Molecular basis of camphor repellency in Hyphantria cunea

IF 4.2 1区农林科学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY Pesticide Biochemistry and Physiology Pub Date : 2024-08-02 DOI:10.1016/j.pestbp.2024.106069

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Abstract

The plant-derived camphor has been used as a natural insect repellent against various insects for >500 years. However, the repellency mechanism behind camphor remains less understood. In this study, we aimed to identify the camphor receptor in Hyphantria cunea by deorphanizing 7 odorant receptors (ORs). The results showed that HcunOR46 is narrowly tuned to Camphor and is only conserved within the family Noctuidae. Further analysis through behavioral and electroantennograms (EAG) assays indicated that H. cunea adults are more sensitive to camphor than larvae, both behaviorally and electrophysiologically. This difference may be due to the lower expression of HcunOR46 at the larval stage. Additionally, a feeding assay indicated that camphor repellency could be related to camphor toxicity to larvae, with the lethal concentration 50 (LC₅₀) value of 69.713 μg/μL. These results suggest that H. cunea may detect camphor through a distinct olfactory pathway from Culicinae mosquitos, providing a novel camphor-based pest management strategy for H. cunea.

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Cunea 象鼻虫拒樟脑的分子基础

从植物中提取的樟脑被用作天然驱虫剂来对付各种昆虫已有 500 年的历史。然而，人们对樟脑的驱虫机制仍然知之甚少。在这项研究中，我们通过对 7 种气味受体（ORs）进行非形态化，旨在鉴定楔齿象（Hyphantria cunea）的樟脑受体。结果表明，HcunOR46对樟脑的调谐范围很窄，而且仅在夜蛾科内保守。通过行为和电图（EAG）测定进行的进一步分析表明，胭脂虫成虫在行为和电生理方面都比幼虫对樟脑更敏感。这种差异可能是由于幼虫阶段 HcunOR46 的表达量较低。此外，喂食试验表明，樟脑的驱避性可能与樟脑对幼虫的毒性有关，致死浓度 50（LC50）值为 69.713 μg/μL。这些结果表明，锹形目蚊子可能通过与秃头蝇科蚊子不同的嗅觉途径来检测樟脑，这为锹形目蚊子提供了一种基于樟脑的新型害虫管理策略。

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来源期刊

Pesticide Biochemistry and Physiology 生物-昆虫学

CiteScore

7.00

自引率

8.50%

发文量

238

审稿时长

4.2 months

期刊介绍： Pesticide Biochemistry and Physiology publishes original scientific articles pertaining to the mode of action of plant protection agents such as insecticides, fungicides, herbicides, and similar compounds, including nonlethal pest control agents, biosynthesis of pheromones, hormones, and plant resistance agents. Manuscripts may include a biochemical, physiological, or molecular study for an understanding of comparative toxicology or selective toxicity of both target and nontarget organisms. Particular interest will be given to studies on the molecular biology of pest control, toxicology, and pesticide resistance. Research Areas Emphasized Include the Biochemistry and Physiology of: • Comparative toxicity • Mode of action • Pathophysiology • Plant growth regulators • Resistance • Other effects of pesticides on both parasites and hosts.