Veterinary journal最新文献

Exploring the genetic influences on equine analgesic efficacy through genome-wide association analysis of ranked pain responses

IF 2.3 2区农林科学 Q1 VETERINARY SCIENCES

Veterinary journal

Pub Date : 2025-04-10 DOI: 10.1016/j.tvjl.2025.106347

Elouise K. Bacon , Callum G. Donnelly , Carrie J. Finno , Bianca Haase , Brandon D. Velie

Multimodal analgesic administration is a promising strategy for mitigating side effects typically associated with analgesia; nevertheless, variation in analgesic effectiveness still poses a considerable safety concern for both horses and veterinarians. Pharmacogenomic studies have started delving into genetic influences on varying drug effectiveness and related side effects. However, current findings have narrow implications and are limited in their ability to individualize analgesic dosages in horses. Hydromorphone and detomidine were administered to a cohort of 48 horses at standardized time intervals, with dosage rates recorded. Analgesic effectiveness was scored (1−3) based on pain response to dura penetration during cerebrospinal fluid centesis. Genome-wide association (GWA) analyses identified two SNVs passing the nominal significance threshold (P < 1 ×10⁻⁵) in association with analgesic effectiveness. One SNV identified on chromosome 27 (rs1142378599) is contained within the LOC100630731 disintegrin and metalloproteinase domain-containing protein 5 gene. The second identified SNV is an intergenic variant located on chromosome 29 (rs3430772468) These SNVs accounted for 26.11 % and 31.72 % of explained variation in analgesic effectiveness respectively, with all eight of the horses with the lowest analgesic effectiveness expressing the A/C genotype at rs3430772468, with six of which also expressing the C/T genotype at rs1142872965. Whilst highlighting the multifactorial nature of analgesic efficacy, this study serves as an important step in the application of genome-wide approaches to better understand genetic factors underpinning commonly observed variation in analgesic effectiveness in horses, with the goal of tailoring analgesic dosage to minimize commonly observed side effects and improve the outcomes of equine pain management.

{"title":"Exploring the genetic influences on equine analgesic efficacy through genome-wide association analysis of ranked pain responses","authors":"Elouise K. Bacon , Callum G. Donnelly , Carrie J. Finno , Bianca Haase , Brandon D. Velie","doi":"10.1016/j.tvjl.2025.106347","DOIUrl":"10.1016/j.tvjl.2025.106347","url":null,"abstract":"<div><div>Multimodal analgesic administration is a promising strategy for mitigating side effects typically associated with analgesia; nevertheless, variation in analgesic effectiveness still poses a considerable safety concern for both horses and veterinarians. Pharmacogenomic studies have started delving into genetic influences on varying drug effectiveness and related side effects. However, current findings have narrow implications and are limited in their ability to individualize analgesic dosages in horses. Hydromorphone and detomidine were administered to a cohort of 48 horses at standardized time intervals, with dosage rates recorded. Analgesic effectiveness was scored (1−3) based on pain response to dura penetration during cerebrospinal fluid centesis. Genome-wide association (GWA) analyses identified two SNVs passing the nominal significance threshold (P < 1 ×10<sup>−5</sup>) in association with analgesic effectiveness. One SNV identified on chromosome 27 (rs1142378599) is contained within the <em>LOC100630731 disintegrin and metalloproteinase domain-containing protein 5</em> gene. The second identified SNV is an intergenic variant located on chromosome 29 (rs3430772468) These SNVs accounted for 26.11 % and 31.72 % of explained variation in analgesic effectiveness respectively, with all eight of the horses with the lowest analgesic effectiveness expressing the A/C genotype at rs3430772468, with six of which also expressing the C/T genotype at rs1142872965. Whilst highlighting the multifactorial nature of analgesic efficacy, this study serves as an important step in the application of genome-wide approaches to better understand genetic factors underpinning commonly observed variation in analgesic effectiveness in horses, with the goal of tailoring analgesic dosage to minimize commonly observed side effects and improve the outcomes of equine pain management.</div></div>","PeriodicalId":23505,"journal":{"name":"Veterinary journal","volume":"312 ","pages":"Article 106347"},"PeriodicalIF":2.3,"publicationDate":"2025-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143820874","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Isolation method and characterization of adipocytes as a tool for equine obesity research – In vitro study

IF 2.3 2区农林科学 Q1 VETERINARY SCIENCES

Veterinary journal

Pub Date : 2025-04-07 DOI: 10.1016/j.tvjl.2025.106354

Paweł Antoni Kołodziejski , Natalia Leciejewska , Maciej Sassek , Leszek Nogowski , Małgorzata Szumacher-Strabel , Robert Mikuła , Maciej Gogulski , Ewa Pruszyńska-Oszmałek

