Promising anti-inflammatory activity of a novel designed anti-microbial peptide for wound healing

IF 3.2 3区医学 Q2 CRITICAL CARE MEDICINE Burns Pub Date : 2024-07-31 DOI:10.1016/j.burns.2024.07.036

Fariba Fathi, Maryam Ghobeh, Farshad H. Shirazi, Maryam Tabarzad

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Abstract

Chronic wounds can develop as a result of prolonged inflammation during the healing process, which can happen due to bacterial infection. Therefore, preventing infection and controlling inflammation can accelerate wound healing. Antimicrobial peptides have different protective properties in addition to antimicrobial activity. Some of these activities include the stimulation of cytokine or chemokine synthesis, the facilitation of chemotaxis and cell proliferation, the acceleration of cell proliferation, the induction of anti-inflammatory responses, and the promotion of wound repair. This study aimed to assess the wound healing potential of a novel in silico-designed antimicrobial peptide. Then, its anti-inflammatory activity was investigated by measuring the level of tumor necrosis factor-α (TNF-α) and transforming growth factor beta (TGF-β) as indicators of the wound healing process. In addition, the influence of the peptide on cell migration was evaluated by a scratch test on human dermal fibroblasts (HDF) and HaCaT cells as a human epidermal keratinocyte cell line. The results showed that our new peptide could act well in inhibiting TNF-α over-secretion while increasing the expression of TGF-β as an anti-inflammatory factor. This peptide showed a significant potential to stimulate HDF and HaCaT cell migration and proliferation. Therefore, using this peptide as an anti-inflammatory component of wound dressings may be promising.

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用于伤口愈合的新型抗微生物肽具有良好的抗炎活性

在伤口愈合过程中，细菌感染可能导致伤口长期发炎，从而形成慢性伤口。因此，预防感染和控制炎症可以加速伤口愈合。抗菌肽除了具有抗菌活性外，还具有不同的保护特性。其中一些活性包括刺激细胞因子或趋化因子的合成、促进趋化和细胞增殖、加速细胞增殖、诱导抗炎反应以及促进伤口修复。本研究旨在评估一种新型硅设计抗菌肽的伤口愈合潜力。首先，通过测量肿瘤坏死因子-α（TNF-α）和转化生长因子β（TGF-β）的水平来评估该抗菌肽的伤口愈合潜力；然后，通过测量作为伤口愈合过程指标的肿瘤坏死因子-α（TNF-α）和转化生长因子β（TGF-β）的水平来研究该抗菌肽的抗炎活性。此外，还通过对人真皮成纤维细胞（HDF）和人表皮角质细胞系 HaCaT 细胞进行划痕试验，评估了多肽对细胞迁移的影响。结果表明，我们的新肽能很好地抑制 TNF-α 的过度分泌，同时增加作为抗炎因子的 TGF-β 的表达。该肽具有刺激 HDF 和 HaCaT 细胞迁移和增殖的显著潜力。因此，使用这种肽作为伤口敷料的抗炎成分可能很有前景。

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来源期刊

Burns 医学-皮肤病学

CiteScore

4.50

自引率

18.50%

发文量

304

审稿时长

72 days

期刊介绍： Burns aims to foster the exchange of information among all engaged in preventing and treating the effects of burns. The journal focuses on clinical, scientific and social aspects of these injuries and covers the prevention of the injury, the epidemiology of such injuries and all aspects of treatment including development of new techniques and technologies and verification of existing ones. Regular features include clinical and scientific papers, state of the art reviews and descriptions of burn-care in practice. Topics covered by Burns include: the effects of smoke on man and animals, their tissues and cells; the responses to and treatment of patients and animals with chemical injuries to the skin; the biological and clinical effects of cold injuries; surgical techniques which are, or may be relevant to the treatment of burned patients during the acute or reconstructive phase following injury; well controlled laboratory studies of the effectiveness of anti-microbial agents on infection and new materials on scarring and healing; inflammatory responses to injury, effectiveness of related agents and other compounds used to modify the physiological and cellular responses to the injury; experimental studies of burns and the outcome of burn wound healing; regenerative medicine concerning the skin.