Pub Date : 2025-12-19DOI: 10.1016/j.etap.2025.104907
Xia Wang, Ruixuan Li, Bo Cheng, Yingxue Sun, Xiaolong Yao, Chun Wang
{"title":"Combined toxicity of polyethylene micro/nanoplastics and PFOA in zebrafish (Danio rerio): Impacts on antioxidant, neurotransmission, and gut microbiota","authors":"Xia Wang, Ruixuan Li, Bo Cheng, Yingxue Sun, Xiaolong Yao, Chun Wang","doi":"10.1016/j.etap.2025.104907","DOIUrl":"https://doi.org/10.1016/j.etap.2025.104907","url":null,"abstract":"","PeriodicalId":11775,"journal":{"name":"Environmental toxicology and pharmacology","volume":"11 1","pages":""},"PeriodicalIF":4.3,"publicationDate":"2025-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145785202","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-12-19DOI: 10.1016/j.etap.2025.104914
Nivedita Jha, Angshita Das, Megha Prasanth, S Daisy Precilla, R Sivachandran, Rajaneesh Anupam, T.S Anitha
{"title":"Smoke-Derived Microplastics of Polypropylene In Vitro Proliferative Impact on Human Lung Adenocarcinoma Cells: Physicochemical Analysis and Biological Evaluation","authors":"Nivedita Jha, Angshita Das, Megha Prasanth, S Daisy Precilla, R Sivachandran, Rajaneesh Anupam, T.S Anitha","doi":"10.1016/j.etap.2025.104914","DOIUrl":"https://doi.org/10.1016/j.etap.2025.104914","url":null,"abstract":"","PeriodicalId":11775,"journal":{"name":"Environmental toxicology and pharmacology","volume":"27 1","pages":""},"PeriodicalIF":4.3,"publicationDate":"2025-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145785203","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-12-18DOI: 10.1016/j.etap.2025.104915
Bozhena Tsyupa, Chiara Emma Campiglio, Elena Carrara, Marta Ripamonti, Alessandro Mancini, Andrea Bonfanti, Micol Introna, Ana Teresa Juarez-Facio, Karine Elihn, Manuela Teresa Raimondi, Andrea Remuzzi
{"title":"On-site ALI versus Submerged Culture: Chemical and Toxicological Investigation of Brake Wear Sub-micrometric Particles","authors":"Bozhena Tsyupa, Chiara Emma Campiglio, Elena Carrara, Marta Ripamonti, Alessandro Mancini, Andrea Bonfanti, Micol Introna, Ana Teresa Juarez-Facio, Karine Elihn, Manuela Teresa Raimondi, Andrea Remuzzi","doi":"10.1016/j.etap.2025.104915","DOIUrl":"https://doi.org/10.1016/j.etap.2025.104915","url":null,"abstract":"","PeriodicalId":11775,"journal":{"name":"Environmental toxicology and pharmacology","volume":"40 1","pages":""},"PeriodicalIF":4.3,"publicationDate":"2025-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145785206","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-12-17DOI: 10.1016/j.etap.2025.104910
Natália P.V.M. Faria , Amanda da S. Guerreiro , Giovana S. Branco , Mariana F. de Campos , Renata G. Moreira , Ismael E. Lozano , Renato M. Honji
Phenanthrene (PHE), a polycyclic aromatic hydrocarbon (PAH), poses significant risks to marine biota, yet its behavioral and physiological effects on fish, particularly on Epinephelus marginatus, remain poorly understood. This study therefore aimed to assess the acute (96 h) effects of PHE exposure (0.1 and 1 mg L⁻¹) on juvenile E. marginatus (n = 15 per treatment) compared to an ethanol control (0.004 %). Behavioral endpoints, oxidative stress biomarkers, plasma cortisol levels, and acetylcholinesterase activity were evaluated in the same individuals. PHE-exposed fish exhibited pronounced behavioral disturbances, including reduced agonistic interactions and loss of equilibrium at the highest concentration, which may compromise social hierarchy and predator avoidance. Although acetylcholinesterase and antioxidant enzymes remained unchanged, the higher PHE concentration triggered increased plasma cortisol and hepatic lipid peroxidation, indicating stress axis activation and oxidative damage. These results provide the first integrated evidence of neurobehavioral and physiological disruption in E. marginatus under PHE exposure.
