Unlocking Biological Activity and Metabolomics Insights: Primary Screening of Cyanobacterial Biomass from a Tropical Reservoir

IF 3.6 4区环境科学与生态学 Q2 ENVIRONMENTAL SCIENCES Environmental Toxicology and Chemistry Pub Date : 2024-08-07 DOI:10.1002/etc.5962

Rhuana Valdetário Médice, Renan Silva Arruda, Jaewon Yoon, Ricardo Moreira Borges, Natália Pessoa Noyma, Miquel Lürling, Camila Manoel Crnkovic, Marcelo Manzi Marinho, Ernani Pinto

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Abstract

Cyanobacterial harmful algal blooms can pose risks to ecosystems and human health worldwide due to their capacity to produce natural toxins. The potential dangers associated with numerous metabolites produced by cyanobacteria remain unknown. Only select classes of cyanopeptides have been extensively studied with the aim of yielding substantial evidence regarding their toxicity, resulting in their inclusion in risk management and water quality regulations. Information about exposure concentrations, co-occurrence, and toxic impacts of several cyanopeptides remains largely unexplored. We used liquid chromatography–mass spectrometry (LC–MS)-based metabolomic methods associated with chemometric tools (NP Analyst and Data Fusion-based Discovery), as well as an acute toxicity essay, in an innovative approach to evaluate the association of spectral signatures and biological activity from natural cyanobacterial biomass collected in a eutrophic reservoir in southeastern Brazil. Four classes of cyanopeptides were revealed through metabolomics: microcystins, microginins, aeruginosins, and cyanopeptolins. The bioinformatics tools showed high bioactivity correlation scores for compounds of the cyanopeptolin class (0.54), in addition to microcystins (0.54–0.58). These results emphasize the pressing need for a comprehensive evaluation of the (eco)toxicological risks associated with different cyanopeptides, considering their potential for exposure. Our study also demonstrated that the combined use of LC–MS/MS-based metabolomics and chemometric techniques for ecotoxicological research can offer a time-efficient strategy for mapping compounds with potential toxicological risk. Environ Toxicol Chem 2024;43:2222–2231. © 2024 SETAC

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揭开生物活性和代谢组学的神秘面纱：对热带水库中的蓝藻生物质进行初步筛选。

由于蓝藻能产生天然毒素，蓝藻有害藻华会对全球生态系统和人类健康造成危害。蓝藻产生的多种代谢物的潜在危害仍不为人知。目前只对部分类别的蓝藻肽进行了广泛研究，旨在获得有关其毒性的实质性证据，从而将其纳入风险管理和水质法规中。有关几种蓝藻肽的暴露浓度、共存性和毒性影响的信息在很大程度上仍未得到探索。我们采用基于液相色谱-质谱（LC-MS）的代谢组学方法、化学计量学工具（NP Analyst 和基于数据融合的发现）以及急性毒性论文，以一种创新的方法评估了从巴西东南部富营养化水库中采集的天然蓝藻生物质的光谱特征与生物活性之间的关联。通过代谢组学研究发现了四类蓝藻肽：微囊藻毒素、微精藻毒素、气藻毒素和蓝藻肽。生物信息学工具显示，除微囊藻毒素（0.54-0.58）外，氰肽类化合物的生物活性相关性得分也很高（0.54）。这些结果表明，考虑到不同蓝藻肽的潜在暴露风险，迫切需要对其相关（生态）毒理学风险进行全面评估。我们的研究还表明，在生态毒理学研究中结合使用基于 LC-MS/MS 的代谢组学和化学计量学技术，可为绘制具有潜在毒理学风险的化合物图谱提供一种省时高效的策略。环境毒物化学 2024;00:1-10。© 2024 SETAC.

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来源期刊

Environmental Toxicology and Chemistry 环境科学-毒理学

CiteScore

7.40

自引率

9.80%

发文量

265

审稿时长

3.4 months

期刊介绍： The Society of Environmental Toxicology and Chemistry (SETAC) publishes two journals: Environmental Toxicology and Chemistry (ET&C) and Integrated Environmental Assessment and Management (IEAM). Environmental Toxicology and Chemistry is dedicated to furthering scientific knowledge and disseminating information on environmental toxicology and chemistry, including the application of these sciences to risk assessment.[...] Environmental Toxicology and Chemistry is interdisciplinary in scope and integrates the fields of environmental toxicology; environmental, analytical, and molecular chemistry; ecology; physiology; biochemistry; microbiology; genetics; genomics; environmental engineering; chemical, environmental, and biological modeling; epidemiology; and earth sciences. ET&C seeks to publish papers describing original experimental or theoretical work that significantly advances understanding in the area of environmental toxicology, environmental chemistry and hazard/risk assessment. Emphasis is given to papers that enhance capabilities for the prediction, measurement, and assessment of the fate and effects of chemicals in the environment, rather than simply providing additional data. The scientific impact of papers is judged in terms of the breadth and depth of the findings and the expected influence on existing or future scientific practice. Methodological papers must make clear not only how the work differs from existing practice, but the significance of these differences to the field. Site-based research or monitoring must have regional or global implications beyond the particular site, such as evaluating processes, mechanisms, or theory under a natural environmental setting.

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