Veterinary journal最新文献

Diaphragm ultrasound as a tool to study respiratory function in dogs: a validation study correlating sonographic findings with oesophageal pressure and spirometry during mechanical ventilation and spontaneous breathing.

IF 2.3 2区农林科学 Q1 VETERINARY SCIENCES

Veterinary journal

Pub Date : 2025-03-04 DOI: 10.1016/j.tvjl.2025.106323

Ilaria Tommasi, Stefano Oricco, Daniele Medico, Diego Sarotti, Roberto Rabozzi, Paolo Franci

This prospective observational study investigated the correlation between diaphragmatic ultrasound indices (movement and thickening fraction) and trans-diaphragmatic, oesophageal, or transpulmonary pressures, as well as tidal volumes, in anaesthetised dogs. Fifty-seven dogs of various breeds undergoing different procedures were studied under mechanical ventilation at three tidal volumes (5, 10, and 20ml/kg) and during spontaneous breathing post-ventilation. Diaphragmatic movement was evaluated with a microconvex probe in M-mode using the transversal plane during mechanical ventilation, and both transversal and longitudinal planes during spontaneous breathing. Diaphragmatic thickening fraction was assessed with a linear probe using M-mode and B-mode scans during spontaneous breathing. Oesophageal and gastric pressures were measured via a nasogastric feeding tube, with data collected across three respiratory cycles. Transpulmonary pressure strongly correlated with diaphragmatic excursion during mechanical ventilation (rho =.81; P <.001). Moderate correlations were observed between tidal volume and diaphragmatic excursion during spontaneous breathing (rho =.72 for longitudinal and.70 for transversal planes; P <.001). Fair correlations were found between oesophageal pressure and diaphragmatic slope during spontaneous breathing (rho =.56 for longitudinal and.46 for transversal planes; P <.001). Longitudinal and transversal planes strongly correlated for excursion (rho =.94; P <.001) and slope (rho =.91; P <.001), with no fixed bias. Diaphragmatic thickening fraction did not correlate with oesophageal pressure (rho =.26 for B-mode and.129 for M-mode; P =.051 and.34, respectively). These findings highlight ultrasound as a promising tool for assessing diaphragmatic function in veterinary medicine.

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引用次数: 0

Dried distillers’ grains with solubles as a key feed ingredient in dairy cow diets: Implications for colostrum quality and calf immunity

IF 2.3 2区农林科学 Q1 VETERINARY SCIENCES

Veterinary journal

Pub Date : 2025-02-22 DOI: 10.1016/j.tvjl.2025.106317

Andrzej Zachwieja , Ewa Pecka-Kiełb , Katarzyna Humienna , Anna Zielak-Steciwko , Bożena Króliczewska , Jowita Kaszuba , Maciej Adamski , Joanna Tumanowicz , Martyna Wilk

The study evaluated the effect of incorporating dried distillers’ grains with solubles (DDGS) into the cow’s diet during the dry period on colostrum quality, concentrations of protein, and immunoglobulin G (IgG) in the serum of cows and calves. Three weeks prior to the expected calving date, cows were selected and assigned to four groups, each comprising 15 animals. The standard group (Group I) was fed a standard total mixed ration (TMR). Groups II, III, and IV received TMR supplemented with DDGS at rates of 1 kg, 2 kg, and 3 kg per head per day, respectively. Colostrum and blood samples were collected from all cows. Blood samples were also obtained from calves on the 3rd and 30th days of life. Analyses of colostrum: basic composition, amino acid profile, IgG, IgA, IgM, protein fraction distribution, and concentrations of Ca, P, Mg, K, and Na. In blood samples, total protein content and IgG levels were determined. Colostrum from cows in groups I and IV had decreased lactoferrin level compared to group II. In group III, an increase in IgG and total protein levels was observed. Regardless of the DDGS supplementation levels in maternal feed rations, an increase in total protein and IgG levels was noted in the serum of calves on both the 3rd and 30th days of age. These findings suggest that the use of DDGS in cow’s diets during the pre-calving period appears to enhance colostrum quality, and would seem to increase immunoglobulin levels and improve passive immunity in calves.

