Genomic characterization of methicillin-resistant Staphylococcus aureus isolated from patients attending regional referral hospitals in Tanzania.

IF 2.1 4区 医学 Q3 GENETICS & HEREDITY BMC Medical Genomics Pub Date : 2024-08-14 DOI:10.1186/s12920-024-01979-4
Mujungu A Geofrey, Elingarami Sauli, Livin E Kanje, Melkiory Beti, Mariana J Shayo, Davis Kuchaka, Marco van Zwetselaar, Boaz Wadugu, Blandina Mmbaga, Sixbert Isdory Mkumbaye, Happiness Kumburu, Tolbert Sonda
{"title":"Genomic characterization of methicillin-resistant Staphylococcus aureus isolated from patients attending regional referral hospitals in Tanzania.","authors":"Mujungu A Geofrey, Elingarami Sauli, Livin E Kanje, Melkiory Beti, Mariana J Shayo, Davis Kuchaka, Marco van Zwetselaar, Boaz Wadugu, Blandina Mmbaga, Sixbert Isdory Mkumbaye, Happiness Kumburu, Tolbert Sonda","doi":"10.1186/s12920-024-01979-4","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong>Methicillin-resistant Staphylococcus aureus (MRSA) colonization increases the risk of subsequent infection by MRSA strain complex interlinking between hospital and community-acquired MRSA which increases the chance of drug resistance and severity of the disease.</p><p><strong>Objective: </strong>Genomic characterization of Staphylococcus aures strains isolated from patients attending regional referral hospitals in Tanzania.</p><p><strong>Methodology: </strong>A laboratory-based cross-sectional study using short read-based sequencing technology, (Nextseq550,Illumina, Inc. San diego, California, USA). The samples used were collected from patients attending selected regional referral hospitals in Tanzania under the SeqAfrica project. Sequences were analyzed using tools available in the center for genomic and epidemiology server, and visualization of the phylogenetic tree was performed in ITOL 6.0. SPSS 28.0 was used for statistical analysis.</p><p><strong>Results: </strong>Among 103 sequences of S. aureus, 48.5% (50/103) carry the mecA gene for MRSA. High proportions of MRSA were observed among participants aged between 18 and 34 years (52.4%), in females (54.3%), and among outpatients (60.5%). The majority of observed MRSA carried plasmids rep5a (92.0%), rep16 (90.0%), rep7c (90.0%), rep15 (82.0%), rep19 (80.0%) and rep10 (72.0%). Among all plasmids observed rep5a, rep16, rep20, and repUS70 carried the blaZ gene, rep10 carried the erm(C) gene and rep7a carried the tet(K) gene. MLST and phylogeny analysis reveal high diversity among MRSA. Six different clones were observed circulating at selected regional hospitals and MRSA with ST8 was dominant.</p><p><strong>Conclusion: </strong>The study reveals a significant presence of MRSA in Staphylococcus aureus strains from Tanzanian regional hospitals, with nearly half carrying the mecA gene. MRSA is notably prevalent among young adults, females, and outpatients, showing high genetic diversity and dominance of ST8. Various plasmids carrying resistance genes indicate a complex resistance profile, highlighting the need for targeted interventions to manage MRSA infections in Tanzania.</p>","PeriodicalId":8915,"journal":{"name":"BMC Medical Genomics","volume":"17 1","pages":"211"},"PeriodicalIF":2.1000,"publicationDate":"2024-08-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11323609/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"BMC Medical Genomics","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1186/s12920-024-01979-4","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"GENETICS & HEREDITY","Score":null,"Total":0}
引用次数: 0

Abstract

Background: Methicillin-resistant Staphylococcus aureus (MRSA) colonization increases the risk of subsequent infection by MRSA strain complex interlinking between hospital and community-acquired MRSA which increases the chance of drug resistance and severity of the disease.

Objective: Genomic characterization of Staphylococcus aures strains isolated from patients attending regional referral hospitals in Tanzania.

Methodology: A laboratory-based cross-sectional study using short read-based sequencing technology, (Nextseq550,Illumina, Inc. San diego, California, USA). The samples used were collected from patients attending selected regional referral hospitals in Tanzania under the SeqAfrica project. Sequences were analyzed using tools available in the center for genomic and epidemiology server, and visualization of the phylogenetic tree was performed in ITOL 6.0. SPSS 28.0 was used for statistical analysis.

