Analytical Sciences最新文献

英文中文

Aggregation-induced emission luminogens for volatile organic compound detection. 用于挥发性有机化合物检测的聚集诱导发射发光光源。

IF 2 4区化学 Q3 CHEMISTRY, ANALYTICAL

Analytical Sciences

Pub Date : 2025-12-10 DOI: 10.1007/s44211-025-00856-z

Muneeba Amin, Iqra Gulzar, Qurashi Najmu Saqib, Muzaffer Ahmad, Adil Majeed Rather, Nusrat Shafi, Waseem A Wani, Jahangir Ahmad Rather, Waheed Ahmad Khanday, Abdul Haleem Wani, Akhtar Hussain Malik, Faiza Habib

Volatile organic compounds (VOCs) are crucial components in many daily-use products, yet they pose a significant threat to public health and the environment, even in minute amounts, due to their high toxicity. Therefore, it is crucial to develop a reliable method that could track these hazardous vapours with high sensitivity and selectivity. Among various techniques, fluorescence-based detection strategies have demonstrated great efficiency in both qualitative and quantitative monitoring of VOCs in real-time. These methods are attractive due to their low cost and portability, making them suitable for on-site applications. This review explores the latest advancements in aggregation-induced emission (AIE)-based fluorescent probes tailored for VOC detection across diverse platforms, highlighting their selectivity, sensitivity, and real-world applications. The use of AIE events has been justified for the development of selective and sensitive sensors for VOC detection. Furthermore, the present work investigates the various sensing mechanisms involved in predicting the AIE nature of the fluorophores. Additionally, we discuss existing challenges and propose future directions for designing next-generation AIE-based VOC sensors with enhanced specificity, stability, and practical usability. Coupled with the latest advancements, AIE-based fluorescence sensing of VOCs could contribute to the structural design of future smart VOC sensors that are highly efficient and effective.

挥发性有机化合物（VOCs）是许多日用产品中的重要成分，但由于其高毒性，即使是极少量，也会对公众健康和环境构成重大威胁。因此，开发一种可靠的方法，以高灵敏度和高选择性地跟踪这些有害蒸汽是至关重要的。在各种技术中，基于荧光的检测策略在实时定性和定量监测挥发性有机化合物方面表现出很高的效率。这些方法因其低成本和可移植性而具有吸引力，使其适合现场应用。本文综述了基于聚合诱导发射（AIE）的荧光探针的最新进展，这些荧光探针专门用于各种平台上的VOC检测，重点介绍了它们的选择性、灵敏度和实际应用。使用AIE事件已被证明是合理的选择性和敏感的传感器用于VOC检测的发展。此外，本研究还研究了预测荧光团AIE性质所涉及的各种传感机制。此外，我们讨论了现有的挑战，并提出了设计下一代基于ai的VOC传感器的未来方向，这些传感器具有更高的特异性、稳定性和实用性。结合最新进展，基于人工智能的VOCs荧光传感可以为未来高效、高效的智能VOC传感器的结构设计做出贡献。

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引用次数: 0

Recent development of surface treatment of polytetrafluoroethylene 聚四氟乙烯表面处理的最新进展

IF 2 4区化学 Q3 CHEMISTRY, ANALYTICAL

Analytical Sciences

Pub Date : 2025-11-25 DOI: 10.1007/s44211-025-00853-2

Ryoichi Ishimatsu

引用次数: 0

Development of surface-functionalized power-free microchip for breast cancer cell-derived extracellular vesicle detection. 用于乳腺癌细胞源性细胞外囊泡检测的表面功能化无功率微芯片的研制。

IF 2 4区化学 Q3 CHEMISTRY, ANALYTICAL

Analytical Sciences

Pub Date : 2025-11-20 DOI: 10.1007/s44211-025-00854-1

Ryo Ishihara, Ryo Matsui, Tadaaki Nakajima, Hinako Yokohari, Ren Ogata, Kotomi Katori, Kazuo Hosokawa, Mizuo Maeda, Akihiko Kikuchi

Extracellular vesicles (EVs) have emerged as promising biomarkers for liquid biopsies because of their responsiveness to various physiological conditions. However, their dynamic physical properties pose significant analytical challenges, which necessitates the development of novel detection methods. In this study, a portable surface-functionalized power-free microchip (SF-PF microchip) was developed that enables the detection of EVs without external pumps. In particular, the SF-PF microchip specifically detected EVs derived from metastatic breast cancer cells with a limit of detection of 5.0 × 10¹⁰ particles/mL. Moreover, the SF-PF microchip required only 2.0 µL of sample volume and completed the detection within approximately 20 min, offering a rapid and convenient approach for EV analysis. Furthermore, the SF-PF microchip successfully detected EVs in serum samples, which highlights its potential for clinical application in liquid biopsies.