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引用次数: 0

Serum 25-hydroxyvitamin D and 24,25-dihydroxyvitamin D are decreased in dogs with sinonasal aspergillosis

IF 2.3 2区农林科学 Q1 VETERINARY SCIENCES

Veterinary journal

Pub Date : 2025-02-18 DOI: 10.1016/j.tvjl.2025.106318

Arnaud Snoeck , Jared A. Jaffey , Frédéric Billen , Nina F. Rodrigues , Stéphanie Peeters , Caroline Le Goff , Etienne Cavalier , Dominique Peeters , Cécile Clercx

Canine sinonasal aspergillosis (SNA) is a poorly understood disease and remains a challenge to treat. Hypovitaminosis D is associated with many infectious diseases in humans and Vitamin D (VitD) deficiency in experimental mice decreases resistance to Aspergillus fumigatus. The objective of this study was to determine whether dogs with SNA have different VitD metabolite concentrations compared to healthy dogs (HD) and dogs with other nasal conditions and if those concentrations change after cure for SNA dogs. Twenty-two dogs with SNA, 12 HD, 9 dogs with lymphoplasmacytic rhinitis (LPR) and 10 dogs with nasal neoplasia (NN) were included. Serum 25-hydroxyvitamin D₂ (25(OH)D₂), 25-hydroxyvitamin D₃ (25(OH)D₃), 24,25-dihydroxyvitamin D₃ (24,25(OH)₂D₃), 3-epimer-25-hydroxyvitamin D₃ (3-epi-25(OH)D₃) concentrations were measured by a certified liquid chromatography tandem mass spectrometry method at time of diagnosis or revisit. Twelve SNA dogs were available for serial blood collection until cure. Serum 25(OH)D and 24,25(OH)₂D₃ were lower in dogs with SNA (mean ± standard deviation; 23 ng/ml ± 7.3 and 10.2 ng/ml ± 4.2, respectively) than in HD (34.1 ng/ml ± 7.5; P = 0.007 and 18.2 ng/ml ± 5.4; P = 0.002) while there was no difference among the other groups. Cured SNA dogs had higher serum 25(OH)D concentrations (27.7 ng/ml ± 9.4) compared to before treatment (23.1 ng/ml ± 7.7; P = 0.0002). These results further support the rationale that VitD may play a role in the complex SNA pathophysiology. Whether lower VitD status contributes to the development of the disease or is a consequence of it is unknown.

犬鼻窦曲霉菌病（SNA）是一种人们知之甚少的疾病，在治疗上仍是一项挑战。维生素 D 不足与人类的许多传染病有关，而实验小鼠缺乏维生素 D（VitD）会降低对曲霉菌的抵抗力。本研究的目的是确定与健康犬（HD）和患有其他鼻部疾病的犬相比，患有 SNA 的犬的维生素 D 代谢物浓度是否不同，以及 SNA 犬治愈后这些浓度是否会发生变化。这项研究共纳入了 22 只 SNA 狗、12 只 HD 狗、9 只淋巴浆细胞性鼻炎 (LPR) 狗和 10 只鼻腔肿瘤 (NN) 狗。在确诊或复诊时，采用经认证的液相色谱串联质谱法测量血清中 25-羟维生素 D2 (25(OH)D2)、25-羟维生素 D3 (25(OH)D3)、24,25-二羟维生素 D3 (24,25(OH)2D3)、3-表-25-羟维生素 D3 (3-表-25(OH)D3)的浓度。12 只 SNA 狗在治愈前可进行连续采血。SNA犬的血清25(OH)D和24,25(OH)2D3（平均值±标准偏差；分别为23ng/mL ± 7.3和10.2ng/mL ± 4.2）低于HD犬（34.1ng/mL ± 7.5；P = 0.007和18.2ng/mL ± 5.4；P = 0.002），而其他组之间没有差异。与治疗前（23.1ng/mL ± 7.7; P = 0.0002）相比，治愈的 SNA 狗的血清 25(OH)D 浓度更高（27.7ng/mL ± 9.4）。这些结果进一步支持了 VitD 可能在复杂的 SNA 病理生理学中发挥作用的观点。VitD水平较低是导致疾病发展的原因，还是疾病发展的结果，目前尚不清楚。

