The mechanisms of zinc-induced root growth inhibition in the zinc hyperaccumulator Noccaea caerulescens and the non-accumulator Microthlaspi perfoliatum
Natalia V. Zhukovskaya, Anna D. Kozhevnikova, Nina F. Lunkova, Tatiana Yu. Lykova, Alexander V. Kartashov, Victor B. Ivanov, Henk Schat, Ilya V. Seregin
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Abstract
Aims
This study aimed at revealing the mechanisms of zinc(Zn)-induced root growth inhibition in the Zn hyperaccumulator Noccaea caerulescens and the excluder Microthlaspi perfoliatum to shed light on the intriguing question whether there is any selectivity in the Zn effects on root cell division and elongation.
Methods
Zinc effects on various parameters characterizing root cell division and elongation were studied. Total Zn uptake, accumulation, root-to-shoot translocation, and distribution over the root tip tissues as well as metal-induced oxidative stress were also assessed.
Results
Similar degrees of root growth inhibition were achieved when the Zn concentration in the medium and Zn content in the roots were 150 and 5 times higher, respectively, in the hyperaccumulator, compared to the excluder. Zinc accumulated in the cell walls and protoplasts in the root meristem and elongation zone. Although Zn negatively affected both root cell division and elongation, at a similar degree of root growth inhibition, the contribution of the inhibition of cell division was greater in N. caerulescens compared to M. perfoliatum, as the decrease in the meristem length, the number of meristematic cells in a file, and the mitotic index were more prominent in the hyperaccumulator.
Conclusions
Greater contribution of the inhibition of cell division in N. caerulescens compared to M. perfoliatum at a similar degree of root growth inhibition seemed to be partly determined by a stronger degree of oxidative stress and disturbance of mineral nutrition in the roots of the hyperaccumulator, owing to a much higher Zn accumulation.
期刊介绍:
Plant and Soil publishes original papers and review articles exploring the interface of plant biology and soil sciences, and that enhance our mechanistic understanding of plant-soil interactions. We focus on the interface of plant biology and soil sciences, and seek those manuscripts with a strong mechanistic component which develop and test hypotheses aimed at understanding underlying mechanisms of plant-soil interactions. Manuscripts can include both fundamental and applied aspects of mineral nutrition, plant water relations, symbiotic and pathogenic plant-microbe interactions, root anatomy and morphology, soil biology, ecology, agrochemistry and agrophysics, as long as they are hypothesis-driven and enhance our mechanistic understanding. Articles including a major molecular or modelling component also fall within the scope of the journal. All contributions appear in the English language, with consistent spelling, using either American or British English.