{"title":"Impacts of phenanthrene on juveniles of dusky grouper, Epinephelus marginatus (Perciformes: Serranidae): Behavioral, endocrine and oxidative effects","authors":"Natália P.V.M. Faria , Amanda da S. Guerreiro , Giovana S. Branco , Mariana F. de Campos , Renata G. Moreira , Ismael E. Lozano , Renato M. Honji","doi":"10.1016/j.etap.2025.104910","DOIUrl":"10.1016/j.etap.2025.104910","url":null,"abstract":"<div><div>Phenanthrene (PHE), a polycyclic aromatic hydrocarbon (PAH), poses significant risks to marine biota, yet its behavioral and physiological effects on fish, particularly on <em>Epinephelus marginatus</em>, remain poorly understood. This study therefore aimed to assess the acute (96 h) effects of PHE exposure (0.1 and 1 mg L⁻¹) on juvenile <em>E. marginatus</em> (n = 15 per treatment) compared to an ethanol control (0.004 %). Behavioral endpoints, oxidative stress biomarkers, plasma cortisol levels, and acetylcholinesterase activity were evaluated in the same individuals. PHE-exposed fish exhibited pronounced behavioral disturbances, including reduced agonistic interactions and loss of equilibrium at the highest concentration, which may compromise social hierarchy and predator avoidance. Although acetylcholinesterase and antioxidant enzymes remained unchanged, the higher PHE concentration triggered increased plasma cortisol and hepatic lipid peroxidation, indicating stress axis activation and oxidative damage. These results provide the first integrated evidence of neurobehavioral and physiological disruption in <em>E. marginatus</em> under PHE exposure.</div></div>","PeriodicalId":11775,"journal":{"name":"Environmental toxicology and pharmacology","volume":"121 ","pages":"Article 104910"},"PeriodicalIF":4.2,"publicationDate":"2025-12-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145785207","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-12-16DOI: 10.1016/j.etap.2025.104913
Martin Staron , Ľubica Rajčáková , Matej Planý , Hossam Abou-Shaara
Nutrition strongly shapes honey bee resilience, especially when bees face multiple environmental stressors. This study examined how different carbohydrate and commercial protein diets influence honey bee responses to residue-level tebuconazole exposure and short-term heat stress (40°C for 48 h). Mortality, food intake, and body water loss were measured under each treatment. Diet had a significant effect on survival: among carbohydrate diets, bees fed the control diet showed the lowest mortality, whereas rapeseed honey caused the highest. For protein diets, sugar candy and the vegetable protein diet yielded the highest survival, in contrast to the pollen-based feed. Food intake varied widely across diets and showed a positive association with mortality. The presence of tebuconazole in diets did not induce avoidance or reduce consumption. Water loss was inversely associated with mortality, suggesting complex physiological trade-offs. Tebuconazole did not cause acute lethal effects, contributing to current evaluations of fungicide risks.
{"title":"Interactive effects of diet, tebuconazole, and elevated temperature on survival and body water loss in honey bee workers","authors":"Martin Staron , Ľubica Rajčáková , Matej Planý , Hossam Abou-Shaara","doi":"10.1016/j.etap.2025.104913","DOIUrl":"10.1016/j.etap.2025.104913","url":null,"abstract":"<div><div>Nutrition strongly shapes honey bee resilience, especially when bees face multiple environmental stressors. This study examined how different carbohydrate and commercial protein diets influence honey bee responses to residue-level tebuconazole exposure and short-term heat stress (40°C for 48 h). Mortality, food intake, and body water loss were measured under each treatment. Diet had a significant effect on survival: among carbohydrate diets, bees fed the control diet showed the lowest mortality, whereas rapeseed honey caused the highest. For protein diets, sugar candy and the vegetable protein diet yielded the highest survival, in contrast to the pollen-based feed. Food intake varied widely across diets and showed a positive association with mortality. The presence of tebuconazole in diets did not induce avoidance or reduce consumption. Water loss was inversely associated with mortality, suggesting complex physiological trade-offs. Tebuconazole did not cause acute lethal effects, contributing to current evaluations of fungicide risks.</div></div>","PeriodicalId":11775,"journal":{"name":"Environmental toxicology and pharmacology","volume":"121 ","pages":"Article 104913"},"PeriodicalIF":4.2,"publicationDate":"2025-12-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145784054","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-12-15DOI: 10.1016/j.etap.2025.104908
Lin Tao , Lulu Dai , Shimin Xiong , Dengqing Liao , Yuan-zhong Zhou , Xubo Shen
Background
This study aimed to comprehensively investigate the associations among maternal immune-inflammatory markers and urinary phthalate (PAE) metabolites during pregnancy, focusing on their potential links with preeclampsia and related reproductive outcomes.
