The ameliorative effect of Naringenin on fenamiphos induced hepatotoxicity and nephrotoxicity in a rat model: Oxidative stress, inflammatory markers, biochemical, histopathological, immunohistochemical and electron microscopy study

IF 3.9 3区医学 Q2 FOOD SCIENCE & TECHNOLOGY Food and Chemical Toxicology Pub Date : 2024-08-10 DOI:10.1016/j.fct.2024.114911

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Abstract

Fenamiphos (FNP) is an organophospate pesticide that causes many potential toxicities in non-target organisms. Naringenin (NAR) has protective properties against oxidative stress. In this study, FNP (0.76 mg/kg bw) toxicity and the effect of NAR (50 mg/kg bw) on the liver and kidney of rats were investigated via biochemical, oxidative stress, immunohistochemical, cytopathological and histopathologically. As a result of biochemical studies, FNP caused oxidative stress in tissues with a change in total antioxidant/oxidant status. After treatment with FNP, hepatic and renal levels of AChE were significantly reduced while 8-OHdG and IL-17 levels, caspase-3 and TNF-α immunoreactivity increased compared to the control group. It also changed in serum biochemical markers such as ALT, AST, BUN, creatinine. Exposure to FNP significantly induced cytopathological, histopathological and immunohistochemical changes through tissue damage. NAR treatment restored biochemical parameters, renal/hepatic AChE, ultrastructural, histopathological and immunohistochemical changes modulated and blocked the increasing effect of FNP on tissues caspase-3 and TNF-α expressions, 8-OHdG and IL-17 levels. In electron microscopy studies, swelling was observed in the mitochondria of the cells in both tissues of the FNP-treated rats, while less ultrastructural changes in the FNP plus NAR-treated rats.

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柚皮苷对甲胺磷诱导的大鼠肝毒性和肾毒性的改善作用：氧化应激、炎症指标、生化、组织病理学、免疫组织化学和电子显微镜研究。

苯线磷 (FNP) 是一种有机磷农药，对非目标生物有许多潜在毒性。柚皮苷（NAR）对氧化应激具有保护作用。本研究通过生化、氧化应激、免疫组织化学、细胞病理学和组织病理学等方法研究了 FNP（0.76 毫克/千克体重）对大鼠肝脏和肾脏的毒性以及 NAR（50 毫克/千克体重）对大鼠肝脏和肾脏的影响。生化研究结果表明，FNP 会导致组织氧化应激，并改变总抗氧化剂/氧化剂状态。与对照组相比，使用 FNP 治疗后，肝脏和肾脏的 AChE 水平明显降低，而 8-OHdG 和 IL-17 水平、caspase-3 和 TNF-α 免疫活性则有所升高。血清生化指标如谷丙转氨酶（ALT）、谷草转氨酶（AST）、尿素氮（BUN）和肌酐（Creatinine）也发生了变化。暴露于 FNP 会通过组织损伤明显诱导细胞病理学、组织病理学和免疫组化变化。NAR 治疗可恢复生化参数、肾/肝 AChE、超微结构、组织病理学和免疫组化变化，并阻断 FNP 对组织中 caspase-3 和 TNF-α 表达、8-OHdG 和 IL-17 水平的增加效应。在电子显微镜研究中，观察到 FNP 处理的大鼠两种组织的细胞线粒体都出现肿胀，而 FNP 加 NAR 处理的大鼠超微结构变化较小。

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来源期刊

Food and Chemical Toxicology 工程技术-毒理学

CiteScore

10.90

自引率

4.70%

发文量

651

审稿时长

31 days

期刊介绍： Food and Chemical Toxicology (FCT), an internationally renowned journal, that publishes original research articles and reviews on toxic effects, in animals and humans, of natural or synthetic chemicals occurring in the human environment with particular emphasis on food, drugs, and chemicals, including agricultural and industrial safety, and consumer product safety. Areas such as safety evaluation of novel foods and ingredients, biotechnologically-derived products, and nanomaterials are included in the scope of the journal. FCT also encourages submission of papers on inter-relationships between nutrition and toxicology and on in vitro techniques, particularly those fostering the 3 Rs. The principal aim of the journal is to publish high impact, scholarly work and to serve as a multidisciplinary forum for research in toxicology. Papers submitted will be judged on the basis of scientific originality and contribution to the field, quality and subject matter. Studies should address at least one of the following: -Adverse physiological/biochemical, or pathological changes induced by specific defined substances -New techniques for assessing potential toxicity, including molecular biology -Mechanisms underlying toxic phenomena -Toxicological examinations of specific chemicals or consumer products, both those showing adverse effects and those demonstrating safety, that meet current standards of scientific acceptability. Authors must clearly and briefly identify what novel toxic effect (s) or toxic mechanism (s) of the chemical are being reported and what their significance is in the abstract. Furthermore, sufficient doses should be included in order to provide information on NOAEL/LOAEL values.