Algal Research-Biomass Biofuels and Bioproducts最新文献

英文中文

Differential responses of Microcystis aeruginosa to single and repeated exposure to Myriophyllum spicatum extracts 铜绿微囊藻对单一和重复暴露于叶绿素提取物的不同反应

IF 4.6 2区生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Algal Research-Biomass Biofuels and Bioproducts

Pub Date : 2025-04-23 DOI: 10.1016/j.algal.2025.104060

Qing Cao, Bensheng You, Haibo Xu, Weijing Liu, Shuzhan Ma

Allelochemicals have been proven to be effective in inhibiting microalgae. However, there is limited knowledge regarding their effect on cyanobacteria in the case of repeated exposure, which is more environmentally relevant. In the present study, the effects of multiple low-dosage additions and single high-dosage addition of Myriophyllum spicatum extracts toward Microcystis aeruginosa were compared based on cell density, photosynthetic response, antioxidative response, and microcystins (MCs) production. Although the cumulative exposure content of M. spicatum extracts was the same (5 g/L), our results showed that algal growth was promoted in repeated low-dosage exposure, whereas single high-dosage exposure inhibited cell growth and decreased photosynthetic activity with the highest inhibition rate of cell density reaching 58.2 %. Oxidative stress occurred in all treatments exposed to M. spicatum extracts, as indicated by the increased catalase (CAT) activity and superoxide Dismutase (SOD) activity. It is noteworthy that oxidative damage to the cell membrane only occurred in single high-dosage exposure as indicated by the malondialdehyde (MDA) contents, which were 1.9, 2.1, 2.8, and 2.7 times higher than that of the control group from day 2 to day 5, respectively. Moreover, repeated low-dosage exposure could promote MC synthesis as indicated by the up-regulated mcyD gene abundance. Cell lysis may be responsible for the increased MCs release in single high-dosage exposure treatment since active transport of MCs was suppressed as indicated by mcyH gene abundance. This study revealed differential responses of M. aeruginosa to single and repeated exposure to allelochemicals, shedding light on the allopathy control of cyanobacteria.

{"title":"Differential responses of Microcystis aeruginosa to single and repeated exposure to Myriophyllum spicatum extracts","authors":"Qing Cao, Bensheng You, Haibo Xu, Weijing Liu, Shuzhan Ma","doi":"10.1016/j.algal.2025.104060","DOIUrl":"10.1016/j.algal.2025.104060","url":null,"abstract":"<div><div>Allelochemicals have been proven to be effective in inhibiting microalgae. However, there is limited knowledge regarding their effect on cyanobacteria in the case of repeated exposure, which is more environmentally relevant. In the present study, the effects of multiple low-dosage additions and single high-dosage addition of <em>Myriophyllum spicatum</em> extracts toward <em>Microcystis aeruginosa</em> were compared based on cell density, photosynthetic response, antioxidative response, and microcystins (MCs) production. Although the cumulative exposure content of <em>M. spicatum</em> extracts was the same (5 g/L), our results showed that algal growth was promoted in repeated low-dosage exposure, whereas single high-dosage exposure inhibited cell growth and decreased photosynthetic activity with the highest inhibition rate of cell density reaching 58.2 %. Oxidative stress occurred in all treatments exposed to <em>M. spicatum</em> extracts, as indicated by the increased catalase (CAT) activity and superoxide Dismutase (SOD) activity. It is noteworthy that oxidative damage to the cell membrane only occurred in single high-dosage exposure as indicated by the malondialdehyde (MDA) contents, which were 1.9, 2.1, 2.8, and 2.7 times higher than that of the control group from day 2 to day 5, respectively. Moreover, repeated low-dosage exposure could promote MC synthesis as indicated by the up-regulated <em>mcyD</em> gene abundance. Cell lysis may be responsible for the increased MCs release in single high-dosage exposure treatment since active transport of MCs was suppressed as indicated by <em>mcyH</em> gene abundance. This study revealed differential responses of <em>M. aeruginosa</em> to single and repeated exposure to allelochemicals, shedding light on the allopathy control of cyanobacteria.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"89 ","pages":"Article 104060"},"PeriodicalIF":4.6,"publicationDate":"2025-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143864730","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enhancing phycocyanin productivity and thermostability in Synechocystis sp. AUPL1 using Ulva lactuca hydrolysates and ulvan polysaccharides

