Proteomics characterization of biofilm formation by salt-tolerant Schwannyomyces etchellsii in seawater-based growth medium

IF 3.6 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Current Research in Biotechnology Pub Date : 2024-01-01 DOI:10.1016/j.crbiot.2024.100242
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Abstract

Microbial biofilms are complex communities with cells embedded in an extracellular matrix. We previously discovered that salt-tolerant yeasts Debaryomyces fabryi, Schwanniomyces etchellsii, Schwanniomyces polymorphus and Kluyveromyces marxianus were able to form biofilms when grown in seawater- but not in freshwater-based media. The extracellular matrices of these biofilms were composed mainly of carbohydrates and proteins involved in metabolic processes and the response to stimuli. We herein focus on one of these yeasts, S. etchellsii, to explore its molecular determinants for biofilm formation in depth. We describe new analyses in which the proteome of cells in the biofilm network formed in seawater-based media is compared to that of the planktonic cells co-existing with them and with cells suspended in freshwater-based growth media. According to our data, in both cases biofilms cells contain overexpressed proteins involved in protein biosynthesis, in membrane structures and in transport mediated by vesicles. The great number of proteins with higher expression in these cells participating in translation and located in ribosomes indicate that they are more engaged in protein biosynthesis than their counterparts. Analyses carried out with the STRING database reinforced these results. Cell viability was also wider in biofilm cells. Our analyses have also allowed us to detect in S. etchellsii a homolog of the Candida albicans Spf1p. This protein is an ion transporter P-type ATPase in this microorganism, which participates in several processes, including cellular adhesion and cell wall organization and biogenesis. Our work provides a dataset with a large number of unknown proteins of S. etchellsii that show sequence similarity to proteins from other yeasts; this knowledge will help to better understand the proteome of this yeast and to look for future biotechnological applications.

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耐盐壳斗霉菌在海水生长培养基中形成生物膜的蛋白质组学特征分析
微生物生物膜是细胞嵌入细胞外基质的复杂群落。我们之前发现,耐盐酵母菌 Debaryomyces fabryi、Schwanniomyces etchellsii、Schwanniomyces polymorphus 和 Kluyveromyces marxianus 在海水培养基中生长时能够形成生物膜,而在淡水培养基中则不能。这些生物膜的胞外基质主要由碳水化合物和蛋白质组成,参与新陈代谢过程并对刺激做出反应。在本文中,我们将重点关注其中一种酵母菌 S. etchellsii,深入探讨其形成生物膜的分子决定因素。我们描述了新的分析方法,将在海水培养基中形成的生物膜网络中的细胞的蛋白质组与与之共存的浮游细胞的蛋白质组以及悬浮在淡水培养基中的细胞的蛋白质组进行了比较。根据我们的数据,在这两种情况下,生物膜细胞都含有参与蛋白质生物合成、膜结构和囊泡运输的高表达蛋白质。在这些细胞中,大量参与翻译和位于核糖体中的蛋白质表达量较高,这表明它们比同类细胞更多地参与蛋白质的生物合成。利用 STRING 数据库进行的分析进一步证实了这些结果。生物膜细胞的存活率也更高。通过分析,我们还在 S. etchellsii 中发现了白色念珠菌 Spf1p 的同源物。这种蛋白质是该微生物的一种离子转运P型ATP酶,参与了多个过程,包括细胞粘附、细胞壁组织和生物生成。我们的工作提供了一个数据集,其中包含了大量与其他酵母蛋白质序列相似的 S. etchellsii 未知蛋白质;这些知识将有助于更好地了解这种酵母的蛋白质组,并寻找未来的生物技术应用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Current Research in Biotechnology
Current Research in Biotechnology Biochemistry, Genetics and Molecular Biology-Biotechnology
CiteScore
6.70
自引率
3.60%
发文量
50
审稿时长
38 days
期刊介绍: Current Research in Biotechnology (CRBIOT) is a new primary research, gold open access journal from Elsevier. CRBIOT publishes original papers, reviews, and short communications (including viewpoints and perspectives) resulting from research in biotechnology and biotech-associated disciplines. Current Research in Biotechnology is a peer-reviewed gold open access (OA) journal and upon acceptance all articles are permanently and freely available. It is a companion to the highly regarded review journal Current Opinion in Biotechnology (2018 CiteScore 8.450) and is part of the Current Opinion and Research (CO+RE) suite of journals. All CO+RE journals leverage the Current Opinion legacy-of editorial excellence, high-impact, and global reach-to ensure they are a widely read resource that is integral to scientists' workflow.
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