Results: Among 103 sequences of S. aureus, 48.5% (50/103) carry the mecA gene for MRSA. High proportions of MRSA were observed among participants aged between 18 and 34 years (52.4%), in females (54.3%), and among outpatients (60.5%). The majority of observed MRSA carried plasmids rep5a (92.0%), rep16 (90.0%), rep7c (90.0%), rep15 (82.0%), rep19 (80.0%) and rep10 (72.0%). Among all plasmids observed rep5a, rep16, rep20, and repUS70 carried the blaZ gene, rep10 carried the erm(C) gene and rep7a carried the tet(K) gene. MLST and phylogeny analysis reveal high diversity among MRSA. Six different clones were observed circulating at selected regional hospitals and MRSA with ST8 was dominant.

Conclusion: The study reveals a significant presence of MRSA in Staphylococcus aureus strains from Tanzanian regional hospitals, with nearly half carrying the mecA gene. MRSA is notably prevalent among young adults, females, and outpatients, showing high genetic diversity and dominance of ST8. Various plasmids carrying resistance genes indicate a complex resistance profile, highlighting the need for targeted interventions to manage MRSA infections in Tanzania.

查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
从坦桑尼亚地区转诊医院病人中分离出的耐甲氧西林金黄色葡萄球菌的基因组特征。
背景:耐甲氧西林金黄色葡萄球菌(MRSA)定植增加了随后感染MRSA菌株的风险,医院和社区获得的MRSA菌株之间相互关联,增加了耐药性和疾病严重性的机会:从坦桑尼亚地区转诊医院的患者身上分离出的金黄色葡萄球菌菌株的基因组特征:一项基于实验室的横断面研究,采用基于短读的测序技术(Nextseq550,Illumina 公司,美国加利福尼亚州圣迭戈)。所使用的样本收集自坦桑尼亚 SeqAfrica 项目选定的地区转诊医院的患者。使用基因组和流行病学服务器中心提供的工具对序列进行分析,并使用 ITOL 6.0 对系统发生树进行可视化。统计分析采用 SPSS 28.0:在 103 个金黄色葡萄球菌序列中,48.5%(50/103)携带 MRSA 的 mecA 基因。在 18 至 34 岁的参与者(52.4%)、女性(54.3%)和门诊病人(60.5%)中,观察到的 MRSA 比例较高。观察到的大多数 MRSA 都携带质粒 rep5a(92.0%)、rep16(90.0%)、rep7c(90.0%)、rep15(82.0%)、rep19(80.0%)和 rep10(72.0%)。在观察到的所有质粒中,rep5a、rep16、rep20 和 repUS70 带有 blaZ 基因,rep10 带有 erm(C) 基因,rep7a 带有 tet(K) 基因。MLST 和系统进化分析表明 MRSA 具有高度的多样性。在选定的地区医院中观察到六种不同的克隆循环,其中 ST8 的 MRSA 占主导地位:研究显示,坦桑尼亚地区医院的金黄色葡萄球菌菌株中存在大量 MRSA,其中近一半携带 mecA 基因。MRSA在青壮年、女性和门诊病人中明显多发,显示出高度的遗传多样性和ST8的优势。携带抗药性基因的各种质粒显示出复杂的抗药性特征,突出表明需要采取有针对性的干预措施来控制坦桑尼亚的 MRSA 感染。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 去求助
来源期刊
BMC Medical Genomics
BMC Medical Genomics 医学-遗传学
CiteScore
3.90
自引率
0.00%
发文量
243
审稿时长
3.5 months
期刊介绍: BMC Medical Genomics is an open access journal publishing original peer-reviewed research articles in all aspects of functional genomics, genome structure, genome-scale population genetics, epigenomics, proteomics, systems analysis, and pharmacogenomics in relation to human health and disease.
期刊最新文献
Construction of an immunogenic cell death-related LncRNA signature to predict the prognosis of patients with lung adenocarcinoma. Protein-losing enteropathy with congenital kidney stones in a 2-month-old boy: a rare case report and literature review. Concomitant telomere attrition is associated with spinal muscular atrophy in highly inbred region of North India: unraveling the thread in Kashmir region. Development of a clinical metagenomics workflow for the diagnosis of wound infections. Genomic profiling and expanded use of targeted anticancer drugs in solid cancers with exhausted evidence-based treatment options (PRECODE): study protocol of a prospective, non-randomized, cohort study.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1