Nitrogen sources in donor human milk: True protein, nonprotein nitrogen, and amino acid profile.

IF 2.1 4区 医学 Q3 NUTRITION & DIETETICS Nutrition in Clinical Practice Pub Date : 2024-08-06 DOI:10.1002/ncp.11199
Brian K Stansfield, Amy Gates
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Abstract

Background: Devices measuring the macronutrient content of human milk are commonly used to assist with clinical decision-making. It is unknown if these devices accurately measure protein content in donor human milk (DHM). Our objective is to quantify the nitrogen sources and protein content in commercial DHM.

Methods: The total nitrogen content (Dumas method) and nonprotein nitrogen content (Kjeldahl method) was measured in triplicate from six commercial DHM samples with protein content noted on the labels. In addition, the amino acid content was measured in 15 commercial DHM samples and protein content in each sample was calculated. The calculated protein content for each DHM sample was compared for consistency.

Results: The nonprotein nitrogen content in DHM was consistently higher (0.33 ± 0.05 g/g) than previous reports, leading to overreporting of protein content on DHM labels by a median value of 0.15 g/dl (range 0.02-0.23 g/dl). Similarly, calculation of the protein content from the total nitrogen content with an assumption of 20% (grams per gram) nonprotein nitrogen consistently overrepresented the protein content as determined from the amino acid profile for DHM.

Conclusion: Common methods for assessing the macronutrient content of human milk may overestimate the protein content of DHM.

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供体母乳中的氮源:真蛋白、非蛋白氮和氨基酸谱。
背景:测量母乳中宏量营养素含量的仪器通常用于协助临床决策。目前还不清楚这些设备是否能准确测量供体母乳(DHM)中的蛋白质含量。我们的目标是量化商业人乳中的氮源和蛋白质含量:方法:对六份标有蛋白质含量的商用 DHM 样品进行一式三份的总氮含量(杜马斯法)和非蛋白氮含量(凯氏定氮法)测定。此外,还测量了 15 个商用 DHM 样品的氨基酸含量,并计算出每个样品的蛋白质含量。对每个 DHM 样品计算出的蛋白质含量进行比较,以确保一致性:结果:DHM 中的非蛋白氮含量(0.33 ± 0.05 g/g)始终高于之前的报告,导致 DHM 标签上的蛋白质含量多报了 0.15 g/dl(范围为 0.02-0.23 g/dl)。同样,根据总氮含量计算蛋白质含量时,假设非蛋白氮含量为 20%(克/克),则根据 DHM 的氨基酸谱确定的蛋白质含量始终偏高:结论:评估母乳中宏量营养素含量的常用方法可能会高估 DHM 的蛋白质含量。
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来源期刊
CiteScore
6.00
自引率
9.70%
发文量
128
审稿时长
3 months
期刊介绍: NCP is a peer-reviewed, interdisciplinary publication that publishes articles about the scientific basis and clinical application of nutrition and nutrition support. NCP contains comprehensive reviews, clinical research, case observations, and other types of papers written by experts in the field of nutrition and health care practitioners involved in the delivery of specialized nutrition support. This journal is a member of the Committee on Publication Ethics (COPE).
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