José Dionísio, Gabriela de Moraes Oliveira, Flávio Augusto Cardoso de Faria, Carlos Ferreira Santos, Gustavo Pompermaier Garlet, Leonardo Rigoldi Bonjardim
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Abstract

This study aimed to assess the influence of streptozotocin (STZ)-induced diabetes on the nociceptive behavior evoked by the injection of hypertonic saline (HS) into the masseter muscle of rats. Forty male rats were equally divided into four groups: a) isotonic saline control, which received 0.9% isotonic saline (IS), (Ctrl-IS); b) hypertonic saline control, which received 5% HS (Ctrl-HS); c) STZ-induced diabetic, which received IS, (STZ-IS); d) STZ-induced diabetic, which received HS (STZ-HS). Experimental diabetes was induced by a single intraperitoneal injection of STZ at dose of 60 mg/kg dissolved in 0.1 M citrate buffer, and 100 μL of HS or IS were injected into the left masseter to measure the nociceptive behavior. Later on, muscle RNA was extracted to measure the relative expression of the following cytokines: cyclooxygenase-2 (COX-2), tumor necrosis factor (TNF-α), and interleukins (IL)-1β, -2, -6, and -10. One-way analysis of variance (ANOVA) was applied to the data (p < 0.050). We observed a main effect of group on the nociceptive response (ANOVA: F = 11.60, p < 0.001), where the Ctrl-HS group presented the highest response (p < 0.001). However, nociceptive response was similar among the Ctrl-IS, STZ-IS, and STZ-HS group (p > 0.050). In addition, the highest relative gene expression of TNF-α and IL-6 was found in the masseter of control rats following experimental muscle pain (p < 0.050). In conclusion, the loss of somatosensory function can be observed in deep orofacial tissues of STZ-induced diabetic rats.

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链脲佐菌素诱导的糖尿病对实验性大鼠咀嚼肌疼痛的影响
本研究旨在评估链脲佐菌素(STZ)诱导的糖尿病对大鼠咀嚼肌注射高渗盐水(HS)所诱发的痛觉行为的影响。将 40 只雄性大鼠平均分为四组:a)等渗盐水对照组,即注射 0.9% 等渗盐水(IS)的大鼠(Ctrl-IS);b)高渗盐水对照组,即注射 5% HS 的大鼠(Ctrl-HS);c)STZ 诱导的糖尿病大鼠,即注射 IS 的大鼠(STZ-IS);d)STZ 诱导的糖尿病大鼠,即注射 HS 的大鼠(STZ-HS)。实验用60 mg/kg剂量的STZ溶于0.1 M柠檬酸盐缓冲液中诱导糖尿病患者腹腔注射,然后向左侧咀嚼肌注射100 μL的HS或IS以测量痛觉行为。随后提取肌肉 RNA 以测量以下细胞因子的相对表达:环氧化酶-2(COX-2)、肿瘤坏死因子(TNF-α)、白细胞介素(IL)-1β、-2、-6 和 -10。对数据进行了单因素方差分析(ANOVA)(P < 0.050)。我们观察到组别对痛觉反应的主效应(方差分析:F = 11.60,p < 0.001),其中 Ctrl-HS 组的痛觉反应最高(p < 0.001)。然而,Ctrl-IS 组、STZ-IS 组和 STZ-HS 组的痛觉反应相似(p > 0.050)。此外,实验性肌肉疼痛后,对照组大鼠咀嚼肌中 TNF-α 和 IL-6 的相对基因表达量最高(p < 0.050)。总之,在 STZ 诱导的糖尿病大鼠的口面部深层组织中可以观察到体感功能的丧失。
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来源期刊
CiteScore
3.70
自引率
4.00%
发文量
107
审稿时长
12 weeks
期刊最新文献
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