Investigation of guanidino acetic acid and rumen-protected methionine induced improvements in longissimus lumborum muscle quality in beef cattle

IF 7.1 1区 农林科学 Q1 Agricultural and Biological Sciences Meat Science Pub Date : 2024-08-05 DOI:10.1016/j.meatsci.2024.109624
Simeng Yi , Boping Ye , Jinze Wang , Xin Yi , Yao Wang , Abudusaimijiang Abudukelimu , Hao Wu , Qingxiang Meng , Zhenming Zhou
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Abstract

This study examined the impact of dietary guanidino acetic acid (GAA) and rumen-protected methionine (RPM) on beef quality in Simmental bulls. For 140 days, forty-five bulls (453.43 ± 29.05 kg) were randomly divided into control (CON), 0.1% GAA (GAA), and 0.1% GAA + 0.1% RPM (GAM) groups with 15 bulls in each group and containing 3 pen with 5 bulls in each pen. Significant improvements in eye muscle area, pH48h, redness (a*) value, and crude protein (CP) content of longissimus lumborum (LL) muscles were observed in the GAA and GAM groups (P < 0.05). Conversely, the lightness (L*) value, drip loss, cooking loss, and moisture contents decreased (P < 0.05). Additionally, glutathione (GSH) and glutathione peroxidase (GSH-PX) concentrations of LL muscles in GAM were higher (P < 0.05), while malondialdehyde (MDA) content of LL muscles in GAA and GAM groups were lower (P < 0.05). Polyunsaturated fatty acids (PUFA) profiles were enriched in beef from GAM group (P < 0.05). The addition of GAA and RPM affected the expression of genes in LL muscle, such as HMOX1, EIF4E, SCD5, and NOS2, which are related to hypoxia metabolism, protein synthesis, and unsaturated fatty acid synthesis-related signaling pathways. In addition, GAA and RPM also affected the content of a series of metabolites such as L-tyrosine, L-tryptophan, and PC (O-16:0/0:0) involved in amino acid and lipid metabolism-related signaling pathways. In summary, GAA and RPM can improve the beef quality and its nutritional composition. These changes may be related to changes in gene expression and metabolic pathways related to protein metabolism and lipid metabolism in beef.

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胍基乙酸和瘤胃保护蛋氨酸改善肉牛腰长肌质量的研究
本研究考察了日粮胍基乙酸(GAA)和瘤胃保护蛋氨酸(RPM)对西门塔尔公牛牛肉品质的影响。将 45 头公牛(453.43 ± 29.05 千克)随机分为对照组(CON)、0.1% GAA 组(GAA)和 0.1% GAA + 0.1% RPM 组(GAM),每组 15 头公牛,共 3 个栏,每个栏 5 头公牛,饲养 140 天。GAA 组和 GAM 组的眼肌面积、pH48h 值、红度 (a*) 值和腰长肌 (LL) 的粗蛋白 (CP) 含量均有显著改善(P < 0.05)。相反,轻度(L*)值、滴水损失、蒸煮损失和水分含量则有所下降(P < 0.05)。此外,GAM 组 LL 肌肉的谷胱甘肽(GSH)和谷胱甘肽过氧化物酶(GSH-PX)浓度较高(P < 0.05),而 GAA 组和 GAM 组 LL 肌肉的丙二醛(MDA)含量较低(P < 0.05)。多不饱和脂肪酸(PUFA)在 GAM 组牛肉中富集(P < 0.05)。添加 GAA 和 RPM 会影响 LL 肌肉中 HMOX1、EIF4E、SCD5 和 NOS2 等基因的表达,这些基因与缺氧代谢、蛋白质合成和不饱和脂肪酸合成相关的信号通路有关。此外,GAA 和 RPM 还影响一系列代谢物的含量,如 L-酪氨酸、L-色氨酸和 PC(O-16:0/0:0),这些代谢物参与氨基酸和脂质代谢相关的信号通路。总之,GAA 和 RPM 可改善牛肉质量及其营养成分。这些变化可能与牛肉中与蛋白质代谢和脂质代谢相关的基因表达和代谢途径的变化有关。
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来源期刊
Meat Science
Meat Science 工程技术-食品科技
CiteScore
12.60
自引率
9.90%
发文量
282
审稿时长
60 days
期刊介绍: The aim of Meat Science is to serve as a suitable platform for the dissemination of interdisciplinary and international knowledge on all factors influencing the properties of meat. While the journal primarily focuses on the flesh of mammals, contributions related to poultry will be considered if they enhance the overall understanding of the relationship between muscle nature and meat quality post mortem. Additionally, papers on large birds (e.g., emus, ostriches) as well as wild-captured mammals and crocodiles will be welcomed.
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