Environmental toxicology and pharmacology最新文献

Environmental exposure to pesticides is associated with oxidative stress, oxidative DNA damage, and elevated interleukin-8 in a child population.

IF 4.2 3区环境科学与生态学 Q2 ENVIRONMENTAL SCIENCES

Environmental toxicology and pharmacology

Pub Date : 2025-02-18 DOI: 10.1016/j.etap.2025.104656

José Antonio Aguilar-Bañuelos , Yael Yvette Bernal-Hernández , Irma Martha Medina-Díaz , Miguel Alfonso Ruiz-Arias , José Francisco Herrera-Moreno , Briscia Socorro Barrón-Vivanco , Cyndia Azucena González-Arias , Juan Manuel Agraz-Cibrián , José Francisco Zambrano-Zaragoza , Francisco Alberto Verdín-Betancourt , Néstor Ponce Ruiz , Eugenia Flores-Alfaro , Aurora Elizabeth Rojas-García

Pesticide exposure can cause various adverse effects in humans, with children being particularly susceptible. Such exposure leads to neurological, immunological, respiratory, and genetic damage, primarily by generating reactive oxygen species (ROS). The increase in ROS induces lipid peroxidation (LPO) and the formation of hydroxyl radicals, which generate DNA adducts. This study involved children aged 6–12 from three communities: two in an agricultural region (communities A and B) and one reference population (community C). The objective was to evaluate lipid peroxidation through malondialdehyde (MDA) levels, the content of 8-hydroxy-2′-deoxyguanosine (8-OHdG) adducts, and the concentrations of the cytokines IL-6, IL-8, IL-10, and TNF-α in children environmentally exposed to pesticides. Anthropometric measurements were taken from the study population. Dialkylphosphates (DAP) in urine were determined by gas chromatography and mass spectrometry. Plasma concentrations of MDA and pro-inflammatory cytokines (IL-6, IL-8, TNF-α) and the anti-inflammatory cytokine (IL-10) were quantified using biochemical assays and urinary concentrations of 8-OHdG. The findings showed that DAP, MDA, and 8-OHdG concentrations in communities A and B increased significantly compared with community C. Additionally, IL-8 exhibited a significant increase in community A compared to community C, while no significant differences were observed for IL-6, IL-10, and TNF-α. Higher pesticide exposure is linked to oxidative stress, DNA damage and inflammation, key indicators of chronic diseases. In conclusion, this study provides evidence linking environmental pesticide exposure in agricultural communities to increased oxidative stress and inflammatory responses in children.

{"title":"Environmental exposure to pesticides is associated with oxidative stress, oxidative DNA damage, and elevated interleukin-8 in a child population.","authors":"José Antonio Aguilar-Bañuelos , Yael Yvette Bernal-Hernández , Irma Martha Medina-Díaz , Miguel Alfonso Ruiz-Arias , José Francisco Herrera-Moreno , Briscia Socorro Barrón-Vivanco , Cyndia Azucena González-Arias , Juan Manuel Agraz-Cibrián , José Francisco Zambrano-Zaragoza , Francisco Alberto Verdín-Betancourt , Néstor Ponce Ruiz , Eugenia Flores-Alfaro , Aurora Elizabeth Rojas-García","doi":"10.1016/j.etap.2025.104656","DOIUrl":"10.1016/j.etap.2025.104656","url":null,"abstract":"<div><div>Pesticide exposure can cause various adverse effects in humans, with children being particularly susceptible. Such exposure leads to neurological, immunological, respiratory, and genetic damage, primarily by generating reactive oxygen species (ROS). The increase in ROS induces lipid peroxidation (LPO) and the formation of hydroxyl radicals, which generate DNA adducts. This study involved children aged 6–12 from three communities: two in an agricultural region (communities A and B) and one reference population (community C). The objective was to evaluate lipid peroxidation through malondialdehyde (MDA) levels, the content of 8-hydroxy-2′-deoxyguanosine (8-OHdG) adducts, and the concentrations of the cytokines IL-6, IL-8, IL-10, and TNF-α in children environmentally exposed to pesticides. Anthropometric measurements were taken from the study population. Dialkylphosphates (DAP) in urine were determined by gas chromatography and mass spectrometry. Plasma concentrations of MDA and pro-inflammatory cytokines (IL-6, IL-8, TNF-α) and the anti-inflammatory cytokine (IL-10) were quantified using biochemical assays and urinary concentrations of 8-OHdG. The findings showed that DAP, MDA, and 8-OHdG concentrations in communities A and B increased significantly compared with community C. Additionally, IL-8 exhibited a significant increase in community A compared to community C, while no significant differences were observed for IL-6, IL-10, and TNF-α. Higher pesticide exposure is linked to oxidative stress, DNA damage and inflammation, key indicators of chronic diseases. In conclusion, this study provides evidence linking environmental pesticide exposure in agricultural communities to increased oxidative stress and inflammatory responses in children.</div></div>","PeriodicalId":11775,"journal":{"name":"Environmental toxicology and pharmacology","volume":"114 ","pages":"Article 104656"},"PeriodicalIF":4.2,"publicationDate":"2025-02-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143444352","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Platelet and IL-33 count as biomarkers for lung function impairment: An 11-year follow-up study on populations exposed to hexavalent chromium

