Novel GaN-Based Substrates with Gold Nanostructures for Ultra-Sensitive SERS Analysis: Micro-Nano Pit Morphology for Enhanced Molecular Detection

IF 1.6 4区 医学 Q4 ENGINEERING, BIOMEDICAL Journal of Medical and Biological Engineering Pub Date : 2024-08-05 DOI:10.1007/s40846-024-00889-1
Tsung-Shine Ko, Chen-An Deng, Jiann Shieh, Hung Ji Huang, Yung-Sheng Lin, Yang-Wei Lin, Yi-Chun Du
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Abstract

Purpose

Surface-enhanced Raman scattering (SERS) is a technique for trace analysis detection based on the interaction of light with matter and between materials. In the past development of SERS, precious metals were primarily chosen as substrates due to their high electromagnetic effect, which leads to significantly enhanced SERS signals. However, the effect of using only precious metals is limited. Therefore, this study utilizes the characteristic micro-nano V-shaped pits that appear on the surface of c-plane GaN after wet etching. By depositing a gold film of various thicknesses, we aim to increase the contact area with the target molecule Rhodamine 6G (R6G), thereby further enhancing the sensitivity of SERS detection.

Methods

After fabricating pitted c-plane GaN using chemical etching techniques, we analyzed the sample surface with a scanning electron microscope and assessed the impact of different gold film thicknesses on the SERS intensity of R6G using Raman spectroscopy. The comprehensive biomedical detection effectiveness was also evaluated using contact angle measurement, and fluorescence microscopy.

Results

For the target molecule R6G, after depositing a 25 nm gold film, the enhancement factor of the substrate for detection reached 2.21×108, and the limit of detection was achieved at a concentration of 10− 10 M.

Conclusion

This study confirms the feasibility of using wet etching techniques on hexagonal materials like GaN for SERS applications. The GaN substrate with V-shaped pits provides an increased surface area, effectively enhancing SERS signal strength. This offers different choices and perspectives for SERS substrate selection in the detection of various target molecules.

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用于超灵敏 SERS 分析的带有金纳米结构的新型氮化镓基底板:用于增强分子检测的微纳坑形态学
目的表面增强拉曼散射(SERS)是一种基于光与物质以及材料之间相互作用的痕量分析检测技术。在过去的 SERS 发展中,主要选择贵金属作为基底,因为贵金属具有高电磁效应,可显著增强 SERS 信号。然而,仅使用贵金属的效果有限。因此,本研究利用了湿法蚀刻后在 c 平面氮化镓表面出现的特征性微纳 V 形坑。通过沉积不同厚度的金膜,我们旨在增加与目标分子罗丹明 6G(R6G)的接触面积,从而进一步提高 SERS 检测的灵敏度。方法利用化学蚀刻技术制作出有凹坑的 c-plane GaN 后,我们用扫描电子显微镜分析了样品表面,并用拉曼光谱评估了不同厚度的金膜对 R6G SERS 强度的影响。结果对于目标分子 R6G,在沉积 25 nm 金膜后,基底的检测增强因子达到 2.21×108,在浓度为 10- 10 M 时达到检测极限。带有 V 形凹坑的 GaN 衬底增大了表面积,有效增强了 SERS 信号强度。这为检测各种目标分子时选择 SERS 基底提供了不同的选择和前景。
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来源期刊
CiteScore
4.30
自引率
5.00%
发文量
81
审稿时长
3 months
期刊介绍: The purpose of Journal of Medical and Biological Engineering, JMBE, is committed to encouraging and providing the standard of biomedical engineering. The journal is devoted to publishing papers related to clinical engineering, biomedical signals, medical imaging, bio-informatics, tissue engineering, and so on. Other than the above articles, any contributions regarding hot issues and technological developments that help reach the purpose are also included.
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