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引用次数: 0

Equine adipose tissue-derived extracellular vesicles enhance adipose mesenchymal stem cell survival ex vivo

IF 2.3 2区农林科学 Q1 VETERINARY SCIENCES

Veterinary journal

Pub Date : 2025-02-18 DOI: 10.1016/j.tvjl.2025.106319

Dongsheng Li , Xin Wang , Junyong Yao , Shengfeng Chen

Adipose tissue-derived extracellular vesicles (ATEVs) have garnered attention for their roles in intercellular communication and regulation. This study presents the first investigation of equine adipose tissue, with ATEV being extracted concurrently during the isolation of adipose-derived mesenchymal stem cells (ADSCs). Through CCK-8 cell proliferation assays and scratch migration assays, a significant promotional effect of ATEV on ADSCs was observed, which not only accelerated the proliferation rate of the stem cells but also enhanced their migratory capacity. The application of Trypan Blue exclusion and live-dead cell staining further demonstrated the positive effects of ATEV on maintaining the viability of ADSCs during ex vivo storage. These findings provide new insights into the potential applications of ATEV in stem cell therapy, tissue repair, and regenerative medicine.

引用次数: 0

Digital dermatitis in dairy cattle in Egypt: Herd-level risk factors and Treponema spp. prevalence across lesion M-scores 埃及奶牛的数字皮炎：群体水平的危险因素和密螺旋体在病变m评分中的患病率。

IF 2.3 2区农林科学 Q1 VETERINARY SCIENCES

Veterinary journal

Pub Date : 2025-02-01 DOI: 10.1016/j.tvjl.2024.106289

S.E. Salem , A. Monir , H.M. Abdallah , A.F. Mahmoud , H.A. Elsheikh , W. Refaai , A.B. Ahmed , H.E.M. Elsheikh , M. El-Sheikh , A. Mesalam

Digital dermatitis (DD) is an infectious disease of the digital skin of dairy cows that is associated with compromised animal welfare and significant economic losses. The hind feet of 16,098 dairy cows from 55 herds were examined in the milking parlor, and DD lesions identified were classified using the M-score system and swabbed for PCR testing. Swabs were also collected from hind feet with normal digital skin for comparison. Data on farm construction and lameness prevention measures were collected through direct observation and in-person interviews with farm managers. Herd-level risk factors for DD were investigated using mixed-effects logistic regression models, with Farm ID included as a random-effects variable. The average within-herd and herd prevalence of DD were 6.4 % (95 % CI: 4.9–8.0 %) and 92.7 % (n = 51, 95 % CI: 85.9–99.6 %), respectively. Among the investigated DD lesions, DD-associated treponemes were identified at frequencies of 87 % for Treponema spp., 87.5 % for T. medium, 86.2 % for T. phagedenis-like, and 78.3 % for T. pedis. Additionally, Fusobacterium necrophorum and Dichelobacter nodosus were identified at frequencies of 37 % and 15.4 %, respectively. Samples from normal digital skin exhibited substantially lower frequencies for all bacterial species investigated. Risk factors significantly associated with DD in the final multivariable model included practice of preventive early lactation trimming (OR=0.45, 95 % CI: 0.26–0.78) and the presence of an abrasive bedding on farms (OR=1.6, 95 % CI: 1.01–2.6). This comprehensive molecular and epidemiological analysis enhances understanding of DD etiology in Egypt and contributes to the development of effective control programs to improve cow welfare and support the sustainability of milk production.