IF 4.2 3区环境科学与生态学 Q2 ENVIRONMENTAL SCIENCES

Environmental toxicology and pharmacology

Pub Date : 2025-02-18 DOI: 10.1016/j.etap.2025.104660

Pai Zheng , Yi Zhang , Jiaqi Shi , Zekang Su , Guiping Hu , Yi Bai , Zhangjian Chen , Guang Jia

Recent research has highlighted the crucial role of immune regulation in lung function impairment due to exposure to hazardous materials. This study aimed to identify dynamic network biomarkers for lung function damage caused by hexavalent chromium inhalation exposure, using immune-related indicators in blood. An 11-year follow-up longitudinal study was conducted on a population occupationally exposed to hexavalent chromate (Cr [VI]) from 2010 to 2020, consisting of sixty-one subjects with 328 repeat measurements. Quantitative analysis of immune-related indicators, including white blood cells, cytokines, and platelet count, was performed. The concentration of urinary Cr served as an indicator of internal exposure, confirming its association with lung function impairment. Dynamic network analysis revealed that platelet count was connected to neutrophils, IL-8, IL-6, and IL-33 when the exposure time was equal to or longer than 9.2 years (the median exposure time). Notably, the association between platelet count and IL-33 was specific to long-term (≥ 9.2 years) exposure. The areas under the receiver operating characteristic (ROC) curves (AUCs) for platelet count combined with IL-33 to predict lung function impairment in the long-term and short-term Cr [VI]-exposed populations were 80.5 % and 55.4 %, respectively. These findings provide evidence that the combination of platelets and IL-33 holds significant promise as biomarkers for predicting lung function impairment. Moreover, they shed light on the potential mechanism involving immune and hematopoiesis functions in the context of environmental hazardous exposure.

{"title":"Platelet and IL-33 count as biomarkers for lung function impairment: An 11-year follow-up study on populations exposed to hexavalent chromium","authors":"Pai Zheng , Yi Zhang , Jiaqi Shi , Zekang Su , Guiping Hu , Yi Bai , Zhangjian Chen , Guang Jia","doi":"10.1016/j.etap.2025.104660","DOIUrl":"10.1016/j.etap.2025.104660","url":null,"abstract":"<div><div>Recent research has highlighted the crucial role of immune regulation in lung function impairment due to exposure to hazardous materials. This study aimed to identify dynamic network biomarkers for lung function damage caused by hexavalent chromium inhalation exposure, using immune-related indicators in blood. An 11-year follow-up longitudinal study was conducted on a population occupationally exposed to hexavalent chromate (Cr [VI]) from 2010 to 2020, consisting of sixty-one subjects with 328 repeat measurements. Quantitative analysis of immune-related indicators, including white blood cells, cytokines, and platelet count, was performed. The concentration of urinary Cr served as an indicator of internal exposure, confirming its association with lung function impairment. Dynamic network analysis revealed that platelet count was connected to neutrophils, IL-8, IL-6, and IL-33 when the exposure time was equal to or longer than 9.2 years (the median exposure time). Notably, the association between platelet count and IL-33 was specific to long-term (≥ 9.2 years) exposure. The areas under the receiver operating characteristic (ROC) curves (AUCs) for platelet count combined with IL-33 to predict lung function impairment in the long-term and short-term Cr [VI]-exposed populations were 80.5 % and 55.4 %, respectively. These findings provide evidence that the combination of platelets and IL-33 holds significant promise as biomarkers for predicting lung function impairment. Moreover, they shed light on the potential mechanism involving immune and hematopoiesis functions in the context of environmental hazardous exposure.</div></div>","PeriodicalId":11775,"journal":{"name":"Environmental toxicology and pharmacology","volume":"114 ","pages":"Article 104660"},"PeriodicalIF":4.2,"publicationDate":"2025-02-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143444351","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Toxic effects of microplastics (polyethylene) exposure on acetylcholinesterase, stress indicators and immunity in Korean Bullhead, Pseudobagrus fulvidraco

IF 4.2 3区环境科学与生态学 Q2 ENVIRONMENTAL SCIENCES

Environmental toxicology and pharmacology

Pub Date : 2025-02-11 DOI: 10.1016/j.etap.2025.104654

Ju-Hyeong Lee , Jae-Ho Choi , Yue Jai Kang , Young Jae Choi , Cheol Young Choi , Ju-Chan Kang , Jun-Hwan Kim

