Current Research in Biotechnology最新文献

Gut health improvement by locally isolated probiotics and histomorphometric analysis in Wistar rats

IF 3.6 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Current Research in Biotechnology

Pub Date : 2025-01-01 DOI: 10.1016/j.crbiot.2024.100271

Zuhra Bibi , Dilara Abbas Bukhari , Muhammad Qadeer Sarwar , Arifullah , Samina Younas , Tayyab Manzoor , Abdul Rehman

In the present investigation, lab-isolated probiotics Weisella confusa MZ735961.1, Lactiplantibacillus plantarum MZ707748.1, L. plantarum MZ710117.1, and L. plantarum MZ735961 were used separately and in combinations to evaluate their effect on gut morphology of Wistar rats. Synergistic groups were formed by 1:1 and labeled as G1 (L. plantarum MZ707748.1 and L. plantarum MZ729681.1), G2 (W. confusa MZ735961.1 and L. plantarum MZ727611.1), G3 (L. plantarum MZ729681.1, W. confusa MZ735961.1, and Lactobacillus acidophilus La-14), G4 (all above mentioned probiotics). Rats were gavage-fed with probiotics according to their colony-forming unit (CFU). The experiment was carried out for 35 days. The bacteria were re-isolated from the gut and identified by biochemical tests which confirmed the administration and re-isolation of different Lactobacillus strains from the gut. Molecular characterization was done through 16S rRNA by using universal primers. After sequencing eight Lactobacillus strains were identified. Histopathology of rats’ intestines was done, and different parameters were examined. Villus height, crypt height, crypt width, mucosa, and sub-mucosa of jejunum were significantly (p = 0.00) increased in the G3 synergetic probiotic group compared to 0-day and negative control. However, the villus width showed non-significant (p > 0.05) variations in both genders. Mucosa tunic, muscle tunic, total wall, and crypt depth were significantly increased (p = 0.00) in the G4 group of medial colon. The study concluded that gut morphology improves as probiotics adhere better to the intestinal epithelium, excluding pathogens, reducing inflammation, enhancing nutrient absorption, and stimulating mucosal growth. This results in improved villus structure and gut wall integrity.

{"title":"Gut health improvement by locally isolated probiotics and histomorphometric analysis in Wistar rats","authors":"Zuhra Bibi , Dilara Abbas Bukhari , Muhammad Qadeer Sarwar , Arifullah , Samina Younas , Tayyab Manzoor , Abdul Rehman","doi":"10.1016/j.crbiot.2024.100271","DOIUrl":"10.1016/j.crbiot.2024.100271","url":null,"abstract":"<div><div>In the present investigation, lab-isolated probiotics <em>Weisella confusa</em> MZ735961.1, <em>Lactiplantibacillus plantarum</em> MZ707748.1<em>, L. plantarum</em> MZ710117.1<em>,</em> and <em>L. plantarum</em> MZ735961 were used separately and in combinations to evaluate their effect on gut morphology of Wistar rats. Synergistic groups were formed by 1:1 and labeled as G1 (<em>L. plantarum</em> MZ707748.1 and <em>L. plantarum</em> MZ729681.1), G2 (<em>W. confusa</em> MZ735961.1 and <em>L. plantarum</em> MZ727611.1), G3 (<em>L. plantarum</em> MZ729681.1, <em>W. confusa</em> MZ735961.1, and <em>Lactobacillus acidophilus</em> La-14), G4 (all above mentioned probiotics). Rats were gavage-fed with probiotics according to their colony-forming unit (CFU). The experiment was carried out for 35 days. The bacteria were re-isolated from the gut and identified by biochemical tests which confirmed the administration and re-isolation of different <em>Lactobacillus</em> strains from the gut. Molecular characterization was done through 16S rRNA by using universal primers. After sequencing eight <em>Lactobacillus</em> strains were identified. Histopathology of rats’ intestines was done, and different parameters were examined. Villus height, crypt height, crypt width, mucosa, and sub-mucosa of jejunum were significantly (p = 0.00) increased in the G3 synergetic probiotic group compared to 0-day and negative control. However, the villus width showed non-significant (p > 0.05) variations in both genders. Mucosa tunic, muscle tunic, total wall, and crypt depth were significantly increased (p = 0.00) in the G4 group of medial colon. The study concluded that gut morphology improves as probiotics adhere better to the intestinal epithelium, excluding pathogens, reducing inflammation, enhancing nutrient absorption, and stimulating mucosal growth. This results in improved villus structure and gut wall integrity.</div></div>","PeriodicalId":52676,"journal":{"name":"Current Research in Biotechnology","volume":"9 ","pages":"Article 100271"},"PeriodicalIF":3.6,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143136459","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Curcumol inhibits hepatocellular carcinoma proliferation through miRNA-124/STAT3 pathway: Network pharmacology and experimental validation

