Circ-PITX1 promotes non-small-cell lung cancer progression through regulating ETS1 expression via miR-615-5p.

IF 2.3 3区 医学 Q3 ONCOLOGY Thoracic Cancer Pub Date : 2024-09-01 Epub Date: 2024-08-13 DOI:10.1111/1759-7714.15414
Yang Guo, Jianfang Pan, Xiaofei Gao, Yan Zheng
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Abstract

Background: Circular RNAs (circRNAs), produced by reverse splicing, act as important players in human cancers. We aimed to assess the biological functions of circRNA pituitary homeobox 1 (circ-PITX1) in non-small-cell lung cancer (NSCLC).

Methods: qRT-PCR was employed to determine RNA expression. Biological behaviors of NSCLC cells were assessed by CCK-8, colony formation, EdU assay, flow cytometry, wound healing, and transwell assays. Glutamine catabolism was examined via the measurement of glutamine consumption, α-ketoglutarate levels, as well as ATP levels. Protein levels were detected by western blot assays. Dual-luciferase reporter assay and RNA immunoprecipitation (RIP) assay were performed to reveal the mechanism responsible for circ-PITX1 regulating NSCLC cell malignancy. The murine xenograft model was established to investigate circ-PITX1's effect on tumor formation.

Results: Circ-PITX1 was overexpressed in NSCLC tissue samples and cells. Its low expression repressed NSCLC cell proliferation and motility. Moreover, our data revealed its downregulation inhibited glutamine catabolism and tumor formation and promoted cell apoptosis. In addition, circ-PITX1 bound to miR-615-5p, and its inhibitory effect on tumor cellular behaviors could be reversed after decreasing miR-615-5p expression. The miRNA targeted E26 transformation specific-1 (ETS1), whose upregulation abolished miR-615-5p overexpression-induced effects in NSCLC cells. Furthermore, circ-PITX1 positively modulated ETS1 production through interaction with miR-615-5p.

Conclusion: Circ-PITX1 facilitated NSCLC progression via modulating miR-615-5p/ETS1 pathway.

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Circ-PITX1 通过 miR-615-5p 调控 ETS1 的表达,从而促进非小细胞肺癌的进展。
背景:由反向剪接产生的环状 RNA(circRNA)在人类癌症中扮演着重要角色。我们旨在评估环状 RNA 垂体同工酶 1(circ-PITX1)在非小细胞肺癌(NSCLC)中的生物学功能。通过 CCK-8、集落形成、EdU 试验、流式细胞术、伤口愈合和透孔试验评估 NSCLC 细胞的生物学行为。通过测量谷氨酰胺消耗量、α-酮戊二酸水平以及 ATP 水平来检测谷氨酰胺分解代谢。蛋白质水平通过西部印迹检测法进行检测。为了揭示circ-PITX1调控NSCLC细胞恶性程度的机制,还进行了双荧光素酶报告实验和RNA免疫沉淀(RIP)实验。建立了小鼠异种移植模型,研究 circ-PITX1 对肿瘤形成的影响:结果:Circ-PITX1在NSCLC组织样本和细胞中过表达。结果:Circ-PITX1 在 NSCLC 组织样本和细胞中过表达,其低水平表达抑制了 NSCLC 细胞的增殖和运动。此外,我们的数据显示,下调其表达可抑制谷氨酰胺分解和肿瘤形成,并促进细胞凋亡。此外,circ-PITX1与miR-615-5p结合,降低miR-615-5p的表达后,其对肿瘤细胞行为的抑制作用可被逆转。该miRNA靶向E26转化特异性-1(ETS1),ETS1的上调可消除miR-615-5p过表达对NSCLC细胞的影响。此外,circ-PITX1通过与miR-615-5p的相互作用积极调节ETS1的产生:Circ-PITX1通过调节miR-615-5p/ETS1通路促进了NSCLC的进展。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Thoracic Cancer
Thoracic Cancer ONCOLOGY-RESPIRATORY SYSTEM
CiteScore
5.20
自引率
3.40%
发文量
439
审稿时长
2 months
期刊介绍: Thoracic Cancer aims to facilitate international collaboration and exchange of comprehensive and cutting-edge information on basic, translational, and applied clinical research in lung cancer, esophageal cancer, mediastinal cancer, breast cancer and other thoracic malignancies. Prevention, treatment and research relevant to Asia-Pacific is a focus area, but submissions from all regions are welcomed. The editors encourage contributions relevant to prevention, general thoracic surgery, medical oncology, radiology, radiation medicine, pathology, basic cancer research, as well as epidemiological and translational studies in thoracic cancer. Thoracic Cancer is the official publication of the Chinese Society of Lung Cancer, International Chinese Society of Thoracic Surgery and is endorsed by the Korean Association for the Study of Lung Cancer and the Hong Kong Cancer Therapy Society. The Journal publishes a range of article types including: Editorials, Invited Reviews, Mini Reviews, Original Articles, Clinical Guidelines, Technological Notes, Imaging in thoracic cancer, Meeting Reports, Case Reports, Letters to the Editor, Commentaries, and Brief Reports.
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