Biochemical Systematics and Ecology最新文献

Does Hesperozygis ringens (Benth.) Epling essential oil have potential for control of Conyza bonariensis (L.) Cronquist without damaging native species? Hesperozygis（下）桉树精油具有防治bonyza bonariensis的潜力。不损害本地物种的克朗奎斯特？

IF 2 4区生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY

Biochemical Systematics and Ecology

Pub Date : 2026-01-29 DOI: 10.1016/j.bse.2026.105231

Fernanda Bruxel , Amanda Pastório Borges , Amanda Janner Marques , Marcos Vinicius Vizioli Klaus , Mara Cíntia Winhelmann , Alex Dapont , Camila Thais Scheibler , Shirley Martins Silva , Elisete Maria de Freitas

Essential oils (EO) are volatile plant substances that can be used in the development of natural herbicides, offering an alternative that is less harmful to the environment. This study investigated the phytotoxicity of Hesperozygis ringens essential oil and its major compound, pulegone, on Conyza bonariensis, an infesting plant, and Plantago australis, native species. H. ringens leaves were collected and the EO was extracted by hydro-distillation. The concentrations of 0, 0.001, 0.0025, 0.005, 0.01, and 0.02 % of the EO were tested in bioassays of both germination and early vegetative development, as well as 0, 1.0 % in adult plants. The experiments were conducted on Petri dishes and glass flasks, under controlled conditions. An analysis of variance (ANOVA) was used to evaluate the data. The EO contained 29 compounds, and pulegone was the major compound. The concentrations of 0.01 and 0.02 % inhibited germination and affected early development, causing damages to the membranes, especially in C. bonariensis. Pulegone also had toxicity, yet less intense than the EO, thus suggesting that the synergy of compounds is responsible for the toxic activity. In adult plants, EO toxicity was observed starting at 24 h, reaching its maximum within 72 h. The results confirmed the toxic activity of the EO, indicating that H. ringens is a promising source for natural herbicides.

精油（EO）是一种挥发性植物物质，可用于开发天然除草剂，提供了一种对环境危害较小的替代品。本研究研究了海橘精油及其主要化合物普乐酮对有害植物bonariensis （Conyza bonariensis）和本土植物Plantago australis（车前草）的植物毒性。收集红豆杉叶片，采用水蒸气蒸馏法提取黄酮。在萌发和早期营养发育的生物测定中分别检测了0、0.001、0.0025、0.005、0.01和0.02%的EO浓度，在成年植物中检测了0、1.0%的EO浓度。实验在培养皿和玻璃烧瓶上进行，在受控条件下进行。采用方差分析（ANOVA）对数据进行评价。其中含有29种化合物，以普列酮为主要化合物。0.01%和0.02%的浓度抑制了种子的萌发，影响了早期发育，造成了膜的损伤，特别是在bonariensis中。Pulegone也有毒性，但没有EO那么强烈，因此表明化合物的协同作用是毒性活性的原因。在成虫植株中，从24 h开始观察到EO的毒性，在72 h内达到最大，结果证实了EO的毒性活性，表明草根草是一种很有前景的天然除草剂来源。

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引用次数: 0

Untargeted metabolomics profiling of Ajuga L. species (Lamiaceae): Comprehensive workflow and chemodiversity assessment using FTIR, QTRAP LC-MS/MS fingerprinting, and molecular networking 利用FTIR、QTRAP LC-MS/MS指纹图谱和分子网络技术，综合工作流程和化学多样性评估麻草属植物（Lamiaceae）的非靶向代谢组学分析

