Healing mechanism of cotton bandage loaded with PNIPAM/GO-Ag hydrogel on the deep second-degree burn wound in rats model

IF 3.2 3区医学 Q2 CRITICAL CARE MEDICINE Burns Pub Date : 2024-08-04 DOI:10.1016/j.burns.2024.08.001

Ning Liu, Baoxia Xue, Wei Cheng, Yong Liu, Mei Niu, Yongzhen Yang, Shiping Yu, Li Zhang

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Abstract

Dressings play a crucial role in the management of burn wound. In this study, the cotton bandage was modified with the Poly (N-isopropylacrylamide) / graphite oxide/nano silver (PNIPAM/GO-Ag) hydrogel to obtain a novel dressing (PNIPAM/GO-Ag/COT). The healing effect of the PNIPAM/GO-Ag/COT dressing on deep second-degree burn wounds in rats and the changes of related inflammatory factors were explored and analyzed systematically. The deep second-degree burn model was established by the steam scald method in Sprague–Dawley (SD) rats. The granulation tissue, collagen deposition, the expressions of tumor necrosis factor-α (TNF-α), and basic fibroblast growth factor (bFGF) of the wound were evaluated by means of the HE staining, Masson staining, ELISA, and immunohistochemistry methods. The results showed that, compared with the blank group (rats without the dressing treatment), the PNIPAM/GO-Ag/COT dressing reduced the expression of TNF-α by approximately 18 % and promoted the bFGF expression in wound tissue. Compared to the control group (rats with the gauze treatment), the wound healing rate in the PNIPAM/GO-Ag/COT dressing group was 58 % on the 14th day, with an increase of 30 %. These results demonstrated that the PNIPAM/GO-Ag/COT dressing primarily promoted burn wound healing by reducing inflammatory reactions, promoting collagen deposition, and enhancing the expression of bFGF.

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负载 PNIPAM/GO-Ag 水凝胶的棉绷带对大鼠模型深二度烧伤创面的愈合机制

敷料在烧伤创面的处理中起着至关重要的作用。本研究用聚（N-异丙基丙烯酰胺）/氧化石墨/纳米银（PNIPAM/GO-Ag）水凝胶对棉绷带进行改性，得到了一种新型敷料（PNIPAM/GO-Ag/COT）。系统地探讨和分析了 PNIPAM/GO-Ag/COT 敷料对大鼠深二度烧伤创面的愈合效果以及相关炎症因子的变化。采用蒸汽烫伤法建立 Sprague-Dawley (SD) 大鼠深二度烧伤模型。通过 HE 染色法、Masson 染色法、ELISA 法和免疫组化法对创面的肉芽组织、胶原沉积、肿瘤坏死因子-α（TNF-α）和碱性成纤维细胞生长因子（bFGF）的表达进行了评价。结果表明，与空白组（未使用敷料的大鼠）相比，PNIPAM/GO-Ag/COT敷料降低了伤口组织中TNF-α的表达约18%，并促进了bFGF的表达。与对照组（使用纱布处理的大鼠）相比，PNIPAM/GO-Ag/COT 敷料组在第 14 天的伤口愈合率为 58%，提高了 30%。这些结果表明，PNIPAM/GO-Ag/COT 敷料主要通过减少炎症反应、促进胶原蛋白沉积和增强 bFGF 的表达来促进烧伤创面的愈合。

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来源期刊

Burns 医学-皮肤病学

CiteScore

4.50

自引率

18.50%

发文量

304

审稿时长

72 days

期刊介绍： Burns aims to foster the exchange of information among all engaged in preventing and treating the effects of burns. The journal focuses on clinical, scientific and social aspects of these injuries and covers the prevention of the injury, the epidemiology of such injuries and all aspects of treatment including development of new techniques and technologies and verification of existing ones. Regular features include clinical and scientific papers, state of the art reviews and descriptions of burn-care in practice. Topics covered by Burns include: the effects of smoke on man and animals, their tissues and cells; the responses to and treatment of patients and animals with chemical injuries to the skin; the biological and clinical effects of cold injuries; surgical techniques which are, or may be relevant to the treatment of burned patients during the acute or reconstructive phase following injury; well controlled laboratory studies of the effectiveness of anti-microbial agents on infection and new materials on scarring and healing; inflammatory responses to injury, effectiveness of related agents and other compounds used to modify the physiological and cellular responses to the injury; experimental studies of burns and the outcome of burn wound healing; regenerative medicine concerning the skin.