Two dwarfing genes Rht-B1b and Rht-D1b show pleiotropic effects on grain protein content in bread wheat (Triticum aestivum L.).

IF 4.4 1区农林科学 Q1 AGRONOMY Theoretical and Applied Genetics Pub Date : 2024-08-14 DOI:10.1007/s00122-024-04713-x

Wenjing Hu, Di Wu, Dongshen Li, Xiaoming Cheng, Zunjie Wang, Die Zhao, Jizeng Jia

{"title":"Two dwarfing genes Rht-B1b and Rht-D1b show pleiotropic effects on grain protein content in bread wheat (Triticum aestivum L.).","authors":"Wenjing Hu, Di Wu, Dongshen Li, Xiaoming Cheng, Zunjie Wang, Die Zhao, Jizeng Jia","doi":"10.1007/s00122-024-04713-x","DOIUrl":null,"url":null,"abstract":"<p><strong>Key message: </strong>Five QTL for wheat grain protein content were identified, and the effects of two dwarfing genes Rht-B1b and Rht-D1b on grain protein content were validated in multiple populations. Grain protein content (GPC) plays an important role in wheat quality. Here, a recombinant inbred line (RIL) population derived from a cross between Yangmai 12 (YM12) and Yanzhan 1 (YZ1) was used to identify quantitative trait loci (QTL) for GPC. Two hundred and five RILs and their parents were grown in three years in randomized complete blocks each with two replications, and genotyped using the wheat 55 K SNP array. Five QTL were identified for GPC on chromosomes 1A, 1B, 2D, 4B, and 4D. Notably, QGpc.yaas-4B (co-located with Rht-B1) and QGpc.yaas-4D (co-located with Rht-D1) were consistently detected across all experiments and best linear unbiased estimating, accounting for 6.61-8.39% and 6.05-10.21% of the phenotypic variances, respectively. The effects of these two dwarfing alleles Rht-B1b and Rht-D1b on reducing GPC and plant height were validated in two additional RIL populations and one natural population. This study lays a foundation for further investigating the effects of dwarfing genes Rht-B1b and Rht-D1b on wheat GPC.</p>","PeriodicalId":22955,"journal":{"name":"Theoretical and Applied Genetics","volume":null,"pages":null},"PeriodicalIF":4.4000,"publicationDate":"2024-08-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Theoretical and Applied Genetics","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.1007/s00122-024-04713-x","RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"AGRONOMY","Score":null,"Total":0}

引用次数: 0

Abstract

Key message: Five QTL for wheat grain protein content were identified, and the effects of two dwarfing genes Rht-B1b and Rht-D1b on grain protein content were validated in multiple populations. Grain protein content (GPC) plays an important role in wheat quality. Here, a recombinant inbred line (RIL) population derived from a cross between Yangmai 12 (YM12) and Yanzhan 1 (YZ1) was used to identify quantitative trait loci (QTL) for GPC. Two hundred and five RILs and their parents were grown in three years in randomized complete blocks each with two replications, and genotyped using the wheat 55 K SNP array. Five QTL were identified for GPC on chromosomes 1A, 1B, 2D, 4B, and 4D. Notably, QGpc.yaas-4B (co-located with Rht-B1) and QGpc.yaas-4D (co-located with Rht-D1) were consistently detected across all experiments and best linear unbiased estimating, accounting for 6.61-8.39% and 6.05-10.21% of the phenotypic variances, respectively. The effects of these two dwarfing alleles Rht-B1b and Rht-D1b on reducing GPC and plant height were validated in two additional RIL populations and one natural population. This study lays a foundation for further investigating the effects of dwarfing genes Rht-B1b and Rht-D1b on wheat GPC.

Abstract Image

查看原文

微信好友朋友圈 QQ好友复制链接

本刊更多论文

两个矮化基因Rht-B1b和Rht-D1b对面包小麦（Triticum aestivum L.）谷物蛋白质含量具有多效性。

关键信息：鉴定了小麦籽粒蛋白质含量的五个 QTL，并在多个群体中验证了两个矮化基因 Rht-B1b 和 Rht-D1b 对籽粒蛋白质含量的影响。谷物蛋白质含量（GPC）对小麦品质起着重要作用。本文利用扬麦12号（YM12）和烟占1号（YZ1）杂交产生的重组近交系（RIL）群体来鉴定谷粒蛋白含量的数量性状位点（QTL）。对 255 个 RIL 及其亲本进行了三年的随机完全区组种植，每个区组两次重复，并使用小麦 55 K SNP 阵列进行了基因分型。在 1A、1B、2D、4B 和 4D 染色体上发现了五个 GPC QTL。值得注意的是，QGpc.yaas-4B（与 Rht-B1 共定位）和 QGpc.yaas-4D（与 Rht-D1 共定位）在所有实验中都被一致检测到，并获得最佳线性无偏估计，分别占表型变异的 6.61%-8.39%和 6.05%-10.21%。这两个矮化等位基因 Rht-B1b 和 Rht-D1b 对降低 GPC 和株高的影响在另外两个 RIL 群体和一个自然群体中得到了验证。这项研究为进一步研究矮化基因 Rht-B1b 和 Rht-D1b 对小麦 GPC 的影响奠定了基础。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文去求助

来源期刊

Theoretical and Applied Genetics 生物-农艺学

CiteScore

9.60

自引率

7.40%

发文量

241

审稿时长

2.3 months

期刊介绍： Theoretical and Applied Genetics publishes original research and review articles in all key areas of modern plant genetics, plant genomics and plant biotechnology. All work needs to have a clear genetic component and significant impact on plant breeding. Theoretical considerations are only accepted in combination with new experimental data and/or if they indicate a relevant application in plant genetics or breeding. Emphasizing the practical, the journal focuses on research into leading crop plants and articles presenting innovative approaches.