IF 2 4区生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY

Biochemical Systematics and Ecology

Pub Date : 2026-01-28 DOI: 10.1016/j.bse.2026.105232

Navaz Kharazian , Chun Lei Zhiang

The genus Ajuga L. (Lamiaceae), known for its diverse morphological traits and established medicinal properties, comprises over 300 species worldwide. Despite its pharmacological relevance, inter- and intraspecific metabolomic and chemodiversity data remain limited. This study employed a comprehensive, untargeted metabolomics approach that integrated FTIR and QTRAP LC–MS/MS with molecular networking to address this knowledge gap. Twenty-five accessions, representing two species, three subspecies, and two varieties, were collected from natural habitats. The dried aerial parts were extracted using 98 % methanol and then processed via ultrasonic homogenization. An automated workflow facilitated compound annotation, biomarker identification, intraspecific variation analysis, chemotype identification, and molecular networking using MetaboAnalyst 6.0 and the GNPS platform. FTIR profiling revealed dominant vibrational bands associated with O–H, N–H, C–H, CO, CC, C–O, and C–N stretching, as well as N–H and C–H bending. Classical molecular networking via the GNPS platform annotated 261 molecular features, including 139 metabolites that had not been previously reported in Ajuga. A total of 77 validated biomarkers and 17 intraspecific chemotypes with distinct taxonomic significance were identified. Sixty-nine molecular networks were constructed, displaying extensive subcluster diversity and rich chemical variability. The integrated analytical pipeline combining LC-MS/MS and FTIR fingerprinting with multivariate and automated workflows effectively discriminated Ajuga taxa. The chemodiversity uncovered in thi study provides essential data for future phytochemical and pharmacological investigations.

麻草属（Lamiaceae）以其多样的形态特征和已建立的药用特性而闻名，在世界范围内共有300多个物种。尽管其药理相关性，种间和种内代谢组学和化学多样性数据仍然有限。本研究采用了一种全面的非靶向代谢组学方法，将FTIR和QTRAP LC-MS /MS与分子网络相结合，以解决这一知识差距。在自然生境中收集了2种、3个亚种和2个变种共25份资料。干燥后的空气部件用98%甲醇提取，超声均质处理。使用MetaboAnalyst 6.0和GNPS平台，自动化工作流程促进了化合物注释、生物标志物鉴定、种内变异分析、化学型鉴定和分子网络。FTIR分析显示，主要的振动带与O-H、N-H、C-H、CO、CC、C-O和C-N拉伸以及N-H和C-H弯曲有关。通过GNPS平台的经典分子网络注释了261个分子特征，包括139个以前未在Ajuga报道过的代谢物。共鉴定出77个经验证的生物标志物和17个具有不同分类意义的种内化学型。构建了69个分子网络，具有广泛的亚簇多样性和丰富的化学变异性。结合LC-MS/MS和FTIR指纹图谱的综合分析流水线，采用多元自动化的工作流程，有效地区分了黄花草的分类。本研究发现的化学多样性为未来的植物化学和药理学研究提供了重要的数据。

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引用次数: 0

Ultrasound-assisted extraction and HPLC-PDA/ESI-MS profiling of Teucrium gypsophilum: Antioxidant and enzyme inhibitory activities 超声辅助提取及HPLC-PDA/ESI-MS分析：抗氧化和酶抑制活性

IF 2 4区生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY

Biochemical Systematics and Ecology

Pub Date : 2026-01-28 DOI: 10.1016/j.bse.2026.105233

Taoufiq Benali , Rhizlan Abdnim , Mustapha Laghmari , Jihane Touhtouh , Amal Elrherabi , Fatima Lakhdar , Abdelmajid Khabbach , Mohamed Bnouham , Aziz Bouymajane , Balahbib Abdelaali , Abdelhakim Bouyahya , Gokhan Zengin , Roberto Laganà Vinci , Francesco Cacciola , Khalil Hammani

Teucrium gypsophilum, an endemic Moroccan species traditionally used for medicinal purposes, was investigated for the first time for its phenolic profile and biological activities. Ethanolic, aqueous, and methanolic leaf extracts were prepared via ultrasound-assisted extraction and analyzed using HPLC-PDA/ESI-MS. The main phenolic chemicals found in the methanolic extract were leonoside A/alyssonoside isomer (96.37 ± 0.361 mg/g), caffeoylquinic acid (75.66 ± 0.218 mg/g), and verbascoside (14.14 ± 0.130 mg/g). Regarding the ethanolic extract, chemical analysis identified the leonoside A/alyssonoside isomer as the most abundant (59.52 ± 0.154 mg/g), followed by the verbascoside isomer and the dihexoside isomer of apigenin with 8.89 ± 0.102 and 5.39 ± 0.005 mg/g, respectively. For aqueous extract, the main compound was caffeic acid 12.65 ± 0.105 mg/g, followed by echinacoside isomer with 6.89 ± 0.006 mg/g.