数字皮炎（DD）是奶牛数字皮肤的一种传染性疾病，与动物福利受损和重大经济损失有关。我们在挤奶厅对 55 个牧场的 16,098 头奶牛的后足进行了检查，采用 M 评分系统对发现的 DD 病变进行了分类，并采集拭子进行 PCR 检测。同时还采集了后足正常数字皮肤的拭子进行对比。通过直接观察和与牧场管理人员面谈，收集有关牧场建设和跛足预防措施的数据。采用混合效应逻辑回归模型研究牛群层面的跛足风险因素，并将猪场 ID 作为随机效应变量。牛群内和牛群中 DD 的平均发病率分别为 6.4% (95% CI: 4.9-8.0%) 和 92.7% (n=51, 95% CI: 85.9-99.6%)。在所调查的 DD 病变中，与 DD 相关的颤抖病原菌的频率分别为：颤抖病原菌 87%、中型颤抖病原菌 87.5%、类颤抖病原菌 86.2%、足型颤抖病原菌 78.3%。此外，还发现了坏死镰刀菌（Fusobacterium necrophorum）和结节双歧杆菌（Dichelobacter nodosus），发现率分别为 37% 和 15.4%。正常数字皮肤样本中所有细菌种类的检出率都低得多。在最终的多变量模型中，与 DD 明显相关的风险因素包括泌乳早期的预防性修剪（OR=0.45，95% CI：0.26-0.78）和猪场中是否存在研磨垫料（OR=1.6，95% CI：1.01-2.6）。这项全面的分子和流行病学分析加深了人们对埃及DD病因的了解，有助于制定有效的控制计划，改善奶牛福利，支持牛奶生产的可持续性。

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引用次数: 0

Observer reliability in counting erythrocyte ghost cells and impact of short-term storage of canine and feline blood samples 红细胞鬼影细胞计数的观察者可靠性及其对犬、猫血样短期储存的影响。

IF 2.3 2区农林科学 Q1 VETERINARY SCIENCES

Veterinary journal

Pub Date : 2025-02-01 DOI: 10.1016/j.tvjl.2024.106294

Asger Lundorff Jensen, Kamilja Sayed Gammal Bruun, Stephanie Heimann, Rebecca Langhorn, Anne K.H. Krogh

The presence of erythrocyte ghost cells (EG) in blood smears indicates intravascular haemolysis or in-vitro haemolysis. However, observer reliability in detection of EG has not been documented. Immediate blood smear preparation is advised but may not always be practical. This study investigated the reliability of EG detection and the impact of delayed blood smear preparation. Forty blood smears from dogs and cats were evaluated twice by four observers. EG development was studied in 59 dogs and nine cats, with smears prepared at 0, 2, and 24 h post-collection. Intra- and inter-observer reliability, as assessed using Krippendorff’s Alpha, ranged from 0.567 and 0.949, and 0.522 and 0.848, respectively, indicating moderate to substantial agreement. No significant increase in EG occurrence was noted between 0 and 2 h, but a significant increase was observed at 24 h. The findings suggest that EG can be reliably detected and that blood smears for the evaluation of EG can be prepared up to 2 h after blood collection without compromising detection of EG.

血涂片中出现红细胞鬼影细胞（EG）提示血管内溶血或体外溶血。然而，观察者在EG检测中的可靠性还没有文献记载。建议立即准备血液涂片，但可能并不总是实际的。本研究探讨EG检测的可靠性及延迟血涂片制备的影响。四名观察员对来自狗和猫的40份血液涂片进行了两次评估。研究了59只狗和9只猫的EG发育，并在采集后0、2和24小时制备了涂片。使用Krippendorff's Alpha评估的观察者内部和观察者之间的信度范围分别为0.567和0.949,0.522和0.848，表明中度到实质性的一致。0 ~ 2h间EG发生率无明显增高，但24h时明显增高。研究结果表明，可以可靠地检测EG，并且可以在采血后2小时内制备用于评估EG的血涂片，而不会影响EG的检测。

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引用次数: 0

Effects of probiotic and yeast extract supplement on liver functionality index and metabolic parameters in transition period of dairy cattle 添加益生菌和酵母提取物对过渡期奶牛肝脏功能指数和代谢参数的影响。

IF 2.3 2区农林科学 Q1 VETERINARY SCIENCES

Veterinary journal

Pub Date : 2025-02-01 DOI: 10.1016/j.tvjl.2024.106280

Mohammad Salahlou , Ali Hajimohammadi , Saeed Nazifi , Abbas Rowshan-Ghasrodashti , Mohammad Nikzad , Ahmadreza Mirzaei