细胞外囊泡（EVs）因其对各种生理条件的反应性而成为液体活检中有前途的生物标志物。然而，它们的动态物理性质给分析带来了重大挑战，这就需要开发新的检测方法。在这项研究中，开发了一种便携式表面功能化无电源微芯片（SF-PF微芯片），可以在没有外部泵的情况下检测电动汽车。特别是，SF-PF微芯片特异性检测转移性乳腺癌细胞衍生的ev，检测限为5.0 × 1010颗粒/mL。此外，SF-PF微芯片只需要2.0µL的样品体积，并在大约20分钟内完成检测，为EV分析提供了一种快速方便的方法。此外，SF-PF微芯片成功检测血清样本中的ev，这突出了其在液体活检中的临床应用潜力。

引用次数: 0

Sample pretreatment approaches for improving versatility and convenience in gas chromatographic analysis 提高气相色谱分析通用性和方便性的样品前处理方法

IF 2 4区化学 Q3 CHEMISTRY, ANALYTICAL

Analytical Sciences

Pub Date : 2025-11-10 DOI: 10.1007/s44211-025-00848-z

Yasuyuki Ishida

引用次数: 0

Biomineralized gold nanoparticle-based colorimetric sensing platform for ascorbic acid detection 基于生物矿化金纳米粒子的抗坏血酸检测比色传感平台。

IF 2 4区化学 Q3 CHEMISTRY, ANALYTICAL

Analytical Sciences

Pub Date : 2025-10-08 DOI: 10.1007/s44211-025-00852-3

Zhuqing Wang, Junyuan Zhang, Yuetong Ma, Mengran Zhang, Huixue Zhang, Bing Yang, Qiaorong Tang

Ascorbic acid (AA), a critical regulator of redox homeostasis, plays a vital role in disease prevention. Thus, the development of a rapid and precise detection method is urgently needed. In this work, a colorimetric sensing platform based on bioinspired mineralization-synthesized gold nanoparticles (AuNPs) was proposed for the visual and rapid detection of AA. The as-prepared AuNPs exhibited uniform morphology and excellent oxidase-like activity, efficiently catalyzing 3,3',5,5'-tetramethylbenzidine (TMB) oxidation to produce a characteristic 650 nm absorption peak. However, in the presence of AA, TMB oxidation was significantly inhibited due to the reducing property of AA. The color change was visually detectable within 5 min. The absorbance at 650 nm linearly decreased with the AA concentration in the range of 1–60 μM (R² = 0.999). The detection limit was estimated to 0.27 μM. Besides, the selectivity was excellent against common interfering species. The detection of AA in complex fruit samples was realized, and the recovery rate was 98.5–106.1%. Owing to the simplicity, cost-effectiveness, high sensitivity, and rapid response, this colorimetric sensing strategy provides new insights into the on-site monitoring of antioxidants in biological samples.

Graphical abstract

抗坏血酸（AA）是氧化还原稳态的关键调节因子，在疾病预防中起着重要作用。因此，迫切需要开发一种快速、精确的检测方法。本文提出了一种基于生物激发矿化合成金纳米粒子（AuNPs）的比色传感平台，用于视觉和快速检测AA。所制备的AuNPs具有均匀的形态和优异的类氧化酶活性，能有效催化3,3',5,5'-四甲基联苯胺（TMB）氧化，产生650 nm的特征吸收峰。然而，在AA存在下，由于AA的还原性，TMB的氧化被显著抑制。肉眼可在5分钟内检测到颜色变化。在650 nm处吸光度随AA浓度在1 ~ 60 μM范围内线性降低（R2 = 0.999）。检出限为0.27 μM。此外，对常见的干扰物质具有良好的选择性。实现了复杂水果样品中AA的检测，回收率为98.5 ~ 106.1%。由于简单、经济、高灵敏度和快速响应，这种比色传感策略为生物样品中抗氧化剂的现场监测提供了新的见解。

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引用次数: 0

Solid-phase extraction of ethyleneamines in river water prior to their determination by high-performance liquid chromatography with ultraviolet detection 采用高效液相色谱-紫外检测法对河水中乙胺进行固相萃取测定。