The extracts demonstrated significant antioxidant activity, with the methanolic extract exhibiting the highest DPPH scavenging effect (IC₅₀ = 2.64 ± 0.52 μg/mL) and reducing power (15.66 ± 0.11 mg AAE/g). In vitro enzyme assays revealed dose-dependent inhibitory effects against elastase (IC₅₀: 9.09 ± 0.003 μg/mL), phospholipase A₂ (IC₅₀: 31.75 ± 0.0005 μg/mL, α-amylase (IC₅₀: 0.45 ± 0.004 μg/mL), anti-urease, and anti- α -glucosidase effect which IC₅₀ values equal 0.602 ± 0.049 and 0.604 ± 0.084 mg/mL and 0.38 ± 0.005 μg/mL, respectively. These findings highlight the pharmaceutical and nutraceutical potential of T. gypsophilum as a rich source of bioactive phenolic compounds.

Teucrium gypsophilum是一种摩洛哥特有的传统药用植物，首次对其酚类特征和生物活性进行了研究。采用超声辅助提取法分别制备乙醇、水、甲醇叶提取物，并采用HPLC-PDA/ESI-MS进行分析。甲醇提取物中主要酚类化学成分为leonoside A/alyssonoside异构体（96.37±0.361 mg/g）、咖啡酰奎宁酸（75.66±0.218 mg/g）和毛蕊花苷（14.14±0.130 mg/g）。乙醇提取物中leonoside A/alyssonoside异构体含量最多（59.52±0.154 mg/g），其次是毛蕊花苷异构体（8.89±0.102 mg/g），其次是芹菜素二已苷异构体（5.39±0.005 mg/g）。水提物的主要成分为咖啡酸（12.65±0.105 mg/g），其次为紫锥花苷异构体（6.89±0.006 mg/g）。其中甲醇提取物对DPPH的清除率最高（IC50 = 2.64±0.52 μg/mL），还原力最高（15.66±0.11 mg AAE/g）。体外酶活性测定显示其对弹性酶（IC50: 9.09±0.003 μg/mL）、磷脂酶A2 （IC50: 31.75±0.0005 μg/mL）、α-淀粉酶（IC50: 0.45±0.004 μg/mL）、抗脲酶和抗α-葡萄糖苷酶的抑制作用呈剂量依赖性，IC50分别为0.602±0.049和0.604±0.084 mg/mL和0.38±0.005 μg/mL。这些发现突出了石膏霉作为生物活性酚类化合物的丰富来源的制药和营养保健潜力。

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引用次数: 0

Chemical constituents from the rhizomes of Abelmoschus manihot (L.) and their chemotaxonomic significance 麻豆根状茎化学成分及其化学分类意义

IF 2 4区生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY

Biochemical Systematics and Ecology

Pub Date : 2026-01-21 DOI: 10.1016/j.bse.2026.105234

Ruiqing Wu , Yuhuan Sun , Shaoshan Gao , Shumeng Guo , Zhaoyang Lin , Changjiang Ying , Yan Li

Phytochemical investigation on the rhizomes of Abelmoschus manihot (L.) has resulted in the isolation of 24 natural compounds including six phenolics (1–3, 5, 17 and 19), one sesquiterpenoid (4), three coumarins (7–9), three alkaloids (6, 11 and 12), one lignan (10), two nucleosides (13 and 14), three terpenoids (15, 18 and 20), one steroid (16) and four flavonoids (21–24) from its ethanolic extract using column chromatography. Their structures were determined by analysis of NMR spectra and comparison of the data with literature. Among them, nine compounds (1, 5, 9–12, 17, 19 and 20) were firstly found in the family Malvaceae; and nine compounds (2, 4, 6–8, 15, 18, 21 and 22) were isolated for the first time from A. manihot (L.). Their chemotaxonomic significance was comprehensively discussed herein.