Microplastics (MPs) in aquatic environments can have toxic effects on various organisms, including fish. This study exposed Pseudobagrus fulvidraco to polyethylene MPs at 0, 10 mg/L (approximately 9.50 ×10⁸ particles/L), 20 mg/L (approximately 1.9 ×10⁹ particles/L), 5000 mg/L (approximately 4.75 ×10¹¹ particles/L), and 10,000 mg/L (approximately 9.50 ×10¹¹ particles/L) concentrations for 96 h. At relatively lower MPs concentrations (0, 10 and 20 mg/L), no significant changes were observed in acetylcholinesterase (AChE) activity, stress indicators (heat shock protein 70 and cortisol), or immune responses (lysozyme activity and immunoglobulin M levels). However, at higher MPs concentrations (5000 and 10,000 mg/L), AChE activity was significantly inhibited, stress indicators were significantly increased, and immune responses were significantly decreased. Our results indicate that acute exposure of P. fulvidraco to MPs had negligible effects at concentrations below 20 mg/L, whereas significant toxic effects such as AChE activity inhibition, stress responses, and immune suppression were observed at concentrations above 5000 mg/L. Therefore, our study highlights the risks of severe MPs pollution on aquatic ecosystems and fish health.

{"title":"Toxic effects of microplastics (polyethylene) exposure on acetylcholinesterase, stress indicators and immunity in Korean Bullhead, Pseudobagrus fulvidraco","authors":"Ju-Hyeong Lee , Jae-Ho Choi , Yue Jai Kang , Young Jae Choi , Cheol Young Choi , Ju-Chan Kang , Jun-Hwan Kim","doi":"10.1016/j.etap.2025.104654","DOIUrl":"10.1016/j.etap.2025.104654","url":null,"abstract":"<div><div>Microplastics (MPs) in aquatic environments can have toxic effects on various organisms, including fish. This study exposed <em>Pseudobagrus fulvidraco</em> to polyethylene MPs at 0, 10 mg/L (approximately 9.50 ×10<sup>8</sup> particles/L), 20 mg/L (approximately 1.9 ×10<sup>9</sup> particles/L), 5000 mg/L (approximately 4.75 ×10<sup>11</sup> particles/L), and 10,000 mg/L (approximately 9.50 ×10<sup>11</sup> particles/L) concentrations for 96 h. At relatively lower MPs concentrations (0, 10 and 20 mg/L), no significant changes were observed in acetylcholinesterase (AChE) activity, stress indicators (heat shock protein 70 and cortisol), or immune responses (lysozyme activity and immunoglobulin M levels). However, at higher MPs concentrations (5000 and 10,000 mg/L), AChE activity was significantly inhibited, stress indicators were significantly increased, and immune responses were significantly decreased. Our results indicate that acute exposure of <em>P. fulvidraco</em> to MPs had negligible effects at concentrations below 20 mg/L, whereas significant toxic effects such as AChE activity inhibition, stress responses, and immune suppression were observed at concentrations above 5000 mg/L. Therefore, our study highlights the risks of severe MPs pollution on aquatic ecosystems and fish health.</div></div>","PeriodicalId":11775,"journal":{"name":"Environmental toxicology and pharmacology","volume":"114 ","pages":"Article 104654"},"PeriodicalIF":4.2,"publicationDate":"2025-02-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143416584","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Multi-biomarker approach to assess the toxicity of carbamazepine, a neuropharmaceutical, in the female fish Astyanax lacustris (Teleostei: Characidae)

IF 4.2 3区环境科学与生态学 Q2 ENVIRONMENTAL SCIENCES

Environmental toxicology and pharmacology

Pub Date : 2025-02-11 DOI: 10.1016/j.etap.2025.104653

Amanda da Silveira Guerreiro , Guilherme de Aguiar , Cecilia Bertacini , Filipe Guilherme Andrade de Godoi , Giovana Souza Branco , Renato Massaaki Honji , Larissa Caminhas , Susanne Rath , Renata Guimarães Moreira

Carbamazepine (CBZ) is a pharmaceutical commonly used in the treatment of epilepsy and bipolar disorder and has been detected in different aquatic ecosystems worldwide. Considering its possible role in altering nervous system and reproduction, this study aimed to evaluate the effects of CBZ on molecular and cellular biomarkers of the teleost Astyanax lacustris. Results demonstrated that CBZ, in environmentally relevant concentrations (500 ng L⁻¹) increases fshβ gene expression levels, decreases muscle protein content and hepatic LPO (500 ng L⁻¹ and 1250 ng L⁻¹ of CBZ). Nonetheless, no effects were observed towards enzymatic activities, steroid plasma levels and/or lipid content. Considering that A. lacustris inhabits clean and polluted environments, it is possible to suggest that animals possess a level of tolerance to stressors, allowing them to maintain reproductive functions regardless of environmental challenges.