Method
A propensity score-matched case-control design was adopted, enrolling 61 cases and 118 controls matched based on propensity scores. To assess associations, dose-response relationships, and mediating effects, multiple statistical methods were employed, including logistic regression, restricted cubic splines (RCS), Bayesian kernel machine regression (BKMR), and structural equation modeling (SEM).
Results
Maternal urinary metabolites of PAEs (MEHP, MEHHP) and the systemic immune-inflammation index (SII) were significantly higher in the case group than in the control group. Logistic regression analysis revealed positive associations between MEHP, MOP, MEHHP, SII, and preeclampsia (all odds ratios [OR] > 1, P < 0.05). RCS analysis revealed nonlinear dose-response relationships for MOP and SII, with threshold concentrations of 2.57 μg/L creatinine (MOP) and 977.24 μg/L (SII). BKMR results indicated a nonlinear positive correlation between PAE metabolites, immune-inflammatory markers, and spontaneous abortion. SEM models confirmed mediating effects of immune-inflammatory markers: SII mediated the associations between MEHP, MOP, MEHHP and preeclampsia with mediation rates (95 %CI) of 8.73 % [1.33 %–22.34 %], 25.31 % [11.72 %–36.00 %], and 6.50 % [0.36 %–21.39 %], respectively; AISI mediated the MEHP-preeclampsia association (0.27 % [0.20 %–1.19 %]); and MLR and PLR mediated the MEHHP-preeclampsia association (2.54 % [1.53 %–8.80 %] and 7.59 % [1.20 %–12.52 %], respectively).
Conclusion
Maternal urinary PAE metabolites during pregnancy are associated with an increased risk of preeclampsia, and maternal immune-inflammatory markers partially mediate this relationship. These findings provide insights into the potential mechanisms linking prenatal PAE exposure to adverse pregnancy outcomes.
{"title":"Maternal immune-inflammatory markers mediate the association between urinary phthalate metabolites and preeclampsia","authors":"Lin Tao , Lulu Dai , Shimin Xiong , Dengqing Liao , Yuan-zhong Zhou , Xubo Shen","doi":"10.1016/j.etap.2025.104908","DOIUrl":"10.1016/j.etap.2025.104908","url":null,"abstract":"<div><h3>Background</h3><div>This study aimed to comprehensively investigate the associations among maternal immune-inflammatory markers and urinary phthalate (PAE) metabolites during pregnancy, focusing on their potential links with preeclampsia and related reproductive outcomes.</div></div><div><h3>Method</h3><div>A propensity score-matched case-control design was adopted, enrolling 61 cases and 118 controls matched based on propensity scores. To assess associations, dose-response relationships, and mediating effects, multiple statistical methods were employed, including logistic regression, restricted cubic splines (RCS), Bayesian kernel machine regression (BKMR), and structural equation modeling (SEM).</div></div><div><h3>Results</h3><div>Maternal urinary metabolites of PAEs (MEHP, MEHHP) and the systemic immune-inflammation index (SII) were significantly higher in the case group than in the control group. Logistic regression analysis revealed positive associations between MEHP, MOP, MEHHP, SII, and preeclampsia (all odds ratios [OR] > 1, P < 0.05). RCS analysis revealed nonlinear dose-response relationships for MOP and SII, with threshold concentrations of 2.57 μg/L creatinine (MOP) and 977.24 μg/L (SII). BKMR results indicated a nonlinear positive correlation between PAE metabolites, immune-inflammatory markers, and spontaneous abortion. SEM models confirmed mediating effects of immune-inflammatory markers: SII mediated the associations between MEHP, MOP, MEHHP and preeclampsia with mediation rates (95 %CI) of 8.73 % [1.33 %–22.34 %], 25.31 % [11.72 %–36.00 %], and 6.50 % [0.36 %–21.39 %], respectively; AISI mediated the MEHP-preeclampsia association (0.27 % [0.20 %–1.19 %]); and MLR and PLR mediated the MEHHP-preeclampsia association (2.54 % [1.53 %–8.80 %] and 7.59 % [1.20 %–12.52 %], respectively).</div></div><div><h3>Conclusion</h3><div>Maternal urinary PAE metabolites during pregnancy are associated with an increased risk of preeclampsia, and maternal immune-inflammatory markers partially mediate this relationship. These findings provide insights into the potential mechanisms linking prenatal PAE exposure to adverse pregnancy outcomes.</div></div>","PeriodicalId":11775,"journal":{"name":"Environmental toxicology and pharmacology","volume":"121 ","pages":"Article 104908"},"PeriodicalIF":4.2,"publicationDate":"2025-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145776873","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-12-15DOI: 10.1016/j.etap.2025.104911
