Investigation of Tissue-Specific Distribution and Genetic Variation of Alfalfa Mosaic Virus and Chinese Artichoke Mosaic Virus in Chinese Artichoke (Stachys affinis miq.).

IF 1.8 3区 农林科学 Q2 PLANT SCIENCES Plant Pathology Journal Pub Date : 2024-08-01 DOI:10.5423/PPJ.OA.06.2024.0094
Ji-Soo Park, Dong-Joo Min, Tae-Seon Park, You-Seop Shin, Jin-Sung Hong
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Abstract

The Chinese artichoke (Stachys affinis syn. S. sieboldii) is a widely cultivated crop, and its rhizome is used as a medicinal vegetable. To investigate the causes of viral diseases in Chinese artichokes, the infection rates of four virus species infecting Chinese artichoke were investigated. Since the Chinese artichoke propagates through its tuber, this study aimed to determine whether viral transmission to the progeny is possible through the tuber, by identifying the virus present in the tuber and investigating its accumulation. First, reverse transcription polymerase chain reaction analysis was performed to detect viruses using total RNA extracted from the flowers, leaves, and tubers of Chinese artichoke plants. Alfalfa mosaic virus (AMV) and Chinese artichoke mosaic virus (ChAMV) had high infectivity in Chinese artichoke and most plants were simultaneously infected with AMV and ChAMV. These viruses were present in all tissues, but their detection frequency and accumulation rates varied across different tissues of the Chinese artichoke. Also, we sequenced the coat protein (CP) genes of AMV and ChAMV to investigate genetic variations of virus between the leaf and tuber. It provides information on CP gene sequences and genetic diversity of isolates identified from new hosts of AMV and ChAMV. This study offers valuable insights into the distribution and spread of the ChAMV and AMV within Chinese artichoke plants, which have implications for the management and control of viral infections in crops.

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紫花苜蓿花叶病毒和中国朝鲜蓟花叶病毒在中国朝鲜蓟(Stachys affinis miq.)
中国朝鲜蓟(Stachys affinis syn. S. sieboldii)是一种广泛种植的作物,其根茎可作为药用蔬菜。为了研究中国朝鲜蓟病毒病的成因,我们调查了感染中国朝鲜蓟的四种病毒的感染率。由于中国朝鲜蓟通过块茎繁殖,本研究旨在通过鉴定块茎中的病毒并调查其积累情况,确定病毒是否可能通过块茎传播给后代。首先,利用从中国朝鲜蓟植株的花、叶和块茎中提取的总 RNA 进行反转录聚合酶链反应分析,以检测病毒。紫花苜蓿花叶病毒(AMV)和中国朝鲜蓟花叶病毒(ChAMV)对中国朝鲜蓟的感染率很高,大多数植株同时感染了AMV和ChAMV。这些病毒存在于所有组织中,但在中国朝鲜蓟不同组织中的检测频率和累积率有所不同。此外,我们还对 AMV 和 ChAMV 的衣壳蛋白(CP)基因进行了测序,以研究病毒在叶片和块茎之间的遗传变异。该研究提供了有关 CP 基因序列以及从 AMV 和 ChAMV 的新宿主中鉴定出的分离株的遗传多样性的信息。这项研究为了解 ChAMV 和 AMV 在中国朝鲜蓟植株中的分布和传播提供了宝贵的信息,对管理和控制农作物病毒感染具有重要意义。
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来源期刊
Plant Pathology Journal
Plant Pathology Journal 生物-植物科学
CiteScore
4.90
自引率
4.30%
发文量
71
审稿时长
12 months
期刊介绍: Information not localized
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