{"title":"Multi-biomarker approach to assess the toxicity of carbamazepine, a neuropharmaceutical, in the female fish Astyanax lacustris (Teleostei: Characidae)","authors":"Amanda da Silveira Guerreiro , Guilherme de Aguiar , Cecilia Bertacini , Filipe Guilherme Andrade de Godoi , Giovana Souza Branco , Renato Massaaki Honji , Larissa Caminhas , Susanne Rath , Renata Guimarães Moreira","doi":"10.1016/j.etap.2025.104653","DOIUrl":"10.1016/j.etap.2025.104653","url":null,"abstract":"<div><div>Carbamazepine (CBZ) is a pharmaceutical commonly used in the treatment of epilepsy and bipolar disorder and has been detected in different aquatic ecosystems worldwide. Considering its possible role in altering nervous system and reproduction, this study aimed to evaluate the effects of CBZ on molecular and cellular biomarkers of the teleost <em>Astyanax lacustris</em>. Results demonstrated that CBZ, in environmentally relevant concentrations (500 ng L<sup>−1</sup>) increases <em>fshβ</em> gene expression levels, decreases muscle protein content and hepatic LPO (500 ng L<sup>−1</sup> and 1250 ng L<sup>−1</sup> of CBZ). Nonetheless, no effects were observed towards enzymatic activities, steroid plasma levels and/or lipid content. Considering that <em>A. lacustris</em> inhabits clean and polluted environments, it is possible to suggest that animals possess a level of tolerance to stressors, allowing them to maintain reproductive functions regardless of environmental challenges.</div></div>","PeriodicalId":11775,"journal":{"name":"Environmental toxicology and pharmacology","volume":"114 ","pages":"Article 104653"},"PeriodicalIF":4.2,"publicationDate":"2025-02-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143416576","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Toxicokinetic and risk assessment of methylmercury in Indonesian pregnant population using physiologically-based toxicokinetic and reverse dosimetry modelling

IF 4.2 3区环境科学与生态学 Q2 ENVIRONMENTAL SCIENCES

Environmental toxicology and pharmacology

Pub Date : 2025-02-10 DOI: 10.1016/j.etap.2025.104655

Hasnawati Amqam , Rahayu Indriasari , Wahiduddin , Basir , Yusep Suparman

Methylmercury (MeHg) exposure poses significant risks, especially for pregnant women in Indonesia, where fish consumption is a common dietary habit. This study used a physiologically based toxicokinetic model and reverse dosimetry to estimate MeHg exposure levels and assess health risks in pregnant Indonesian women. The model was validated against biomarker data, with observed MeHg blood levels of 1.69 µg/L and 0.8 µg/L. The results from reverse dosimetry estimated a mean daily MeHg intake of 2.88 µg/day. Sensitivity analysis identified clearance rates and volume of distribution as key factors influencing MeHg levels in maternal and fetal compartments. The majority of the population had a hazard quotient below one, indicating safe exposure levels, however, a subset exceeded this threshold, indicating potential health risks. The study emphasizes the need for continuous monitoring and risk management strategies to reduce MeHg exposure in sensitive populations, particularly pregnant women.

{"title":"Toxicokinetic and risk assessment of methylmercury in Indonesian pregnant population using physiologically-based toxicokinetic and reverse dosimetry modelling","authors":"Hasnawati Amqam , Rahayu Indriasari , Wahiduddin , Basir , Yusep Suparman","doi":"10.1016/j.etap.2025.104655","DOIUrl":"10.1016/j.etap.2025.104655","url":null,"abstract":"<div><div>Methylmercury (MeHg) exposure poses significant risks, especially for pregnant women in Indonesia, where fish consumption is a common dietary habit. This study used a physiologically based toxicokinetic model and reverse dosimetry to estimate MeHg exposure levels and assess health risks in pregnant Indonesian women. The model was validated against biomarker data, with observed MeHg blood levels of 1.69 µg/L and 0.8 µg/L. The results from reverse dosimetry estimated a mean daily MeHg intake of 2.88 µg/day. Sensitivity analysis identified clearance rates and volume of distribution as key factors influencing MeHg levels in maternal and fetal compartments. The majority of the population had a hazard quotient below one, indicating safe exposure levels, however, a subset exceeded this threshold, indicating potential health risks. The study emphasizes the need for continuous monitoring and risk management strategies to reduce MeHg exposure in sensitive populations, particularly pregnant women.</div></div>","PeriodicalId":11775,"journal":{"name":"Environmental toxicology and pharmacology","volume":"114 ","pages":"Article 104655"},"PeriodicalIF":4.2,"publicationDate":"2025-02-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143394106","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Analysis of key proinflammatory mechanisms in cardiovascular pathology through stimulation with lipopolysaccharide and urban particulate matter in mouse atrial cardiomyocytes

