Lactobacillus delbrueckii ameliorates Aeromonas hydrophila-induced oxidative stress, inflammation, and immunosuppression of Cyprinus carpio huanghe var NF-κB/Nrf2 signaling pathway

IF 3.9 3区 环境科学与生态学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY Comparative Biochemistry and Physiology C-toxicology & Pharmacology Pub Date : 2024-08-13 DOI:10.1016/j.cbpc.2024.110000
Chunnuan Zhang , Changchang Pu , Shengnan Li , Ruiyi Xu , Qian Qi , Juan Du
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Abstract

Aeromonas hydrophila (A. hydrophila) is one of the most pathogenic disease-causing bacteria, and causes massive death of animals including fish. Thus, strategies are being sought to ameliorate the impact of A. hydrophila. In this study, we have evaluated the ameliorative potential of dietary Lactobacillus delbrueckii (L. delbrueckii). The fishes were divided into the control group, an A. hydrophila group (A. hydrophila), and an L. delbrueckii group (A. hydrophila + 1*107 CFU/g L. delbrueckii). The results showed that A. hydrophila increased reactive oxygen species (ROS) content. However, dietary supplementation with L. delbrueckii prevented oxidative damage caused by elevated levels of ROS. The toxic effects of A. hydrophila on superoxide dismutase (SOD) activity, glutathione-S-transferase (GST), glutathione peroxidase (GPx), and glutathione reductase (GR), along with the levels of glutathione (GSH), were mitigated by dietary L. delbrueckii (P < 0.05). Also, Dietary L. delbrueckii induced the expression of antioxidant-related genes (sod, cat, gpx, gst, NF-E2-related factor 2 (nrf2), Kelch-like-ECH-and associated protein 1a (keap1a)) in the intestine of fish (P < 0.05). Furthermore, L. delbrueckii increased A. hydrophila-induced lysozyme, ACP, C3, and C4 decline. The mRNA expression levels of interleukin 1β (il-1β), interleukin 8 (il-8), tumour necrosis factor α (tnf-α), and nuclear transcription factor-κB p65 (nf-κb p65) were significantly elevated by A. hydrophila. In contrast, the relative mRNA expression levels of inhibitor factor κBα (iκbα) in the intestine were decreased by A. hydrophila (P < 0.05). However, L. delbrueckii prevented A. hydrophila-induced the relative mRNA expression changes. These present results demonstrate that dietary L. delbrueckii alleviated A. hydrophila-induced oxidative stress, immunosuppression, inflammation, and apoptosis in common Cyprinus carpio.

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德尔布鲁贝克乳杆菌可改善嗜水气单胞菌诱导的氧化应激、炎症和鲤鱼黄鹤楼变种NF-κB/Nrf2信号通路的免疫抑制。
嗜水气单胞菌(A. hydrophila)是致病性最强的细菌之一,会导致包括鱼类在内的大量动物死亡。因此,人们一直在寻求改善嗜水气单胞菌影响的策略。在这项研究中,我们评估了饮食中的德尔布鲁贝克乳杆菌(L. delbrueckii)的改善潜力。鱼类被分为对照组、蚜茧蜂毒素组(蚜茧蜂毒素)和 delbrueckii 乳杆菌组(蚜茧蜂毒素 + 1*107 CFU/g delbrueckii 乳杆菌)。结果表明,嗜水蝇增加了活性氧(ROS)的含量。然而,膳食中补充 L. delbrueckii 可以防止 ROS 水平升高造成的氧化损伤。膳食中添加 L. delbrueckii(P<0.05)可减轻纤毛虫对超氧化物歧化酶(SOD)活性、谷胱甘肽-S-转移酶(GST)、谷胱甘肽过氧化物酶(GPx)和谷胱甘肽还原酶(GR)以及谷胱甘肽(GSH)水平的毒性影响。
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来源期刊
CiteScore
7.50
自引率
5.10%
发文量
206
审稿时长
30 days
期刊介绍: Part C: Toxicology and Pharmacology. This journal is concerned with chemical and drug action at different levels of organization, biotransformation of xenobiotics, mechanisms of toxicity, including reactive oxygen species and carcinogenesis, endocrine disruptors, natural products chemistry, and signal transduction with a molecular approach to these fields.
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