{"title":"Outdoor semi-continuous cultivation of Synechococcus sp. for enhanced carotenoid production","authors":"","doi":"10.1016/j.algal.2024.103657","DOIUrl":null,"url":null,"abstract":"<div><p>The carotenoid productivity of a euryhaline <em>Synechococcus</em> sp. was studied in indoor and outdoor conditions. Indoor experiments revealed that the strain could maintain growth and carotenoid accumulation within a salinity range of 2.5–8.0 % NaCl. In an indoor two phase semi-continuous cultivation in PBRs, every three days, 50 % of the culture was replaced with fresh growth media in phase one, whereas the removed culture was grown for another three days in the second phase to enhance the carotenoid yield. Biomass and carotenoid productivity of the strain in the second phase were similar in five consecutive cultivation cycles. Next, the same two-phase cultivation experiment was conducted in 5 sq. m outdoor raceway tanks. However, for the outdoor experiment, carotenoid-rich biomass was harvested from the second phase by membrane filtration, and the growth media was recycled for cultivating in the first phase. The salinity of the culture continued to increase as the evaporated water was balanced by seawater. The semi-continuous cultivation of the first phase continued for 20 days before the culture salinity reached 7.4 % NaCl. For <em>Synechococcus</em> sp., the growth and carotenoid productivities were not affected within this salinity range.</p></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":null,"pages":null},"PeriodicalIF":4.6000,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Algal Research-Biomass Biofuels and Bioproducts","FirstCategoryId":"99","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2211926424002698","RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"BIOTECHNOLOGY & APPLIED MICROBIOLOGY","Score":null,"Total":0}
引用次数: 0
Abstract
The carotenoid productivity of a euryhaline Synechococcus sp. was studied in indoor and outdoor conditions. Indoor experiments revealed that the strain could maintain growth and carotenoid accumulation within a salinity range of 2.5–8.0 % NaCl. In an indoor two phase semi-continuous cultivation in PBRs, every three days, 50 % of the culture was replaced with fresh growth media in phase one, whereas the removed culture was grown for another three days in the second phase to enhance the carotenoid yield. Biomass and carotenoid productivity of the strain in the second phase were similar in five consecutive cultivation cycles. Next, the same two-phase cultivation experiment was conducted in 5 sq. m outdoor raceway tanks. However, for the outdoor experiment, carotenoid-rich biomass was harvested from the second phase by membrane filtration, and the growth media was recycled for cultivating in the first phase. The salinity of the culture continued to increase as the evaporated water was balanced by seawater. The semi-continuous cultivation of the first phase continued for 20 days before the culture salinity reached 7.4 % NaCl. For Synechococcus sp., the growth and carotenoid productivities were not affected within this salinity range.
期刊介绍:
Algal Research is an international phycology journal covering all areas of emerging technologies in algae biology, biomass production, cultivation, harvesting, extraction, bioproducts, biorefinery, engineering, and econometrics. Algae is defined to include cyanobacteria, microalgae, and protists and symbionts of interest in biotechnology. The journal publishes original research and reviews for the following scope: algal biology, including but not exclusive to: phylogeny, biodiversity, molecular traits, metabolic regulation, and genetic engineering, algal cultivation, e.g. phototrophic systems, heterotrophic systems, and mixotrophic systems, algal harvesting and extraction systems, biotechnology to convert algal biomass and components into biofuels and bioproducts, e.g., nutraceuticals, pharmaceuticals, animal feed, plastics, etc. algal products and their economic assessment