IF 4.6 2区生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Algal Research-Biomass Biofuels and Bioproducts

Pub Date : 2025-04-19 DOI: 10.1016/j.algal.2025.104053

Mohamed Gomaa, Shimaa Abdelmohsen Ali, Awatief F. Hifney

Increasing the productivity and thermostability of phycocyanin (PC) are key challenges in its industrial production. This study utilized aqueous and acidic hydrolysates derived from the seaweed Ulva lactuca for fed-batch mixotrophic cultivation of Synechocystis sp. AUPL1. The acidic hydrolysate contained higher levels of nutrients and reducing sugars (0.08 mg mL⁻¹) compared to the aqueous hydrolysate (0.03 mg mL⁻¹). Supplementing the cyanobacterial culture with 3 % (v/v) acidic hydrolysate every three days significantly increased biomass productivity, PC content, and PC productivity, achieving approximately 2-, 3-, and 6.5-fold improvements, respectively, compared to the autotrophic control. Additionally, ulvan polysaccharide, extracted from the same seaweed, was tested as a natural preservative to prevent PC degradation at elevated temperatures. Ulvan at 3 % (w/v) significantly extended the PC half-life at 60 °C to 293.21 min, compared to 101.65 min in the control. Thermodynamic analysis confirmed the thermostabilizing effect of the PC-ulvan mixture, revealing that the thermal degradation process was endothermic and non-spontaneous. This study underscores the potential of U. lactuca biomass as a low-cost, sustainable nutrient source for enhancing PC productivity, while also demonstrating that ulvan effectively improves PC thermostability.

{"title":"Enhancing phycocyanin productivity and thermostability in Synechocystis sp. AUPL1 using Ulva lactuca hydrolysates and ulvan polysaccharides","authors":"Mohamed Gomaa, Shimaa Abdelmohsen Ali, Awatief F. Hifney","doi":"10.1016/j.algal.2025.104053","DOIUrl":"10.1016/j.algal.2025.104053","url":null,"abstract":"<div><div>Increasing the productivity and thermostability of phycocyanin (PC) are key challenges in its industrial production. This study utilized aqueous and acidic hydrolysates derived from the seaweed <em>Ulva lactuca</em> for fed-batch mixotrophic cultivation of <em>Synechocystis</em> sp. AUPL1. The acidic hydrolysate contained higher levels of nutrients and reducing sugars (0.08 mg mL<sup>−1</sup>) compared to the aqueous hydrolysate (0.03 mg mL<sup>−1</sup>). Supplementing the cyanobacterial culture with 3 % (v/v) acidic hydrolysate every three days significantly increased biomass productivity, PC content, and PC productivity, achieving approximately 2-, 3-, and 6.5-fold improvements, respectively, compared to the autotrophic control. Additionally, ulvan polysaccharide, extracted from the same seaweed, was tested as a natural preservative to prevent PC degradation at elevated temperatures. Ulvan at 3 % (w/v) significantly extended the PC half-life at 60 °C to 293.21 min, compared to 101.65 min in the control. Thermodynamic analysis confirmed the thermostabilizing effect of the PC-ulvan mixture, revealing that the thermal degradation process was endothermic and non-spontaneous. This study underscores the potential of <em>U. lactuca</em> biomass as a low-cost, sustainable nutrient source for enhancing PC productivity, while also demonstrating that ulvan effectively improves PC thermostability.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"89 ","pages":"Article 104053"},"PeriodicalIF":4.6,"publicationDate":"2025-04-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143860401","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Low-cost cocultivation of Vischeria stellata and Tribonema sp. biomass using molasses vinasse: A novel wastewater reuse model for functional aquatic feed production

IF 4.6 2区生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Algal Research-Biomass Biofuels and Bioproducts

Pub Date : 2025-04-18 DOI: 10.1016/j.algal.2025.104051

Yuexin Sun , Yu Jiang , Jinghua Pang, Yuxuan Zhang, Yuying Deng, Tianche Zhao, Peihong Shen, Luodong Huang

