Combined transcriptome and metabolome analysis identifies triterpenoid-induced defense responses in Myzus persicae Sülzer-infested peach.

IF 5.6 2区 生物学 Q1 PLANT SCIENCES Journal of Experimental Botany Pub Date : 2024-10-30 DOI:10.1093/jxb/erae339
Lei Pan, Rui Huang, Zhenhua Lu, Wenyi Duan, Shihang Sun, Lele Yan, Guochao Cui, Liang Niu, Zhiqiang Wang, Wenfang Zeng
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Abstract

Piercing/sucking insects such as green peach aphid (GPA) (Myzus persicae) cause direct damage by obtaining phloem nutrients and indirect damage by spreading plant viruses. To investigate the response of peach trees (Prunus persica) to aphids, the leaf transcriptome and metabolome of two genotypes with different sensitivities to GPA were studied. The gene expression of aphid-susceptible plants infested with aphids was similar to that of control plants, whereas the gene expression of aphid-resistant plants infested with aphids showed strong induced changes in gene expression compared with control plants. Furthermore, gene transcripts in defense-related pathways, including plant-pathogen interaction, MAPK signaling, and several metabolic pathways, were strongly enriched upon aphid infestation. Untargeted secondary metabolite profiling confirmed that aphid infestation induced larger changes in aphid-resistant than in aphid-susceptible peaches. Consistent with transcriptomic alterations, nine triterpenoids showed highly significant GPA-induced accumulation in aphid-resistant peaches, whereas triterpenoid abundance remained predominantly unchanged or undetected in aphid-susceptible peaches. Furthermore, some types of transcription factors (including WRKYs, ERFs, and NACs) were strongly induced upon GPA infestation in aphid-resistant, but not in aphid-susceptible peaches. These results suggested that the accumulation of specialized triterpenoids and the corresponding pathway transcripts may play a key role in peach GPA resistance.

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结合转录组和代谢组分析,确定三萜类化合物诱导的受桃螨侵染的防御反应。
桃(Prunus persica)对昆虫攻击的防御反应包括基因表达和代谢物的变化。桃蚜等穿刺/吸食昆虫通过获取韧皮部养分造成直接损害,并通过传播植物病毒造成间接损害。为了研究桃树对蚜虫的反应,研究了两种对绿桃蚜(GPA,Myzus persicae)敏感性不同的基因型的叶片转录组和代谢组。受感染桃叶的转录组分析显示了两种不同的反应模式。对蚜虫敏感的桃植株受蚜虫感染后的基因表达与对照植株较为相似,而抗蚜虫的桃植株受蚜虫感染后的基因表达与对照植株相比有强烈的诱导变化。此外,防御相关通路(包括植物与病原体相互作用、MAPK 信号转导和几种代谢通路)中的基因转录本在蚜虫侵染时更为丰富。非靶向次生代谢物分析证实,与蚜虫易感性桃子相比,蚜虫处理在蚜虫抗性桃子中引起的变化更大。与转录组变化相一致的是,在抗蚜虫桃子中,有九种三萜类化合物在 GPA 诱导下出现了极其显著的积累,而在易受蚜虫影响的桃子中,三萜类化合物的丰度主要保持不变或未被检测到。此外,某些类型的转录因子(包括 WRKYs、ERFs、NACs 等)在抗蚜虫桃子受到 GPA 侵染时被更强烈地诱导,而在蚜虫易感桃子中则没有被诱导。蚜虫取食依赖性转录组和代谢物图谱为了解桃对蚜虫侵袭的响应分子机制奠定了基础。这些结果表明,特化三萜类化合物和相应途径转录本的积累可能在桃抗旱害病中起着关键作用。
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来源期刊
Journal of Experimental Botany
Journal of Experimental Botany 生物-植物科学
CiteScore
12.30
自引率
4.30%
发文量
450
审稿时长
1.9 months
期刊介绍: The Journal of Experimental Botany publishes high-quality primary research and review papers in the plant sciences. These papers cover a range of disciplines from molecular and cellular physiology and biochemistry through whole plant physiology to community physiology. Full-length primary papers should contribute to our understanding of how plants develop and function, and should provide new insights into biological processes. The journal will not publish purely descriptive papers or papers that report a well-known process in a species in which the process has not been identified previously. Articles should be concise and generally limited to 10 printed pages.
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