IF 4.2 3区环境科学与生态学 Q2 ENVIRONMENTAL SCIENCES

Environmental toxicology and pharmacology

Pub Date : 2025-02-09 DOI: 10.1016/j.etap.2025.104652

Darío Mandaglio-Collados , Antonio José Ruiz-Alcaraz , José Miguel Rivera-Caravaca , María Pilar Ramos-Bratos , Francisco Marín , Raquel López-Gálvez

Air pollution has emerged as one of the leading causes of mortality, aggravating cardiovascular diseases. Urban-particulate matter (PM) can accumulate in the cardiovascular system and through inflammation, trigger systemic damage. One of the key mechanisms of this process could be related to the activation of the inflammasome through the pre-existence of a low-grade endotoxemia and PM presence in the cells. Herein, we studied the deleterious effects of urban-PM and Lipopolysaccharide (LPS) exposure in a HL-1 mouse cardiomyocyte cell line. Urban-PM induced biological changes, including mRNA expression of pro-inflammatory genes, intracellular reactive oxygen species (ROS) generation and overexpression of inflammasome-related and structural proteins. The results revealed that urban-PM with different ultrastructure, as determined by transmission electron microscopy (TEM), is embedded inside the cardiomyocytes, leading to the recognition and activation of the inflammatory process. The increase of ROS levels and mRNA levels of pro-inflammatory genes were similarly observed in a dose-dependent manner. In addition, components and proteins of the inflammasome such as associated speck-like protein containing a CARD (ASC), caspase-1 and IL-1β were differentially overexpressed in treated HL-1 cells, as well as structural proteins like Connexin 43 (Cx43). These results provide new insights into the mechanisms that mediate innate pro-inflammatory activation in cardiomyocytes in response to air suspension pollutants.

{"title":"Analysis of key proinflammatory mechanisms in cardiovascular pathology through stimulation with lipopolysaccharide and urban particulate matter in mouse atrial cardiomyocytes","authors":"Darío Mandaglio-Collados , Antonio José Ruiz-Alcaraz , José Miguel Rivera-Caravaca , María Pilar Ramos-Bratos , Francisco Marín , Raquel López-Gálvez","doi":"10.1016/j.etap.2025.104652","DOIUrl":"10.1016/j.etap.2025.104652","url":null,"abstract":"<div><div>Air pollution has emerged as one of the leading causes of mortality, aggravating cardiovascular diseases. Urban-particulate matter (PM) can accumulate in the cardiovascular system and through inflammation, trigger systemic damage. One of the key mechanisms of this process could be related to the activation of the inflammasome through the pre-existence of a low-grade endotoxemia and PM presence in the cells. Herein, we studied the deleterious effects of urban-PM and Lipopolysaccharide (LPS) exposure in a HL-1 mouse cardiomyocyte cell line. Urban-PM induced biological changes, including mRNA expression of pro-inflammatory genes, intracellular reactive oxygen species (ROS) generation and overexpression of inflammasome-related and structural proteins. The results revealed that urban-PM with different ultrastructure, as determined by transmission electron microscopy (TEM), is embedded inside the cardiomyocytes, leading to the recognition and activation of the inflammatory process. The increase of ROS levels and mRNA levels of pro-inflammatory genes were similarly observed in a dose-dependent manner. In addition, components and proteins of the inflammasome such as associated speck-like protein containing a CARD (ASC), caspase-1 and IL-1β were differentially overexpressed in treated HL-1 cells, as well as structural proteins like Connexin 43 (Cx43). These results provide new insights into the mechanisms that mediate innate pro-inflammatory activation in cardiomyocytes in response to air suspension pollutants.</div></div>","PeriodicalId":11775,"journal":{"name":"Environmental toxicology and pharmacology","volume":"114 ","pages":"Article 104652"},"PeriodicalIF":4.2,"publicationDate":"2025-02-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143388259","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluation of sevoflurane volatilization from gel and microsphere formulations and prediction of workplace exposure

IF 4.2 3区环境科学与生态学 Q2 ENVIRONMENTAL SCIENCES

Environmental toxicology and pharmacology

Pub Date : 2025-02-04 DOI: 10.1016/j.etap.2025.104651

María Trinidad Gómez-Sánchez , F.Dámaso Fernández-Ginés , Sergio Parra , Manuel Gerónimo-Pardo