Microalgae can effectively sequester CO₂, cultivating dense algal populations and producing high-value biomass in acidic environments poses significant challenges. This study explored the growth of unicellular Vischeria stellata GXU-A13 and filamentous Tribonema sp. GXU-A10 in acid molasses vinasse (MV). Results revealed that GXU-A13 exhibited strong acid tolerance, with maximum biomass reaching 2.71 g/L in MV wastewater (pH 5) and a COD removal rate of 49.4 % after 12 days. Notably, a mixed culture of the easily harvested GXU-A10 and acid-resistant GXU-A13 demonstrated enhanced wastewater treatment, with COD removal rates of 64.3 % and NH₄-N removal rates of 54.9 %. Additionally, the GXU-10 and GXU-A13 co-culture also achieved efficient biomass recovery, and examined the effects of this biomass as feed on the growth and immune function of Oreochromis niloticus. Feeding O. niloticus with 2.5 % or 5 % GXU-A13 (V2 and V5 group) resulted in significant improvements in specific growth rate (SGR). Additionally, GXU-A13 significantly enhanced glutathione peroxidase (GSH-PX) activity and decreased malondialdehyde (MDA) levels, which contributed to a marked increase in intestinal microbial diversity. Furthermore, diets containing 2.5 % GXU-A13 (V2 group) and 2.5 % GXU-A10 (H2 group) exhibited stronger antioxidant capacity and immune response. The dual metabolic benefits of mixed algae (VH group) that contained 2.5 % GXU-A13 and 2.5 % GXU-A10 significantly improved intestinal and liver health, while enhancing O. niloticus tolerance to high-ammonia nitrogen wastewater (NH₄⁺ concentration of 114 ± 3.2 mg/L). This study effectively integrates the unique characteristics of the two microalgae, enabling MV purification and recycling, advancing the development of a microalgae-based circular economy, and enhancing the quality and sustainability of aquatic feed.

{"title":"Low-cost cocultivation of Vischeria stellata and Tribonema sp. biomass using molasses vinasse: A novel wastewater reuse model for functional aquatic feed production","authors":"Yuexin Sun , Yu Jiang , Jinghua Pang, Yuxuan Zhang, Yuying Deng, Tianche Zhao, Peihong Shen, Luodong Huang","doi":"10.1016/j.algal.2025.104051","DOIUrl":"10.1016/j.algal.2025.104051","url":null,"abstract":"<div><div>Microalgae can effectively sequester CO<sub>2</sub>, cultivating dense algal populations and producing high-value biomass in acidic environments poses significant challenges. This study explored the growth of unicellular <em>Vischeria stellata</em> GXU-A13 and filamentous <em>Tribonema</em> sp. GXU-A10 in acid molasses vinasse (MV). Results revealed that GXU-A13 exhibited strong acid tolerance, with maximum biomass reaching 2.71 g/L in MV wastewater (pH 5) and a COD removal rate of 49.4 % after 12 days. Notably, a mixed culture of the easily harvested GXU-A10 and acid-resistant GXU-A13 demonstrated enhanced wastewater treatment, with COD removal rates of 64.3 % and NH<sub>4</sub>-N removal rates of 54.9 %. Additionally, the GXU-10 and GXU-A13 co-culture also achieved efficient biomass recovery, and examined the effects of this biomass as feed on the growth and immune function of <em>Oreochromis niloticus</em>. Feeding <em>O. niloticus</em> with 2.5 % or 5 % GXU-A13 (V2 and V5 group) resulted in significant improvements in specific growth rate (SGR). Additionally, GXU-A13 significantly enhanced glutathione peroxidase (GSH-PX) activity and decreased malondialdehyde (MDA) levels, which contributed to a marked increase in intestinal microbial diversity. Furthermore, diets containing 2.5 % GXU-A13 (V2 group) and 2.5 % GXU-A10 (H2 group) exhibited stronger antioxidant capacity and immune response. The dual metabolic benefits of mixed algae (VH group) that contained 2.5 % GXU-A13 and 2.5 % GXU-A10 significantly improved intestinal and liver health, while enhancing <em>O. niloticus</em> tolerance to high-ammonia nitrogen wastewater (NH<sub>4</sub><sup>+</sup> concentration of 114 ± 3.2 mg/L). This study effectively integrates the unique characteristics of the two microalgae, enabling MV purification and recycling, advancing the development of a microalgae-based circular economy, and enhancing the quality and sustainability of aquatic feed.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"89 ","pages":"Article 104051"},"PeriodicalIF":4.6,"publicationDate":"2025-04-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143860404","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Predicting chemical toxicity towards Raphidocelis subcapitata with quantum chemical descriptors