Phuoc-Cuong Le , Le Van Hao , Tan Nhat , Hoang-Son Le , Thi Xuan Thuy Le
This study assesses seasonal heavy-metal contamination from gold mining in the Bong Mieu River basin (central Viet Nam) and examines its association with human xenobiotic-metabolizing enzyme phenotypes. In sediments, As and Pb consistently exceeded Vietnamese sediment quality limits, by 4.1–25.4 × for As and 2.3–7.8 × for Pb, whereas other metals generally remained within regulatory thresholds. River water exhibited widespread Pb exceedances at all sites and occasional. As exceedances, with higher concentrations in the dry season. Regression analyses indicated strong water–sediment coupling for As, Pb, Hg, Cd, and Zn, supporting shared transport and accumulation processes. Season-resolved Pearson correlations, HCA, PCA, and APCS-MLR source apportionment revealed a dominant mining-derived multi-metal signal year-round, intensified and spatially mixed under monsoon hydrology. Enzyme phenotypes varied with the mixed-metal exposure gradient: sites with higher As/Pb burdens showed higher frequencies of slow NAT2 and intermediate/slow CYP450 phenotypes, and longer residence was associated with higher NAT2 rapid-phenotype frequency.
{"title":"Arsenic and lead exposure in vietnamese gold mining communities: A case study of metabolic enzyme impacts","authors":"Phuoc-Cuong Le , Le Van Hao , Tan Nhat , Hoang-Son Le , Thi Xuan Thuy Le","doi":"10.1016/j.etap.2025.104911","DOIUrl":"10.1016/j.etap.2025.104911","url":null,"abstract":"<div><div>This study assesses seasonal heavy-metal contamination from gold mining in the Bong Mieu River basin (central Viet Nam) and examines its association with human xenobiotic-metabolizing enzyme phenotypes. In sediments, As and Pb consistently exceeded Vietnamese sediment quality limits, by 4.1–25.4 × for As and 2.3–7.8 × for Pb, whereas other metals generally remained within regulatory thresholds. River water exhibited widespread Pb exceedances at all sites and occasional. As exceedances, with higher concentrations in the dry season. Regression analyses indicated strong water–sediment coupling for As, Pb, Hg, Cd, and Zn, supporting shared transport and accumulation processes. Season-resolved Pearson correlations, HCA, PCA, and APCS-MLR source apportionment revealed a dominant mining-derived multi-metal signal year-round, intensified and spatially mixed under monsoon hydrology. Enzyme phenotypes varied with the mixed-metal exposure gradient: sites with higher As/Pb burdens showed higher frequencies of slow NAT2 and intermediate/slow CYP450 phenotypes, and longer residence was associated with higher NAT2 rapid-phenotype frequency.</div></div>","PeriodicalId":11775,"journal":{"name":"Environmental toxicology and pharmacology","volume":"121 ","pages":"Article 104911"},"PeriodicalIF":4.2,"publicationDate":"2025-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145776925","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-12-15DOI: 10.1016/j.etap.2025.104912
Alice Gabetti , Camilla Mossotto , Francesca Provenza , Serena Anselmi , Alessandra Maganza , Giuseppe Esposito , Maria Vittoria Riina , Alessandra Griglione , Stefania Squadrone , Monia Renzi , Antonia Concetta Elia , Marino Prearo , Paolo Pastorino
The increasing anthropogenic release of gadolinium (Gd) from medical contrast agents raises concern regarding its fate and ecotoxicological effects in freshwater ecosystems. Gd may reach alpine lakes through atmospheric deposition, entering environments already fragile due to extreme conditions. This study examined the sublethal effects of Gd on the alpine copepod Cyclops abyssorum under simulated winter conditions (4 °C, darkness). Organisms were exposed for 14 days to 1–150 µg/L Gd, and oxidative stress biomarkers were measured. Glutathione peroxidase activity increased with concentration, while malondialdehyde indicated lipid peroxidation. Superoxide dismutase and glutathione S-transferase showed non-linear responses, suggesting physiological thresholds or enzyme inhibition. The Threshold-based Integrative Biomarker Response (IBR-T) index revealed progressively higher stress at intermediate and high exposures. Overall, the results indicate that Gd can induce oxidative stress in cold-adapted zooplankton, emphasizing the importance of assessing contaminant risks in alpine lakes using non-model species and realistic exposure scenarios.