Adipose tissue functions as an endocrine organ; however, excessive lipid accumulation can lead to obesity and metabolic disorders, such as Equine Metabolic Syndrome (EMS), characterized by insulin resistance, fat deposition, and increased inflammation. Despite the growing prevalence of obesity in horses, knowledge of equine adipocytes and their metabolic functions remains limited. The main objective of the study was to develop and optimize a method for isolating equine adipocytes and to characterize their metabolic activity. Using slaughterhouse-derived horse visceral adipose tissue, we developed a protocol to isolate mature adipocytes. Metabolic activity of cells was assessed by examining their sensitivity to lipolytic factors: isoproterenol (0.001–10 µM), epinephrine (0.001–1 µM), and forskolin (0.001–1 µM)—and lipogenesis intensity after stimulation with insulin. We obtained mature equine adipocytes with diameters ranging from 50 to 160 µm. These cells demonstrated full metabolic functionality, responding to lipolytic factors such as isoproterenol (all doses: p < 0.001), epinephrine (0.01 µM: p < 0.05; 0.1–1 µM: p < 0.0001), and forskolin (0.001 µM: p < 0.0001). The adipocytes also responded to insulin from all tested species, with effects being dose- and time-dependent (after 2 h human insulin 10 nM, p < 0.05; bovine 10, 100 nM p < 0.05 and after 8 h all doses p < 0.05). The presented method for isolating mature equine adipocytes is effective, yielding metabolically functional cells, which can serve as a valuable in vitro model for studying the effects of various factors on adipocyte function, contributing to a better understanding of equine adipose tissue dysfunction, particularly in the context of metabolic disorders.

{"title":"Isolation method and characterization of adipocytes as a tool for equine obesity research – In vitro study","authors":"Paweł Antoni Kołodziejski , Natalia Leciejewska , Maciej Sassek , Leszek Nogowski , Małgorzata Szumacher-Strabel , Robert Mikuła , Maciej Gogulski , Ewa Pruszyńska-Oszmałek","doi":"10.1016/j.tvjl.2025.106354","DOIUrl":"10.1016/j.tvjl.2025.106354","url":null,"abstract":"<div><div>Adipose tissue functions as an endocrine organ; however, excessive lipid accumulation can lead to obesity and metabolic disorders, such as Equine Metabolic Syndrome (EMS), characterized by insulin resistance, fat deposition, and increased inflammation. Despite the growing prevalence of obesity in horses, knowledge of equine adipocytes and their metabolic functions remains limited. The main objective of the study was to develop and optimize a method for isolating equine adipocytes and to characterize their metabolic activity. Using slaughterhouse-derived horse visceral adipose tissue, we developed a protocol to isolate mature adipocytes. Metabolic activity of cells was assessed by examining their sensitivity to lipolytic factors: isoproterenol (0.001–10 µM), epinephrine (0.001–1 µM), and forskolin (0.001–1 µM)—and lipogenesis intensity after stimulation with insulin. We obtained mature equine adipocytes with diameters ranging from 50 to 160 µm. These cells demonstrated full metabolic functionality, responding to lipolytic factors such as isoproterenol (all doses: p < 0.001), epinephrine (0.01 µM: p < 0.05; 0.1–1 µM: p < 0.0001), and forskolin (0.001 µM: p < 0.0001). The adipocytes also responded to insulin from all tested species, with effects being dose- and time-dependent (after 2 h human insulin 10 nM, p < 0.05; bovine 10, 100 nM p < 0.05 and after 8 h all doses p < 0.05). The presented method for isolating mature equine adipocytes is effective, yielding metabolically functional cells, which can serve as a valuable in vitro model for studying the effects of various factors on adipocyte function, contributing to a better understanding of equine adipose tissue dysfunction, particularly in the context of metabolic disorders.</div></div>","PeriodicalId":23505,"journal":{"name":"Veterinary journal","volume":"312 ","pages":"Article 106354"},"PeriodicalIF":2.3,"publicationDate":"2025-04-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143815793","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluating the performance of VP1 expressed in baculovirus and Escherichia coli expressed from Senecavirus A in pig using an ELISA

IF 2.3 2区农林科学 Q1 VETERINARY SCIENCES

Veterinary journal

Pub Date : 2025-04-03 DOI: 10.1016/j.tvjl.2025.106346

Parin Watcharavongtip , Patumporn Jermsutjarit , Angkana Tantituvanont , Dachrit Nilubol

Senecavirus A (SVA) causes porcine idiopathic vesicular disease (PIVD), leading to economic losses in swine production. Rapid antibody detection is essential for monitoring herd immunity and controlling outbreaks. This study developed indirect ELISAs using SVA VP1 protein expressed in baculovirus and Escherichia coli (E. coli) systems. The optimized ELISAs showed high sensitivity (baculovirus: 100 %, E. coli: 96.67 %) and specificity (both 96.67 %) with a cut-off of 0.40. Both correlated strongly with virus neutralization assays (VNA) and showed no cross-reactivity with other porcine pathogens. The agreement with VNA was strong (κ = 0.839) for the baculovirus-based ELISA and moderate (κ = 0.731) for the E. coli-based ELISA. These findings suggest that SVA VP1 protein from both expression systems can serve as an alternative for ELISA-based serological diagnosis of SVA, aiding in herd immunity assessment and disease control.

