Studies on the antioxidant mechanisms of betacyanins from improved fermented red dragon fruit (Hylocereus polyrhizus) drink in HepG2 cells

IF 8.6 2区工程技术 Q1 ENERGY & FUELS Sustainable Materials and Technologies Pub Date : 2024-08-13 DOI:10.1016/j.susmat.2024.e01086

Teck Wei Lim , Renee Lay Hong Lim , Liew Phing Pui , Chin Ping Tan , Chun Wai Ho

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Abstract

Red dragon fruit (RDF, Hylocereus polyrhizus) is high in betacyanins content but is understudied for its antioxidant mechanisms in liver cells. The present study aimed to investigate the antioxidant mechanisms of the stabilised betacyanins in a novel RDF functional drink, Improved-FRDFD-dH₂O (produced from mild, sustainable approaches) using HepG2 cell line. Results revealed the betacyanins of Improved-FRDFD-dH₂O (12.5% and 25%, v/v) showed the highest direct scavenging effect on intracellular ROS from 75.39% to <31%. The antioxidant enzymes (SOD, CAT and GPx) activities in HepG2 cells were remarkably increased, with 12.5% and 25% Improved-FRDFD-dH₂O demonstrating the greatest enhancing effects. RT-qPCR proved the betacyanins of Improved-FRDFD-dH₂O possessed indirect antioxidant mechanisms, where the gene expressions associated with Nrf2-ARE pathway were upregulated by all the tested sample concentrations (highest fold: 6.82). Overall, the present findings highlighted the RDF's potential in functional product development with betacyanins as the prominent bioactive compound to provide direct and indirect antioxidative and hepatoprotective activities.

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改良发酵红火龙果（Hylocereus polyrhizus）饮料中的甜菜素在 HepG2 细胞中的抗氧化机制研究

红龙果（RDF，Hylocereus polyrhizus）含有大量的甜菜素，但其在肝细胞中的抗氧化机制却未得到充分研究。本研究旨在利用 HepG2 细胞系研究一种新型红火龙果功能饮料 Improved-FRDFD-dH2O（采用温和、可持续的方法生产）中稳定的甜菊糖苷的抗氧化机制。结果显示，改良型-FRDFD-dH2O（12.5% 和 25%，v/v）中的甜菜素对细胞内 ROS 的直接清除效果最高，从 75.39% 到 31%。HepG2 细胞中的抗氧化酶（SOD、CAT 和 GPx）活性显著提高，其中 12.5% 和 25% Improved-FRDFD-dH2O 的提高效果最好。RT-qPCR 证明了改良型-FRDFD-dH2O 的甜菜素具有间接抗氧化机制，在所有测试浓度的样品中，与 Nrf2-ARE通路相关的基因表达都得到了上调（最高倍数：6.82）。总之，本研究结果凸显了 RDF 在功能性产品开发方面的潜力，其主要生物活性化合物甜菜素具有直接和间接的抗氧化和保肝活性。

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来源期刊

Sustainable Materials and Technologies Energy-Renewable Energy, Sustainability and the Environment

CiteScore

13.40

自引率

4.20%

发文量

158

审稿时长

45 days

期刊介绍： Sustainable Materials and Technologies (SM&T), an international, cross-disciplinary, fully open access journal published by Elsevier, focuses on original full-length research articles and reviews. It covers applied or fundamental science of nano-, micro-, meso-, and macro-scale aspects of materials and technologies for sustainable development. SM&T gives special attention to contributions that bridge the knowledge gap between materials and system designs.