Identification and Function Analysis of Novel Hypoglycemic and Antioxidant Peptides from Chickpea.

IF 3.1 2区 农林科学 Q2 CHEMISTRY, APPLIED Plant Foods for Human Nutrition Pub Date : 2024-12-01 Epub Date: 2024-08-17 DOI:10.1007/s11130-024-01215-5
Pei Li, Guoping Chen, Rongyao Liang, Kaiwei Cai, Zihao Chen, Na Yang, Wenyi Huang, Zhiyong Xie, Yanlong Chen, Qiongfeng Liao
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Abstract

Chickpea is rich in protein and has been demonstrated to possess hypoglycaemic effects. However, the specific bioactive ingredients and mechanisms underlying their hypoglycaemic effects remain unclear. In this study, enzymatic hydrolysis and gel permeation chromatography were used to extract chickpea bioactive peptide (CBP) from chickpea protein. One of the products, CBP-75-3, was found to inhibit α-glucosidase (GAA) activity and significantly increase the viability of insulin resistant (IR) cells. Moreover, CBP-75-3 significantly increased the rate of glucose consumption and glycogen synthesis in IR-HepG2 cells. Moreover, CBP-75-3 decreased the levels of malondialdehyde and increased the levels of superoxide dismutase, glutathione, and glutathione peroxidase. Subsequently, 29 novel bioactive peptides in CBP-75-3 were identified by LC‒MS/MS, and the potential hypoglycaemic targets of these novel bioactive peptides were investigated using molecular docking. Based on the results, the residues of the novel bioactive peptides interact with GAA through hydrogen bonding (especially LLR, FH, RQLPR, KGF and NFQ by binding to the substrate binding pocket or the active centre of GAA), thereby inhibiting GAA activity and laying a foundation for its hypoglycaemic activity. In short, the novel bioactive peptides isolated and identified from chickpea can effectively exert hypoglycaemic effects and increase the antioxidant capacity of IR-HepG2 cells. This study reveals that CBP-75-3, a natural hypoglycaemic ingredient, has potential for applications in functional foods and provides a theoretical basis for the development and application of CBP in the future.

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鹰嘴豆中新型降糖和抗氧化肽的鉴定与功能分析
鹰嘴豆富含蛋白质,已被证实具有降血糖作用。然而,具体的生物活性成分及其降血糖作用的机制仍不清楚。本研究采用酶水解和凝胶渗透色谱法从鹰嘴豆蛋白中提取鹰嘴豆生物活性肽(CBP)。结果发现,CBP-75-3 能抑制α-葡萄糖苷酶(GAA)的活性,并显著提高胰岛素抵抗(IR)细胞的活力。此外,CBP-75-3 还能明显提高 IR-HepG2 细胞的葡萄糖消耗率和糖原合成率。此外,CBP-75-3 还能降低丙二醛的水平,提高超氧化物歧化酶、谷胱甘肽和谷胱甘肽过氧化物酶的水平。随后,通过 LC-MS/MS 鉴定了 CBP-75-3 中的 29 种新型生物活性肽,并利用分子对接法研究了这些新型生物活性肽的潜在降血糖靶点。结果表明,新型生物活性肽的残基与 GAA 通过氢键相互作用(尤其是 LLR、FH、RQLPR、KGF 和 NFQ 与 GAA 的底物结合口袋或活性中心结合),从而抑制了 GAA 的活性,为其降血糖活性奠定了基础。总之,从鹰嘴豆中分离鉴定出的新型生物活性肽能有效发挥降血糖作用,并提高 IR-HepG2 细胞的抗氧化能力。这项研究揭示了 CBP-75-3 这种天然降血糖成分在功能食品中的应用潜力,并为 CBP 未来的开发和应用提供了理论依据。
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来源期刊
Plant Foods for Human Nutrition
Plant Foods for Human Nutrition 工程技术-食品科技
CiteScore
6.80
自引率
7.50%
发文量
89
审稿时长
12-24 weeks
期刊介绍: Plant Foods for Human Nutrition (previously Qualitas Plantarum) is an international journal that publishes reports of original research and critical reviews concerned with the improvement and evaluation of the nutritional quality of plant foods for humans, as they are influenced by: - Biotechnology (all fields, including molecular biology and genetic engineering) - Food science and technology - Functional, nutraceutical or pharma foods - Other nutrients and non-nutrients inherent in plant foods
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