IF 4.6 2区生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Algal Research-Biomass Biofuels and Bioproducts

Pub Date : 2025-04-18 DOI: 10.1016/j.algal.2025.104055

Xinliang Yu

The green alga Raphidocelis subcapitata is an important model organism in toxicity studies. This paper, for the first time, reported classification models for Raphidocelis subcapitata toxicities (pEC₅₀ and pEC₁₀), by applying 43 quantum chemical descriptors and the random forest algorithm. Both classification models for the toxicities pEC₅₀ and pEC₁₀ have prediction accuracy values of 100 % for the training set (251 organics) and above 90 % for the test set (83 organics). The quantum chemical descriptors selected include atomic charges, multipole moments, frontier orbital energies and kinetic energies, polarizability and thermochemistry properties. They are related to reaction sites, electrophilic and nucleophilic reactivity, chemical bond formation, hydrogen bonds and electrostatic force. The two classification models based on a sufficiently large sample set provide an important tool for assessing the toxicity categories of organics towards Raphidocelis subcapitata.

引用次数: 0

Enhancing lutein concentration in an indigenous microalgal strain through salinity, light intensity and nutrient concentrations and evaluation of its anticancer potential

IF 4.6 2区生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Algal Research-Biomass Biofuels and Bioproducts

Pub Date : 2025-04-17 DOI: 10.1016/j.algal.2025.104054

Arima Marchese , Serena Lima , Valeria Villanova , Eleonora Montuori , Daniele De Luca , Chiara Lauritano , Francesca Scargiali

The search for new approaches for treating cancer is an urgent need and microalgae have recently shown to be an underestimated source of biocompounds. In this work, the anticancer potential of an indigenous Chlorella-like microalgal strain was investigated by optimizing its lutein content using a Design of Experiment (DoE) approach. Several parameters were assessed such as NaCl, phosphate and nitrate concentration in combination with light intensity. Sodium chloride and nitrate concentrations showed antagonist effects on lutein accumulation. The crude methanolic extracts of the obtained microalgal cultures, containing 2.15 ± 0.12, 1.70 ± 0.17 and of 0.68 ± 0.09 μg mg⁻¹ of lutein, were tested on cancer cell lines, revealing a dosage-dependent antiproliferative effect on melanoma A2058 cells. The extracts' fractions containing lutein exhibited similar effects, likely due to a concerted action of multiple substances, with lutein being one of the main contributors. The described approach, which combined bioprocess optimization and assessment of anticancer proprieties, showed a significant potential in the discovery of new bioactives for pharmaceutics.

{"title":"Enhancing lutein concentration in an indigenous microalgal strain through salinity, light intensity and nutrient concentrations and evaluation of its anticancer potential","authors":"Arima Marchese , Serena Lima , Valeria Villanova , Eleonora Montuori , Daniele De Luca , Chiara Lauritano , Francesca Scargiali","doi":"10.1016/j.algal.2025.104054","DOIUrl":"10.1016/j.algal.2025.104054","url":null,"abstract":"<div><div>The search for new approaches for treating cancer is an urgent need and microalgae have recently shown to be an underestimated source of biocompounds. In this work, the anticancer potential of an indigenous <em>Chlorella-</em>like microalgal strain was investigated by optimizing its lutein content using a Design of Experiment (DoE) approach. Several parameters were assessed such as NaCl, phosphate and nitrate concentration in combination with light intensity. Sodium chloride and nitrate concentrations showed antagonist effects on lutein accumulation. The crude methanolic extracts of the obtained microalgal cultures, containing 2.15 ± 0.12, 1.70 ± 0.17 and of 0.68 ± 0.09 μg mg<sup>−1</sup> of lutein, were tested on cancer cell lines, revealing a dosage-dependent antiproliferative effect on melanoma A2058 cells. The extracts' fractions containing lutein exhibited similar effects, likely due to a concerted action of multiple substances, with lutein being one of the main contributors. The described approach, which combined bioprocess optimization and assessment of anticancer proprieties, showed a significant potential in the discovery of new bioactives for pharmaceutics.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"89 ","pages":"Article 104054"},"PeriodicalIF":4.6,"publicationDate":"2025-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143864741","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluation of algal strains for carbohydrate production: Potentials of Chlorella lewinii FwC1 under different trophic modes and in open-pond conditions

