Validation of the Lead Care II System in Cape vultures (Gyps coprotheres) in comparison to ICP-MS using pure standards

IF 4.2 3区环境科学与生态学 Q2 ENVIRONMENTAL SCIENCES Environmental toxicology and pharmacology Pub Date : 2024-08-06 DOI:10.1016/j.etap.2024.104530

V. Naidoo , K. Wolter

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Abstract

Lead toxicosis remains a concern in raptors, especially following feeding on carcasses sourced from hunting. Rapid diagnosis of lead exposure and easy field monitoring is desirable. The LeadCareII analytical system, validated for rapid diagnoses of lead toxicity in humans, has been described as a useful evaluation system in various species. For this study we attempt to validate the LeadCareII system in the Cape Vulture (CV) (Gyps coprotheres). Blood samples from CV housed under captive conditions and low background lead exposure, were pooled and spiked with known concentrations of a lead standard (0–60 µg/dL). Samples were analyzed by the LeadCareII system and by ICP-MS. The final results showed that despite good linearity the LeadCareII system underestimated lead concentrations by up to 50 %. While the results can be corrected by the derived equation, this is not supported due to the large underestimations evident. The reason for the underestimation is presently unknown.

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与使用纯标准物质的 ICP-MS 相比，验证开普秃鹫（Gyps coprotheres）体内的铅 Care II 系统。

铅中毒仍然是猛禽关注的问题，尤其是在捕食猎物尸体后。对铅暴露的快速诊断和简便的现场监测是可取的。经过验证，LeadCareII 分析系统可用于快速诊断人类的铅中毒，该系统已被描述为适用于各种物种的评估系统。在这项研究中，我们尝试在秃鹫（CV）（Gyps coprotheres）身上验证 LeadCareII 系统。在圈养条件下饲养的开普秃鹫（CV）的血液样本本底铅暴露量较低，我们将这些样本集中起来，并在其中添加已知浓度的铅标准物质（0 至 60µg/dL ）。样品由 LeadCareII 系统和 ICP-MS 进行分析。最终结果显示，尽管线性度良好，LeadCareII 系统还是低估了高达 50% 的铅浓度。虽然可以通过推导出的方程对结果进行修正，但由于明显的大幅低估，这一方法并不被支持。低估的原因目前尚不清楚。

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来源期刊

Environmental toxicology and pharmacology 医学-毒理学

CiteScore

7.00

自引率

4.70%

发文量

185

审稿时长

34 days

期刊介绍： Environmental Toxicology and Pharmacology publishes the results of studies concerning toxic and pharmacological effects of (human and veterinary) drugs and of environmental contaminants in animals and man. Areas of special interest are: molecular mechanisms of toxicity, biotransformation and toxicokinetics (including toxicokinetic modelling), molecular, biochemical and physiological mechanisms explaining differences in sensitivity between species and individuals, the characterisation of pathophysiological models and mechanisms involved in the development of effects and the identification of biological markers that can be used to study exposure and effects in man and animals. In addition to full length papers, short communications, full-length reviews and mini-reviews, Environmental Toxicology and Pharmacology will publish in depth assessments of special problem areas. The latter publications may exceed the length of a full length paper three to fourfold. A basic requirement is that the assessments are made under the auspices of international groups of leading experts in the fields concerned. The information examined may either consist of data that were already published, or of new data that were obtained within the framework of collaborative research programmes. Provision is also made for the acceptance of minireviews on (classes of) compounds, toxicities or mechanisms, debating recent advances in rapidly developing fields that fall within the scope of the journal.