Immunomodulatory Potential of Human Testicular Tissue-Derived Mesenchymal Stem/Stromal Cells over the Lifespan

Q4 Biochemistry, Genetics and Molecular Biology Cell and Tissue Biology Pub Date : 2024-08-06 DOI:10.1134/s1990519x24700329

Maryam Hassan Nasab, Mahmood Dehghani-Ashkezari, Fateme Montazeri, Ehsan Farashahi-Yazd, Fatemeh Hajizadeh-Tafti, Jalal Golzadeh, Seyed Mehdi Hoseini, Behrouz Aflatoonian

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Abstract

The sequential culture expansion has been shown to affect the favorable characteristics of human mesenchymal stem/stromal cells (hMSCs), including their immunomodulatory potential. Hereby, we have investigated the immunomodulatory potential of Yazd human testicular Cells (YhTCs) regarding gene expression profile assessment during their lifespan. Morphological examination, immunostaining of vimentin and fibronectin markers, and flow cytometry analysis were used to confirm cultured cells’ MSC properties. Quantitative RT-PCR was used to investigate the target genes, COX-1, TGF-β, IL-6, VCAM-1, and FAS, at different passage numbers (P3, P6, and P9). Our findings confirmed the MSC properties of YhTCs over the lifespan. The results of COX-1 and IL-6 indicated significantly higher expression levels at the late passages (p‑value = 0.03 and p-value = 0.01, respectively). Despite the similar expression pattern, VCAM-1 expression altered significantly at different passages (p-value = 0.0006), but the changes in TGF-β expression levels were non-significant across the lifespan. The YhTCs maintained the main characteristics of MSCs over the sequential culture expansion. Although some of the target genes in the study were sensitive to the culture expansion in the unstimulated YhTCs (IL-6, COX-1, and VCAM1), it seems that FAS and TGF-β need external stimuli or intracellular signals to be induced in YhTCs.

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人睾丸组织来源间充质干细胞/基质细胞在整个生命周期的免疫调节潜力

摘要已有研究表明，连续培养扩增会影响人类间充质干/基质细胞（hMSCs）的有利特性，包括其免疫调节潜力。在此，我们研究了亚兹德人睾丸细胞（YhTCs）在其生命周期中基因表达谱评估方面的免疫调节潜力。形态学检查、波形蛋白和纤维连接蛋白标记免疫染色以及流式细胞术分析被用来确认培养细胞的间充质干细胞特性。定量 RT-PCR 被用来研究不同生长期（P3、P6 和 P9）的 COX-1、TGF-β、IL-6、VCAM-1 和 FAS 等靶基因。我们的研究结果证实了 YhTCs 在整个生命周期中的间充质干细胞特性。COX-1和IL-6在晚期的表达水平明显更高（p值分别为0.03和0.01）。尽管表达模式相似，但VCAM-1的表达在不同传代有显著变化（p值=0.0006），但TGF-β的表达水平在整个生命周期的变化不显著。YhTCs在连续培养扩增过程中保持了间充质干细胞的主要特征。尽管研究中的一些靶基因（IL-6、COX-1和VCAM1）对未受刺激的YhTCs的培养扩增敏感，但看来FAS和TGF-β需要外部刺激或细胞内信号才能在YhTCs中被诱导。

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来源期刊

Cell and Tissue Biology Biochemistry, Genetics and Molecular Biology-Cell Biology

CiteScore

0.80

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0.00%

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期刊介绍： The journal publishes papers on vast aspects of cell research, including morphology, biochemistry, biophysics, genetics, molecular biology, immunology. The journal accepts original experimental studies, theoretical articles suggesting novel principles and approaches, presentations of new hypotheses, reviews highlighting major developments in cell biology, discussions. The main objective of the journal is to provide a competent representation and integration of research made on cells (animal and plant cells, both in vivo and in cell culture) offering insight into the structure and functions of live cells as a whole. Characteristically, the journal publishes articles on biology of free-living and parasitic protists, which, unlike Metazoa, are eukaryotic organisms at the cellular level of organization.