利用柱层析技术对麻豆根状茎进行了植物化学研究，从其乙醇提取物中分离出24种天然化合物，包括6种酚类化合物（1 - 3,5,17和19），1种倍半萜(4)，3种香豆素（7-9），3种生物碱（6,11和12），1种木脂素（10），2种核苷（13和14），3种萜类（15,18和20），1种类固醇（16）和4种黄酮类（21-24）。通过核磁共振谱分析和文献资料对比确定了它们的结构。其中化合物1、5、9 ~ 12、17、19、20为首次在锦葵科中发现；其中9个化合物（2、4、6 ~ 8、15、18、21和22）为首次从马尼乌特草中分离得到。本文全面讨论了它们的化学分类意义。

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引用次数: 0

Metabolomic discrimination of the Primula auricula genetic complex 报春花遗传复合体的代谢组学鉴定

IF 2 4区生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY

Biochemical Systematics and Ecology

Pub Date : 2026-01-21 DOI: 10.1016/j.bse.2026.105230

Stéphanie Morelon , Gaëtan Glauser , Emmanuel Defossez , Jérémy Gauthier , Jason Grant , Nadir Alvarez , Sergio Rasmann

Understanding how evolutionary history and ecological pressures shape plant chemical diversity is central to ecology and evolution, yet it remains unclear whether metabolomic data can reliably detect fine-scale, intraspecific divergence, particularly in morphologically cryptic taxa. While metabolomics has revealed broad patterns of chemical evolution across lineages, its power to resolve genetically structured variation within species is still underexplored. Here, we investigated the alpine Primula auricula complex, a morphologically uniform but genetically subdivided taxon distributed across the Alps. Using ultra-high-performance liquid chromatography coupled with mass spectrometry, we profiled the metabolomes of individuals sampled from 37 populations spanning three main genetic clades and an outlier group, previously identified through ddRADseq phylogeography. We found that metabolomic diversity carries a strong phylogenetic signal: each clade exhibited distinct chemical profiles, with exclusive or enriched metabolite superclasses such as carotenoids in one clade and phenylpropanoids in another. Outlier populations displayed reduced metabolomic richness, consistent with potential genetic drift or bottlenecks. While phylogenetic structure was the dominant driver of chemical variation, climatic variables, particularly temperature and precipitation, modulated certain stress-related metabolite groups, such as octadecanoids. Our results demonstrate that metabolomic profiling can capture both historical divergence and ecological adaptation in cryptic alpine taxa. By linking chemical, genetic, and environmental variation, this study highlights metabolomics as a cost-effective and high-resolution approach to uncover cryptic diversity, refine taxonomy, and inform conservation strategies in biodiversity hotspots increasingly threatened by climate change.

了解进化历史和生态压力如何塑造植物化学多样性是生态学和进化的核心，但代谢组学数据是否能够可靠地检测精细尺度的种内差异，特别是在形态隐秘的分类群中，尚不清楚。虽然代谢组学揭示了跨谱系化学进化的广泛模式，但其解决物种内遗传结构变异的能力仍未得到充分探索。本文研究了分布在阿尔卑斯山脉的高山报春花复合体（Primula auricula complex），这是一个形态统一但遗传细分的分类群。利用超高效液相色谱联用质谱，我们分析了37个种群的个体代谢组，这些种群跨越了三个主要的遗传分支和一个异常组，之前通过ddRADseq系统地理学鉴定。我们发现，代谢组学多样性携带着一个强大的系统发育信号：每个进化支都表现出不同的化学特征，具有独特或丰富的代谢物超类，如一个进化支中的类胡萝卜素和另一个进化支中的苯丙素。异常群体显示代谢组丰富度降低，与潜在的遗传漂变或瓶颈一致。虽然系统发育结构是化学变化的主要驱动因素，但气候变量，特别是温度和降水，调节某些与应激相关的代谢物群，如十八烷酸。我们的研究结果表明，代谢组学分析可以捕捉到高山隐蔽分类群的历史分化和生态适应。通过将化学、遗传和环境变化联系起来，本研究强调了代谢组学作为一种高成本效益和高分辨率的方法，可以在日益受到气候变化威胁的生物多样性热点地区发现隐藏的多样性，完善分类，并为保护策略提供信息。

