The relationship between resistance evolution and carbon metabolism in Staphylococcus xylosus under ceftiofur sodium stress

IF 4.6 Q2 MATERIALS SCIENCE, BIOMATERIALS ACS Applied Bio Materials Pub Date : 2024-08-08 DOI:10.1007/s00203-024-04093-2

Qianwei Qu, Haixin Peng, Mo Chen, Xin Liu, Ruixiang Che, God’spower Bello-Onaghise, Zhiyun Zhang, Xueying Chen, Yanhua Li

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Abstract

Staphylococcus xylosus has emerged as a bovine mastitis pathogen with increasing drug resistance, resulting in substantial economic impacts. This study utilized iTRAQ analysis to investigate the mechanisms driving resistance evolution in S. xylosus under ceftiofur sodium stress. Findings revealed notable variations in the expression of 143 proteins, particularly glycolysis-related proteins (TpiA, Eno, GlpD, Ldh) and peptidoglycan (PG) hydrolase Atl. Following the induction of ceftiofur sodium resistance in S. xylosus, the emergence of resistant strains displaying characteristics of small colony variants (SCVs) was observed. The transcript levels of TpiA, Eno, GlpD and Ldh were up-regulated, TCA cycle proteins (ICDH, MDH) and Atl were down-regulated, lactate content was increased, and NADH concentration was decreased in SCV compared to the wild strain. That indicates a potential role of carbon metabolism, specifically PG hydrolysis, glycolysis, and the TCA cycle, in the development of resistance to ceftiofur sodium in S. xylosus.

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头孢噻呋钠胁迫下木葡萄球菌的抗性进化与碳代谢之间的关系

木葡萄球菌是一种牛乳腺炎病原体，其耐药性不断增加，造成了巨大的经济损失。本研究利用 iTRAQ 分析方法研究了头孢噻呋钠胁迫下木葡萄球菌耐药性进化的驱动机制。研究结果显示，143 种蛋白质的表达发生了显著变化，尤其是糖酵解相关蛋白质（TpiA、Eno、GlpD、Ldh）和肽聚糖（PG）水解酶 Atl。在诱导木虱对头孢噻呋钠产生耐药性后，观察到耐药菌株出现了小菌落变异株（SCVs）的特征。与野生菌株相比，SCV中TpiA、Eno、GlpD和Ldh的转录水平上调，TCA循环蛋白（ICDH、MDH）和Atl下调，乳酸含量增加，NADH浓度降低。这表明碳代谢，特别是 PG 水解、糖酵解和 TCA 循环在木虱对头孢噻呋钠产生抗性的过程中可能起了作用。

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