The general inhalation anesthetic sevoflurane can be used as a topical analgesic in patients with painful wounds. For this new indication, it is irrigated in its liquid formulation into the wound bed, which inevitably causes room pollution. This investigation aimed to analyze the environmental contamination caused by new sevoflurane gel and microsphere formulations at different concentrations (50 %, 30 %, and 10 %) to find which would produce air levels below 2 ppm. The weight loss over time of each formulation was recorded to calculate the amount of sevoflurane volatilized, and the associated room pollution was further calculated under demanding conditions (a small 12 m³ room with three air exchanges per hour, a patient treated every 30 min). Air levels below 2 ppm were found for the gel at 10 % and the microspheres at 30 %. Both new formulations would reduce the environmental pollution caused by unformulated sevoflurane, further improving occupational safety when treating painful wounds.

{"title":"Evaluation of sevoflurane volatilization from gel and microsphere formulations and prediction of workplace exposure","authors":"María Trinidad Gómez-Sánchez , F.Dámaso Fernández-Ginés , Sergio Parra , Manuel Gerónimo-Pardo","doi":"10.1016/j.etap.2025.104651","DOIUrl":"10.1016/j.etap.2025.104651","url":null,"abstract":"<div><div>The general inhalation anesthetic sevoflurane can be used as a topical analgesic in patients with painful wounds. For this new indication, it is irrigated in its liquid formulation into the wound bed, which inevitably causes room pollution. This investigation aimed to analyze the environmental contamination caused by new sevoflurane gel and microsphere formulations at different concentrations (50 %, 30 %, and 10 %) to find which would produce air levels below 2 ppm. The weight loss over time of each formulation was recorded to calculate the amount of sevoflurane volatilized, and the associated room pollution was further calculated under demanding conditions (a small 12 m<sup>3</sup> room with three air exchanges per hour, a patient treated every 30 min). Air levels below 2 ppm were found for the gel at 10 % and the microspheres at 30 %. Both new formulations would reduce the environmental pollution caused by unformulated sevoflurane, further improving occupational safety when treating painful wounds.</div></div>","PeriodicalId":11775,"journal":{"name":"Environmental toxicology and pharmacology","volume":"114 ","pages":"Article 104651"},"PeriodicalIF":4.2,"publicationDate":"2025-02-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143307893","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Investigating the effects of anthropogenic yttrium contamination: Biochemical alterations in the gills and digestive gland of exposed mussels (Mytilus galloprovincialis)

IF 4.2 3区环境科学与生态学 Q2 ENVIRONMENTAL SCIENCES

Environmental toxicology and pharmacology

Pub Date : 2025-02-03 DOI: 10.1016/j.etap.2025.104650

João Pinto , Marta Cunha , Carla Leite , Amadeu M.V.M. Soares , Rosa Freitas , Eduarda Pereira

The rapid growth of electronic waste, including fluorescent lamp waste (FLW), has led to an increase in elements like yttrium (Y) and other rare earth elements (REE) in aquatic environments due to improper waste management. However, there are no guidelines for permissible concentrations of these elements in the environment. This study examines the biochemical impacts of varying Y concentrations (50, 100, and 200 µg L⁻¹), mimicking a FLW discharge, on the gills and digestive gland of Mytilus galloprovincialis exposed for 14 days. Energy reserves, antioxidant and biotransformation enzymes, lipid peroxidation (LPO), and neurotoxicity were measured. Results showed a limited antioxidant capacity in the gills at the highest Y concentration. Additionally, increased LPO levels in both organs suggest oxidative stress-induced damage. The study underscores the need for regulations to address Y contamination and safeguard aquatic ecosystems, proposing a reference value for permissible concentrations based on the observed effects.

{"title":"Investigating the effects of anthropogenic yttrium contamination: Biochemical alterations in the gills and digestive gland of exposed mussels (Mytilus galloprovincialis)","authors":"João Pinto , Marta Cunha , Carla Leite , Amadeu M.V.M. Soares , Rosa Freitas , Eduarda Pereira","doi":"10.1016/j.etap.2025.104650","DOIUrl":"10.1016/j.etap.2025.104650","url":null,"abstract":"<div><div>The rapid growth of electronic waste, including fluorescent lamp waste (FLW), has led to an increase in elements like yttrium (Y) and other rare earth elements (REE) in aquatic environments due to improper waste management. However, there are no guidelines for permissible concentrations of these elements in the environment. This study examines the biochemical impacts of varying Y concentrations (50, 100, and 200 µg L<sup>−1</sup>), mimicking a FLW discharge, on the gills and digestive gland of <em>Mytilus galloprovincialis</em> exposed for 14 days. Energy reserves, antioxidant and biotransformation enzymes, lipid peroxidation (LPO), and neurotoxicity were measured. Results showed a limited antioxidant capacity in the gills at the highest Y concentration. Additionally, increased LPO levels in both organs suggest oxidative stress-induced damage. The study underscores the need for regulations to address Y contamination and safeguard aquatic ecosystems, proposing a reference value for permissible concentrations based on the observed effects.</div></div>","PeriodicalId":11775,"journal":{"name":"Environmental toxicology and pharmacology","volume":"114 ","pages":"Article 104650"},"PeriodicalIF":4.2,"publicationDate":"2025-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143257444","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Diverse alterations and correlations in antioxidant gene expression in honeybee (Apis mellifera) hemocytes interacting with microbial pathogen-associated molecular patterns and pesticide cocktails