引用次数: 0

ChangQing compound relieves Eimeria tenella infection symptoms by modulating intestinal probiotic and pathogenic bacteria balance

IF 2.3 2区农林科学 Q1 VETERINARY SCIENCES

Veterinary journal

Pub Date : 2025-04-03 DOI: 10.1016/j.tvjl.2025.106343

Jiajia Yang , Xuan Wang , Ying Chen , Ye He , Ping Li , Xin Wen , Bi Wang

Cecal coccidiosis is a severe and lethal parasitic disease affecting chickens, making the search for effective preventive agents free of contamination and drug resistance crucial for controlling this condition in poultry. Previous studies have demonstrated that the ChangQing compound has significant therapeutic effects against cecal coccidiosis; however, its potential as a preventive measure has yet to be evaluated. In this study, we established an experimental model for the prevention of cecal coccidiosis in chickens using the ChangQing compound for the first time. A comprehensive evaluation was performed on survival rates, relative weight gain, oocyst production, the anticoccidial index (ACI), immune parameters, parasitic tissue pathology, and microbial diversity in cecal contents. Results indicated that the ChangQing compound at a concentration of 5.0 g/L achieved an ACI of 178.10 in the Pre-Exposure Prophylaxis (PrEP) group and 173.12 in the Post-Exposure Prophylaxis (PEP) group. Compared to the positive control group, cecal lesions were reduced, and indices for the spleen, liver, and bursa of Fabricius increased in both experimental groups following ChangQing administration. Furthermore, levels of immune factors, IgA, IgG, and IgM significantly elevated. The abundance of beneficial bacteria, including Lactobacillus, Bacteroides, and Alistipes, increased in the 5.0 g/L ChangQing compound group, while potential pathogens like Escherichia-Shigella, Enterococcus, and norank_f_Oscillospiraceae were reduced. These findings offer critical data for coccidiosis prevention in chickens and lay a theoretical foundation for future research on the antiparasitic mechanisms of traditional Chinese medicine.

{"title":"ChangQing compound relieves Eimeria tenella infection symptoms by modulating intestinal probiotic and pathogenic bacteria balance","authors":"Jiajia Yang , Xuan Wang , Ying Chen , Ye He , Ping Li , Xin Wen , Bi Wang","doi":"10.1016/j.tvjl.2025.106343","DOIUrl":"10.1016/j.tvjl.2025.106343","url":null,"abstract":"<div><div>Cecal coccidiosis is a severe and lethal parasitic disease affecting chickens, making the search for effective preventive agents free of contamination and drug resistance crucial for controlling this condition in poultry. Previous studies have demonstrated that the ChangQing compound has significant therapeutic effects against cecal coccidiosis; however, its potential as a preventive measure has yet to be evaluated. In this study, we established an experimental model for the prevention of cecal coccidiosis in chickens using the ChangQing compound for the first time. A comprehensive evaluation was performed on survival rates, relative weight gain, oocyst production, the anticoccidial index (ACI), immune parameters, parasitic tissue pathology, and microbial diversity in cecal contents. Results indicated that the ChangQing compound at a concentration of 5.0 g/L achieved an ACI of 178.10 in the Pre-Exposure Prophylaxis (PrEP) group and 173.12 in the Post-Exposure Prophylaxis (PEP) group. Compared to the positive control group, cecal lesions were reduced, and indices for the spleen, liver, and bursa of Fabricius increased in both experimental groups following ChangQing administration. Furthermore, levels of immune factors, IgA, IgG, and IgM significantly elevated. The abundance of beneficial bacteria, including <em>Lactobacillus</em>, <em>Bacteroides</em>, and <em>Alistipes</em>, increased in the 5.0 g/L ChangQing compound group, while potential pathogens like <em>Escherichia-Shigella</em>, <em>Enterococcus</em>, and <em>norank_f_Oscillospiraceae</em> were reduced. These findings offer critical data for coccidiosis prevention in chickens and lay a theoretical foundation for future research on the antiparasitic mechanisms of traditional Chinese medicine.</div></div>","PeriodicalId":23505,"journal":{"name":"Veterinary journal","volume":"311 ","pages":"Article 106343"},"PeriodicalIF":2.3,"publicationDate":"2025-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143788958","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Diode Laser Epiglottidectomy (DLE) for management of epiglottic disease in 35 dogs