IF 3.6 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Current Research in Biotechnology

Pub Date : 2025-01-01 DOI: 10.1016/j.crbiot.2024.100270

Gui-yu Li , Ji-yong Lin

Objective

Hepatocellular carcinoma (HCC) is the most common type of liver cancer and is one of the most common global cancers. Curcuma zedoaria (Christm.) Roscoe is a traditional Chinese herb that has been used for thousands of years in China to treat various types of cancer. Curcumol is one of its primary bioactive sesquiterpenes and has been reported to possess antitumor properties; however, the underlying mechanisms in hepatocellular carcinoma (HCC) are largely unknown. The aim of this study was to reveal the mechanism of curcumol treating HCC based on the network pharmacology and experimental verification.

Materials and Methods

Targets of HCC and curcumol were identified. The drugs and disease targets were intersected by Venn Diagram. The KEGG pathway enrichment analysis of curcumol treating HCC was analyzed through the R 3.6.1 software. The effects of curcumol on the inhibition of HCC cell line HepG2 growth and its pro-apoptotic activity were evaluated by cell counting kit-8 and flow cytometry. The expression of microRNA-124 (miRNA-124) mRNA was detected by quantitative real-time PCR. HepG2 cells were transfected with a miRNA mimic and inhibitor. The expression of STAT3 and its phosphorylation were induced by IL-6 and detected by western blotting.

Results

MicroRNAs in cancer is a significant enrichment signaling pathway for curcumol treating HCC, according to the KEGG pathway analysis. Curcumol effectively inhibited HepG2 cell growth at 50–150 μg/ml, while it had low toxicity to normal LO2 cells. Using flow cytometry, curcumol strongly promoted apoptosis in HepG2 cells and was more potent than the miRNA-124 mimic, whereas the miRNA-124 inhibitor reduced the pro-apoptotic effect of curcumol. Western blotting revealed that curcumol significantly downregulated the overexpression of STAT3 and its phosphorylation in interleukin-6 induced HepG2 cells, whereas an increased level of STAT3 was observed in the miRNA-124 inhibitor transfected cells after curcumol treatment compared to untransfected cells. The level of miRNA-124 was changed up to 5.87-fold by curcumol treatment.

Conclusions

The mechanism underlying the effect of curcumol on inhibition and pro-apoptosis of HepG2 cell growth is possibly related to the miRNA-124/STAT3 pathway.

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引用次数: 0

Termitomyces mushroom extract-mediated synthesis of silver nanoparticles and its in-vitro activity against drug-resistant Candida species

IF 3.6 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Current Research in Biotechnology

Pub Date : 2025-01-01 DOI: 10.1016/j.crbiot.2025.100279

Naheem Adekilekun Tijani , Joseph Hokello , Emmanuel Eilu , Saheed Adekunle Akinola , Abdullateef Opeyemi Afolabi , Ibrahim Ntulume , Ismail Abiola Adebayo

Green nanotechnology has continued to gain popularity as a novel and alternative strategy to overcome the menace caused by drug-resistant pathogens. For the first time, this study explores an efficient, eco-friendly, and economical approach for the mycogenic synthesis of silver nanoparticles (AgNPs) by utilizing the aqueous extract of wild Termitomyces species of edible mushroom. The mushroom-assisted AgNPs synthesis was validated with visual colour observation and characterized with UV–Vis spectrophotometer, SEM, EDX, XRD, FTIR and DLS. The potential anticandidal efficacy of the synthesized AgNPs was investigated against six clinical isolates of resistant pathogenic Candida species. A typical Ag surface plasmon resonance (SPR) had absorbance maxima wavelength within 371–404 nm range, with a spherical shape particulate structure in the size range of 28 to 45 nm according to UV–Vis and SEM analyses respectively. Remarkable antifungal activity was recorded against a good number of the Candida isolates with MICs values in the range of 0.0122–0.0976 mg/mL. We conclude that wild Termitomyces mushroom is a suitable biomaterial for AgNPs synthesis and an effective antifungal agent which could be adopted as a novel therapeutic agent for efficient management of drug-resistant Candida pathogens.