This research sought to evaluate the potential effects of probiotics and yeast cell wall (YCW) supplements on the liver functionality index (LFI) and metabolic parameters of dairy cattle throughout the transitional period. A cohort of forty dry cows was randomly divided into four groups, namely the probiotic group (Pr) receiving a basal diet combined with a blend of Bacillus subtilis, Bacillus lechiniformis, Streptococcus Thermophilis, and Enterococcus faecium; the YCW group receiving a basal diet enriched with Saccharomyces cerevisiae; the probiotic and yeast cell wall extract group (P & Y) receiving a basal diet supplemented with a mixture of probiotic and yeast cell wall extract; and the control group adhering to the basal diet. The intervention was initiated 21 days before calving and persisted until 28 days post-calving, except for the control group. The study entailed the collection of blood samples at four sampling times, encompassing 21 days preceding calving, seven days before calving, seven days post-calving, and four weeks post-calving. Multiple biochemical parameters were assessed, including urea, blood urea nitrogen (BUN), Gamma-glutamyl transferase (GGT), total bilirubin (TB), albumin, total protein (TP), globulin, glucose, triglyceride, cholesterol, and liver functionality index. The results showed that the Pr group exhibited reduced average levels of GGT and glucose compared to the control group (P < 0.05). Similarly, the P & Y group demonstrated lower average BUN, TB, and cholesterol levels than the control (P < 0.05). Notably, the LFI exhibited a discernible trend towards elevation in the Pr group compared to the control group (P = 0.007) and the P & Y group (P = 0.007). In essence, supplementation of YCW and probiotics is associated with advantageous effects on metabolic parameters and liver function.

本研究旨在评价饲粮中添加益生菌和酵母细胞壁（YCW）对过渡期奶牛肝脏功能指数（LFI）和代谢参数的潜在影响。选取40头奶牛，随机分为4组，即益生菌组（Pr）饲喂混合枯草芽孢杆菌、芽孢杆菌、嗜热链球菌和粪肠球菌的基础饲粮；YCW组饲喂富含酿酒酵母的基础饲粮；益生菌和酵母细胞壁提取物组（P & Y）在基础饲粮中添加益生菌和酵母细胞壁提取物的混合物；而对照组则坚持基础饮食。除对照组外，干预于产犊前21天开始，持续至产犊后28天。该研究分四次采集血液样本，分别是产犊前21天、产犊前7天、产犊后7天和产犊后四周。评估多项生化指标，包括尿素、血尿素氮（BUN）、γ -谷氨酰转移酶（GGT）、总胆红素（TB）、白蛋白、总蛋白（TP）、球蛋白、葡萄糖、甘油三酯、胆固醇和肝功能指数。结果表明，与对照组相比，Pr组GGT和葡萄糖的平均水平降低（P < 0.05）。同样，P & Y组的平均BUN、TB和胆固醇水平低于对照组（P < 0.05）。值得注意的是，与对照组（P = 0.007）和P & Y组（P = 0.007）相比，Pr组的LFI有明显的升高趋势。从本质上讲，补充YCW和益生菌对代谢参数和肝功能有有利的影响。

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引用次数: 0

Quantitative proteomics analysis of cerebrospinal fluid reveals putative protein biomarkers for canine non-infectious meningoencephalomyelitis 脑脊液定量蛋白质组学分析揭示了犬非传染性脑膜脑脊髓炎的推定蛋白质生物标志物。

