Extraction of phenolic compounds from Acinos rotundifolius pers. And evaluation of bioactivities

IF 1.4 4区生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY Biochemical Systematics and Ecology Pub Date : 2024-07-29 DOI:10.1016/j.bse.2024.104876

Raziye Ozturk Urek , Aylin Oner

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Abstract

The aim of the present study was to determine the phenolic profile, antioxidant capacities, inhibition of enzymes related to dermatological and neurodegenerative diseases, and cytotoxic properties of Acinos rotundifolius Pers. For this purpose, three different extraction methods were applied: Soxhlet extraction, ultrasonic-assisted extraction (UAE), and infusion (INF). The use of different solvents (ethyl acetate, methanol, water) has also been applied. The RP-HPLC-DAD data highlighted that Soxhlet extraction in MeOH/water is more effective at extracting flavonoids and phenolic acids. The UAE extract, which was richer in chlorogenic acid, exhibited a better scavenging capacity versus HO^•, NO^• and ABTS^•+ were determined by UAE, whereas the one obtained via Soxhlet extraction in water had a better efficacy to eliminate O₂^•. The greatest inhibitory effects on AChE and tyrosinase enzymes were demonstrated by the infusion. The different composition of the extracts obtained with the different methods also influences the cytotoxic properties which were investigated by MTT test on HepG2 and OE-33 cell lines.

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从 Acinos rotundifolius pers.生物活性评估

本研究旨在确定莹石的酚类成分、抗氧化能力、对皮肤病和神经退行性疾病相关酶的抑制作用以及细胞毒性。为此，采用了三种不同的提取方法：索氏提取法、超声波辅助提取法（UAE）和浸泡法（INF）。此外，还使用了不同的溶剂（乙酸乙酯、甲醇、水）。RP-HPLC-DAD 数据表明，用 MeOH/ 水进行索氏提取能更有效地提取黄酮类化合物和酚酸。阿联酋提取物含有更丰富的绿原酸，对 HO、NO 和 ABTS 的清除能力更强，而在水中索氏提取的提取物对 O 的清除能力更强。用不同方法提取的提取物的不同成分也会影响其细胞毒性特性，这些特性是通过对 HepG2 和 OE-33 细胞系进行 MTT 试验来研究的。

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来源期刊

Biochemical Systematics and Ecology 生物-进化生物学

CiteScore

3.00

自引率

12.50%

发文量

147

审稿时长

43 days

期刊介绍： Biochemical Systematics and Ecology is devoted to the publication of original papers and reviews, both submitted and invited, in two subject areas: I) the application of biochemistry to problems relating to systematic biology of organisms (biochemical systematics); II) the role of biochemistry in interactions between organisms or between an organism and its environment (biochemical ecology). In the Biochemical Systematics subject area, comparative studies of the distribution of (secondary) metabolites within a wider taxon (e.g. genus or family) are welcome. Comparative studies, encompassing multiple accessions of each of the taxa within their distribution are particularly encouraged. Welcome are also studies combining classical chemosystematic studies (such as comparative HPLC-MS or GC-MS investigations) with (macro-) molecular phylogenetic studies. Studies that involve the comparative use of compounds to help differentiate among species such as adulterants or substitutes that illustrate the applied use of chemosystematics are welcome. In contrast, studies solely employing macromolecular phylogenetic techniques (gene sequences, RAPD studies etc.) will be considered out of scope. Discouraged are manuscripts that report known or new compounds from a single source taxon without addressing a systematic hypothesis. Also considered out of scope are studies using outdated and hard to reproduce macromolecular techniques such as RAPDs in combination with standard chemosystematic techniques such as GC-FID and GC-MS.