{"title":"Termitomyces mushroom extract-mediated synthesis of silver nanoparticles and its in-vitro activity against drug-resistant Candida species","authors":"Naheem Adekilekun Tijani , Joseph Hokello , Emmanuel Eilu , Saheed Adekunle Akinola , Abdullateef Opeyemi Afolabi , Ibrahim Ntulume , Ismail Abiola Adebayo","doi":"10.1016/j.crbiot.2025.100279","DOIUrl":"10.1016/j.crbiot.2025.100279","url":null,"abstract":"<div><div>Green nanotechnology has continued to gain popularity as a novel and alternative strategy to overcome the menace caused by drug-resistant pathogens. For the first time, this study explores an efficient, eco-friendly, and economical approach for the mycogenic synthesis of silver nanoparticles (AgNPs) by utilizing the aqueous extract of wild <em>Termitomyces</em> species of edible mushroom. The mushroom-assisted AgNPs synthesis was validated with visual colour observation and characterized with UV–Vis spectrophotometer, SEM, EDX, XRD, FTIR and DLS. The potential anticandidal efficacy of the synthesized AgNPs was investigated against six clinical isolates of resistant pathogenic <em>Candida</em> species. A typical Ag surface plasmon resonance (SPR) had absorbance maxima wavelength within 371–404 nm range, with a spherical shape particulate structure in the size range of 28 to 45 nm according to UV–Vis and SEM analyses respectively. Remarkable antifungal activity was recorded against a good number of the <em>Candida</em> isolates with MICs values in the range of 0.0122–0.0976 mg/mL. We conclude that wild <em>Termitomyces</em> mushroom is a suitable biomaterial for AgNPs synthesis and an effective antifungal agent which could be adopted as a novel therapeutic agent for efficient management of drug-resistant <em>Candida</em> pathogens.</div></div>","PeriodicalId":52676,"journal":{"name":"Current Research in Biotechnology","volume":"9 ","pages":"Article 100279"},"PeriodicalIF":3.6,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143429012","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ranking economic and environmental performance of feedstocks used in bio-based production systems

IF 3.6 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Current Research in Biotechnology

Pub Date : 2025-01-01 DOI: 10.1016/j.crbiot.2025.100275

Dania Muhieddine Orfali , Samir Meramo , Sumesh Sukumara

Biotechnology offers renewable alternatives for producing food, materials, and numerous functional compounds. While rampant human activities are disrupting planets’ geophysical flows, it is urgent to develop sustainable solutions with novel feedstocks and innovative valorization pathways. With the need to reduce greenhouse gas emissions and enhance circularity, new raw materials termed the next-generation feedstocks (NGFs), such as carbon dioxide, methane, methanol, formic acid, and acetic acid, have emerged as potential feedstocks for bio-based processes. So far, no such review exists that compares the performance of conventional, sugar, lignocellulosic, algae-based feedstocks, and NGFs, which biotechnology could upcycle into a wide range of products. In this review, the economic and environmental performances of the feedstocks are analyzed, and quantifications are presented and standardized based on techno-economic analysis and life cycle assessment models. The main parameters for comparison included the geographical location, unit production cost, and environmental impact categories. The results show that the economic and environmental performances are highly variable among the different feedstocks and their processing routes, also depicting evident tradeoffs. Carbon dioxide, sugar cane molasses and glycerol from waste streams are performing better on assessed indicators overall than other potential feedstocks. Nonetheless, this designed data source is the first step for reliable feedstock selection based on sustainability criteria.

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引用次数: 0

Design of a thermal stress microfluidic platform to screen stability of therapeutic proteins in pharmaceutical formulations

IF 3.6 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Current Research in Biotechnology

