A sensitive tobramycin electrochemical aptasensor based on multiple signal amplification cascades

IF 4.8 2区 化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY Bioelectrochemistry Pub Date : 2024-08-15 DOI:10.1016/j.bioelechem.2024.108797
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Abstract

The residue of tobramycin, a broad spectrum antibiotic commonly used in animal husbandry, has evitable impact on human health, which may cause kidney damage, respiratory paralysis, neuromuscular blockade and cross-allergy in humans. Sensitive monitoring of tobramycin in animal-derived food products is therefore of great importance. Herein, a new aptamer electrochemical biosensor for sensing tobramycin with high sensitivity is demonstrated via exonuclease III (Exo III) and metal ion-dependent DNAzyme recycling and hybridization chain reaction (HCR) signal amplification cascades. Tobramycin analyte binds aptamer-containing hairpin probe to switch its conformation to expose the toehold sequence, which triggers Exo III-based catalytic digestion of the secondary hairpin to release many DNAzyme strands. The substrate hairpins immobilized on the Au electrode (AuE) are then cyclically cleaved by the DNAzymes to form ssDNAs, which further initiate HCR formation of lots of long methylene blue (MB)-tagged dsDNA polymers on the AuE. Subsequently electro-oxidation of these MB labels thus exhibit highly enhanced currents for sensing tobramycin within the 5–1000 nM concentration range with an impressive detection limit of 3.51 nM. Furthermore, this strategy has high selectivity for detecting tobramycin in milk and shows promising potential for detect other antibiotics for food safety monitoring.

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基于多重信号放大级联的灵敏妥布霉素电化学适配传感器
妥布霉素是一种常用于畜牧业的广谱抗生素,其残留对人体健康有一定的影响,可能会对人体造成肾脏损害、呼吸麻痹、神经肌肉阻滞和交叉过敏。因此,对动物源性食品中托布霉素的灵敏监测至关重要。本文通过外切酶 III(Exo III)和金属离子依赖性 DNA 酶循环和杂交链反应(HCR)信号放大级联,展示了一种新型适配体电化学生物传感器,可高灵敏度地检测妥布霉素。妥布霉素分析物与含aptamer的发夹探针结合,改变其构象以暴露趾hold序列,从而触发基于Exo III的二级发夹催化消化,释放出许多DNA酶链。然后,固定在金电极(AuE)上的底物发夹会被 DNA 酶循环裂解,形成 ssDNA,从而进一步在 AuE 上启动 HCR,形成大量亚甲基蓝(MB)标记的长 dsDNA 聚合物。随后,这些 MB 标签发生电氧化,从而显示出高度增强的电流,可在 5-1000 nM 浓度范围内感测妥布霉素,检测限为 3.51 nM,令人印象深刻。此外,这种方法对检测牛奶中的妥布霉素具有很高的选择性,并有望用于检测食品安全监控中的其他抗生素。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Bioelectrochemistry
Bioelectrochemistry 生物-电化学
CiteScore
9.10
自引率
6.00%
发文量
238
审稿时长
38 days
期刊介绍: An International Journal Devoted to Electrochemical Aspects of Biology and Biological Aspects of Electrochemistry Bioelectrochemistry is an international journal devoted to electrochemical principles in biology and biological aspects of electrochemistry. It publishes experimental and theoretical papers dealing with the electrochemical aspects of: • Electrified interfaces (electric double layers, adsorption, electron transfer, protein electrochemistry, basic principles of biosensors, biosensor interfaces and bio-nanosensor design and construction. • Electric and magnetic field effects (field-dependent processes, field interactions with molecules, intramolecular field effects, sensory systems for electric and magnetic fields, molecular and cellular mechanisms) • Bioenergetics and signal transduction (energy conversion, photosynthetic and visual membranes) • Biomembranes and model membranes (thermodynamics and mechanics, membrane transport, electroporation, fusion and insertion) • Electrochemical applications in medicine and biotechnology (drug delivery and gene transfer to cells and tissues, iontophoresis, skin electroporation, injury and repair). • Organization and use of arrays in-vitro and in-vivo, including as part of feedback control. • Electrochemical interrogation of biofilms as generated by microorganisms and tissue reaction associated with medical implants.
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