IF 2 4区化学 Q3 CHEMISTRY, ANALYTICAL

Analytical Sciences

Pub Date : 2025-10-02 DOI: 10.1007/s44211-025-00850-5

Azusa Yudo, Tomoko Kemmei, Shuji Kodama, Yoshinori Inoue, Shigehiro Kagaya

A method based on solid-phase extraction (SPE) has been developed for the preconcentration of three kinds of ethyleneamines (EAs) [ethylenediamine (EDA), diethylenetriamine (DETA), and triethylenetetramine (TETA)] in river water for their high-performance liquid chromatographic (HPLC) determination by using on-line complexation with Cu(II) ion and ultraviolet (UV) detection. EAs, which are protonated in acidic and neutral conditions, could be concentrated by using cation-exchange SPE. However, inorganic cations and humic substances, often present in river water, interfered with this preconcentration. Inorganic cations, such as Ca and Mg ions, compete with EAs in cation-exchange processes. This interference could be reduced by masking them with ethylenediaminetetraacetic acid. Humic substances, such as humic acid and fluvic acid, seem to be due to the electrostatic interaction of EAs with them in the sample solution. This interference was reduced by passing the sample solution through an anion-exchange cartridge before the cation-exchange SPE. The interacted EAs remained in the anion-exchange cartridge; they could be eluted by washing out the anion-exchange cartridge with diluted HCl. In this washing process, although fulvic acid was eluted at the same time, it could be removed by passing the eluate through a reversed-phase cartridge connected in a series. After removing these interferences, EAs could be extraced with the cation-exchange SPE and quantitatively eluted by passing Cu(II) solution through the cartridge. The proposed method of including the HPLC–UV combined with the SPE was available for the determination of EAs in river water at concentrations as low as 0.015 µM for EDA and DETA and 0.05 µM for TETA. The high recoveries (83%–103%) and repeatabilities (RSD 1.2%–4.3%) were obtained for EAs from two river water samples spiked with two different concentrations of EAs. The proposed method was applied to analyses of river water samples taken from four rivers in Toyama Prefecture, Japan.

Graphical Abstract

建立了基于固相萃取（SPE）的三种乙二胺[乙二胺（EDA）、二乙三胺（DETA）和三乙四胺（TETA）]在河水中预富集的高效液相色谱(HPLC) - Cu（II）离子在线络合和紫外（UV）检测方法。EAs在酸性和中性条件下均被质子化，可通过阳离子交换SPE进行富集。然而，无机阳离子和腐殖质，通常存在于河水，干扰这种预浓缩。无机阳离子，如Ca和Mg离子，在阳离子交换过程中与ea竞争。用乙二胺四乙酸掩盖可以减少这种干扰。腐植酸和流酸等腐植酸物质似乎是由于ea与样品溶液中的静电相互作用而产生的。在进行阳离子交换固相萃取之前，将样品溶液通过阴离子交换筒来减少这种干扰。相互作用的ea留在阴离子交换盒中；它们可以通过用稀释的盐酸冲洗阴离子交换筒来洗脱。在此洗涤过程中，虽然黄腐酸是同时洗脱的，但可以通过将洗脱液通过串联的反相滤筒除去。去除这些干扰后，可以用阳离子交换固相萃取萃取ea，并用Cu（II）溶液通过滤筒定量洗脱。建立的高效液相色谱-紫外联用固相萃取法可用于测定河水中EDA和DETA浓度低至0.015µM， TETA浓度低至0.05µM的EAs。两种不同浓度的ea在两种河流水样中均具有较高的回收率（83% ~ 103%）和重复性（RSD 1.2% ~ 4.3%）。所提出的方法应用于分析取自日本富山县四条河流的水样。

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引用次数: 0

Virus inactivation effect of hypochlorite solution containing nitrate ions 含硝酸盐离子的次氯酸盐溶液的病毒灭活效果。

IF 2 4区化学 Q3 CHEMISTRY, ANALYTICAL

Analytical Sciences

Pub Date : 2025-10-01 DOI: 10.1007/s44211-025-00831-8

Erina Satake, Maho Ishiyama, Masaki Sekiguchi, Atsushi Sasaki, Shigekazu Yano, Masatoshi Endo

