Potentiality of Beneficial Microbe Bacillus siamensis GP-P8 for the Suppression of Anthracnose Pathogens and Pepper Plant Growth Promotion.

IF 1.8 3区 农林科学 Q2 PLANT SCIENCES Plant Pathology Journal Pub Date : 2024-08-01 DOI:10.5423/PPJ.OA.01.2024.0022
Ji Min Woo, Hyun Seung Kim, In Kyu Lee, Eun Jeong Byeon, Won Jun Chang, Youn Su Lee
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Abstract

This study was carried out to screen the antifungal activity against Colletotrichum acutatum, Colletotrichum dematium, and Colletotrichum coccodes. Bacterial isolate GP-P8 from pepper soil was found to be effective against the tested pathogens with an average inhibition rate of 70.7% in in vitro dual culture assays. 16S rRNA gene sequencing analysis result showed that the effective bacterial isolate as Bacillus siamensis. Biochemical characterization of GP-P8 was also performed. According to the results, protease and cellulose, siderophore production, phosphate solubilization, starch hydrolysis, and indole-3-acetic acid production were shown by the GP-P8. Using specific primers, genes involved in the production of antibiotics, such as iturin, fengycin, difficidin, bacilysin, bacillibactin, surfactin, macrolactin, and bacillaene were also detected in B. siamensis GP-P8. Identification and analysis of volatile organic compounds through solid phase microextraction/gas chromatography-mass spectrometry (SPME/GC-MS) revealed that acetoin and 2,3-butanediol were produced by isolate GP-P8. In vivo tests showed that GP-P8 significantly reduced the anthracnose disease caused by C. acutatum, and enhanced the growth of pepper plant. Reverse transcription polymerase chain reaction analysis of pepper fruits revealed that GP-P8 treated pepper plants showed increased expression of immune genes such as CaPR1, CaPR4, CaNPR1, CaMAPK4, CaJA2, and CaERF53. These results strongly suggest that GP-P8 could be a promising biocontrol agent against pepper anthracnose disease and possibly a pepper plant growth-promoting agent.

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有益微生物暹罗芽孢杆菌 GP-P8 抑制炭疽病病原体和促进辣椒植株生长的潜力。
本研究旨在筛选出对尖孢褐斑病菌(Colletotrichum acutatum)、褐斑病菌(Colletotrichum dematium)和球孢褐斑病菌(Colletotrichum coccodes)的抗真菌活性。在体外双重培养试验中发现,从辣椒土壤中分离出的细菌 GP-P8 对测试病原体有效,平均抑制率为 70.7%。16S rRNA 基因测序分析结果显示,有效的细菌分离物为暹罗芽孢杆菌。此外,还对 GP-P8 进行了生化鉴定。结果显示,GP-P8 能产生蛋白酶和纤维素、嗜苷酸、磷酸盐溶解、淀粉水解和吲哚-3-乙酸。使用特定引物,还在暹罗芽孢杆菌 GP-P8 中检测到了参与生产抗生素的基因,如 iturin、fengycin、difficidin、bacilysin、bacillibactin、surfactin、macrolactin 和 bacillaene。通过固相微萃取/气相色谱-质谱(SPME/GC-MS)对挥发性有机化合物的鉴定和分析表明,GP-P8 分离物产生了乙酰丙酮和 2,3-丁二醇。体内试验表明,GP-P8 能显著减轻由 C. acutatum 引起的炭疽病,并促进辣椒植株的生长。对辣椒果实进行的逆转录聚合酶链反应分析表明,GP-P8 处理过的辣椒植株的免疫基因(如 CaPR1、CaPR4、CaNPR1、CaMAPK4、CaJA2 和 CaERF53)的表达量有所增加。这些结果有力地表明,GP-P8 可能是一种很有前景的防治辣椒炭疽病的生物防治剂,也可能是一种促进辣椒植株生长的药剂。
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来源期刊
Plant Pathology Journal
Plant Pathology Journal 生物-植物科学
CiteScore
4.90
自引率
4.30%
发文量
71
审稿时长
12 months
期刊介绍: Information not localized
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