Effects of eczema calming lotion on the stratum corneum in atopic dermatitis: Corneodesmosin and intercellular lipid lamellae

IF 2.7 4区医学 Q2 DERMATOLOGY International Journal of Cosmetic Science Pub Date : 2024-08-07 DOI:10.1111/ics.12962

Hilal Ilarslan, William F. Lathrop, Brian Dobkowski, Stacy S. Hawkins, Jane Scott, John Bajor, Andrew E. Mayes

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Abstract

Objective

Atopic dermatitis (AD) is characterized by compositional and structural changes to the skin at lesional sites. Alteration to the levels and organization of both protein and lipid components are associated with disease status and lead to impaired barrier and hydration. Corneodesmosin (CDSN) and the arrangement and length of the intercellular lipid lamellae (ICLL) are altered in disrupted skin states. The aim of this research was to profile the distribution of CDSN and the ICLL in the stratum corneum (SC) at lesional and non-lesional sites in AD-prone skin and to investigate the impact of an eczema calming lotion containing petroleum jelly, fatty acids, and colloidal oatmeal.

Methods

An IRB-approved study was conducted with participants with active AD. From a small subset of participants, tape strips were collected from lesional and non-lesional sites on the arm, prior to and after twice daily application, over 4 weeks of an eczema calming lotion containing petroleum jelly, fatty acids, and colloidal oatmeal. Fluorescent antibody staining was used to investigate the distribution of CDSN. Transmission electron microscopy (TEM) was used to characterize the ICLL.

Results

The distribution/coverage of CDSN was similar between lesional and non-lesional sites at baseline; application of the lotion resulted in a more defined honeycomb/peripheral distribution. Normalized ICLL (nICLL) was lower in baseline samples from lesional sites relative to non-lesional sites. Application of the lotion increased this parameter by the end of the study at all sites.

Conclusion

The eczema calming lotion containing petroleum jelly, fatty acids and colloidal oatmeal provided changes in corneodesmosomal proteins distribution and ICLL, consistent with improvements in corneocyte maturation and improved barrier function in the skin of individuals with atopic dermatitis.

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湿疹镇静露对特应性皮炎患者角质层的影响：角质脱落蛋白和细胞间脂质层

目的：特应性皮炎（AD）的特点是病变部位皮肤的成分和结构发生变化。蛋白质和脂质成分水平和组织的改变与疾病状态有关，并导致屏障和水合功能受损。角质脱落蛋白（CDSN）和细胞间脂质层（ICLL）的排列和长度在皮肤受损状态下会发生改变。本研究的目的是分析 CDSN 和 ICLL 在 AD 易患皮肤病变和非病变部位的角质层（SC）中的分布情况，并调查含有凡士林、脂肪酸和胶体燕麦的湿疹镇静乳液的影响：方法：对患有活动性 AD 的参与者进行了一项经 IRB 批准的研究。从一小部分参与者中收集了手臂上皮损和非皮损部位的胶带条，在使用含有凡士林、脂肪酸和胶体燕麦的湿疹镇静乳液之前和之后，每天使用两次，连续使用 4 周。荧光抗体染色用于研究 CDSN 的分布。透射电子显微镜（TEM）用于分析 ICLL 的特征：结果：基线时，CDSN在病变部位和非病变部位的分布/覆盖率相似；涂抹乳液后，CDSN呈更清晰的蜂窝状/周边分布。在基线样本中，病变部位的归一化 ICLL（nICLL）低于非病变部位。到研究结束时，所有部位涂抹乳液后，这一参数都有所提高：结论：含有凡士林、脂肪酸和胶体燕麦的湿疹镇静乳液能改变角质层蛋白的分布和 ICLL，这与特应性皮炎患者的角质层细胞成熟和屏障功能改善是一致的。

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来源期刊

International Journal of Cosmetic Science DERMATOLOGY-

CiteScore

4.60

自引率

4.30%

发文量

期刊介绍： The Journal publishes original refereed papers, review papers and correspondence in the fields of cosmetic research. It is read by practising cosmetic scientists and dermatologists, as well as specialists in more diverse disciplines that are developing new products which contact the skin, hair, nails or mucous membranes. The aim of the Journal is to present current scientific research, both pure and applied, in: cosmetics, toiletries, perfumery and allied fields. Areas that are of particular interest include: studies in skin physiology and interactions with cosmetic ingredients, innovation in claim substantiation methods (in silico, in vitro, ex vivo, in vivo), human and in vitro safety testing of cosmetic ingredients and products, physical chemistry and technology of emulsion and dispersed systems, theory and application of surfactants, new developments in olfactive research, aerosol technology and selected aspects of analytical chemistry.