Pub Date : 2025-01-01 DOI: 10.1016/j.crbiot.2025.100273

O. Bortone , S. Fiorenza , M. Baldassarre , N. Falco , M. Amidi , T. Markkula , P.A. Netti , E. Torino

Therapeutic proteins have great potentialities for the care of a wide spectrum of diseases, for which other small synthetic drugs result ineffective. Due to challenges related to their immunogenicity, the journey of biologics into clinics still faces obstacles. Among the causes of protein immunogenicity, their natural propensity to aggregation is crucial, indeed, to study their stability, pharmaceutical formulations are generally exposed to diverse environmental physicochemical conditions. Traditional approaches to explore protein behavior are effort-demanding, lengthy and expensive, resulting in a limited knowledge of biomolecule stability. There is an urgent need to develop faster and more cost-effective technologies for biological formulation development. In this work, the conceptualization, design and implementation of a modular and automated microfluidic platform to provide thermal stress to highly concentrated and viscous pharmaceutical formulations is presented. The microfluidic platform validity in terms of reliability and comparability to a forced degradation batch-wise stimulation is demonstrated by thermally stimulating and analyzing through SE-HPLC (Size Exclusion – High Performance Liquid Chromatography) different high concentration (> 100 mg/ml) therapeutic nanobody-based formulations. Remarkably, the ranking of the formulations returned by the microfluidic thermal stress platform follows the same trend obtained through well-established industrial in-batch stimulations. Furthermore, data coming from microfluidic stimulations well correlates to outcomes coming from industrial methodologies for storage and accelerated stability studies.

{"title":"Design of a thermal stress microfluidic platform to screen stability of therapeutic proteins in pharmaceutical formulations","authors":"O. Bortone , S. Fiorenza , M. Baldassarre , N. Falco , M. Amidi , T. Markkula , P.A. Netti , E. Torino","doi":"10.1016/j.crbiot.2025.100273","DOIUrl":"10.1016/j.crbiot.2025.100273","url":null,"abstract":"<div><div>Therapeutic proteins have great potentialities for the care of a wide spectrum of diseases, for which other small synthetic drugs result ineffective. Due to challenges related to their immunogenicity, the journey of biologics into clinics still faces obstacles. Among the causes of protein immunogenicity, their natural propensity to aggregation is crucial, indeed, to study their stability, pharmaceutical formulations are generally exposed to diverse environmental physicochemical conditions. Traditional approaches to explore protein behavior are effort-demanding, lengthy and expensive, resulting in a limited knowledge of biomolecule stability. There is an urgent need to develop faster and more cost-effective technologies for biological formulation development. In this work, the conceptualization, design and implementation of a modular and automated microfluidic platform to provide thermal stress to highly concentrated and viscous pharmaceutical formulations is presented. The microfluidic platform validity in terms of reliability and comparability to a forced degradation batch-wise stimulation is demonstrated by thermally stimulating and analyzing through SE-HPLC (Size Exclusion – High Performance Liquid Chromatography) different high concentration (> 100 mg/ml) therapeutic nanobody-based formulations. Remarkably, the ranking of the formulations returned by the microfluidic thermal stress platform follows the same trend obtained through well-established industrial in-batch stimulations. Furthermore, data coming from microfluidic stimulations well correlates to outcomes coming from industrial methodologies for storage and accelerated stability studies.</div></div>","PeriodicalId":52676,"journal":{"name":"Current Research in Biotechnology","volume":"9 ","pages":"Article 100273"},"PeriodicalIF":3.6,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143136460","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Importance of substrate type and its constituents on overall performance of microbial fuel cells

IF 3.6 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Current Research in Biotechnology

Pub Date : 2025-01-01 DOI: 10.1016/j.crbiot.2025.100272

Somil Thakur, Rajnish Kaur Calay, Mohamad Y. Mustafa, Fasil E. Eregno, Ravindra R. Patil

Microbial Fuel Cells (MFC) have emerged as a potential wastewater treatment technology that utilizes metabolic processes of microorganisms present in the wastewater to disintegrate organic substrates and harness direct electricity. This paper reviews the potential of different wastewater types as a suitable substrate for microbial activities in MFCs. Substrate composition (carbon source, nutrient content and inhibitory compounds) directly affects the microbial growth, wastewater treatment potential, electron transfer rate and power harvested. Readily biodegradable substrates such as acetate and glucose promote microbial metabolism and electron transport, thus resulting in enhanced power generation. Substrates such as municipal or agricultural wastewater that constitute both simple and complex organic matter require longer breakdown durations but can provide MFCs with long term operational stability. On the other hand, substrates such as leachate from landfills, mining wastewater etc. are generally unsuitable for regular operations. The paper discusses the challenges such as suitability of various wastewaters, unpredictability of composition, presence of inhibitory chemicals like heavy metals or toxic organics that can subdue the microbial activity and reduce efficiency of a MFC reactor. It aims to identify the relationship between the substrate characteristics and MFC performance in order to select the substrate for achieving optimal output from MFC technology. The suitable substrates that exhibit optimal performance in terms of current and power output, coulombic efficiency, and reduction in chemical oxygen demand are identified.

