Genomic characterization of carbapenemase-producing Enterobacterales from Dhaka food markets unveils the spread of high-risk antimicrobial-resistant clones and plasmids co-carrying bla NDM and mcr-1.1.

IF 3.7 Q2 INFECTIOUS DISEASES JAC-Antimicrobial Resistance Pub Date : 2024-08-08 eCollection Date: 2024-08-01 DOI:10.1093/jacamr/dlae124
Tania Tabassum Nisa, Yo Sugawara, Shigeto Hamaguchi, Dan Takeuchi, Ryuichiro Abe, Eisuke Kuroda, Masatomo Morita, Hui Zuo, Akiko Ueda, Isao Nishi, Nowrin Hossain, Md Mahmudul Hasan, Mahbubul H Siddiqee, Daisaku Nakatani, Ken Nakata, Yukihiro Akeda
{"title":"Genomic characterization of carbapenemase-producing Enterobacterales from Dhaka food markets unveils the spread of high-risk antimicrobial-resistant clones and plasmids co-carrying <i>bla</i> <sub>NDM</sub> and <i>mcr-1.1</i>.","authors":"Tania Tabassum Nisa, Yo Sugawara, Shigeto Hamaguchi, Dan Takeuchi, Ryuichiro Abe, Eisuke Kuroda, Masatomo Morita, Hui Zuo, Akiko Ueda, Isao Nishi, Nowrin Hossain, Md Mahmudul Hasan, Mahbubul H Siddiqee, Daisaku Nakatani, Ken Nakata, Yukihiro Akeda","doi":"10.1093/jacamr/dlae124","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong>The transmission of carbapenemase-producing Enterobacterales (CPE) in the external environment, especially through food, presents a significant public health risk.</p><p><strong>Objectives: </strong>To investigate the prevalence and genetic characteristics of CPE in food markets of Dhaka, Bangladesh, using WGS.</p><p><strong>Methods: </strong>CPE isolates were obtained from different food and water samples collected from food markets in the southern part of Dhaka, Bangladesh. The isolates subsequently underwent molecular typing, WGS employing both short- and long-read sequencers, and plasmid analysis.</p><p><strong>Results: </strong>This study unveiled an extensive spread of CPE, with no significant difference in contamination rates observed in samples (<i>N</i> = 136), including meat (<i>n</i> = 8), fish (<i>n</i> = 5), vegetables (<i>n</i> = 36) or various food-washed water (<i>n</i> = 65) from markets near hospitals or residential areas. Thirty-eight Enterobacterales from 33 samples carried carbapenemase genes (<i>bla</i> <sub>NDM-1, -4, -7</sub>, <i>bla</i> <sub>KPC-2</sub>, <i>bla</i> <sub>OXA-181</sub> or <i>bla</i> <sub>IMI-1</sub>). Among these, the high-risk <i>Escherichia coli</i> ST410 clone was the most prevalent and distributed across various locations. Furthermore, the identification of IncHI2 plasmids co-harbouring resistance genes like <i>bla</i> <sub>NDM-5</sub> and <i>mcr-1.1</i>, without discernible epidemiological connections, is a unique finding, suggesting their widespread dissemination.</p><p><strong>Conclusions: </strong>The analysis unveils a dynamic landscape of CPE dissemination in food markets, underscored by the proliferation of novel IncHI2 hybrid plasmids carrying both colistin- and carbapenem-resistance genes. This illuminates the ever-evolving landscape of antimicrobial resistance in Dhaka, urging us to confront its emergent challenges.</p>","PeriodicalId":14594,"journal":{"name":"JAC-Antimicrobial Resistance","volume":"6 4","pages":"dlae124"},"PeriodicalIF":3.7000,"publicationDate":"2024-08-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11306930/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"JAC-Antimicrobial Resistance","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1093/jacamr/dlae124","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2024/8/1 0:00:00","PubModel":"eCollection","JCR":"Q2","JCRName":"INFECTIOUS DISEASES","Score":null,"Total":0}
引用次数: 0

Abstract

Background: The transmission of carbapenemase-producing Enterobacterales (CPE) in the external environment, especially through food, presents a significant public health risk.