Hypochlorous acid (HClO) is widely used as a disinfectant due to its strong oxidizing power, but its efficacy is highly dependent on pH and decreases under neutral to alkaline conditions. In addition, its high concentration poses safety and environmental concerns. In this study, we investigated the enhancement of virucidal activity at lower HClO concentrations through the formation of monochloramine (NH₂Cl) by supplementing with ammonium-form nitrogen (NH₄⁺–N) or nitrate-form nitrogen (NO₃⁻–N) under different pH conditions. Using bacteriophage φ6 as an enveloped virus model, plaque assays revealed that virus inactivation was most effective at pH 8 when 15–20 ppm of NH₄⁺–N was added to 30 ppm HClO solution, with over 90% reduction in infectivity. Under acidic conditions (pH 6), the formation of NH₂Cl was limited due to the rapid consumption of free chlorine and insufficient generation of NH₃, resulting in reduced virucidal efficacy; however, increasing the amount of NH₄⁺–N led to improved inactivation effects. In contrast, at pH 8, NH₂Cl formation was enhanced, contributing to improved disinfection efficiency. Similar effects were observed when NO₃⁻–N was used, likely due to its reduction to NH₄⁺ in the presence of organic matter. These results demonstrate that optimizing pH and nitrogen sources can improve the efficacy of low-concentration HClO solutions by generating NH₂Cl in situ, offering a safer and more sustainable approach to viral disinfection.

Graphical abstract

次氯酸（HClO）因其强大的氧化能力而被广泛用作消毒剂，但其效果高度依赖于pH值，在中性至碱性条件下会下降。此外，它的高浓度引起了安全和环境问题。在本研究中，我们研究了在不同的pH条件下，通过补充铵态氮（NH4+-N）或硝酸盐态氮（NO3——N）形成一氯胺（NH2Cl），在低HClO浓度下增强病毒的杀病毒活性。以φ6噬菌体为包膜病毒模型，在pH为8时，将15-20 ppm的NH4+-N加入到30 ppm的HClO溶液中，病毒灭活效果最好，传染性降低90%以上。在酸性条件下（pH 6），由于游离氯的快速消耗和NH₃的生成不足，NH2Cl的形成受到限制，导致杀病毒效果降低；然而，增加NH4+-N的用量可以改善失活效果。相反，在pH为8时，NH2Cl的形成增强，有助于提高消毒效率。当使用NO3—N时，观察到类似的效果，可能是由于它在有机物存在下还原成NH₄⁺。这些结果表明，优化pH和氮源可以通过原位生成NH2Cl来提高低浓度HClO溶液的消毒效果，为病毒消毒提供了一种更安全、更可持续的方法。

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引用次数: 0

Advances in microfluidic chip technology for cell analysis 用于细胞分析的微流控芯片技术进展。

IF 2 4区化学 Q3 CHEMISTRY, ANALYTICAL

Analytical Sciences

Pub Date : 2025-09-30 DOI: 10.1007/s44211-025-00851-4

Luxi Shu, Yan Zhang

Microfluidic chips play a crucial role in the field of cell analysis. These miniature chips integrate seamlessly across key cell analysis stages, including sample preparation, cell culture, sorting, lysis, and detection. They possess many advantages such as miniaturization, integration, automation, and portability. The size of their microchannels is comparable to that of cells, thus enabling research to be conducted at the single-cell or even subcellular organelle level. They can also simulate the physiological conditions in vivo, conduct non-destructive or minimally destructive detections, and meet the requirements of high-throughput cell analysis, which is beneficial for parallel operations and continuous analysis. This article reviews the technological progress of microfluidic chips, focusing on three major directions: cell sorting and enrichment, single-cell analysis, and dynamic microenvironment simulation. The article also analyzes the challenges faced by this technology, such as cell damage control, handling of multicellular heterogeneity, data interpretation, etc., and proposes coping strategies such as the development of new biomaterials, multimodal integration technology, and artificial intelligence assistance. By combining cutting-edge technologies such as nanotechnology, 3D printing, and organoid culture, the functions of microfluidic chips can be further expanded to enable the simulation and analysis of more complex biological systems and provide important technical support for cell biology research and clinical translation.

