{"title":"Engineering modular enzymes using DNA origami","authors":"","doi":"10.1038/s41565-024-01739-6","DOIUrl":null,"url":null,"abstract":"A DNA origami nanocompartment is designed to trap an unfoldase machine in a unidirectional orientation. This trapping provides a gateway mechanism for substrate recruitment and translocation to a downstream compartment that hosts a protease. Kinetics and proteomics data demonstrate that the physical connection of the DNA-based modules improves the global performance of the chimera and reduces off-target reactions.","PeriodicalId":18915,"journal":{"name":"Nature nanotechnology","volume":"19 10","pages":"1440-1441"},"PeriodicalIF":38.1000,"publicationDate":"2024-08-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Nature nanotechnology","FirstCategoryId":"88","ListUrlMain":"https://www.nature.com/articles/s41565-024-01739-6","RegionNum":1,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"MATERIALS SCIENCE, MULTIDISCIPLINARY","Score":null,"Total":0}
引用次数: 0
Abstract
A DNA origami nanocompartment is designed to trap an unfoldase machine in a unidirectional orientation. This trapping provides a gateway mechanism for substrate recruitment and translocation to a downstream compartment that hosts a protease. Kinetics and proteomics data demonstrate that the physical connection of the DNA-based modules improves the global performance of the chimera and reduces off-target reactions.
期刊介绍：
Nature Nanotechnology is a prestigious journal that publishes high-quality papers in various areas of nanoscience and nanotechnology. The journal focuses on the design, characterization, and production of structures, devices, and systems that manipulate and control materials at atomic, molecular, and macromolecular scales. It encompasses both bottom-up and top-down approaches, as well as their combinations.
Furthermore, Nature Nanotechnology fosters the exchange of ideas among researchers from diverse disciplines such as chemistry, physics, material science, biomedical research, engineering, and more. It promotes collaboration at the forefront of this multidisciplinary field. The journal covers a wide range of topics, from fundamental research in physics, chemistry, and biology, including computational work and simulations, to the development of innovative devices and technologies for various industrial sectors such as information technology, medicine, manufacturing, high-performance materials, energy, and environmental technologies. It includes coverage of organic, inorganic, and hybrid materials.
京公网安备 11010802042870号
京ICP备2023020795号-1
分享
求助内容：
应助结果提醒方式：
扫码关注我们
