Dopaminergic and anti-estrogenic responses in juvenile steelhead (Oncorhynchus mykiss) exposed to bifenthrin

IF 3.9 3区 环境科学与生态学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY Comparative Biochemistry and Physiology C-toxicology & Pharmacology Pub Date : 2024-08-05 DOI:10.1016/j.cbpc.2024.109995
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Abstract

The frequency of detection and concentrations of bifenthrin, a pyrethroid insecticide, in the waterways inhabited by the endangered species, steelhead trout (Oncorhynchus mykiss), has become a significant concern for regulatory agencies. Endocrine disruption has been observed with estrogenic and anti-estrogenic responses in fish species at different life stages. Since several studies have indicated alterations in dopaminergic signaling associated with endocrine responses, juvenile steelhead were exposed to environmentally relevant concentrations of 60 or 120 ng/L bifenthrin for two weeks. Fish brains were assessed for dopamine levels and the expression of genes involved in dopaminergic and estrogenic processes, such as catechol-o-methyltransferase (comt) and monoamine oxidase (mao). Vitellogenin (vtg) and estrogenic receptors (ERα1, ERβ1, and ERβ2) were also evaluated in livers of the animals. Dopamine concentrations were significantly higher in fish brains following bifenthrin exposure. Consistent with a reduction in dopamine clearance, there was a significant decrease in the mRNA expression of comt with increased bifenthrin concentration. Hepatic expression of ERα1 and ERβ2 mRNA was significantly decreased with increased bifenthrin concentration. These data support the possible mechanism of bifenthrin altering the dopaminergic pathway at low ng/L concentrations, in juvenile steelhead, which could interfere with endocrine feedback loops. These findings support the need for and importance of identifying species and life stage differences in pesticide modes of action to reduce uncertainties in risk assessments.

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暴露于联苯菊酯的钢鳟鱼幼鱼的多巴胺能和抗雌激素反应。
在濒危物种钢鳟鱼(Oncorhynchus mykiss)栖息的水道中,联苯菊酯(一种拟除虫菊酯杀虫剂)的检测频率和浓度已成为监管机构关注的一个重要问题。在不同生命阶段的鱼类物种中,已观察到雌激素和抗雌激素反应对内分泌的干扰。由于多项研究表明多巴胺能信号的改变与内分泌反应有关,因此将幼年钢鲤暴露于环境相关浓度为 60 或 120 纳克/升的联苯菊酯中两周。对鱼脑中的多巴胺水平以及参与多巴胺能和雌激素过程的基因(如儿茶酚-邻甲基转移酶(comt)和单胺氧化酶(mao))的表达进行了评估。还对动物肝脏中的卵黄素(vtg)和雌激素受体(ERα1、ERβ1 和 ERβ2)进行了评估。接触联苯菊酯后,鱼脑中的多巴胺浓度明显升高。与多巴胺清除率降低相一致,随着联苯菊酯浓度的增加,comt的mRNA表达量也显著下降。肝脏中 ERα1 和 ERβ2 mRNA 的表达量随着联苯菊酯浓度的增加而显著下降。这些数据支持联苯菊酯在低浓度(ng/L)时改变钢鲤幼鱼多巴胺能通路的可能机制,这可能会干扰内分泌反馈回路。这些发现支持了确定农药作用模式中物种和生命阶段差异的必要性和重要性,以减少风险评估中的不确定性。
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来源期刊
CiteScore
7.50
自引率
5.10%
发文量
206
审稿时长
30 days
期刊介绍: Part C: Toxicology and Pharmacology. This journal is concerned with chemical and drug action at different levels of organization, biotransformation of xenobiotics, mechanisms of toxicity, including reactive oxygen species and carcinogenesis, endocrine disruptors, natural products chemistry, and signal transduction with a molecular approach to these fields.
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