IF 2.3 2区农林科学 Q1 VETERINARY SCIENCES

Veterinary journal

Pub Date : 2025-04-03 DOI: 10.1016/j.tvjl.2025.106345

Davide De Lorenzi , Chiara Mantovani , Giulia Maggi , Enrico Bottero , Maria C. Marchesi

Veterinary literature on epiglottic disorders and their treatment in dogs is limited. The objective of this study is to report the clinical features, diagnosis, surgical treatment, and outcomes of dogs with epiglottic conditions treated using diode laser epiglottidectomy (DLE). This was a single-institution observational prospective study. Thirty-five dogs diagnosed with epiglottic disease underwent DLE. In all cases, an epiglottic disease was documented with combined laryngoscopic examination, when needed, fluoroscopy, computed tomography (CT) scan, and biopsy. Sub-total (SDLE) and total (TDLE) diode laser epiglottidectomy was performed under endoscopic guidance according to the diagnosis. Follow-up was performed by a re-examination visit and endoscopy, and telephone follow-up with the owner. The most common epiglottic disorder was epiglottic retroversion (ER) (57.1 %). SDLE was performed in 32/35 (91.4 %) dogs, while 3/35 (8.6 %) dogs underwent TDLE. Intraoperative complications occurred in 11.4 % surgeries, primarily involving bleeding. Postsurgical complications were reported in 8.5 % cases post-SDLE and were represented by transient airway obstruction caused by local edema. Follow-up (median 18 months, minimum 3 months – maximum 21 months) consultations revealed prolonged resolution of upper airway obstruction without signs of respiratory tract compromise or dysphagia. The surgical techniques described in this study have proven to be effective and minimally invasive for the treatment of epiglottic-related airway obstruction.

{"title":"Diode Laser Epiglottidectomy (DLE) for management of epiglottic disease in 35 dogs","authors":"Davide De Lorenzi , Chiara Mantovani , Giulia Maggi , Enrico Bottero , Maria C. Marchesi","doi":"10.1016/j.tvjl.2025.106345","DOIUrl":"10.1016/j.tvjl.2025.106345","url":null,"abstract":"<div><div>Veterinary literature on epiglottic disorders and their treatment in dogs is limited. The objective of this study is to report the clinical features, diagnosis, surgical treatment, and outcomes of dogs with epiglottic conditions treated using diode laser epiglottidectomy (DLE). This was a single-institution observational prospective study. Thirty-five dogs diagnosed with epiglottic disease underwent DLE. In all cases, an epiglottic disease was documented with combined laryngoscopic examination, when needed, fluoroscopy, computed tomography (CT) scan, and biopsy. Sub-total (SDLE) and total (TDLE) diode laser epiglottidectomy was performed under endoscopic guidance according to the diagnosis. Follow-up was performed by a re-examination visit and endoscopy, and telephone follow-up with the owner. The most common epiglottic disorder was epiglottic retroversion (ER) (57.1 %). SDLE was performed in 32/35 (91.4 %) dogs, while 3/35 (8.6 %) dogs underwent TDLE. Intraoperative complications occurred in 11.4 % surgeries, primarily involving bleeding. Postsurgical complications were reported in 8.5 % cases post-SDLE and were represented by transient airway obstruction caused by local edema. Follow-up (median 18 months, minimum 3 months – maximum 21 months) consultations revealed prolonged resolution of upper airway obstruction without signs of respiratory tract compromise or dysphagia. The surgical techniques described in this study have proven to be effective and minimally invasive for the treatment of epiglottic-related airway obstruction.</div></div>","PeriodicalId":23505,"journal":{"name":"Veterinary journal","volume":"311 ","pages":"Article 106345"},"PeriodicalIF":2.3,"publicationDate":"2025-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143788985","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The role of mentorship in veterinary practice: An empirical study of the Animal Health Mentoring Framework in Pakistan

IF 2.3 2区农林科学 Q1 VETERINARY SCIENCES

Veterinary journal

Pub Date : 2025-04-03 DOI: 10.1016/j.tvjl.2025.106348

Ujala Fatima Shan , Aneela Zameer Durrani , Muhammad Zahid Iqbal , Shakera Sadiq Gill , Esha Binte Iqbal , Sadia Sanaullah , Muhammad Aftab , Mahrukh Ahmed , Safdar Aman

