Ortho-Vanillin Ameliorates Spinetoram-Induced Oxidative Stress in the Silkworm Bombyx mori: Biochemical and In Silico Insights.

IF 1.4 3区 农林科学 Q2 ENTOMOLOGY Neotropical Entomology Pub Date : 2024-10-01 Epub Date: 2024-08-14 DOI:10.1007/s13744-024-01191-0
Hashim Ashraf, Pallavi Agrawal, Neha Singh, Nikhil Maheshwari, Ayesha Qamar
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Abstract

This study investigates the toxic effects of the insecticide spinetoram on the model organism Bombyx mori (Linnaeus) and explores the potential ameliorative properties of O-Vanillin. Sub-lethal concentrations of spinetoram were given to silkworm larvae via oral feed, resulting in reduced body weight, larval length, and impaired cocoon characteristics. A study of the enzymatic and non-enzymatic antioxidants revealed oxidative stress in the gut, fat body, and silk gland tissues, characterized by decreased antioxidants and increased lipid peroxidation. However, post-treatment with O-Vanillin effectively mitigated these toxic effects, preserving antioxidant capacities and preventing lipid peroxidation. Additionally, O-Vanillin prevented the loss of body weight and improved cocoon characteristics. At the histological level, spinetoram exposure caused mild histological damage in the gut, fat body, and silk gland. However, O-Vanillin post-treatment had ameliorative effects and mitigated the histological damages. To delve deeper into the mechanism of amelioration of O-Vanillin, in silico studies were used to study the interaction between an important xenobiotic metabolism protein of the Bombyx mori, i.e., Cytochrome p450, specifically CYP9A19, and O-Vanillin. We performed blind molecular docking followed by molecular dynamic simulation, and the results demonstrated stable binding interactions between O-Vanillin and CYP9A19, a cytochrome P450 protein in silkworm, belonging to the subfamily CYP9A, suggesting a potential role for O-vanillin in modulating xenobiotic metabolism.

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Ortho-Vanillin Ameliorates Spinetoram-Induced Oxidative Stress in the Silkworm Bombyx mori: Biochemical and In Silico Insights.
本研究调查了杀虫剂斯皮内酯对模式生物蚕蛾(林奈)的毒性作用,并探讨了 O-香兰素的潜在改善特性。通过口服给蚕幼虫喂食亚致死浓度的辛硫磷,会导致其体重、幼虫体长减少,蚕茧特性受损。对酶和非酶抗氧化剂的研究表明,肠道、脂肪体和丝腺组织中存在氧化应激,其特征是抗氧化剂减少和脂质过氧化增加。然而,用 O-香兰素进行后处理可有效减轻这些毒性效应,保持抗氧化能力并防止脂质过氧化。此外,O-香兰素还能防止体重下降并改善茧的特性。在组织学层面,暴露于辛弗林会对肠道、脂肪体和丝腺造成轻微的组织学损伤。然而,O-香兰素后处理具有改善作用,减轻了组织损伤。为了深入探讨 O-香兰素的改善机制,我们使用了硅学研究来研究一种重要的森蝇异生物代谢蛋白(即细胞色素 p450,特别是 CYP9A19)与 O-香兰素之间的相互作用。结果表明,O-香兰素与属于 CYP9A 亚家族的家蚕细胞色素 P450 蛋白 CYP9A19 之间存在稳定的结合相互作用,这表明 O-香兰素在调节异生物代谢方面具有潜在的作用。
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来源期刊
Neotropical Entomology
Neotropical Entomology 生物-昆虫学
CiteScore
3.30
自引率
5.60%
发文量
69
审稿时长
6-12 weeks
期刊介绍: Neotropical Entomology is a bimonthly journal, edited by the Sociedade Entomológica do Brasil (Entomological Society of Brazil) that publishes original articles produced by Brazilian and international experts in several subspecialties of entomology. These include bionomics, systematics, morphology, physiology, behavior, ecology, biological control, crop protection and acarology.
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