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引用次数: 0

Chemical constituents from the heartwood of Dalbergia odorifera and their chemotaxonomic significance 降香黄檀心材化学成分及其化学分类意义

IF 2 4区生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY

Biochemical Systematics and Ecology

Pub Date : 2026-01-17 DOI: 10.1016/j.bse.2026.105228

Xiaohui Gong, Xiaoman Li, Yingyi Wang, Feng Shao

Forty-one compounds, including 6 pterocarpans(4, 6–10), 6 neoflavonoids(28–33), 2 dihydroflavones(11, 12), 7 dihydroisoflavones(13–19), 4 isoflavones(20–23), 3 isoflavans(24–26), 1 chalcone(27), 4 terpenoids(1–3, 40), 3 benzofurans(36, 37, 41), 1 coumarin(5), and 4 phenols(34, 35, 38, 39) were obtained from the heartwood of Dalbergia odorifera. Their structures were elucidated by a combination of Nuclear Magnetic Resonance (NMR) Spectroscopy, Mass Spectrometry (MS) and comparison with the literature. Among them, compounds 35 and 38 were reported from the Leguminosae family for the first time. Compounds 6, 8, 24, and 34 represent discoveries for the first time within the genus Dalbergia, while compound 41 was found in Dalbergia odorifera for the first time. Moreover, the chemotaxonomic significance of these compounds was summarized.

从降香黄檀心材中共分离得到6个萜类化合物（4,6 - 10）、6个新黄酮类化合物（28-33）、2个二氢黄酮类化合物（11,12）、7个二氢异黄酮类化合物（13-19）、4个异黄酮类化合物（20-23）、3个异黄酮类化合物（24-26）、1个查尔酮类化合物（27）、4个萜类化合物（1 - 3,40）、3个苯并呋喃类化合物（36,37,41）、1个香豆素类化合物(5)和4个酚类化合物（34,35,38,39）。通过核磁共振（NMR）、质谱（MS）和文献比对等方法对其结构进行了鉴定。其中化合物35和38为首次从豆科植物中分离得到。其中化合物6、8、24、34为首次从黄檀属植物中发现，化合物41为首次从降香黄檀中发现。并对这些化合物的化学分类意义进行了综述。

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引用次数: 0

A new sesquiterpene and other constituents from Dendrobium nobile Lindl. and their chemotaxonomic significance 从石斛中分离出一种新的倍半萜和其他成分。以及它们的化学分类意义

IF 2 4区生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY

Biochemical Systematics and Ecology

Pub Date : 2026-01-16 DOI: 10.1016/j.bse.2026.105229

Wenyuan Zhou , Yi Yang , Xiaonan Li , Xiujuan Fu , Siwei Chen , Hui Lei , Ying Zhang , Dan Zhang , Chunlian Wu

Dendrobium nobile Lindl. belongs to the Orchidaceae family and is used in China as a herbal tea. It is widely utilized in traditional Chinese medicine to treat various ailments. In this study, six compounds were identified from the stems of Dendrobium nobile through the combined use of TLC and CC, including dendrosideol A (1), as well as five known compounds (2–6). The structures of these compounds were determined by 1D and 2D NMR spectra (¹H NMR, ¹³C NMR, HSQC, HMBC, COSY and NOESY), and HR-ESI-MS spectroscopic analysis and the absolute configuration was confirmed by ECD calculation. In addition, the chemotaxonomic significance of these compounds was discussed.

