{"title":"Programmable DNA hydrogels for biosensing and point-of-care test","authors":"","doi":"10.1016/j.ccr.2024.216084","DOIUrl":null,"url":null,"abstract":"<div><p>The double-helix polymer deoxyribonucleic acid (DNA) demonstrates specificity and programmability via complementary base pairing, making it a biometric component for target recognition. Meanwhile, hydrogel as a three-dimensional polymer can effectively isolate interfering substances in complex sample matrices, with the distinctive stimulus–response and gel-sol properties bolster their efficacy in biosensing applications. Therefore, the combination of DNA and hydrogel attributes renders DNA hydrogels a superior option in biosensing and point-of-care test (POCT). To prove the biosensing benefits of DNA hydrogels, this review provides a comprehensive overview of recent advancements in DNA hydrogels, focusing on their preparation, biosensing, and application. The common preparation methods of DNA hydrogels were introduced first, followed by a comprehensive summary of signal amplification strategies in biosensing and the application of DNA hydrogel-based biosensors. Furthermore, the limitations of DNA hydrogels in biosensing and POCT as well as future developments were also discussed. This review aims to stimulate interest regarding DNA hydrogels, with the hope that it can address the challenges encountered in the practical use of biosensing and POCT.</p></div>","PeriodicalId":289,"journal":{"name":"Coordination Chemistry Reviews","volume":null,"pages":null},"PeriodicalIF":20.3000,"publicationDate":"2024-07-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Coordination Chemistry Reviews","FirstCategoryId":"92","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0010854524004302","RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"CHEMISTRY, INORGANIC & NUCLEAR","Score":null,"Total":0}
引用次数: 0
Abstract
The double-helix polymer deoxyribonucleic acid (DNA) demonstrates specificity and programmability via complementary base pairing, making it a biometric component for target recognition. Meanwhile, hydrogel as a three-dimensional polymer can effectively isolate interfering substances in complex sample matrices, with the distinctive stimulus–response and gel-sol properties bolster their efficacy in biosensing applications. Therefore, the combination of DNA and hydrogel attributes renders DNA hydrogels a superior option in biosensing and point-of-care test (POCT). To prove the biosensing benefits of DNA hydrogels, this review provides a comprehensive overview of recent advancements in DNA hydrogels, focusing on their preparation, biosensing, and application. The common preparation methods of DNA hydrogels were introduced first, followed by a comprehensive summary of signal amplification strategies in biosensing and the application of DNA hydrogel-based biosensors. Furthermore, the limitations of DNA hydrogels in biosensing and POCT as well as future developments were also discussed. This review aims to stimulate interest regarding DNA hydrogels, with the hope that it can address the challenges encountered in the practical use of biosensing and POCT.
双螺旋聚合物脱氧核糖核酸(DNA)通过互补碱基配对显示出特异性和可编程性,使其成为目标识别的生物识别元件。同时,水凝胶作为一种三维聚合物,能有效隔离复杂样品基质中的干扰物质,其独特的刺激-响应和凝胶-溶液特性增强了其在生物传感应用中的功效。因此,DNA 和水凝胶属性的结合使 DNA 水凝胶成为生物传感和床旁检测(POCT)的上佳选择。为了证明 DNA 水凝胶的生物传感优势,本综述全面概述了 DNA 水凝胶的最新进展,重点关注其制备、生物传感和应用。首先介绍了 DNA 水凝胶的常见制备方法,然后全面总结了生物传感中的信号放大策略以及基于 DNA 水凝胶的生物传感器的应用。此外,还讨论了 DNA 水凝胶在生物传感和 POCT 中的局限性以及未来的发展。本综述旨在激发人们对 DNA 水凝胶的兴趣,希望它能解决生物传感和 POCT 实际应用中遇到的挑战。
期刊介绍:
Coordination Chemistry Reviews offers rapid publication of review articles on current and significant topics in coordination chemistry, encompassing organometallic, supramolecular, theoretical, and bioinorganic chemistry. It also covers catalysis, materials chemistry, and metal-organic frameworks from a coordination chemistry perspective. Reviews summarize recent developments or discuss specific techniques, welcoming contributions from both established and emerging researchers.
The journal releases special issues on timely subjects, including those featuring contributions from specific regions or conferences. Occasional full-length book articles are also featured. Additionally, special volumes cover annual reviews of main group chemistry, transition metal group chemistry, and organometallic chemistry. These comprehensive reviews are vital resources for those engaged in coordination chemistry, further establishing Coordination Chemistry Reviews as a hub for insightful surveys in inorganic and physical inorganic chemistry.