IF 4.6 2区生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Algal Research-Biomass Biofuels and Bioproducts

Pub Date : 2025-04-17 DOI: 10.1016/j.algal.2025.104050

Chandrani Debnath , Tarun Kanti Bandyopadhyay , Muthusivaramapandian Muthuraj

Microalgae-based carbohydrates have been identified as a potential feedstock for third-generation biofuel production, but with several limitations from strain selection to mass production. Thus, identifying an appropriate microalgal cellular factory for mass-scale production of carbohydrates is essential. The present study screened eight algal strains with a multi-criteria approach, identifying Chlorella lewinii FwC1 as the most effective, with carbohydrate productivity of 19.76 mg L⁻¹ day⁻¹ and biomass yield of 0.15 g mol⁻¹ photon under photoautotrophic conditions. This strain thrived across various temperatures (24–36 °C) and pH (5.5–10.5). Under mixotrophic conditions with glucose, it achieved maximum biomass and carbohydrate productivity of 414 and 208 mg L⁻¹ day⁻¹, respectively, significantly surpassing heterotrophic and photoautotrophic growth. Sodium bicarbonate supplementation enhanced biomass productivity by ∼2-fold under photoautotrophic and 1.67-fold under mixotrophic conditions. Further, optimization of photoautotrophic condition improved biomass and carbohydrate productivity to 190 and 64.6 mg L⁻¹ day⁻¹, respectively, with a biomass yield of 0.38 g mol⁻¹ photon. Under open-pond conditions at a 300 L scale with optimal media composition, productivity reached 100.2 mg L⁻¹ day⁻¹ for biomass and 34.5 mg L⁻¹ day⁻¹ for carbohydrates. Thus, Chlorella lewinii FwC1 is a promising candidate for carbohydrate production.

{"title":"Evaluation of algal strains for carbohydrate production: Potentials of Chlorella lewinii FwC1 under different trophic modes and in open-pond conditions","authors":"Chandrani Debnath , Tarun Kanti Bandyopadhyay , Muthusivaramapandian Muthuraj","doi":"10.1016/j.algal.2025.104050","DOIUrl":"10.1016/j.algal.2025.104050","url":null,"abstract":"<div><div>Microalgae-based carbohydrates have been identified as a potential feedstock for third-generation biofuel production, but with several limitations from strain selection to mass production. Thus, identifying an appropriate microalgal cellular factory for mass-scale production of carbohydrates is essential. The present study screened eight algal strains with a multi-criteria approach, identifying <em>Chlorella lewinii</em> FwC1 as the most effective, with carbohydrate productivity of 19.76 mg L<sup>−1</sup> day<sup>−1</sup> and biomass yield of 0.15 g mol<sup>−1</sup> photon under photoautotrophic conditions. This strain thrived across various temperatures (24–36 °C) and pH (5.5–10.5). Under mixotrophic conditions with glucose, it achieved maximum biomass and carbohydrate productivity of 414 and 208 mg L<sup>−1</sup> day<sup>−1</sup>, respectively, significantly surpassing heterotrophic and photoautotrophic growth. Sodium bicarbonate supplementation enhanced biomass productivity by ∼2-fold under photoautotrophic and 1.67-fold under mixotrophic conditions. Further, optimization of photoautotrophic condition improved biomass and carbohydrate productivity to 190 and 64.6 mg L<sup>−1</sup> day<sup>−1</sup>, respectively, with a biomass yield of 0.38 g mol<sup>−1</sup> photon. Under open-pond conditions at a 300 L scale with optimal media composition, productivity reached 100.2 mg L<sup>−1</sup> day<sup>−1</sup> for biomass and 34.5 mg L<sup>−1</sup> day<sup>−1</sup> for carbohydrates. Thus, <em>Chlorella lewinii</em> FwC1 is a promising candidate for carbohydrate production.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"89 ","pages":"Article 104050"},"PeriodicalIF":4.6,"publicationDate":"2025-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143864728","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Compliance assessment oriented microcystin prediction: A Bayesian adaptive LASSO Tobit quantile regression approach

