Synergistic effects of Bacillus amyloliquefaciens strains CBa_BFL2 and CBa_RA37 from the desert triangular spurge on the bacteria wilt disease in tomato

IF 3.9 2区农林科学 Q1 AGRONOMY Plant and Soil Pub Date : 2024-08-12 DOI:10.1007/s11104-024-06878-2

Pierre Eke, Diane Yimta Youmbi, Prasad Sahu Kuleshwar, Lanvin Rochal Kepngop Kouokap, Vanessa Nya Dinango, Aundy Kumar

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Abstract

Background and aim

Consortia-based inoculants, such as the microbiome method, are advocated over individual biocontrol agents (BCAs), owing to partner reinforcement and rapid rhizospheric establishment. Herein, we screened the sole and dual suppressive effects of selected desert spurge (Euphorbia antiquorum) derived Bacillus spp. vis-à-vis Ralstonia solanacearum.

Methods

The conventional agar diffusion method was used to examine the BCAs' capacity to release antibiotics. Thereafter, the compatibility, the ability to form biofilm and the phytotoxicity of the BCAs towards tobacco leaves were screened. The Box-PCR fingerprinting technique was adopted to ascertain the endophytism of the BCAs, and the wilt suppression test followed by the specific activity of defence-related enzymes was performed.

Results

R. solanacearum was completely inhibited by the extracts from Bacillus velezensis CBv_BE1, Bacillus amyloliquefaciens CBa_BFL2, and Bacillus amyloliquefaciens CBa_RA37 at 0.625 mg/mL. The outstanding candidates could form biofilm and colonize into tomato seedlings, with a plateau at log10 CFU = 4.29/g fresh weight. The consortium from CBa_BFL2 and CBa_RA37 (CBa_BFL2/CBa_RA37) yielded vigorous plants (up to a 319% increase in biomass). Likewise, a 90% and 89% reduction in wilt incidence and severity was recorded by the CBa_BFL2-CBa_RA37 combination. The Guaiacol peroxidases (GPX), phenylalanine ammonia lyase (PAL), and superoxide dismutase (SOD) specific activity were significantly and inversely correlated with wilt severity via Pearson's test. This alludes to a greater innate defence system in tomatoes in response to bacterization.

Conclusions

Our data suggest that tomato priming with a consortium of B. amyloliquefaciens CBa_BFL2 and CBa_RA37 resulted in vigorous and healthier tomato seedlings as referred to their sole effects, with altered activities of key plant distressing enzymes. However, further studies are warranted to reveal its full potential as a pioneering alternative to agrochemicals for the control of bacterial wilt.

Graphical Abstract

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来自沙漠三角刺的淀粉芽孢杆菌菌株 CBa_BFL2 和 CBa_RA37 对番茄细菌性枯萎病的协同作用

背景和目的与单个生物控制剂（BCA）相比，微生物组法等基于群落的接种剂因其伙伴强化和根瘤层快速建立而受到推崇。在此，我们筛选了选定的沙漠刺芹（Euphorbia antiquorum）衍生芽孢杆菌属（Bacillus spp.）对茄属雷氏菌（Ralstonia solanacearum）的单一和双重抑制作用。随后，对 BCAs 的相容性、形成生物膜的能力以及对烟草叶片的植物毒性进行了筛选。采用 Box-PCR 指纹图谱技术确定了 BCA 的内生性，并进行了枯萎病抑制试验和防御相关酶的特异性活性试验。结果 0.625 mg/mL 的枯草芽孢杆菌 CBv_BE1、淀粉芽孢杆菌 CBa_BFL2 和淀粉芽孢杆菌 CBa_RA37 的提取物可完全抑制茄碱菌。优秀的候选菌株可在番茄幼苗上形成生物膜并定植，在 log10 CFU = 4.29/g 鲜重时达到高峰。由 CBa_BFL2 和 CBa_RA37 组成的联合体（CBa_BFL2/CBa_RA37）能培育出生命力旺盛的植株（生物量最多可增加 319%）。同样，CBa_BFL2-CBa_RA37 组合的枯萎病发病率和严重程度也分别降低了 90% 和 89%。通过皮尔逊检验，愈创木酚过氧化物酶（GPX）、苯丙氨酸氨裂解酶（PAL）和超氧化物歧化酶（SOD）的特异性活性与枯萎病的严重程度显著成反比。结论我们的数据表明，与单独使用 B. amyloliquefaciens CBa_BFL2 和 CBa_RA37 的效果相比，使用 B. amyloliquefaciens CBa_BFL2 和 CBa_RA37 联合体对番茄进行底物处理后，番茄幼苗更健壮，关键植物有害酶的活性也有所改变。然而，要充分揭示其作为农用化学品替代品控制细菌性萎蔫病的潜力，还需要进一步的研究。

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来源期刊

Plant and Soil 农林科学-农艺学

CiteScore

8.20

自引率

8.20%

发文量

543

审稿时长

2.5 months

期刊介绍： Plant and Soil publishes original papers and review articles exploring the interface of plant biology and soil sciences, and that enhance our mechanistic understanding of plant-soil interactions. We focus on the interface of plant biology and soil sciences, and seek those manuscripts with a strong mechanistic component which develop and test hypotheses aimed at understanding underlying mechanisms of plant-soil interactions. Manuscripts can include both fundamental and applied aspects of mineral nutrition, plant water relations, symbiotic and pathogenic plant-microbe interactions, root anatomy and morphology, soil biology, ecology, agrochemistry and agrophysics, as long as they are hypothesis-driven and enhance our mechanistic understanding. Articles including a major molecular or modelling component also fall within the scope of the journal. All contributions appear in the English language, with consistent spelling, using either American or British English.