{"title":"What lies beneath the ice? How gadolinium affects Cyclops abyssorum during Alpine winters","authors":"Alice Gabetti , Camilla Mossotto , Francesca Provenza , Serena Anselmi , Alessandra Maganza , Giuseppe Esposito , Maria Vittoria Riina , Alessandra Griglione , Stefania Squadrone , Monia Renzi , Antonia Concetta Elia , Marino Prearo , Paolo Pastorino","doi":"10.1016/j.etap.2025.104912","DOIUrl":"10.1016/j.etap.2025.104912","url":null,"abstract":"<div><div>The increasing anthropogenic release of gadolinium (Gd) from medical contrast agents raises concern regarding its fate and ecotoxicological effects in freshwater ecosystems. Gd may reach alpine lakes through atmospheric deposition, entering environments already fragile due to extreme conditions. This study examined the sublethal effects of Gd on the alpine copepod <em>Cyclops abyssorum</em> under simulated winter conditions (4 °C, darkness). Organisms were exposed for 14 days to 1–150 µg/L Gd, and oxidative stress biomarkers were measured. Glutathione peroxidase activity increased with concentration, while malondialdehyde indicated lipid peroxidation. Superoxide dismutase and glutathione S-transferase showed non-linear responses, suggesting physiological thresholds or enzyme inhibition. The Threshold-based Integrative Biomarker Response (IBR-T) index revealed progressively higher stress at intermediate and high exposures. Overall, the results indicate that Gd can induce oxidative stress in cold-adapted zooplankton, emphasizing the importance of assessing contaminant risks in alpine lakes using non-model species and realistic exposure scenarios.</div></div>","PeriodicalId":11775,"journal":{"name":"Environmental toxicology and pharmacology","volume":"121 ","pages":"Article 104912"},"PeriodicalIF":4.2,"publicationDate":"2025-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145776899","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-12-12DOI: 10.1016/j.etap.2025.104909
Ryan Brownlee , Maria E.A. Galiciolli , Lucy Joshee , Adam M. Kiefer , Caryn S. Seney , Christy C. Bridges
The processing of gold ore with both mercury (Hg) and cyanide (-CN) leads to widespread environmental contamination. Hg and its related compounds form complexes with -CN in the environment, which may result in human exposure through ingestion of contaminated food or water. The kidney is a significant site of Hg accumulation, but the mechanisms by which mercuric cyanide (Hg(CN)n2-n) and methylmercuric cyanide (H3CHg(CN)n1-n) complexes are taken up by renal tubular cells are unknown. We hypothesized that the organic anion transporter (OAT1) is involved in the uptake of these complexes. To test this hypothesis, the time course, temperature-dependence, saturation kinetics, and substrate specificity of Hg uptake were examined in human embryonic kidney cells stably transfected with OAT1. Our data show that these complexes are taken into proximal tubular cells via OAT1. These data are the first to identify a mechanism involved in the uptake of mercuric-cyanide species into proximal tubular cells.