IF 4.6 2区生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Algal Research-Biomass Biofuels and Bioproducts

Pub Date : 2025-04-17 DOI: 10.1016/j.algal.2025.104026

Fan Qu , Lingjing Lin , Changbo Qin , Fuli Peng , Runzi Wang , Nengwang Chen , Gang Zhao , Wentao Lu , Zhongyao Liang

Microcystin has been one of major contaminants impacting health of aquatic ecosystems and threatening human health. The development of drivers-microcystin relationship is of vital importance to microcystin management. However, current practices often focused on the mean response of microcystin concentration and cannot meet the requirement of percentile-based compliance assessment. Despite of many informative studies on the development of drivers–microcystin relationship, there remains a gap between the relationship development and the percentile-based compliance assessment of microcystin concentration. In this study, Bayesian adaptive LASSO Tobit quantile regression (BALTQR) model was introduced to environmental and ecological studies for the first time. The model is specially designed for the prediction of left-censored response variable. We applied the BALTQR model to develop the drivers–microcystin relationship of lakes across the US continent. Based on the results of parameters estimation, Chlorophyll a (CHL), pH, and water temperature (WT) were identified as key drivers to the microcystin concentration. We found that CHL was approximate the same important as pH and both of them had positive effects on the microcystin concentration at all the five regression quantiles. WT was relatively less important and had a surprisingly negative effect at the 0.7, 0.8, and 0.9 regression quantiles. We demonstrated that the BALTQR model successfully established the linkage between the development of drivers–microcystin relationship and the compliance assessment of microcystin concentration. We further revealed important implications of these findings to microcystin management. We believed that the BALTQR model has great potential of generalization to model other left-censored response variable in environmental and ecological studies.

{"title":"Compliance assessment oriented microcystin prediction: A Bayesian adaptive LASSO Tobit quantile regression approach","authors":"Fan Qu , Lingjing Lin , Changbo Qin , Fuli Peng , Runzi Wang , Nengwang Chen , Gang Zhao , Wentao Lu , Zhongyao Liang","doi":"10.1016/j.algal.2025.104026","DOIUrl":"10.1016/j.algal.2025.104026","url":null,"abstract":"<div><div>Microcystin has been one of major contaminants impacting health of aquatic ecosystems and threatening human health. The development of drivers-microcystin relationship is of vital importance to microcystin management. However, current practices often focused on the mean response of microcystin concentration and cannot meet the requirement of percentile-based compliance assessment. Despite of many informative studies on the development of drivers–microcystin relationship, there remains a gap between the relationship development and the percentile-based compliance assessment of microcystin concentration. In this study, Bayesian adaptive LASSO Tobit quantile regression (BALTQR) model was introduced to environmental and ecological studies for the first time. The model is specially designed for the prediction of left-censored response variable. We applied the BALTQR model to develop the drivers–microcystin relationship of lakes across the US continent. Based on the results of parameters estimation, Chlorophyll <em>a</em> (CHL), pH, and water temperature (WT) were identified as key drivers to the microcystin concentration. We found that CHL was approximate the same important as pH and both of them had positive effects on the microcystin concentration at all the five regression quantiles. WT was relatively less important and had a surprisingly negative effect at the 0.7, 0.8, and 0.9 regression quantiles. We demonstrated that the BALTQR model successfully established the linkage between the development of drivers–microcystin relationship and the compliance assessment of microcystin concentration. We further revealed important implications of these findings to microcystin management. We believed that the BALTQR model has great potential of generalization to model other left-censored response variable in environmental and ecological studies.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"89 ","pages":"Article 104026"},"PeriodicalIF":4.6,"publicationDate":"2025-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143844290","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Microwave-assisted depolymerization of hydrothermally extracted carrageenan from Chondrus crispus

