Storage of equine faecal microbiota transplantation solution has minimal impact on major bacterial communities and structure

IF 2.3 2区 农林科学 Q1 VETERINARY SCIENCES Veterinary journal Pub Date : 2024-08-06 DOI:10.1016/j.tvjl.2024.106220
J. Bell, S. Raidal, A. Peters, K.J. Hughes
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Abstract

Management of diarrhoea in horses is usually non-specific and supportive. Faecal microbiota transplantations (FMT) are used to manage dysbiosis in horses with diarrhoea. There are few studies investigating the effects of storage on prepared FMT solutions. This study was an in vitro non-randomised controlled experiment that investigated the effects of FMT solution preparation and storage on the faecal microbiota. Fresh faeces were collected from five healthy adult horses and used for DNA extraction and preparation of FMT. From each FMT, seven aliquots were collected and DNA was extracted immediately after FMT preparation (0 hr), after storage at 4 °C for 24, 48 or 72 hours, and after storage at −20°C for 7 days, 14 days or 28 days. The extracted DNA was used for 16 S rRNA gene sequencing.

The relative abundance, alpha diversity and beta diversity between fresh faeces and FMT 0 hr showed no differences (P ≥ 0.05). There were minimal changes in the microbiota of FMT stored at 4°C for up to 72 hours and −20°C for up to 28 days. The results of this study indicate that preparation of equine FMT solution has minimal effect on the microbiota in comparison to fresh faeces. FMT solution can be stored at 4°C for up to 3 days and −20°C for 28 days without major change in microbiota.

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马粪微生物群移植溶液的储存对主要细菌群落和结构的影响极小。
对马腹泻的治疗通常是非特异性和支持性的。粪便微生物群移植(FMT)可用于治疗腹泻马匹的菌群失调。很少有研究调查贮存对配制好的粪便微生物群移植溶液的影响。本研究是一项体外非随机对照实验,旨在调查 FMT 溶液配制和储存对粪便微生物群的影响。从五匹健康成年马身上收集新鲜粪便,用于提取 DNA 和制备 FMT。从每份 FMT 中收集 7 份等分样品,在配制 FMT 后(0 小时)、在 4oC 下储存 24、48 或 72 小时以及在 -20°C 下储存 7、14 或 28 天后立即提取 DNA。提取的 DNA 用于 16S rRNA 基因测序。新鲜粪便和 FMT 0hr 的相对丰度、α多样性和β多样性没有差异(P ≥ 0.05)。在 4°C 下储存 72 小时和在 -20°C 下储存 28 天的 FMT 微生物群变化极小。本研究结果表明,与新鲜粪便相比,配制马 FMT 溶液对微生物区系的影响极小。FMT 溶液可在 4°C 下储存 3 天,在 -20°C 下储存 28 天,微生物群不会发生重大变化。
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来源期刊
Veterinary journal
Veterinary journal 农林科学-兽医学
CiteScore
4.10
自引率
4.50%
发文量
79
审稿时长
40 days
期刊介绍: The Veterinary Journal (established 1875) publishes worldwide contributions on all aspects of veterinary science and its related subjects. It provides regular book reviews and a short communications section. The journal regularly commissions topical reviews and commentaries on features of major importance. Research areas include infectious diseases, applied biochemistry, parasitology, endocrinology, microbiology, immunology, pathology, pharmacology, physiology, molecular biology, immunogenetics, surgery, ophthalmology, dermatology and oncology.
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