E. Lorenti, D. Faccone, J. Origlia, F. Maydup, H. Nievas, A. Corso, G. Daneri, A. Harrington, S. Lucero, E. Varela, G. Giacoboni
{"title":"Antimicrobial resistance occurrence in the South American Sea Lion (Otaria flavescens) from Patagonia, Argentina, from a One Health perspective","authors":"E. Lorenti, D. Faccone, J. Origlia, F. Maydup, H. Nievas, A. Corso, G. Daneri, A. Harrington, S. Lucero, E. Varela, G. Giacoboni","doi":"10.1007/s00300-024-03291-4","DOIUrl":null,"url":null,"abstract":"<p>Under the \"One Health\" concept, this paper aims to explore the occurrence of antimicrobial resistance in Enterobacterales (i.e., <i>Escherichia coli</i> and <i>Salmonella enterica</i>) among the South American Sea Lions on Argentina's northern Patagonian coast. From 177 fecal samples collected, conventional protocols were used to isolate <i>E. coli</i> and <i>S. enterica</i>. Antimicrobials classified as critically important by the WHO were evaluated by disk diffusion and agar spot (colistin) methods. Molecular tools were used to detect <i>mcr</i> genes as needed. A total of 176 antimicrobial-susceptible <i>E. coli</i> strains and 14 <i>S. enterica</i> strains were recovered. Twelve <i>E. coli</i> strains were colistin resistant (agar spot positive) but PCR negative for the <i>mcr-1</i> to <i>mcr-5</i> genes. Whole-genome sequencing revealed a chromosomal mutation, coding for Val161Gly change in PmrB protein, as the colistin resistance mechanism in one of these isolates. This study provides knowledge on AMR surveillance in wildlife associated with the marine environment in South America.</p>","PeriodicalId":20362,"journal":{"name":"Polar Biology","volume":"90 1","pages":""},"PeriodicalIF":1.5000,"publicationDate":"2024-08-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Polar Biology","FirstCategoryId":"93","ListUrlMain":"https://doi.org/10.1007/s00300-024-03291-4","RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"BIODIVERSITY CONSERVATION","Score":null,"Total":0}
引用次数: 0
Abstract
Under the "One Health" concept, this paper aims to explore the occurrence of antimicrobial resistance in Enterobacterales (i.e., Escherichia coli and Salmonella enterica) among the South American Sea Lions on Argentina's northern Patagonian coast. From 177 fecal samples collected, conventional protocols were used to isolate E. coli and S. enterica. Antimicrobials classified as critically important by the WHO were evaluated by disk diffusion and agar spot (colistin) methods. Molecular tools were used to detect mcr genes as needed. A total of 176 antimicrobial-susceptible E. coli strains and 14 S. enterica strains were recovered. Twelve E. coli strains were colistin resistant (agar spot positive) but PCR negative for the mcr-1 to mcr-5 genes. Whole-genome sequencing revealed a chromosomal mutation, coding for Val161Gly change in PmrB protein, as the colistin resistance mechanism in one of these isolates. This study provides knowledge on AMR surveillance in wildlife associated with the marine environment in South America.
期刊介绍:
Polar Biology publishes Original Papers, Reviews, and Short Notes and is the focal point for biologists working in polar regions. It is also of interest to scientists working in biology in general, ecology and physiology, as well as in oceanography and climatology related to polar life. Polar Biology presents results of studies in plants, animals, and micro-organisms of marine, limnic and terrestrial habitats in polar and subpolar regions of both hemispheres.
Taxonomy/ Biogeography
Life History
Spatio-temporal Patterns in Abundance and Diversity
Ecological Interactions
Trophic Ecology
Ecophysiology/ Biochemistry of Adaptation
Biogeochemical Pathways and Cycles
Ecological Models
Human Impact/ Climate Change/ Conservation