Comprehensive spatial distribution profiling of bioactive emodin in mouse organs using mass spectrometry imaging

IF 1.8 4区化学 Q3 CHEMISTRY, ANALYTICAL Analytical Sciences Pub Date : 2024-08-13 DOI:10.1007/s44211-024-00644-1

Si-Qi Han, Xiao-Kang Fu, Wei Ha, Yan-Ping Shi

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Abstract

Emodin is an important anthraquinone compound with good anti-inflammatory activity in Chinese traditional medicine rhubarb. Detailed spatial distribution information in bio-tissues plays an important role in revealing the pharmacodynamics, toxicology and chemical mechanism of emodin. Herein, the matrix-assisted laser desorption/ionization time-of-flight mass spectrometry imaging (MALDI-TOF-MSI) analytical method was established to obtain information on the spatial and temporal changes of emodin in multiple mouse tissue sections (heart, liver, spleen, lung, kidney, and brain) after intraperitoneal injection of emodin in mice. The measurements were accomplished in the negative ion mode in the range of m/z 250–285 Da with a spatial resolution on 40 µm. It was found that emodin was predominantly distributed in the arteriolar vascular region of the heart, the capsule region of the spleen, and the cortex of the kidney. Moreover, the MALDI-TOF-MSI result implied that emodin might be distributed in the brain. These more detailed spatial distribution information provides the significant reference for investigating the action mechanism of emodin, which cannot be obtained from conventional LC–MS analysis. The distribution trend of emodin in the results of MALDI-TOF-MSI analysis agreed with the ultra-performance liquid chromatography/tandem mass spectrometry (UPLC-MS/MS) results well, demonstrating the complementarity and reliability of the established MALDI-TOF-MSI method. Our work provided a label-free molecular imaging method to investigate the precise spatial distribution of emodin in various organs, which prove great potential in studying the effective substances and mechanism of rhubarb.

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利用质谱成像技术全面分析生物活性大黄素在小鼠器官中的空间分布。

大黄素是中药大黄中一种重要的蒽醌类化合物，具有良好的抗炎活性。大黄素在生物组织中的详细空间分布信息对揭示其药效学、毒理学和化学机制具有重要作用。本文建立了基质辅助激光解吸电离飞行时间质谱成像（MALDI-TOF-MSI）分析方法，以获得小鼠腹腔注射大黄素后，大黄素在多个小鼠组织切片（心、肝、脾、肺、肾和脑）中的时空变化信息。测量是在 m/z 250-285 Da 的负离子模式下进行的，空间分辨率为 40 µm。结果发现，大黄素主要分布在心脏动脉血管区、脾脏囊区和肾脏皮质。此外，MALDI-TOF-MSI 的结果还暗示大黄素可能分布在大脑中。这些更详细的空间分布信息为研究大黄素的作用机制提供了重要参考，而这些信息是传统的LC-MS分析无法获得的。MALDI-TOF-MSI分析结果中大黄素的分布趋势与超高效液相色谱/串联质谱（UPLC-MS/MS）分析结果高度吻合，证明了MALDI-TOF-MSI方法的互补性和可靠性。我们的工作为研究大黄素在各器官中的精确空间分布提供了一种无标记的分子成像方法，这证明它在研究大黄的有效物质和机制方面具有很大的潜力。

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来源期刊

Analytical Sciences 化学-分析化学

CiteScore

2.90

自引率

18.80%

发文量

232

审稿时长

1 months

期刊介绍： Analytical Sciences is an international journal published monthly by The Japan Society for Analytical Chemistry. The journal publishes papers on all aspects of the theory and practice of analytical sciences, including fundamental and applied, inorganic and organic, wet chemical and instrumental methods. This publication is supported in part by the Grant-in-Aid for Publication of Scientific Research Result of the Japanese Ministry of Education, Culture, Sports, Science and Technology.