Enhancement of ε-poly-L-lysine production by Streptomyces albulus FQF-24 with feeding strategies using cassava starch as carbon source.

IF 3.5 3区 生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Bioprocess and Biosystems Engineering Pub Date : 2024-12-01 Epub Date: 2024-08-16 DOI:10.1007/s00449-024-03078-1
Boyan Li, Chenqi Wu, Senmeng Bai, Di Zhang, Chang Xu, Xiaofeng Yuan, Jiayi Tian, Jing Bai, Liangzhi Li, Jiaolong Fu
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Abstract

ε-Poly-L-lysine (ε-PL) is a natural and wide-spectrum antimicrobial additive. In this study, the production of ε-PL by Streptomyces albulus FQF-24 using cassava starch (CS) as carbon source and the effects of different feeding methods were investigated in a fermenter. The initial shake flask experiments demonstrated the efficient production of ε-PL with CS, achieving the ε-PL production of 1.18 g/L. Subsequent investigations in the fermenter identified that the ideal pH was 3.8 during the ε-PL synthesis phase. Under this condition, the production of ε-PL reached 1.35 g/L. When the pH was maintained at 3.8, the investigation of improvement of feeding composition was carried out in a 5 L fermenter. The intermittent feeding containing CS, inorganic and organic nitrogen sources resulted in the maximum ε-PL production and dry cell weight (DCW) reaching 17.17 g/L and 42.73 g/L. Additionally, continuous feeding with the composition of CS, organic and inorganic nitrogen sources, and inorganic salts further increased ε-PL production and DCW to 27.56 g/L and 38.5 g/L. Summarily, the above results indicate that the fermentation using low-cost CS and continuous feeding strategy with whole medium composition can provide a beneficial reference for the efficient production of ε-PL.

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以木薯淀粉为碳源的喂养策略提高白链霉菌 FQF-24 的ε-聚-L-赖氨酸产量
ε-聚-L-赖氨酸(ε-PL)是一种天然、广谱的抗菌添加剂。本研究以木薯淀粉(CS)为碳源,在发酵罐中研究了白链霉菌 FQF-24 生产ε-PL 的情况以及不同喂料方法的影响。最初的摇瓶实验表明,使用 CS 能高效生产ε-PL,ε-PL 产量达到 1.18 g/L。随后在发酵罐中进行的研究发现,ε-PL 合成阶段的理想 pH 值为 3.8。在此条件下,ε-PL 的产量达到了 1.35 克/升。当 pH 值保持在 3.8 时,在 5 升发酵罐中进行了改进投料成分的研究。含有 CS、无机氮和有机氮源的间歇喂料使ε-PL 产量和干细胞重量(DCW)达到最大值,分别为 17.17 克/升和 42.73 克/升。此外,连续饲喂含有 CS、有机和无机氮源以及无机盐成分的饲料可进一步提高ε-PL 产量和干细胞重量(DCW),分别达到 27.56 克/升和 38.5 克/升。总之,上述结果表明,采用低成本 CS 发酵和全培养基成分的连续饲喂策略可为高效生产ε-PL 提供有益的参考。
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来源期刊
Bioprocess and Biosystems Engineering
Bioprocess and Biosystems Engineering 工程技术-工程:化工
CiteScore
7.90
自引率
2.60%
发文量
147
审稿时长
2.6 months
期刊介绍: Bioprocess and Biosystems Engineering provides an international peer-reviewed forum to facilitate the discussion between engineering and biological science to find efficient solutions in the development and improvement of bioprocesses. The aim of the journal is to focus more attention on the multidisciplinary approaches for integrative bioprocess design. Of special interest are the rational manipulation of biosystems through metabolic engineering techniques to provide new biocatalysts as well as the model based design of bioprocesses (up-stream processing, bioreactor operation and downstream processing) that will lead to new and sustainable production processes. Contributions are targeted at new approaches for rational and evolutive design of cellular systems by taking into account the environment and constraints of technical production processes, integration of recombinant technology and process design, as well as new hybrid intersections such as bioinformatics and process systems engineering. Manuscripts concerning the design, simulation, experimental validation, control, and economic as well as ecological evaluation of novel processes using biosystems or parts thereof (e.g., enzymes, microorganisms, mammalian cells, plant cells, or tissue), their related products, or technical devices are also encouraged. The Editors will consider papers for publication based on novelty, their impact on biotechnological production and their contribution to the advancement of bioprocess and biosystems engineering science. Submission of papers dealing with routine aspects of bioprocess engineering (e.g., routine application of established methodologies, and description of established equipment) are discouraged.
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