This research introduced and ascertained the effectiveness of the Animal Health Mentoring Framework (AHMF) designed by Brooke, a global welfare organization, to develop the dual skills of ethical reasoning and the technical capabilities of field veterinarians in Pakistan. A longitudinal observational study was carried out on 100 veterinary professionals from six institutions in Lahore for six months by engaging them in a structured mentorship program. The AHMF targeted key developmental areas: welfare advocacy, clinical expertise, communication, kit content and maintenance, and clinical governance. Monthly assessments were carried out using the AHMF scoring tool, and the results were analyzed using the Friedman test across these five competencies. The results showed statistically significant improvements in all aspects. The welfare advocacy score increased from 1.13 at the start to 3.62 by the end of the study, clinical expertise improved from 8.26 to 12.51 while clinical governance enhanced from 0.14 to 1.12. Communication skills and kit management also improved significantly, although at a slightly slower rate. The average total AHMF score of the participants increased from 13.14 to 22.37, thus emphasizing all-rounded improvements in veterinary practices. The outcomes suggest that formal mentoring through the AHMF significantly enhances veterinary professionals' ethical and technical competencies, particularly in resource-poor settings. AHMF is a scalable, globally adaptable model, that can improve veterinary education, practice, and animal welfare, particularly in low and middle-income countries. This pioneering research sets a benchmark for professional development and offers a practical solution to elevate animal welfare and clinical expertise globally.

{"title":"The role of mentorship in veterinary practice: An empirical study of the Animal Health Mentoring Framework in Pakistan","authors":"Ujala Fatima Shan , Aneela Zameer Durrani , Muhammad Zahid Iqbal , Shakera Sadiq Gill , Esha Binte Iqbal , Sadia Sanaullah , Muhammad Aftab , Mahrukh Ahmed , Safdar Aman","doi":"10.1016/j.tvjl.2025.106348","DOIUrl":"10.1016/j.tvjl.2025.106348","url":null,"abstract":"<div><div>This research introduced and ascertained the effectiveness of the Animal Health Mentoring Framework (AHMF) designed by Brooke, a global welfare organization, to develop the dual skills of ethical reasoning and the technical capabilities of field veterinarians in Pakistan. A longitudinal observational study was carried out on 100 veterinary professionals from six institutions in Lahore for six months by engaging them in a structured mentorship program. The AHMF targeted key developmental areas: welfare advocacy, clinical expertise, communication, kit content and maintenance, and clinical governance. Monthly assessments were carried out using the AHMF scoring tool, and the results were analyzed using the Friedman test across these five competencies. The results showed statistically significant improvements in all aspects. The welfare advocacy score increased from 1.13 at the start to 3.62 by the end of the study, clinical expertise improved from 8.26 to 12.51 while clinical governance enhanced from 0.14 to 1.12. Communication skills and kit management also improved significantly, although at a slightly slower rate. The average total AHMF score of the participants increased from 13.14 to 22.37, thus emphasizing all-rounded improvements in veterinary practices. The outcomes suggest that formal mentoring through the AHMF significantly enhances veterinary professionals' ethical and technical competencies, particularly in resource-poor settings. AHMF is a scalable, globally adaptable model, that can improve veterinary education, practice, and animal welfare, particularly in low and middle-income countries. This pioneering research sets a benchmark for professional development and offers a practical solution to elevate animal welfare and clinical expertise globally.</div></div>","PeriodicalId":23505,"journal":{"name":"Veterinary journal","volume":"311 ","pages":"Article 106348"},"PeriodicalIF":2.3,"publicationDate":"2025-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143789041","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Feasibility and cartilage injury of an all-inside arthroscopic meniscal repair application in a canine cadaveric study

IF 2.3 2区农林科学 Q1 VETERINARY SCIENCES

Veterinary journal

Pub Date : 2025-04-02 DOI: 10.1016/j.tvjl.2025.106339

David Michalik , Robert A. Pettitt , Alexander Tichy , Eva Schnabl-Feichter

Meniscal injury is the most common comorbidity in canine stifles with cranial cruciate ligament (CCL) pathology. Arthroscopic repair for acute tears can be technically challenging and is rarely described. This study evaluates the feasibility of using an all-inside arthroscopic meniscal repair device, Arthrex Meniscal Cinch II™ (MCII) in canine cadaveric stifles and the associated risks of iatrogenic vascular injury (IVI) and cartilage injury (ICI). 20 healthy paired canine cadaveric stifles (25 – 45 kg BW) were divided randomly into two groups of 10. Both groups received transection of the CCL via mini arthrotomy, joint distraction and diagnostic arthroscopy with meniscal probing. No further procedures were performed in the control group whereas the implant group underwent placement of the MCII-implants in the caudal horn of the medial meniscus. Angiography of the femoral artery was performed for each limb before and after operation and vascular trauma was assessed on radiographs. After disarticulation, the cartilage was stained via Indian ink assay and underwent blinded scoring for ICI. Implants were evaluated for desired position. Correct position of the MCII-implants was achieved in all stifles. No IVI was detected. Implant placement created more ICI on the medial femoral condyle (1.33 mm²) than arthroscopy alone (0.15 mm²) (p = 0.03). Implant associated complications occurred in 30 %. Arthroscopic use of MCII is feasible and carries minimal risk for vascular damage. Despite joint distraction, implant placement using the MCII is associated with significant cartilage damage. Although technically challenging, all-inside meniscal repair appears to carry limited risk in canine patients over 25 kg bodyweight.

