Chrysanthemum lavandulifolium homolog CYCLIN A2;1 modulates cell division in ray florets.

IF 5.6 2区 生物学 Q1 PLANT SCIENCES Journal of Experimental Botany Pub Date : 2024-10-30 DOI:10.1093/jxb/erae325
Peng Zhang, Yahui Wang, Zhimin Wang, Shengqiang Di, Xinyi Zhang, Di Ma, Zhilong Bao, Fangfang Ma
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Abstract

The morphology of ray florets in chrysanthemums is tightly associated with cell division and expansion, both of which require proper progression of the cell cycle. Here, we identified a Chrysanthemum lavandulifolium homolog, CYCLIN A2;1 (CYCA2;1), the expression of which in ray florets is negatively correlated with petal width. We found that CYC2a, a TEOSINTE BRANCHED1/CYCLOIDEA/PROLIFERATING CELL FACTOR (TCP) transcription factor in the CYCLOIDEA2 (CYC2) family, interacts with and stabilizes CYC2b, and the latter can bind to the promoter of CYCA2;1 to activate its transcription. Overexpression of CYCA2;1 in C. lavandulifolium reduced the size of capitula and ray florets. Cytological analysis revealed that CYCA2;1 overexpression inhibited both cell division and expansion via repression of the mitotic cell cycle in ray florets, the latitudinal development of which was more relatively negatively influenced, thereby leading to increased ratios of petal length to width at later developmental stages. Yeast two-hybrid library screening revealed multiple proteins that interacted with CYCA2;1 including ACTIN-RELATED PROTEIN7 (ARP7), and silencing ARP7 inhibited the development of ray florets. Co-immunoprecipitation assays confirmed that CYCA2;1 could induce the degradation of ARP7 to inhibit the development of ray florets. Taken together, our results indicate the presence of a regulatory network in ray floret development in chrysanthemum consisting of CYC2b-CYCA2;1-ARP7 that acts via governing mitosis. The identification of this network has the potential to facilitate breeding efforts targeted at producing novel ornamental traits in the flowers.

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Chrysanthemum lavandulifolium homolog ClCYCA2;1 modulates cell division in ray florets.
菊花射线小花的形态与细胞分裂和细胞扩张密切相关,而细胞分裂和细胞扩张都需要细胞周期的正常进行。在这里,我们发现了一种菊花同源物 ClCYCA2;1,它在光线小花中的表达与花瓣宽度呈负相关。CYCLOIDEA2(CYC2)家族中的两个 TCP 转录因子 ClCYC2a 与 ClCYC2b 相互作用并稳定 ClCYC2b,后者能与 ClCYCA2;1 的启动子结合,激活其转录。在 C. lavandulifolium 中过表达 ClCYCA2;1 会减小头状花序和射线小花的大小。细胞学分析表明,ClCYCA2;1 的过表达会通过抑制射线小花的有丝分裂细胞周期来抑制细胞分裂和细胞扩增,射线小花的纬向发育会受到更大的负面影响,导致后期发育阶段花瓣长宽比增加。酵母二杂交文库筛选揭示了包括 ARP7 在内的多个 ClCYCA2;1 相互作用蛋白,沉默 ClARP7 可抑制射线小花的发育。共免疫沉淀试验证实,ClCYCA2;1能诱导ClARP7降解,从而抑制射线小花的发育。综上所述,我们的研究构成了一个包含 ClCYC2b-ClCYCA2;1-ClARP7 的调控网络,该网络通过调控有丝分裂参与射线小花的发育,这可能有助于菊花新观赏性状的育种工作。
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来源期刊
Journal of Experimental Botany
Journal of Experimental Botany 生物-植物科学
CiteScore
12.30
自引率
4.30%
发文量
450
审稿时长
1.9 months
期刊介绍: The Journal of Experimental Botany publishes high-quality primary research and review papers in the plant sciences. These papers cover a range of disciplines from molecular and cellular physiology and biochemistry through whole plant physiology to community physiology. Full-length primary papers should contribute to our understanding of how plants develop and function, and should provide new insights into biological processes. The journal will not publish purely descriptive papers or papers that report a well-known process in a species in which the process has not been identified previously. Articles should be concise and generally limited to 10 printed pages.
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