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引用次数: 0

Biogenic silver nanoparticles produced by Trichoderma reesei inhibit SARS-CoV-2 infection, reduce lung viral load and ameliorate acute pulmonary inflammation

IF 3.6 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Current Research in Biotechnology

Pub Date : 2025-01-01 DOI: 10.1016/j.crbiot.2025.100277

Marcus V.M.V. Amaral , Cláudia B. Carraro , Amanda C.C. Antoniêto , Mariana N. Costa , Thais F.C. Fraga-Silva , Ualter G. Cipriano , Rodrigo P.F. Abuná , Tamara S. Rodrigues , Ronaldo B. Martins , Andreia M. Luzenti , Glaucia R. Caruso , Priscyla D. Marcato , Vania L.D. Bonato , Dario S. Zamboni , Bergman M. Ribeiro , Sônia N. Báo , Joao S. da Silva , Flávio P. Veras , Roberto N. Silva

The COVID-19 pandemic, caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), posed a significant global health challenge and still demands efforts to develop new therapies. In this study, we investigated the potential of biogenic silver nanoparticles (AgNPs) synthesized by the fungus Trichoderma reesei to combat SARS-CoV-2 infection. In silico studies showed that AgNPs, ranging from 7 nm to 50 nm, have high affinity for spike protein from different variant of SARS-CoV-2. Our findings show that AgNPs effectively do not affect cell viability in Calu-3 cells, inhibit viral infection in Vero-E6 cells and progression of infection in vitro. Additionally, AgNPs impair caspase-1 activation, lactate dehydrogenase release and IL-1β production by human monocytes. Moreover, our study reveals that AgNPs treatment significantly alleviated acute lung injury induced by SARS-CoV-2 infection in Syrian hamsters. This suggests that AgNPs treatment effectively impairs viral replication or propagation within lung tissue, highlighting its potential as an antiviral agent against SARS-CoV-2. Further investigations are warranted to elucidate the underlying mechanisms of action of AgNPs and to assess their safety and efficacy in clinical settings. Nonetheless, our findings offer promising insights into the development of novel therapeutic strategies for combating COVID-19 and reducing its associated morbidity and mortality.

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引用次数: 0

Curcumin dietary supplementation enhances serum carbohydrate-degrading enzyme activities in buffaloes

IF 3.6 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Current Research in Biotechnology

Pub Date : 2025-01-01 DOI: 10.1016/j.crbiot.2025.100276

Maima Matin , Natalia Ksepka , Kamil Wysocki , Michel-Edwar Mickael , Alpana Pande , Jarosław Olav Horbańczuk , Artur Jóźwik , Olga Adamska , Artur Stolarczyk , Laszlo Barna Iantovics , Yordanka Ilieva , Pencho Penchev , Atanas G. Atanasov

Curcumin, the major bioactive compound found in turmeric (Curcuma longa) is a subject of intensive research, due to its multiple bioactivities. Previous studies have investigated metabolic effects of curcumin in humans or established animal research models such as mice, but studies with farm animals have been scarce. In this work, we aimed to study the effects of curcumin supplementation in Bulgarian Murrah buffaloes (Bubalus bubalis), on the serum activities of seven important carbohydrate-metabolizing enzymes, β-glucuronidase, α-galactosidase, α-glucosidase, β-glucosidase, β-galactosidase, N-acetyl-hexosaminidase, and mannosidase. Curcumin was supplemented at a dose of 50 g per animal, per day, and serum activities of the studied enzymes were determined at the start (day 0), middle (day 14), and end (day 28) of the supplementation period. A tendency for increased activity of all studied enzymes was observed upon supplementation with curcumin, with the activity levels of five of the enzymes displaying statistically significant upregulation at day 28 (β-glucuronidase, α-galactosidase, β-galactosidase, N-acetyl-hexosaminidase, and mannosidase). These upregulated activities are in general indicative of increased carbohydrates turnover and detoxification processes, and might provide clues for a better understanding of the mechanisms of action of curcumin in vivo, as well as for novel approaches to rationally impact animal or human health and wellbeing through targeted supplementation.