{"title":"Feasibility and cartilage injury of an all-inside arthroscopic meniscal repair application in a canine cadaveric study","authors":"David Michalik , Robert A. Pettitt , Alexander Tichy , Eva Schnabl-Feichter","doi":"10.1016/j.tvjl.2025.106339","DOIUrl":"10.1016/j.tvjl.2025.106339","url":null,"abstract":"<div><div>Meniscal injury is the most common comorbidity in canine stifles with cranial cruciate ligament (CCL) pathology. Arthroscopic repair for acute tears can be technically challenging and is rarely described. This study evaluates the feasibility of using an all-inside arthroscopic meniscal repair device, Arthrex Meniscal Cinch II™ (MCII) in canine cadaveric stifles and the associated risks of iatrogenic vascular injury (IVI) and cartilage injury (ICI). 20 healthy paired canine cadaveric stifles (25 – 45 kg BW) were divided randomly into two groups of 10. Both groups received transection of the CCL via mini arthrotomy, joint distraction and diagnostic arthroscopy with meniscal probing. No further procedures were performed in the control group whereas the implant group underwent placement of the MCII-implants in the caudal horn of the medial meniscus. Angiography of the femoral artery was performed for each limb before and after operation and vascular trauma was assessed on radiographs. After disarticulation, the cartilage was stained via Indian ink assay and underwent blinded scoring for ICI. Implants were evaluated for desired position. Correct position of the MCII-implants was achieved in all stifles. No IVI was detected. Implant placement created more ICI on the medial femoral condyle (1.33 mm<sup>2</sup>) than arthroscopy alone (0.15 mm<sup>2</sup>) (p = 0.03). Implant associated complications occurred in 30 %. Arthroscopic use of MCII is feasible and carries minimal risk for vascular damage. Despite joint distraction, implant placement using the MCII is associated with significant cartilage damage. Although technically challenging, all-inside meniscal repair appears to carry limited risk in canine patients over 25 kg bodyweight.</div></div>","PeriodicalId":23505,"journal":{"name":"Veterinary journal","volume":"311 ","pages":"Article 106339"},"PeriodicalIF":2.3,"publicationDate":"2025-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143777269","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Pharmacokinetic evaluation of meloxicam following intravenous and intramuscular administration in Crocodylus siamensis, a freshwater crocodile

IF 2.3 2区农林科学 Q1 VETERINARY SCIENCES

Veterinary journal

Pub Date : 2025-04-01 DOI: 10.1016/j.tvjl.2025.106342

Seavchou Laut , Saranya Poapolathep , Kraisiri Khidkhan , Narumol Klangkaew , Napassorn Phaochoosak , Tara Wongwaipairoj , Mario Giorgi , Pedro Marin , Elisa Escudero , Amnart Poapolathep

The pharmacokinetics of meloxicam (MLX) remain largely unexplored in reptiles, particularly in Siamese crocodiles (Crocodylus siamensis). This study characterized the pharmacokinetic profiles of MLX following intravenous (IV) and intramuscular (IM) administration in Siamese crocodiles. Fifteen Siamese crocodiles were divided into three groups (n=5) using a randomization procedure according to a parallel study design. MLX was administered IV at 0.2 mg/kg b.w. or IM at two different doses (0.2 mg/kg b.w. or 0.4 mg/kg b.w.). Plasma concentrations of MLX were measured using a validated high-performance liquid chromatography method with UV detection. The pharmacokinetic parameters were analyzed using a non-compartment model. The elimination half-life (t_1/2λz) was long for all administration routes, with values of 132.34 hr (IV), 121.35 hr (IM 0.2 mg/kg b.w.), and 181.44 hr (IM 0.4 mg/kg b.w.). The volumes of distribution (Vd) and clearance (Cl) after IV administration were 104.59 mL/kg and 0.55 mL/hr/kg, respectively. Based on these results, there was an extended t_1/2λz of MLX in this species of freshwater crocodiles, highlighting significant differences in drug disposition compared to other reptilian and non-reptilian species. The findings contribute to an understanding of MLX pharmacokinetics in this animal species, and emphasize that the selection of the optimal dose of MLX should be considered based on disposition kinetics, efficacy, safety, and species-specific differences. Further investigation is required to identify the effective plasma concentration, which is critical for establishing the appropriate dose for the management of pain and inflammation.