{"title":"Organic anion transporter 1 (OAT1) is a mechanism for uptake of mercuric-cyanide complexes into proximal tubular cells","authors":"Ryan Brownlee , Maria E.A. Galiciolli , Lucy Joshee , Adam M. Kiefer , Caryn S. Seney , Christy C. Bridges","doi":"10.1016/j.etap.2025.104909","DOIUrl":"10.1016/j.etap.2025.104909","url":null,"abstract":"<div><div>The processing of gold ore with both mercury (Hg) and cyanide (<sup>-</sup>CN) leads to widespread environmental contamination. Hg and its related compounds form complexes with <sup>-</sup>CN in the environment, which may result in human exposure through ingestion of contaminated food or water. The kidney is a significant site of Hg accumulation, but the mechanisms by which mercuric cyanide (Hg(CN)<sub>n</sub><sup>2-n</sup>) and methylmercuric cyanide (H<sub>3</sub>CHg(CN)<sub>n</sub><sup>1-n</sup>) complexes are taken up by renal tubular cells are unknown. We hypothesized that the organic anion transporter (OAT1) is involved in the uptake of these complexes. To test this hypothesis, the time course, temperature-dependence, saturation kinetics, and substrate specificity of Hg uptake were examined in human embryonic kidney cells stably transfected with OAT1. Our data show that these complexes are taken into proximal tubular cells via OAT1. These data are the first to identify a mechanism involved in the uptake of mercuric-cyanide species into proximal tubular cells.</div></div>","PeriodicalId":11775,"journal":{"name":"Environmental toxicology and pharmacology","volume":"121 ","pages":"Article 104909"},"PeriodicalIF":4.2,"publicationDate":"2025-12-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145731818","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-12-11DOI: 10.1016/j.etap.2025.104903
Ana Caroline Pimentel de Oliveira , Letícia Ramos Molica , Karen C.M. Moraes
Pesticides are used worldwide and cause several pathologies as metabolic dysfunction-associated steatotic liver disease. Steatosis is a mark of the disease beginning; however, the mechanisms by which pesticides act on this dysfunction require further understanding. Thus, this work presents a sensitive and responsive low-cost hepatic multicellular spheroid useful to detail metabolic routes in hepatic dysfunction. Using those spheroids, the effects of pesticides bromacil and terbacil were assessed. Pesticides doses near the acceptable daily intake in Brazil changes energetic, oxidative and the one-carbon metabolism. Moreover, pesticide-specific effects were observed as increased expression of FASN, PPARs, CYP1A1, AHR, DNMTs among others, and changes in the methylation pattern of DNA, RNA and histones. Combined, the results were alarming, considering the long-lasting effects of the evaluated pesticides in agriculture fields. In addition, we can suggest that our spheroid model could be useful for large scale investigation of the effects of pesticides in liver metabolism.
{"title":"Assessing the effects of uracil-based pesticides on hepatic metabolic dysfunctions in low-cost three-dimensional multicellular spheroids","authors":"Ana Caroline Pimentel de Oliveira , Letícia Ramos Molica , Karen C.M. Moraes","doi":"10.1016/j.etap.2025.104903","DOIUrl":"10.1016/j.etap.2025.104903","url":null,"abstract":"<div><div>Pesticides are used worldwide and cause several pathologies as metabolic dysfunction-associated steatotic liver disease. Steatosis is a mark of the disease beginning; however, the mechanisms by which pesticides act on this dysfunction require further understanding. Thus, this work presents a sensitive and responsive low-cost hepatic multicellular spheroid useful to detail metabolic routes in hepatic dysfunction. Using those spheroids, the effects of pesticides bromacil and terbacil were assessed. Pesticides doses near the acceptable daily intake in Brazil changes energetic, oxidative and the one-carbon metabolism. Moreover, pesticide-specific effects were observed as increased expression of <em>FASN</em>, <em>PPAR</em>s, <em>CYP1A1</em>, <em>AHR</em>, <em>DNMT</em>s among others, and changes in the methylation pattern of DNA, RNA and histones. Combined, the results were alarming, considering the long-lasting effects of the evaluated pesticides in agriculture fields. In addition, we can suggest that our spheroid model could be useful for large scale investigation of the effects of pesticides in liver metabolism.</div></div>","PeriodicalId":11775,"journal":{"name":"Environmental toxicology and pharmacology","volume":"121 ","pages":"Article 104903"},"PeriodicalIF":4.2,"publicationDate":"2025-12-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145731817","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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