IF 4.6 2区生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Algal Research-Biomass Biofuels and Bioproducts

Pub Date : 2025-04-17 DOI: 10.1016/j.algal.2025.104046

Milena Álvarez-Viñas, María Dolores Torres, Herminia Domínguez

Carrageenans, extracted from red macroalgae, have demonstrated to present a wide variety of biological activities, such as antitumor activity, which could be enhanced upon depolymerization techniques. For that reason, this work dealt with the extraction of oligocarrageenans and carrageenan from Chondrus crispus by autohydrolysis, and the evaluation of microwave-assisted depolymerization to evaluate the improvement of its antitumor activity. This activity may be conditioned by the extraction method, the degree of sulfation, or the degree of depolymerization. Hence, these parameters were assessed, revealing an increase on the soluble sulfate content and lower molecular weight after 25 min of processing at 140 or 160 °C. In addition, the extract viscosity was lowered from 10,000 to 100 Pa·s, suggesting a positive effect of the microwave-assisted depolymerization and an enhanced bioavailability. The antitumor activity of the depolymerized oligocarrageenan and carrageenan was studied against human ovarian carcinoma cell line, reaching up to 92 % of cell growth inhibition (IC₅₀ = 84 mg/L).

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引用次数: 0

Effects of Sargassum beds on carbon sequestration and microbial community structure in the adjacent sediment cores

IF 4.6 2区生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Algal Research-Biomass Biofuels and Bioproducts

Pub Date : 2025-04-17 DOI: 10.1016/j.algal.2025.104045

Lili Xu , Xian Sun , Zhiwei Liu , Ligong Zou , Zhili He , Juan Ling , Linjian Ou , Bin Jiang , Yufeng Yang , Qing Wang

Seaweed detritus transported to the deep sea is an important component of marine carbon sequestration. However, the impacts of seaweed decomposition on adjacent sediments and the response mechanisms of microbial communities are still unclear. In this study, we analyzed the changes in carbon and nitrogen contents, the molecular composition of dissolved organic matter (DOM), and microbial communities in three sediment cores at different distances (1, 2, and 3 km, respectively) from Sargassum beds in Weizhou Island coastal waters, China. The results show that the impact of Sargassum on sediment cores exhibits strong spatial heterogeneity, with more aromatic-like and tannin molecules found at the nearest site (S1). Compared to the other two sediment cores, the microbial community composition of S1 is significantly different. The homogeneous selection dominates the assembly of the microbial community at S1. In addition, the input of decomposing organic matter from Sargassum contributed to a significant increase in the abundance of functional genes related to carbon and nitrogen decomposition at S1, leading to a significant decrease in TOC and TN content in the sediments. Our study demonstrated that the decomposition of Sargassum increased the sources and molecular species of organic matter in the adjacent sediments and stimulated microbial decomposition of organic matter. Strengthening the protection of Sargassum beds contributes to improving biodiversity and promoting the marine carbon and nitrogen cycles.

{"title":"Effects of Sargassum beds on carbon sequestration and microbial community structure in the adjacent sediment cores","authors":"Lili Xu , Xian Sun , Zhiwei Liu , Ligong Zou , Zhili He , Juan Ling , Linjian Ou , Bin Jiang , Yufeng Yang , Qing Wang","doi":"10.1016/j.algal.2025.104045","DOIUrl":"10.1016/j.algal.2025.104045","url":null,"abstract":"<div><div>Seaweed detritus transported to the deep sea is an important component of marine carbon sequestration. However, the impacts of seaweed decomposition on adjacent sediments and the response mechanisms of microbial communities are still unclear. In this study, we analyzed the changes in carbon and nitrogen contents, the molecular composition of dissolved organic matter (DOM), and microbial communities in three sediment cores at different distances (1, 2, and 3 km, respectively) from <em>Sargassum</em> beds in Weizhou Island coastal waters, China. The results show that the impact of <em>Sargassum</em> on sediment cores exhibits strong spatial heterogeneity, with more aromatic-like and tannin molecules found at the nearest site (S1). Compared to the other two sediment cores, the microbial community composition of S1 is significantly different. The homogeneous selection dominates the assembly of the microbial community at S1. In addition, the input of decomposing organic matter from <em>Sargassum</em> contributed to a significant increase in the abundance of functional genes related to carbon and nitrogen decomposition at S1, leading to a significant decrease in TOC and TN content in the sediments. Our study demonstrated that the decomposition of <em>Sargassum</em> increased the sources and molecular species of organic matter in the adjacent sediments and stimulated microbial decomposition of organic matter. Strengthening the protection of <em>Sargassum</em> beds contributes to improving biodiversity and promoting the marine carbon and nitrogen cycles.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"89 ","pages":"Article 104045"},"PeriodicalIF":4.6,"publicationDate":"2025-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143855195","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Collection of rich microalgae from textile wastewater and utilized for hydrogen production via hydrothermal gasification route: Performance study