{"title":"Pharmacokinetic evaluation of meloxicam following intravenous and intramuscular administration in Crocodylus siamensis, a freshwater crocodile","authors":"Seavchou Laut , Saranya Poapolathep , Kraisiri Khidkhan , Narumol Klangkaew , Napassorn Phaochoosak , Tara Wongwaipairoj , Mario Giorgi , Pedro Marin , Elisa Escudero , Amnart Poapolathep","doi":"10.1016/j.tvjl.2025.106342","DOIUrl":"10.1016/j.tvjl.2025.106342","url":null,"abstract":"<div><div>The pharmacokinetics of meloxicam (MLX) remain largely unexplored in reptiles, particularly in Siamese crocodiles (<em>Crocodylus siamensis</em>). This study characterized the pharmacokinetic profiles of MLX following intravenous (IV) and intramuscular (IM) administration in Siamese crocodiles. Fifteen Siamese crocodiles were divided into three groups (n=5) using a randomization procedure according to a parallel study design. MLX was administered IV at 0.2mg/kg b.w. or IM at two different doses (0.2mg/kg b.w. or 0.4mg/kg b.w.). Plasma concentrations of MLX were measured using a validated high-performance liquid chromatography method with UV detection. The pharmacokinetic parameters were analyzed using a non-compartment model. The elimination half-life (t<sub>1/2λz</sub>) was long for all administration routes, with values of 132.34hr (IV), 121.35hr (IM 0.2mg/kg b.w.), and 181.44hr (IM 0.4mg/kg b.w.). The volumes of distribution (Vd) and clearance (Cl) after IV administration were 104.59mL/kg and 0.55mL/hr/kg, respectively. Based on these results, there was an extended t<sub>1/2λz</sub> of MLX in this species of freshwater crocodiles, highlighting significant differences in drug disposition compared to other reptilian and non-reptilian species. The findings contribute to an understanding of MLX pharmacokinetics in this animal species, and emphasize that the selection of the optimal dose of MLX should be considered based on disposition kinetics, efficacy, safety, and species-specific differences. Further investigation is required to identify the effective plasma concentration, which is critical for establishing the appropriate dose for the management of pain and inflammation.</div></div>","PeriodicalId":23505,"journal":{"name":"Veterinary journal","volume":"311 ","pages":"Article 106342"},"PeriodicalIF":2.3,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143768453","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

High prevalence of virulence genes and multi-drug resistance in Pasteurella multocida from goats in Sichuan, China

IF 2.3 2区农林科学 Q1 VETERINARY SCIENCES

Veterinary journal

Pub Date : 2025-03-31 DOI: 10.1016/j.tvjl.2025.106344

Shenglin Li , Qibing Gu , Benrun Li, Kehamo Abi, Falong Yang

Pasteurella multocida is one of the most important pathogens that infect goats, causing serious economic losses in the goat breeding industry. To understand the biological characteristics of P. multocida from goats, a comprehensive characterization of bacteria isolated from 342 nasal swabs and 8 lung tissue samples from goat farms in Sichuan, China, was performed. A total of 34 isolates were assigned to one capsular type, D, and one lipopolysaccharide (LPS) genotype, L3, indicating that the D: L3 was the predominant serotype in goat farms. In the 34 isolates, multiple virulence-related genes were identified, with a detection rate of 100 % (34/34) for the genes ompA, ompH, oma87, exbB, and exbD. It is noteworthy that the prevalence of the toxA gene, which encodes the P. multocida toxin (PMT), was found to be 85.2 % (29/34). Furthermore, antimicrobial susceptibility testing indicated a high prevalence of multidrug resistance, with resistance rates of 41.1 % for ampicillin, 38.2 % for tetracycline, and 32.3 % for kanamycin. Overall, this study provides a foundational understanding of the epidemiology and antimicrobial resistance of P. multocida in goats, offering insights for future prevention and control measures.

{"title":"High prevalence of virulence genes and multi-drug resistance in Pasteurella multocida from goats in Sichuan, China","authors":"Shenglin Li , Qibing Gu , Benrun Li, Kehamo Abi, Falong Yang","doi":"10.1016/j.tvjl.2025.106344","DOIUrl":"10.1016/j.tvjl.2025.106344","url":null,"abstract":"<div><div><em>Pasteurella multocida</em> is one of the most important pathogens that infect goats, causing serious economic losses in the goat breeding industry. To understand the biological characteristics of <em>P. multocida</em> from goats, a comprehensive characterization of bacteria isolated from 342 nasal swabs and 8 lung tissue samples from goat farms in Sichuan, China, was performed. A total of 34 isolates were assigned to one capsular type, D, and one lipopolysaccharide (LPS) genotype, L3, indicating that the D: L3 was the predominant serotype in goat farms. In the 34 isolates, multiple virulence-related genes were identified, with a detection rate of 100 % (34/34) for the genes <em>ompA</em>, <em>ompH</em>, <em>oma87</em>, <em>exbB</em>, and <em>exbD</em>. It is noteworthy that the prevalence of the <em>toxA</em> gene, which encodes the <em>P. multocida</em> toxin (PMT), was found to be 85.2 % (29/34). Furthermore, antimicrobial susceptibility testing indicated a high prevalence of multidrug resistance, with resistance rates of 41.1 % for ampicillin, 38.2 % for tetracycline, and 32.3 % for kanamycin. Overall, this study provides a foundational understanding of the epidemiology and antimicrobial resistance of <em>P. multocida</em> in goats, offering insights for future prevention and control measures.</div></div>","PeriodicalId":23505,"journal":{"name":"Veterinary journal","volume":"311 ","pages":"Article 106344"},"PeriodicalIF":2.3,"publicationDate":"2025-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143768471","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development of an in-home screening tool for canine periodontitis.