IF 4.2 3区环境科学与生态学 Q2 ENVIRONMENTAL SCIENCES

Environmental toxicology and pharmacology

Pub Date : 2025-02-01 DOI: 10.1016/j.etap.2025.104649

Dani Sukkar , Lea Wagner , Antoine Bonnefoy , Jairo Falla-Angel , Philippe Laval-Gilly

Imidacloprid and amitraz, two common pesticides, affect honeybee health, behavior, and pathogen resistance. Understanding multi-risk exposures is vital to explaining their role in hive performance decline and colony health. This study assessed antioxidant system genes to evaluate pesticide impacts on immune response and enzymes responsible for hydrogen peroxide (H₂O₂) production. Honeybee hemocytes were exposed to imidacloprid and amitraz at various concentrations, alongside pathogen-associated molecular patterns (PAMPs) like zymosan A (ZYM), lipopolysaccharide (LPS), and peptidoglycan (PGN). Imidacloprid primarily affects mitochondrial components like mitochondrial superoxide dismutase (MnSOD), while amitraz decreases the expression of both mitochondrial and cytosolic genes (MnSOD, DUOX, and CuZnSOD). PAMPs modulate antioxidant responses, with LPS showing the strongest impact. DUOX and the phenoloxidase system are consistently linked. While AmPPO (prophenoloxidase) involved in melanization is less affected, pesticides significantly disrupt H₂O₂ production and antioxidant defenses, complicating risk assessment robustness.

{"title":"Diverse alterations and correlations in antioxidant gene expression in honeybee (Apis mellifera) hemocytes interacting with microbial pathogen-associated molecular patterns and pesticide cocktails","authors":"Dani Sukkar , Lea Wagner , Antoine Bonnefoy , Jairo Falla-Angel , Philippe Laval-Gilly","doi":"10.1016/j.etap.2025.104649","DOIUrl":"10.1016/j.etap.2025.104649","url":null,"abstract":"<div><div>Imidacloprid and amitraz, two common pesticides, affect honeybee health, behavior, and pathogen resistance. Understanding multi-risk exposures is vital to explaining their role in hive performance decline and colony health. This study assessed antioxidant system genes to evaluate pesticide impacts on immune response and enzymes responsible for hydrogen peroxide (H₂O₂) production. Honeybee hemocytes were exposed to imidacloprid and amitraz at various concentrations, alongside pathogen-associated molecular patterns (PAMPs) like zymosan A (ZYM), lipopolysaccharide (LPS), and peptidoglycan (PGN). Imidacloprid primarily affects mitochondrial components like mitochondrial superoxide dismutase (MnSOD), while amitraz decreases the expression of both mitochondrial and cytosolic genes (MnSOD, DUOX, and CuZnSOD). PAMPs modulate antioxidant responses, with LPS showing the strongest impact. DUOX and the phenoloxidase system are consistently linked. While AmPPO (prophenoloxidase) involved in melanization is less affected, pesticides significantly disrupt H₂O₂ production and antioxidant defenses, complicating risk assessment robustness.</div></div>","PeriodicalId":11775,"journal":{"name":"Environmental toxicology and pharmacology","volume":"114 ","pages":"Article 104649"},"PeriodicalIF":4.2,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143077723","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mitigation of experimental hepatocellular Carcinoma by Inonotus obliquus Mushroom extract in association with oxidative stress and inflammation signaling