IF 4.6 2区生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Algal Research-Biomass Biofuels and Bioproducts

Pub Date : 2025-04-17 DOI: 10.1016/j.algal.2025.104052

Manzoore Elahi M. Soudagar , Aman Sharma , R. Srinivasan , Nagabhooshanam Nagarajan , Vinayagam Mohanavel , M. Ravichandran , Manikandan Ayyar , Sami Al Obaid , Sulaiman Ali Alharbi

In this investigation, textile wastewater was treated in an open raceway pond to promote algae growth for biomass production. Various environmental factors initially hindered the growth of algae. However, the growth rate was improved by integrating titanium dioxide (TiO₂) nanoparticles at different concentrations. The concentrations tested included 0, 2, 4, and 6 % by weight in the wastewater. The study analyzed specific growth rates and biomass production resulting from these variations. Additionally, the cultivated algae were utilized in hydrothermal gasification for hydrogen production. This gasification process was conducted under a residence time of 30 min and a pressure of 200 bar, with gasification temperatures of 350, 450, 550, and 650 °C. Gasification efficiency was further enhanced using a potassium hydroxide (KOH) catalyst. At a higher TiO₂ nanoparticle concentration of 6 %, the specific growth rate and biomass production achieved were approximately 67.9 % and 75.3 %, respectively. In the hydrothermal gasification process, the gasification efficiency (GE), hydrogen selectivity, and higher heating value (HHV) were measured at about 81.5 %, 94.6 %, and 31.5 MJ/Nm³ at 650 °C with the 5 % of KOH catalyst, showing a significant improvement compared to conditions without a catalyst.

{"title":"Collection of rich microalgae from textile wastewater and utilized for hydrogen production via hydrothermal gasification route: Performance study","authors":"Manzoore Elahi M. Soudagar , Aman Sharma , R. Srinivasan , Nagabhooshanam Nagarajan , Vinayagam Mohanavel , M. Ravichandran , Manikandan Ayyar , Sami Al Obaid , Sulaiman Ali Alharbi","doi":"10.1016/j.algal.2025.104052","DOIUrl":"10.1016/j.algal.2025.104052","url":null,"abstract":"<div><div>In this investigation, textile wastewater was treated in an open raceway pond to promote algae growth for biomass production. Various environmental factors initially hindered the growth of algae. However, the growth rate was improved by integrating titanium dioxide (TiO<sub>2</sub>) nanoparticles at different concentrations. The concentrations tested included 0, 2, 4, and 6 % by weight in the wastewater. The study analyzed specific growth rates and biomass production resulting from these variations. Additionally, the cultivated algae were utilized in hydrothermal gasification for hydrogen production. This gasification process was conducted under a residence time of 30 min and a pressure of 200 bar, with gasification temperatures of 350, 450, 550, and 650 °C. Gasification efficiency was further enhanced using a potassium hydroxide (KOH) catalyst. At a higher TiO<sub>2</sub> nanoparticle concentration of 6 %, the specific growth rate and biomass production achieved were approximately 67.9 % and 75.3 %, respectively. In the hydrothermal gasification process, the gasification efficiency (GE), hydrogen selectivity, and higher heating value (HHV) were measured at about 81.5 %, 94.6 %, and 31.5 MJ/Nm<sup>3</sup> at 650 °C with the 5 % of KOH catalyst, showing a significant improvement compared to conditions without a catalyst.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"89 ","pages":"Article 104052"},"PeriodicalIF":4.6,"publicationDate":"2025-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143860454","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

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