IF 2.3 2区农林科学 Q1 VETERINARY SCIENCES

Veterinary journal

Pub Date : 2025-02-01 DOI: 10.1016/j.tvjl.2024.106285

M. Aradillas-Pérez , E.M. Espinosa-López , B. Ortiz-Guisado , E.M. Martín-Suárez , G. Gómez-Baena , A. Galán-Rodríguez

Accurate ante-mortem diagnosis of non-infectious meningoencephalomyelitis (NIME) in dogs is challenging due to the similarity of clinical presentations, imaging findings, and cerebrospinal fluid (CSF) analysis results with other diseases. This study aimed to apply state-of-the-art quantitative proteomic technology to identify novel biomarkers for NIME. Serum and CSF samples from 11 dogs were included, with the control group consisting of patients presenting with intervertebral disc disease (IVDD, n = 6) and the study group consisting of dogs suffering from NIME (n = 5). Mass spectrometry-based quantitative proteomics revealed a set of 36 proteins with significant differential abundance in CSF samples. Up-regulated proteins in NIME CSF included immunoglobulins, inter-alpha-trypsin inhibitor heavy chain 2, acid sphingomyelinase-like phosphodiesterase, and chitinase 3-like protein 1, all associated with immune response and inflammation. Conversely, significantly down-regulated proteins included neural cell adhesion molecule, contactin-1, and procollagen C-endopeptidase enhancer, which are involved in neurodevelopment and synaptic plasticity. No differences in serum profiles were observed among the groups. This study identified a panel of CSF protein biomarker candidates for NIME and provided new insights into the pathogenesis of the disease, suggesting that neuronal dysfunction and immune dysregulation may be involved.

犬非传染性脑膜脊髓炎（NIME）的准确死前诊断具有挑战性，因为其临床表现、影像学表现和脑脊液（CSF）分析结果与其他疾病相似。本研究旨在应用最先进的定量蛋白质组学技术来鉴定新的NIME生物标志物。纳入11只狗的血清和脑脊液样本，对照组包括椎间盘疾病患者（IVDD, n=6），研究组包括患有NIME的狗（n=5）。基于质谱的定量蛋白质组学揭示了脑脊液样品中具有显著丰度差异的36种蛋白质。NIME CSF中上调的蛋白包括免疫球蛋白、α -胰蛋白酶抑制剂重链2、酸性鞘磷脂酶样磷酸二酯酶和几丁质酶3样蛋白1，它们都与免疫反应和炎症有关。相反，显著下调的蛋白包括神经细胞粘附分子、接触蛋白-1和前胶原c -内肽酶增强剂，这些蛋白参与神经发育和突触可塑性。各组间血清谱无差异。该研究确定了一组脑脊液蛋白生物标志物候选物，并为该疾病的发病机制提供了新的见解，表明神经元功能障碍和免疫失调可能参与其中。

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引用次数: 0

Effect of patient positioning on retrieval of cystoliths by percutaneous cystolithotomy in dogs 犬经皮膀胱取石术中患者体位对取石效果的影响。

IF 2.3 2区农林科学 Q1 VETERINARY SCIENCES

Veterinary journal

Pub Date : 2025-02-01 DOI: 10.1016/j.tvjl.2024.106291

R.C. Knight , L.J. Owen

Percutaneous cystolithotomy (PCCL) is a minimally-invasive technique for removal of cystoliths. There is currently no information regarding optimum patient positioning during PCCL. The objective of this study was to assess whether patient positioning affected ease of cystolith retrieval via PCCL. PCCL was performed to retrieve artificial “cystoliths” in three canine cadavers weighing between 15 and 35 kg, using a threaded cannula and a rigid 30° 2.7 mm cystoscope. “Cystolith” retrieval was performed by retrograde flushing and use of a flexible stone basket, with the dog in 10° Trendelenburg, 10° reverse Trendelenburg, or neutral position. The location of the “cystoliths”, number retrieved during flushing, and total time for retrieval were recorded and compared between different positions. The mean total time for “cystolith” retrieval was 392 ± 131 s, with 162 ± 20 s for flushing and 221 ± 123 s for basket-retrieval. The mean number of “cystoliths” retrieved during flushing was 3 ± 2. No significant differences were detected when comparing retrieval times between different positions. The predominant location of the “cystoliths” within the bladder varied depending on the position of the dog. In neutral, 93 % were located adjacent to the ureteric openings, whereas in 10° reverse Trendelenburg, 100 % were located by the urethral orifice. In 10° Trendelenburg, 60 % “cystoliths” were located at the tip of the cannula. Cystolith retrieval during PCCL can be performed in either neutral, Trendelenburg or reverse Trendelenburg position. Changing the dog’s position may be useful in cases where cystolith retrieval is challenging, to move the cystoliths and enable different techniques to be employed using the stone basket.