IF 2.3 2区农林科学 Q1 VETERINARY SCIENCES

Veterinary journal

Pub Date : 2025-03-31 DOI: 10.1016/j.tvjl.2025.106341

C Wallis, A Ruparell, M Soltero-Rivera, C Harvey, L Carvell-Miller, A Colyer, R M Reynolds, T S McKee, T Mills, P J Bergman, P Watson, L J Holcombe

Periodontal disease is a common condition in dogs but frequently goes unnoticed by owners. The objective of this study was to develop and validate an in-home screening tool for the detection of canine periodontitis based on the identification of bacterial biomarkers in supragingival plaque. A series of studies were undertaken to establish methods for collecting dental plaque and stabilising its bacterial profile. These were used by owners to collect supragingival plaque samples in a home setting from their dogs (n=127) whose periodontal health state had been determined by a veterinarian. Samples were screened for the presence of a bacterial biomarker of periodontitis using quantitative polymerase chain reaction (qPCR). Evaluation of six swabs (10 samples each) for plaque collection indicated the CytoSoft™ cytology brush yielded the highest amount of bacterial DNA, determined using a universal bacterial qPCR assay. Evaluation of six stabilisation methods (10-11 samples each) showed no significant differences between air-dried swabs stored at ambient temperatures and those maintained at -80^oC for 5 days. qPCR data from plaque samples collected by owners indicated an accuracy at predicting disease of 80.9%, a specificity of 80.0% and a sensitivity of 80.9%. Collection of supragingival plaque by dog owners using the CytoSoft™ cytology brush and postage to a laboratory for qPCR analysis is a practical method of screening for canine periodontitis. The simple, cost-effective method is a viable proposition to enable the detection of periodontitis in client-owned dogs ultimately improving their oral health and quality of life.

{"title":"Development of an in-home screening tool for canine periodontitis.","authors":"C Wallis, A Ruparell, M Soltero-Rivera, C Harvey, L Carvell-Miller, A Colyer, R M Reynolds, T S McKee, T Mills, P J Bergman, P Watson, L J Holcombe","doi":"10.1016/j.tvjl.2025.106341","DOIUrl":"https://doi.org/10.1016/j.tvjl.2025.106341","url":null,"abstract":"<p><p>Periodontal disease is a common condition in dogs but frequently goes unnoticed by owners. The objective of this study was to develop and validate an in-home screening tool for the detection of canine periodontitis based on the identification of bacterial biomarkers in supragingival plaque. A series of studies were undertaken to establish methods for collecting dental plaque and stabilising its bacterial profile. These were used by owners to collect supragingival plaque samples in a home setting from their dogs (n=127) whose periodontal health state had been determined by a veterinarian. Samples were screened for the presence of a bacterial biomarker of periodontitis using quantitative polymerase chain reaction (qPCR). Evaluation of six swabs (10 samples each) for plaque collection indicated the CytoSoft™ cytology brush yielded the highest amount of bacterial DNA, determined using a universal bacterial qPCR assay. Evaluation of six stabilisation methods (10-11 samples each) showed no significant differences between air-dried swabs stored at ambient temperatures and those maintained at -80<sup>o</sup>C for 5 days. qPCR data from plaque samples collected by owners indicated an accuracy at predicting disease of 80.9%, a specificity of 80.0% and a sensitivity of 80.9%. Collection of supragingival plaque by dog owners using the CytoSoft™ cytology brush and postage to a laboratory for qPCR analysis is a practical method of screening for canine periodontitis. The simple, cost-effective method is a viable proposition to enable the detection of periodontitis in client-owned dogs ultimately improving their oral health and quality of life.</p>","PeriodicalId":23505,"journal":{"name":"Veterinary journal","volume":" ","pages":"106341"},"PeriodicalIF":2.3,"publicationDate":"2025-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143773576","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0