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引用次数: 0

A systematic review on Indian Acacia species

IF 3.6 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Current Research in Biotechnology

Pub Date : 2025-01-01 DOI: 10.1016/j.crbiot.2025.100274

Bharat Singh , Jagdish Prasad , Ram Avtar Sharma

Acacia plants are evergreen, perennial, short, flat-crowned to long and straight-bole trees. A total of 34 species of Acacia genus are distributed in different states of India. Acacia plants are used in the treatment of chronic dysentery, diarrhoea, throat troubles, conjunctivitis and stomatitis. The critical data of ethnopharmacological properties, phytochemical and pharmacological attributes of 27 species were obtained through extensive review of relevant reference books, M. Sc./M. Tech dissertations, PhD theses, Web of Science, PubMed, Google Scholar, ScienceDirect, Wiley, Taylors & Francis, Bentham, Thieme, Springer, and eFloras. The flavone glycosides, flavonoids, lignans, anthraquinones, monoterpenes, and alkaloids have been identified from 20 species of Indian Acacia species. Indian Acacia species possess antimicrobial, antidiabetic, antiarthritic, antistress, anti-inflammatory, antioxidant, anticancer, and wound healing properties. However, various reports are available in literature on phytochemistry and biological properties of 27 Indian Acacia species but, still 7 species have not been evaluated for their pharmacological studies. The review discusses updated knowledge of ethnopharmacological applications, phytochemical and pharmacological attributes, and pharmacokinetic profiles of Indian Acacia species.

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引用次数: 0

Life cycle and environmental impact assessment of vegetation-activated sludge process (V-ASP) for decentralized wastewater treatment 用于分散式污水处理的植被活性污泥法（V-ASP）的生命周期和环境影响评估

IF 5.6 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Current Research in Biotechnology

Pub Date : 2024-01-01 DOI: 10.1016/j.crbiot.2023.100172

Pu Li , Xiaomeng Hu , Jiajia Yuan , Feiyun Sun , Pengfei Li , Wenyi Dong , Erdeng Du , Mingguo Peng

The integrated Vegetation-Activated Sludge Process (V-ASP) has emerged as a promising solution for decentralized wastewater treatment, offering a unique combination of landscape vegetation with the traditional Anaerobic-Anoxic-Oxic process (AAO). The experiments displayed that V-ASP consistently demonstrated remarkable treatment performance, maintaining stable removal efficiency exceeding 90 % for COD, NH₄⁺-N, and TP. The vegetation growth is helpful for the removal of pollutants and the microbial community in the allocated vegetation root, while the bulk suspended sludge was changed significantly by using PCR test. To comprehensively evaluate the environmental footprint of V-ASP, a Life Cycle Assessment (LCA) was conducted. The mass balance calculation containing wastewater treatment performance, energy consumption, vegetation growth behavior, greenhouse gas (GHG) emissions, treated water, and sludge discharge was carried out to establish the life cycle inventories (LCI). SimaPro 9.0 software and the ReCiPe (H) midpoint impact assessment method were employed, which revealed that the V-ASP system boasts low GHG emissions and freshwater eutrophication potential compared to the traditional AAO process. In essence, this study provides a comprehensive understanding of the V-ASP system, especially the environmental impacts, guiding its potential for sustainable decentralized sewage treatment applications.

综合植被-活性污泥法（V-ASP）将景观植被与传统的厌氧-缺氧-缺氧工艺（AAO）独特地结合在一起，成为一种前景广阔的分散式污水处理解决方案。实验表明，V-ASP 的处理效果显著，对 COD、NH4+-N 和 TP 的去除率稳定在 90% 以上。植被的生长有助于污染物的去除和分配植被根部的微生物群落，而通过 PCR 测试，大量悬浮污泥发生了显著变化。为全面评估 V-ASP 的环境足迹，进行了生命周期评估（LCA）。质量平衡计算包括废水处理性能、能耗、植被生长行为、温室气体排放、处理水和污泥排放，以建立生命周期清单（LCI）。采用 SimaPro 9.0 软件和 ReCiPe (H) 中点影响评估方法，结果表明与传统 AAO 工艺相比，V-ASP 系统具有较低的温室气体排放和淡水富营养化潜力。从本质上讲，本研究提供了对 V-ASP 系统的全面了解，尤其是对环境影响的了解，并为其在可持续分散式污水处理应用中的潜力提供了指导。

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引用次数: 0