Genetic diversification of allohexaploid Brassica hybrids (AABBCC) using a fertile octoploid with excessive C genome set (AABBCCCC).

IF 3.6 3区 生物学 Q1 PLANT SCIENCES Planta Pub Date : 2024-08-13 DOI:10.1007/s00425-024-04497-w
Teppei Shinke, Akira Yamazaki, Soraha Nakamura, Hiroshi Kudoh, Munetaka Hosokawa
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Abstract

Main conclusion: Using octoploid somatic hybrids with excessive C genome sets, AABBCCCC, a diverse allohexaploid, AABBCC, was produced by C genome reduction through subsequent crossing with various AABB cultivars. Even when somatic hybrids are produced, the plants that are produced are rarely in themselves an innovative crop. In this study, we used somatic hybrids of Brassica juncea (AABB) and B. oleracea (CC) as model cases for the genetic diversification of the somatic hybrids. One cell of 'Akaoba Takana' (B. juncea) and two cells of 'Snow Crown' (B. oleracea) were fused to create several somatic hybrids with excessive C genomes, AABBCCCC. Using AABBCCCC somatic hybrids as mother plants and crossing with 'Akaoba Takana', the AABBCC progenies were generated. When these AABBCC plants were self-fertilized, and flow cytometric (FCM) analysis was performed on the next generations, differences in the relative amount of genome size variation were observed, depending on the different AABBCCCC parents used for AABBCC creation. Further self-progeny was obtained for AABBCC plants with a theoretical allohexaploid DNA index by FCM. However, as the DNA indices of the progeny populations varied between plants used and aneuploid individuals still occurred in the progeny populations, it was difficult to say that the allohexaploid genome was fully stabilized. Next, to obtain genetic diversification of the allohexaploid, different cultivars of B. juncea were crossed with AABBCCCC, resulting in diverse AABBCC plants. Genetic diversity can be further expanded by crossbreeding plants with different AABBCC genome sets. Although genetic stability is necessary to ensure in the later generations, the results obtained in this study show that the use of somatic hybrids with excess genomes is an effective strategy for creating innovative crops.

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利用具有过量 C 基因组的可育八倍体(AABBCCCC)实现异源六倍体芸薹属杂交种(AABBCC)的遗传多样化。
主要结论:利用具有过多 C 基因组的八倍体体细胞杂交种 AABBCCCC,通过减少 C 基因组,随后与各种 AABB 栽培品种杂交,产生了多样化的异源六倍体 AABBCC。即使产生了体细胞杂交种,所培育出的植株本身也很少能成为创新作物。在本研究中,我们以芥蓝的体细胞杂交种(AABB)和甘蓝的体细胞杂交种(CC)作为体细胞杂交种遗传多样化的示范案例。将'Akaoba Takana'(芥蓝)的一个细胞和'Snow Crown'(油菜)的两个细胞融合,产生了多个C基因组过多的体细胞杂交种,即AABBCCCC。以 AABBCCCC 体细胞杂交种为母本,与'Akaoba Takana'杂交,产生了 AABBCC 后代。对这些 AABBCC 植物进行自交,并对下一代进行流式细胞仪(FCM)分析时,发现基因组大小的相对变异量存在差异,这取决于创建 AABBCC 时使用的不同 AABBCC 亲本。通过 FCM 分析,AABBCC 植物进一步获得了自交后代,其 DNA 指数理论上为异源六倍体。然而,由于不同植株的后代群体 DNA 指数不同,而且后代群体中仍有非整倍体个体,因此很难说异源六倍体基因组已完全稳定。接下来,为了获得异源六倍体的遗传多样性,将不同的君子兰栽培品种与 AABBCCCC 进行杂交,得到了多样性的 AABBCC 植株。通过杂交具有不同 AABBCC 基因组的植物,可以进一步扩大遗传多样性。虽然必须确保后代的遗传稳定性,但本研究的结果表明,使用基因组过剩的体细胞杂交种是创造创新作物的有效策略。
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来源期刊
Planta
Planta 生物-植物科学
CiteScore
7.20
自引率
2.30%
发文量
217
审稿时长
2.3 months
期刊介绍: Planta publishes timely and substantial articles on all aspects of plant biology. We welcome original research papers on any plant species. Areas of interest include biochemistry, bioenergy, biotechnology, cell biology, development, ecological and environmental physiology, growth, metabolism, morphogenesis, molecular biology, new methods, physiology, plant-microbe interactions, structural biology, and systems biology.
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