经皮膀胱取石术（PCCL）是一种微创的膀胱取石术。目前还没有关于PCCL患者最佳体位的信息。本研究的目的是评估患者体位是否影响PCCL取膀胱的便利性。在3具体重在15-35kg之间的犬尸体中，使用螺纹插管和30 - 2.7mm硬膀胱镜进行PCCL取人工“膀胱结石”。通过逆行冲洗和使用柔性石筐进行“膀胱结石”回收，犬处于100位、100位逆位或中立位。记录“囊石”的位置、冲洗期间取出的数量和取出的总时间，并比较不同位置之间的差异。“膀胱”取出的平均总时间为392±131s，其中冲洗时间为162±20s，取篮时间为221±123s。冲洗期间取出的“胆囊结石”平均数目为3±2。不同位置的检索次数比较无显著差异。膀胱内“膀胱结石”的主要位置根据犬的位置而变化。中性组93%位于输尿管开口附近，而反向Trendelenburg组100%位于尿道口附近。在100 Trendelenburg中，60%的“胆囊结石”位于套管的尖端。在PCCL期间，可以在中性、Trendelenburg或反Trendelenburg位置进行膀胱结石检索。改变狗的位置可能是有用的，在情况下，膀胱结石检索是具有挑战性的，移动膀胱结石，并使不同的技术使用石篮。

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引用次数: 0

Increased plasma nucleosomes are associated with severe sepsis in foals 血浆核小体增加与马驹严重脓毒症有关。

IF 2.3 2区农林科学 Q1 VETERINARY SCIENCES

Veterinary journal

Pub Date : 2025-02-01 DOI: 10.1016/j.tvjl.2025.106297

E.M. Birckhead, S.L. Raidal, S. Das, S.R. Raidal

Sepsis is a main cause of death in neonatal foals. While the syndrome is not completely understood, sepsis is a dysregulated inflammatory response of the host to infection. It can be difficult to diagnose because of varying and non-specific clinical signs and imperfect diagnostic tests. Increased circulating nucleosome levels have been detected in humans and animals with sepsis, but this has not been documented in foals. Nucleosomes are released extracellularly during neutrophil extracellular trap formation, as well as from damaged and dead cells. We analysed plasma samples from clinically healthy (n = 16), sick non-septic (n = 31) and septic (n = 36) foals using an enzyme-linked immunosorbent assay (ELISA) that targeted nucleosomes. Septic foals with evidence of hypoperfusion and/or organ dysfunction were classified as severe sepsis (n = 24). The main objective was to determine if nucleosome levels were increased in foals with sepsis, particularly those with severe sepsis. Our data identified that nucleosome levels in foals with severe sepsis on the day of study entry were increased significantly compared to all other foals. There was not a significant difference in nucleosome levels between sick non-septic or clinically healthy foals. Foal groups were not age-matched and factors associated with the clinical nature of the study may have affected the results. Further research with larger numbers of foals of similar ages, would be necessary to determine if the analysis of nucleosomes and related biomarkers are helpful adjuncts for the assessment and understanding of equine neonatal sepsis.

败血症是新生儿马驹死亡的主要原因。虽然该综合征尚不完全清楚，但败血症是宿主对感染的炎症反应失调。由于不同的和非特异性的临床体征和不完善的诊断测试，它可能难以诊断。在脓毒症的人类和动物中检测到循环核小体水平升高，但在马驹中没有记录。核小体在中性粒细胞胞外陷阱形成过程中以及从受损和死亡细胞中释放出来。我们使用针对核小体的酶联免疫吸附试验（ELISA）分析了临床健康（n = 16）、患病非脓毒症（n = 31）和脓毒症（n = 36）马驹的血浆样本。有灌注不足和/或器官功能障碍的脓毒症马驹被归类为严重脓毒症（n = 24）。主要目的是确定是否核小体水平增加的马驹败血症，特别是那些严重败血症。我们的数据表明，与所有其他马驹相比，在研究进入当天患有严重败血症的马驹的核小体水平显着增加。患病的非败血症马驹和临床健康马驹之间的核小体水平没有显著差异。马驹组的年龄不匹配，与研究的临床性质相关的因素可能影响了结果。为了确定核小体和相关生物标志物的分析是否有助于评估和了解马新生儿败血症，有必要对大量相近年龄的马驹进行进一步的研究。

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