Exploring the underlying mechanism by transcriptome sequencing in rats with high-voltage electrical burns and the role of iron metabolism

IF 3.2 3区医学 Q2 CRITICAL CARE MEDICINE Burns Pub Date : 2024-08-04 DOI:10.1016/j.burns.2024.07.030

Jiawen Hao, Mengyuan Lu, Xuegang Zhao, Congying Li, Chenyang Ge, Jing Zhang, Lihong Tu, Qingfu Zhang

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Abstract

Clinically, the condition of skeletal muscle injury is the key to the process of high voltage electrical burn (HVEB) wound repair. The aim of this study was to identify the potential mechanisms and intervention targets of skeletal muscle injury after HVEB. A skeletal muscle injury model in SD rats with HVEB was made. Pathological examination and transcriptome sequencing of injured skeletal muscles were performed, and the expression levels of key proteins and genes in related signaling pathways were verified. Skeletal muscle injury was progressively aggravated within 48 h, then the injury was gradually repaired with scar formation occurring within 1 week. The mechanism of skeletal muscle injury is complex and varied, and ferroptosis is one of the mechanisms. The ferrous iron content in the injured skeletal muscle tissue of model rats increased significantly at 24 h after injury. After 24 h, damage to injured skeletal muscle tissue could be alleviated by increasing iron storage and blocking lysosomal phagocytosis of autophagy. Skeletal muscle injury caused by HVEB is characterized by progressive progression after injury. Ferroptosis is involved in the mechanism of HVEB, and iron metabolism-related proteins may be potential targets for preventing progressive skeletal muscle injury.

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通过转录组测序探索高压电烧伤大鼠的潜在机制以及铁代谢的作用

在临床上，骨骼肌损伤状况是高压电烧伤（HVEB）创面修复过程的关键。本研究旨在确定高压电烧伤后骨骼肌损伤的潜在机制和干预目标。本研究制作了一个高压电烧伤 SD 大鼠骨骼肌损伤模型。对损伤骨骼肌进行了病理学检查和转录组测序，并验证了相关信号通路中关键蛋白和基因的表达水平。骨骼肌损伤在 48 h 内逐渐加重，然后在 1 周内逐渐修复并形成瘢痕。骨骼肌损伤的机制复杂多样，而铁变态反应是其中的一种机制。损伤后 24 小时，模型大鼠骨骼肌损伤组织中的亚铁含量明显增加。24 小时后，通过增加铁储存和阻断溶酶体吞噬自噬，可减轻损伤骨骼肌组织的损伤。由 HVEB 引起的骨骼肌损伤的特点是损伤后进行性进展。铁代谢参与了 HVEB 的机理，铁代谢相关蛋白可能是预防骨骼肌进行性损伤的潜在靶点。

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来源期刊

Burns 医学-皮肤病学

CiteScore

4.50

自引率

18.50%

发文量

304

审稿时长

72 days

期刊介绍： Burns aims to foster the exchange of information among all engaged in preventing and treating the effects of burns. The journal focuses on clinical, scientific and social aspects of these injuries and covers the prevention of the injury, the epidemiology of such injuries and all aspects of treatment including development of new techniques and technologies and verification of existing ones. Regular features include clinical and scientific papers, state of the art reviews and descriptions of burn-care in practice. Topics covered by Burns include: the effects of smoke on man and animals, their tissues and cells; the responses to and treatment of patients and animals with chemical injuries to the skin; the biological and clinical effects of cold injuries; surgical techniques which are, or may be relevant to the treatment of burned patients during the acute or reconstructive phase following injury; well controlled laboratory studies of the effectiveness of anti-microbial agents on infection and new materials on scarring and healing; inflammatory responses to injury, effectiveness of related agents and other compounds used to modify the physiological and cellular responses to the injury; experimental studies of burns and the outcome of burn wound healing; regenerative medicine concerning the skin.