石斛。属于兰科，在中国被用作凉茶。它在中医中被广泛用于治疗各种疾病。本研究通过TLC和CC相结合的方法，从石斛茎中鉴定出6种化合物，包括石斛苷醇A(1)，以及5种已知化合物（2-6）。通过1H NMR、13C NMR、HSQC、HMBC、COSY和NOESY等一维和二维NMR谱和HR-ESI-MS谱分析确定了化合物的结构，并通过ECD计算确定了化合物的绝对构型。此外，还讨论了这些化合物的化学分类意义。

引用次数: 0

Chemical constituents from the n-Butanol extract of Senecio scandens Buch. - Ham and their chemotaxonomic significance 香参正丁醇提取物的化学成分研究。-火腿及其化学分类意义

IF 2 4区生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY

Biochemical Systematics and Ecology

Pub Date : 2026-01-15 DOI: 10.1016/j.bse.2026.105226

Chunshan Ke, Liang Lai, Chuanqi Xie, Junwei Xu, Zhiyong Xu, Yunfei Liu, Yongsheng Guo, Xiaodan Han, Wei Xiong, Lei Wu, Hongxu Li

The phytochemical investigation of Senecio scandens Buch. - Ham extracted from n-BuOH resulted in the isolation and identification of eight known compounds—two phenolic acids (1–2) and six flavonoid compounds (3–8). The structures of these compounds were elucidated using spectroscopic methods, and their nuclear magnetic resonance spectra were compared with previously reported data in the literature. This study reports, for the first time, the isolation of compounds 2 and 7 from the Asteraceae family, compound 1 from the genus Senecio, and compounds 5 and 8 from the species S. scandens Buch. - Ham. Furthermore, the chemotaxonomic significance of the compounds isolated from S. scandens Buch. -Ham was examined in detail.

山参的植物化学研究。从正丁醇中提取火腿，分离鉴定出8种已知化合物——2种酚酸（1-2）和6种黄酮类化合物（3-8）。用波谱方法对这些化合物的结构进行了鉴定，并将其核磁共振波谱与文献中报道的数据进行了比较。本研究首次从菊科植物中分离得到化合物2和7，化合物1从菊科植物中分离得到，化合物5和8从菊科植物S. scandens Buch中分离得到。——火腿。此外，还研究了从香参中分离得到的化合物的化学分类意义。-哈姆被详细检查过。

引用次数: 0

Phytochemical investigation of Vernonia amygdalina Delile 苦杏仁的植物化学研究

IF 2 4区生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY

Biochemical Systematics and Ecology

Pub Date : 2026-01-14 DOI: 10.1016/j.bse.2026.105223

Pham Thi Bich Dao , Le Ba Vinh , Nguyen Tien Dat , Ngo Van Hieu , Chu Van Tan , Pham Van Cong , Nguyen Thi Thanh , Nguyen Hai Dang , Hoang Le Tuan Anh

Phytochemical investigation of the methanol extracts from the stems and leaves of Vernonia amygdalina Delile afforded one new stigmastane steroidal saponin, named 28-epi-vernoramyoside D (1), together with five known analogues: vernoramyoside D (2), (22R,23S,24R,28S)-28-methoxy-7,8,9,11-tetradehydro-3β,16α,21,24-tetrahydroxy 21,23:22,28-diepoxy-5α-stigmastane (3), vernoamyoside E (4), veramyoside H (5), and veramyoside J (6). The structures of all isolated compounds were elucidated by comprehensive 1D and 2D NMR spectroscopy, along with HR-ESI-MS analysis. Compounds 1–6 were evaluated for their inhibitory activities against α-glucosidase and xanthine oxidase. Among them, compounds 5 and 6 exhibited significant α-glucosidase inhibition, with IC₅₀ values of 48.55 ± 4.31 μM and 7.42 ± 0.95 μM, respectively, comparable to that of acarbose (127.53 ± 1.73 μM). None of the compounds showed inhibitory activity against xanthine oxidase. These findings highlight the chemotaxonomic significance of the isolated constituents and their potential as α-glucosidase inhibitory agents.