IF 4.2 3区环境科学与生态学 Q2 ENVIRONMENTAL SCIENCES

Environmental toxicology and pharmacology

Pub Date : 2025-01-30 DOI: 10.1016/j.etap.2025.104648

Zaved Ahmad , Pir Mohammad Ishfaq , Subodh Kumar Jain , Siddhartha Kumar Mishra

Chaga mushroom (Inonotus obliquus) has been a component of folk medicine treating several disorders mainly through its anti-inflammatory and antioxidant properties, yet its effects on liver carcinoma needed elucidation. This study investigated the effect of an aqueous extract of Inonotus obliquus (IOAE) against diethyl-nitrosamine/carbon tetrachloride (DEN/CCl₄) induced HCC in mice and addressed its molecular mechanism. HCC was induced by a single intraperitoneal injection (i.p.) of DEN (1 mg/kg b.w.) followed by CCl₄ (0.2 ml/kg, i.p., twice a week) after six weeks. IOAE (200 mg/kg b.w.) was administered orally after the induction of HCC. Physiological and hematological parameters and biochemical assays for oxidative stress markers were performed. Histopathological and immunohistochemistry for inflammation and apoptosis were performed. DEN/CCl₄ caused a reduction in mice body weight and an increase in the liver weight which was significantly restored by IOAE administration. The tumor incidence of DEN/CCl₄ (100 %) was reduced to about 25 % by IOAE supplementation. DEN/CCl₄ caused alterations in the hematological parameters, serum total protein albumin globulin, A/G ratio, liver function markers (aspartate aminotransferase, alanine aminotransferase, alkaline phosphatase gamma-glutamyl transferase, acid phosphatase and bilirubin) and lipid profile markers that were significantly restored by IOAE administration. Oxidative stress markers (malondialdehyde, superoxide dismutase, catalase, nitric oxide, lactate dehydrogenase, and glutathione-s-transferase) were reduced by DEN/CCl₄ which were significantly restored by IOAE treatment. The liver histopathology alterations caused by DEN/CCl₄ were significantly ameliorated by IOAE treatment. Immunohistochemical studies suggest that AFP, caspase-3, COX-2, and iNOS were chronically overexpressed in DEN/CCl₄-exposed mice which were notably attenuated by IOAE administration. Collectively IOAE was found to suppress tumor incidence by suppressing iNOS-COX-2-dependent inflammation and caspase-3 mediated apoptosis. Chaga mushroom showed remarkable anticancer effects against liver carcinoma through induction of apoptosis and suppression of inflammation.

{"title":"Mitigation of experimental hepatocellular Carcinoma by Inonotus obliquus Mushroom extract in association with oxidative stress and inflammation signaling","authors":"Zaved Ahmad , Pir Mohammad Ishfaq , Subodh Kumar Jain , Siddhartha Kumar Mishra","doi":"10.1016/j.etap.2025.104648","DOIUrl":"10.1016/j.etap.2025.104648","url":null,"abstract":"<div><div>Chaga mushroom (<em>Inonotus obliquus</em>) has been a component of folk medicine treating several disorders mainly through its anti-inflammatory and antioxidant properties, yet its effects on liver carcinoma needed elucidation. This study investigated the effect of an aqueous extract of <em>Inonotus obliquus</em> (IOAE) against diethyl-nitrosamine/carbon tetrachloride (DEN/CCl<sub>4</sub>) induced HCC in mice and addressed its molecular mechanism. HCC was induced by a single intraperitoneal injection (i.p.) of DEN (1 mg/kg b.w.) followed by CCl<sub>4</sub> (0.2 ml/kg, i.p., twice a week) after six weeks. IOAE (200 mg/kg b.w.) was administered orally after the induction of HCC. Physiological and hematological parameters and biochemical assays for oxidative stress markers were performed. Histopathological and immunohistochemistry for inflammation and apoptosis were performed. DEN/CCl<sub>4</sub> caused a reduction in mice body weight and an increase in the liver weight which was significantly restored by IOAE administration. The tumor incidence of DEN/CCl<sub>4</sub> (100 %) was reduced to about 25 % by IOAE supplementation. DEN/CCl<sub>4</sub> caused alterations in the hematological parameters, serum total protein albumin globulin, A/G ratio, liver function markers (aspartate aminotransferase, alanine aminotransferase, alkaline phosphatase gamma-glutamyl transferase, acid phosphatase and bilirubin) and lipid profile markers that were significantly restored by IOAE administration. Oxidative stress markers (malondialdehyde, superoxide dismutase, catalase, nitric oxide, lactate dehydrogenase, and glutathione-s-transferase) were reduced by DEN/CCl<sub>4</sub> which were significantly restored by IOAE treatment. The liver histopathology alterations caused by DEN/CCl<sub>4</sub> were significantly ameliorated by IOAE treatment. Immunohistochemical studies suggest that AFP, caspase-3, COX-2, and iNOS were chronically overexpressed in DEN/CCl<sub>4</sub>-exposed mice which were notably attenuated by IOAE administration. Collectively IOAE was found to suppress tumor incidence by suppressing iNOS-COX-2-dependent inflammation and caspase-3 mediated apoptosis. Chaga mushroom showed remarkable anticancer effects against liver carcinoma through induction of apoptosis and suppression of inflammation.</div></div>","PeriodicalId":11775,"journal":{"name":"Environmental toxicology and pharmacology","volume":"114 ","pages":"Article 104648"},"PeriodicalIF":4.2,"publicationDate":"2025-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143076740","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0