Graphical Abstract

微流控芯片在细胞分析领域起着至关重要的作用。这些微型芯片无缝集成在关键的细胞分析阶段，包括样品制备，细胞培养，分选，裂解和检测。它们具有许多优点，如小型化、集成化、自动化和可移植性。它们的微通道大小与细胞相当，因此可以在单细胞甚至亚细胞细胞器水平上进行研究。还可以模拟体内生理条件，进行无损或最小破坏性检测，满足高通量细胞分析的要求，有利于并行操作和连续分析。本文综述了微流控芯片的技术进展，重点介绍了细胞分选富集、单细胞分析和动态微环境模拟三个主要方向。文章还分析了该技术面临的挑战，如细胞损伤控制、多细胞异质性处理、数据解释等，并提出了开发新型生物材料、多模态集成技术、人工智能辅助等应对策略。通过结合纳米技术、3D打印、类器官培养等前沿技术，可以进一步扩展微流控芯片的功能，使其能够模拟和分析更复杂的生物系统，为细胞生物学研究和临床翻译提供重要的技术支持。

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引用次数: 0

Recent topics in X-ray absorption spectroscopy: integrating theory, instrumentation, and applications x射线吸收光谱学的最新课题：集成理论、仪器和应用

IF 2 4区化学 Q3 CHEMISTRY, ANALYTICAL

Analytical Sciences

Pub Date : 2025-09-26 DOI: 10.1007/s44211-025-00840-7

Daitaro Ishikawa

引用次数: 0

Rolling circle transcription-based fluorescent aptasensor for highly sensitive and onsite detection of microcystin-LR 基于滚动环转录的荧光适体传感器，用于高灵敏度和现场检测微囊藻毒素。

IF 2 4区化学 Q3 CHEMISTRY, ANALYTICAL

Analytical Sciences

Pub Date : 2025-09-19 DOI: 10.1007/s44211-025-00839-0

Shuran Yang, Yuling Xu, Cheng Zhang, Ke Xu, Zhiyong Hu, Zhiqiang Xia

Onsite and sensitive detection of microcystin-LR (MC-LR) is crucial for monitoring environmental pollution and protecting human health, particularly in the context of increasing cyanobacterial harmful algal blooms (CyanoHABs). Herein, this study reports a rolling circle transcription (RCT)-based fluorescent aptasensor designed for rapid and quantitative onsite detection of MC-LR in real water samples. Aptamers specific to MC-LR were immobilized onto magnetic beads (MBs) and hybridized with blocker complementary DNA strands to form the MB aptasensor complex. Upon recognition and binding of MC-LR molecules in water samples, the blocker DNA strands were displaced and subsequently cyclized into circular DNA structures via T4 DNA ligase-mediated ligation. These circular DNA templates initiated RCT, generating abundant fluorescent Mango RNA reporters. The resulting fluorescent signals exhibited a robust positive correlation with MC-LR concentration, with a detection limit of 39 pM and a linear range from 0.01 to 3000 nM, demonstrating high sensitivity and specificity. The aptasensor showed excellent specificity against structural analogs and common interferents. Recovery tests in Haihe River samples yielded rates between 98.33% and 101.17%, with relative standard deviations below 5%. This sensing platform offers a simple operational procedure, minimal sample preparation, and excellent applicability for real environmental water monitoring. Thus, the developed RCT-based aptasensor provides a promising, cost-effective alternative for onsite MC-LR detection and contributes significantly to environmental safety and public health management.

Graphical abstract

微囊藻毒素lr （MC-LR）的现场灵敏检测对于监测环境污染和保护人类健康至关重要，特别是在蓝藻有害藻华（CyanoHABs）增加的背景下。本研究报道了一种基于滚动环转录（RCT）的荧光适体传感器，用于快速定量现场检测真实水样中的MC-LR。将MC-LR特异性适配体固定在磁珠（MB）上，并与阻滞剂互补DNA链杂交，形成MB适配体传感器复合物。在水样中识别和结合MC-LR分子后，阻断剂DNA链被移位，随后通过T4 DNA连接酶介导的连接环化成环状DNA结构。这些环状DNA模板启动了RCT，产生了大量的荧光芒果RNA报告基因。结果表明，荧光信号与MC-LR浓度呈正相关，检测限为39 pM，线性范围为0.01 ~ 3000 nM，具有较高的灵敏度和特异性。该配体传感器对结构类似物和常见干扰物具有良好的特异性。海河样品回收率为98.33% ~ 101.17%，相对标准偏差小于5%。该传感平台提供了一个简单的操作程序，最少的样品制备，和优秀的适用性，真正的环境水监测。因此，所开发的基于rct的感应传感器为现场MC-LR检测提供了一种有前途的、具有成本效益的替代方案，并为环境安全和公共卫生管理做出了重大贡献。

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