Comparative pharmacokinetic study of Anisodamine Hydrobromide tablets and injection in septic acute lung injury rats.

IF 1.9 4区医学 Q3 BIOCHEMICAL RESEARCH METHODS Bioanalysis Pub Date : 2024-08-08 DOI:10.1080/17576180.2024.2383106

Rui Wu, Fang Zhang, Yanfang Liu, Yujie Yu, Jianlan Zhang, Chenhao Yao, Shu Dai, Feng Wan, Feng Nan, Yunxia Li

引用次数: 0

Abstract

Aim: We aimed to establish a sensitive LC-MS/MS method to analyze the pharmacokinetics of Ani HBr tablets and injection. Methods: Around 10 mmNH₄Ac containing 0.1% formic acid and acetonitrile were used as the mobile phase. Acute lung injury in septic and normal rats, respectively, were administered Ani HBr tablets at doses of 12.5, 25 and 50 mg/kg and injection at doses of 4, 8 and 16 mg/kg, followed by extraction of the drugs from plasma using ethyl acetate for subsequent analysis. Results & conclusion: The method met the requirements for biological analysis. Ani HBr tablets absorbed slowly in rats with disease, tail vein administration was a more promising approach for treating septic acute lung injury.

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脓毒症急性肺损伤大鼠服用氢溴酸阿尼索达明片剂和注射剂的药代动力学比较研究

目的：我们旨在建立一种灵敏的 LC-MS/MS 方法来分析安利 HBr 片剂和注射剂的药代动力学。方法以含 0.1%甲酸的 10 mmNH4Ac 左右的乙腈为流动相。分别给急性肺损伤败血症大鼠和正常大鼠服用剂量为 12.5、25 和 50 mg/kg 的 Ani HBr 片剂和剂量为 4、8 和 16 mg/kg 的注射剂，然后用乙酸乙酯从血浆中提取药物进行后续分析。结果与结论该方法符合生物分析的要求。Ani HBr 片剂在患病大鼠体内吸收缓慢，尾静脉给药是治疗化脓性急性肺损伤的一种更有前景的方法。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Bioanalysis BIOCHEMICAL RESEARCH METHODS-CHEMISTRY, ANALYTICAL

CiteScore

3.30

自引率

16.70%

发文量

审稿时长

2 months

期刊介绍： Reliable data obtained from selective, sensitive and reproducible analysis of xenobiotics and biotics in biological samples is a fundamental and crucial part of every successful drug development program. The same principles can also apply to many other areas of research such as forensic science, toxicology and sports doping testing. The bioanalytical field incorporates sophisticated techniques linking sample preparation and advanced separations with MS and NMR detection systems, automation and robotics. Standards set by regulatory bodies regarding method development and validation increasingly define the boundaries between speed and quality. Bioanalysis is a progressive discipline for which the future holds many exciting opportunities to further reduce sample volumes, analysis cost and environmental impact, as well as to improve sensitivity, specificity, accuracy, efficiency, assay throughput, data quality, data handling and processing. The journal Bioanalysis focuses on the techniques and methods used for the detection or quantitative study of analytes in human or animal biological samples. Bioanalysis encourages the submission of articles describing forward-looking applications, including biosensors, microfluidics, miniaturized analytical devices, and new hyphenated and multi-dimensional techniques. Bioanalysis delivers essential information in concise, at-a-glance article formats. Key advances in the field are reported and analyzed by international experts, providing an authoritative but accessible forum for the modern bioanalyst.