Synthesis of Chi-sphere silver nanocomposite and nanocomposites of silver, gold, and S@G using a chitosan biopolymer extracted from potato peels and their antimicrobial application
{"title":"Synthesis of Chi-sphere silver nanocomposite and nanocomposites of silver, gold, and S@G using a chitosan biopolymer extracted from potato peels and their antimicrobial application","authors":"","doi":"10.1016/j.nanoso.2024.101297","DOIUrl":null,"url":null,"abstract":"<div><p>Chitin and chitosan have been proven to have numerous applications in biomedical, pharmaceutical, and industrial fields. In the present study, chitin structural polymer was extracted from potato peel wastes by the use of chemical methods and chitosan biopolymer was generated through the deacetylation of the isolated potato peel chitin. On a dry weight basis, the yield of chitin content of the potato peel wastes is 49.80±1.2 % and the yield of deacetylated potato peel chitin (potato peel chitosan) is 47.60±1.8 %. It was characterized by FTIR and <sup>1</sup>H NMR studies. Consequently, the potato peel chitosan was employed in the synthesis of Chitosan-Silver-Nanocomposite-Sphere (Chi-SNC-Sphere), and other metal nanocomposite of silver (C.g-CSNCs), gold (C.g-CGNCs), and bimetallic (C.g-CS@GNCs). The physicochemical features of the resulting products were characterized using UV-Vis, FT-IR, PXRD, FESEM, TEM, and EDAX. The FESEM unveiled a smooth, spherical, and nonporous surface morphology of the synthesized Chi-SNCs-Sphere, while the remaining three have surfaces that appeared in the form of flakes. As per TEM images, particles were visible in black-spherical (C.g-CSNC) and black-multi-shapes (C.g-CSNC, C.g-CS@GNC) nanostructures. Their sizes were confined within the range of 1–10 nm, with average values of 4.36 nm±0.40–5.85 nm±0.42. On the basis of BET analysis, C.g.CSNCs C.g-CS@GNC and C.g.CGNCs under identical conditions possess the surface area of 69.92 m<sup>2</sup>g<sup>−1,</sup> 52.35 m<sup>2</sup>g<sup>−1</sup>, and 49.75 m<sup>2</sup>g<sup>−1</sup> respectively. The nanomaterials were found stable as revealed by the study of zeta potential which is found in the range of +41.3–56.2. The results of the bioassay for antibacterial activity against <em>P. aeruginosa</em> and <em>S. aureus</em> uncovered that the Chi-SNCs-Sphere, C.g-CSNCs, and C.g-CS@GNCs demonstrated superior activities than C.g-CGNCs. According to statistical analysis results of one-way ANOVA of antibacterial results against <em>S. aureus</em>, the P-value F- calculated F-critical was 0.99,1.5 and 3.4 and for <em>P. aeruginosa</em>; P-value, F-calculated, F-critical was 0.80, 0.22 and 5.94 respectively. Minimum inhibitory concentration (MIC) was found most significant for C.g-CSNC (20 µg/mL) due to its higher surface area. These activities could be attributed to the synergistic effect of the metal nanoparticles, potato peel chitosan, and the plant extract that was used for the reduction, stabilization, and biofunctionalization.</p></div>","PeriodicalId":397,"journal":{"name":"Nano-Structures & Nano-Objects","volume":null,"pages":null},"PeriodicalIF":5.4500,"publicationDate":"2024-08-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Nano-Structures & Nano-Objects","FirstCategoryId":"1","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2352507X24002087","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"Physics and Astronomy","Score":null,"Total":0}
引用次数: 0
Abstract
Chitin and chitosan have been proven to have numerous applications in biomedical, pharmaceutical, and industrial fields. In the present study, chitin structural polymer was extracted from potato peel wastes by the use of chemical methods and chitosan biopolymer was generated through the deacetylation of the isolated potato peel chitin. On a dry weight basis, the yield of chitin content of the potato peel wastes is 49.80±1.2 % and the yield of deacetylated potato peel chitin (potato peel chitosan) is 47.60±1.8 %. It was characterized by FTIR and 1H NMR studies. Consequently, the potato peel chitosan was employed in the synthesis of Chitosan-Silver-Nanocomposite-Sphere (Chi-SNC-Sphere), and other metal nanocomposite of silver (C.g-CSNCs), gold (C.g-CGNCs), and bimetallic (C.g-CS@GNCs). The physicochemical features of the resulting products were characterized using UV-Vis, FT-IR, PXRD, FESEM, TEM, and EDAX. The FESEM unveiled a smooth, spherical, and nonporous surface morphology of the synthesized Chi-SNCs-Sphere, while the remaining three have surfaces that appeared in the form of flakes. As per TEM images, particles were visible in black-spherical (C.g-CSNC) and black-multi-shapes (C.g-CSNC, C.g-CS@GNC) nanostructures. Their sizes were confined within the range of 1–10 nm, with average values of 4.36 nm±0.40–5.85 nm±0.42. On the basis of BET analysis, C.g.CSNCs C.g-CS@GNC and C.g.CGNCs under identical conditions possess the surface area of 69.92 m2g−1, 52.35 m2g−1, and 49.75 m2g−1 respectively. The nanomaterials were found stable as revealed by the study of zeta potential which is found in the range of +41.3–56.2. The results of the bioassay for antibacterial activity against P. aeruginosa and S. aureus uncovered that the Chi-SNCs-Sphere, C.g-CSNCs, and C.g-CS@GNCs demonstrated superior activities than C.g-CGNCs. According to statistical analysis results of one-way ANOVA of antibacterial results against S. aureus, the P-value F- calculated F-critical was 0.99,1.5 and 3.4 and for P. aeruginosa; P-value, F-calculated, F-critical was 0.80, 0.22 and 5.94 respectively. Minimum inhibitory concentration (MIC) was found most significant for C.g-CSNC (20 µg/mL) due to its higher surface area. These activities could be attributed to the synergistic effect of the metal nanoparticles, potato peel chitosan, and the plant extract that was used for the reduction, stabilization, and biofunctionalization.
期刊介绍:
Nano-Structures & Nano-Objects is a new journal devoted to all aspects of the synthesis and the properties of this new flourishing domain. The journal is devoted to novel architectures at the nano-level with an emphasis on new synthesis and characterization methods. The journal is focused on the objects rather than on their applications. However, the research for new applications of original nano-structures & nano-objects in various fields such as nano-electronics, energy conversion, catalysis, drug delivery and nano-medicine is also welcome. The scope of Nano-Structures & Nano-Objects involves: -Metal and alloy nanoparticles with complex nanostructures such as shape control, core-shell and dumbells -Oxide nanoparticles and nanostructures, with complex oxide/metal, oxide/surface and oxide /organic interfaces -Inorganic semi-conducting nanoparticles (quantum dots) with an emphasis on new phases, structures, shapes and complexity -Nanostructures involving molecular inorganic species such as nanoparticles of coordination compounds, molecular magnets, spin transition nanoparticles etc. or organic nano-objects, in particular for molecular electronics -Nanostructured materials such as nano-MOFs and nano-zeolites -Hetero-junctions between molecules and nano-objects, between different nano-objects & nanostructures or between nano-objects & nanostructures and surfaces -Methods of characterization specific of the nano size or adapted for the nano size such as X-ray and neutron scattering, light scattering, NMR, Raman, Plasmonics, near field microscopies, various TEM and SEM techniques, magnetic studies, etc .