Objectives: To investigate the prevalence and genetic characteristics of CPE in food markets of Dhaka, Bangladesh, using WGS.

Methods: CPE isolates were obtained from different food and water samples collected from food markets in the southern part of Dhaka, Bangladesh. The isolates subsequently underwent molecular typing, WGS employing both short- and long-read sequencers, and plasmid analysis.

Results: This study unveiled an extensive spread of CPE, with no significant difference in contamination rates observed in samples (N = 136), including meat (n = 8), fish (n = 5), vegetables (n = 36) or various food-washed water (n = 65) from markets near hospitals or residential areas. Thirty-eight Enterobacterales from 33 samples carried carbapenemase genes (bla NDM-1, -4, -7, bla KPC-2, bla OXA-181 or bla IMI-1). Among these, the high-risk Escherichia coli ST410 clone was the most prevalent and distributed across various locations. Furthermore, the identification of IncHI2 plasmids co-harbouring resistance genes like bla NDM-5 and mcr-1.1, without discernible epidemiological connections, is a unique finding, suggesting their widespread dissemination.

Conclusions: The analysis unveils a dynamic landscape of CPE dissemination in food markets, underscored by the proliferation of novel IncHI2 hybrid plasmids carrying both colistin- and carbapenem-resistance genes. This illuminates the ever-evolving landscape of antimicrobial resistance in Dhaka, urging us to confront its emergent challenges.

查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
达卡食品市场中产碳青霉烯酶肠杆菌的基因组特征揭示了高风险抗菌克隆和共同携带 bla NDM 和 mcr-1.1 的质粒的扩散。
背景:产碳青霉烯酶肠杆菌(CPE)在外部环境中的传播,尤其是通过食物的传播,给公共卫生带来了巨大风险:使用 WGS 调查孟加拉国达卡食品市场中 CPE 的流行情况和遗传特征:从孟加拉国达卡南部食品市场采集的不同食物和水样中获得 CPE 分离物。这些分离物随后进行了分子分型、使用短线程和长线程测序仪的 WGS 以及质粒分析:这项研究揭示了 CPE 的广泛传播,在来自医院或居民区附近市场的肉类(8 个)、鱼类(5 个)、蔬菜(36 个)或各种食品洗涤水(65 个)等样本(136 个)中观察到的污染率没有明显差异。33 个样本中的 38 个肠杆菌携带碳青霉烯酶基因(bla NDM-1、-4、-7、bla KPC-2、bla OXA-181 或 bla IMI-1)。其中,高风险大肠埃希菌 ST410 克隆最为普遍,且分布在不同地点。此外,IncHI2 质粒与 bla NDM-5 和 mcr-1.1 等抗性基因共存,但没有明显的流行病学联系,这是一个独特的发现,表明这些基因广泛传播:分析揭示了 CPE 在食品市场上的动态传播情况,同时携带可乐定和碳青霉烯耐药基因的新型 IncHI2 杂交质粒的扩散更凸显了这一点。这揭示了达卡不断演变的抗菌药耐药性状况,敦促我们正视新出现的挑战。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 去求助
来源期刊
CiteScore
5.30
自引率
0.00%
发文量
0
审稿时长
16 weeks
期刊最新文献
Investigation of cefiderocol resistance prevalence and resistance mechanisms in carbapenem-resistant Pseudomonas aeruginosa, Germany 2019-21. Medical school ranking and provider outpatient Medicare Part D claims for antibiotics among older patients in the USA. Nursing home nurses' opinion profiles on the potential evolution of their role in antimicrobial stewardship and associated factors: a national cross-sectional study in France. Clinical pharmacist-led antifungal drug utilization reviews in cancer care hospital: a prospective audit and feedback. State of the art of real-life concentration monitoring of rifampicin and its implementation contextualized in resource-limited settings: the Tanzanian case.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1