Microfluidic device: A versatile biosensor platform to multiplex aptamer-based detection of malaria biomarkers

IF 2.8 3区生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY Cell Biochemistry and Function Pub Date : 2024-08-08 DOI:10.1002/cbf.4104

Adewoyin M. Ogunmolasuyi, Mary A. Adewoyin

{"title":"Microfluidic device: A versatile biosensor platform to multiplex aptamer-based detection of malaria biomarkers","authors":"Adewoyin M. Ogunmolasuyi, Mary A. Adewoyin","doi":"10.1002/cbf.4104","DOIUrl":null,"url":null,"abstract":"Plasmodium falciparum malaria remains a dominant infectious disease that affects Africa than the rest of the world, considering its associated cases and death rates. It's a febrile illness that produces several reliable biomarkers, for example, P. falciparum lactate dehydrogenase (PfLDH), P. falciparum Plasmodium glutamate dehydrogenase (PfGDH), and P. falciparum histidine-rich proteins (HRP-II) in blood circulatory system that can easily be employed as targets in rapid diagnostic tests (RDTs). In recent times, several DNA aptamers have been developed via SELEX technology to detect some specific malaria biomarkers (PfLDH, PvLDH, HRP-II, PfGDH) in a biosensor mode with good binding affinity properties to overcome the trend of cross-reactivity, limited sensitivity and stability problems that have been observed with immunodiagnostics. In this review, we summarized existing diagnostic methods and relevant biomarkers to suggest promising approaches to develop sensitive and species-specific multiplexed diagnostic devices enabling effective detection of malaria in complex biological matrices and surveillance in the endemic region.","PeriodicalId":9669,"journal":{"name":"Cell Biochemistry and Function","volume":"42 6","pages":""},"PeriodicalIF":2.8000,"publicationDate":"2024-08-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Cell Biochemistry and Function","FirstCategoryId":"99","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1002/cbf.4104","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"BIOCHEMISTRY & MOLECULAR BIOLOGY","Score":null,"Total":0}

引用次数: 0

Abstract

Plasmodium falciparum malaria remains a dominant infectious disease that affects Africa than the rest of the world, considering its associated cases and death rates. It's a febrile illness that produces several reliable biomarkers, for example, P. falciparum lactate dehydrogenase (PfLDH), P. falciparum Plasmodium glutamate dehydrogenase (PfGDH), and P. falciparum histidine-rich proteins (HRP-II) in blood circulatory system that can easily be employed as targets in rapid diagnostic tests (RDTs). In recent times, several DNA aptamers have been developed via SELEX technology to detect some specific malaria biomarkers (PfLDH, PvLDH, HRP-II, PfGDH) in a biosensor mode with good binding affinity properties to overcome the trend of cross-reactivity, limited sensitivity and stability problems that have been observed with immunodiagnostics. In this review, we summarized existing diagnostic methods and relevant biomarkers to suggest promising approaches to develop sensitive and species-specific multiplexed diagnostic devices enabling effective detection of malaria in complex biological matrices and surveillance in the endemic region.

查看原文

微信好友朋友圈 QQ好友复制链接

本刊更多论文

微流控装置：多功能生物传感器平台，可基于多通道适配体检测疟疾生物标记物。

恶性疟原虫疟疾仍然是影响非洲的主要传染病，其发病率和死亡率高于世界其他地区。恶性疟原虫疟疾是一种发热性疾病，会在血液循环系统中产生多种可靠的生物标志物，例如恶性疟原虫乳酸脱氢酶（PfLDH）、恶性疟原虫谷氨酸脱氢酶（PfGDH）和恶性疟原虫富组氨酸蛋白（HRP-II），这些标志物很容易被用作快速诊断检测（RDT）的靶标。近来，人们通过 SELEX 技术开发了几种 DNA 嵌合体，以生物传感器模式检测一些特定的疟疾生物标志物（PfLDH、PvLDH、HRP-II、PfGDH），这些嵌合体具有良好的结合亲和性，克服了免疫诊断技术中存在的交叉反应趋势、灵敏度有限和稳定性问题。在这篇综述中，我们总结了现有的诊断方法和相关的生物标记物，提出了开发灵敏的、物种特异的多重诊断设备的可行方法，以便在复杂的生物基质中有效检测疟疾，并在疟疾流行地区进行监测。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文去求助

来源期刊

Cell Biochemistry and Function 生物-生化与分子生物学

CiteScore

6.20

自引率

0.00%

发文量

审稿时长

6-12 weeks

期刊介绍： Cell Biochemistry and Function publishes original research articles and reviews on the mechanisms whereby molecular and biochemical processes control cellular activity with a particular emphasis on the integration of molecular and cell biology, biochemistry and physiology in the regulation of tissue function in health and disease. The primary remit of the journal is on mammalian biology both in vivo and in vitro but studies of cells in situ are especially encouraged. Observational and pathological studies will be considered providing they include a rational discussion of the possible molecular and biochemical mechanisms behind them and the immediate impact of these observations to our understanding of mammalian biology.