对苦扁桃茎叶甲醇提取物进行植物化学研究，得到了一种新的苦扁桃烷甾体皂苷，命名为28-epi-苦扁桃苷D(1)，以及五种已知的类似物：苦扁桃苷D (2), (22R,23S,24R,28S)-28-甲氧基-7,8,9,11-四氢-3β，16α,21,24-四羟基21,23:22,28-二氧基-5α-苦扁桃苷(3)，苦扁桃苷E(4)，苦扁桃苷H(5)和苦扁桃苷J(6)。所有分离化合物的结构都通过1D和2D NMR以及HR-ESI-MS分析得到。测定化合物1 ~ 6对α-葡萄糖苷酶和黄嘌呤氧化酶的抑制活性。其中化合物5和6对α-葡萄糖苷酶的抑制作用显著，IC50值分别为48.55±4.31 μM和7.42±0.95 μM，与阿卡波糖的IC50值（127.53±1.73 μM）相当。这些化合物对黄嘌呤氧化酶均无抑制作用。这些发现突出了分离成分的化学分类意义及其作为α-葡萄糖苷酶抑制剂的潜力。

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引用次数: 0

Discrimination of various botanical origins of Lasiosphaera calvatia and their adulterant by chemical fingerprint, multi-component quantification, and chemometric analysis 用化学指纹图谱、多组分定量和化学计量分析方法鉴别不同植物来源的牛蹄草及其伪品

IF 2 4区生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY

Biochemical Systematics and Ecology

Pub Date : 2026-01-14 DOI: 10.1016/j.bse.2026.105227

Hong Sun , Yanjuan Zhai , Jiafei Li , Xingyu Guo , Jiayi Wang , Chao Gu , Yujing Zhang , Song Li , Shengjun Chen , Chaofeng Zhang , Dali Kang , Yuntian Zhang

Lasiosphaera calvatia, a macrofungus from the Lycoperdaceae family, is a famous traditional Chinese medicine with various pharmacological activities, but its chemotaxonomy investigation is relatively insufficient. In addition to the three official origins recorded in the Pharmacopeia of the People's Republic of China: Lasiosphaera fenzlii, Calvatia gigantea, and Calvatia lilacina, there are other related species and adulterants in the market, such as Bovistella sinensis, and it is necessary to establish effective methods for species discrimination. To achieve this task, High-performance liquid chromatography (HPLC) fingerprint method were developed, and a total of 19 characteristic peaks were established. Combined with chemometric analysis, including cluster analysis (CA), principal component analysis (PCA), and orthogonal partial least squares-discriminant analysis (OPLS-DA), the four species were effectively discriminated. Simultaneously, 6 components (α-linolenic acid, linoleic acid, palmitic acid, oleic acid, stearic acid, and ergosterol) were determined, and the content analysis proposed them as characteristic markers for species discrimination. The above results are integrated to indicate that the quality of Calvatia lilacina and Calvatia gigantea is relatively high, and Bovistella sinensis is the lowest. The established methods are durable and reproducible, providing a foundation for quality evaluation and identification of this medicinal material.

番茄科大型真菌Lasiosphaera calvatia是一种著名的中药，具有多种药理活性，但其化学分类学研究相对不足。除了《中华人民共和国药典》记载的三种正式种源：Lasiosphaera fenzlii、calvara gigantea和calvara lilacina外，市场上还有其他相关种源和掺假种源，如Bovistella sinensis，需要建立有效的种源鉴别方法。为此，建立了高效液相色谱（HPLC）指纹图谱，共建立了19个特征峰。结合聚类分析（CA）、主成分分析（PCA）和正交偏最小二乘判别分析（OPLS-DA）等化学计量学分析，对4种植物进行了有效的鉴别。同时，测定了α-亚麻酸、亚油酸、棕榈酸、油酸、硬脂酸、麦角甾醇6种成分，并通过含量分析提出了6种成分可作为物种鉴别的特征标记。综合以上结果可知，丁香牛膝和大牛膝质量较高，中华牛膝质量最低。所建立的方法耐用、重复性好，为该药材的质量评价和鉴定提供了依据。

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引用次数: 0